MiR-370-3p aggravates blood–brain barrier injury and neuron apoptosis by targeting SMURF1 to activate the TLR4/MyD88/NF-κB signaling in sepsis-associated encephalopathy

Wang Neng,Zhong Dan,Lin Jie,Ye Mei,Chen Yu,Wang Lili,Chen Mei,Luo Cong 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3

Background Sepsis-associated encephalopathy (SAE) is characterized by blood–brain barrier (BBB) disruption, neuron apoptosis and infl ammation. Studies show that miR-370-3p can be a new diagnostic biomarker for SAE. Objective This study aims to investigate the role of miR-370-3p in SAE and the underlying mechanism. Result An in vitro model of BBB disruption was established in brain microvascular endothelial cells (BMECs) with lipopolysaccharide (LPS). The expression level of RNAs was tested via RT-qPCR. Protein levels were detected by western blotting. Annexin V/PI double staining and CCK-8 assays were conducted to assess the apoptosis and viability of brain microvascular endothelial cells (BMECs). The migration of BMECs was detected by Transwell assay. BBB permeability analysis was performed to assess the relative permeability coeffi cient of BMECs. The in vitro barrier integrity was detected by trans-epithelial/ endothelial electrical resistance (TEER). The binding between miR-370-3p and SMAD specifi c E3 ubiquitin protein ligase 1 (SMURF1) was identifi ed by luciferase reporter assay. In this study, miR-370-3p was upregulated in LPS-treated BMECs. MiR-370-3p knockdown alleviated LPS-induced BBB disruption and apoptosis of BMECs, as well as recovered cell viability and migration. SMURF1 was directly targeted by miR-370-3p. SMURF1 silencing reversed the eff ects of miR-370-3p defi ciency in LPS-stimulated BMECs. Moreover, miR-370-3p activated the TLR4/MyD88/NF-κB signaling via regulating SMURF1 in LPS-stimulated BMECs. Conclusion MiR-370-3p aggravates BBB disruption and neuron apoptosis by targeting SMURF1 to activate the TLR4/ MyD88/NF-κB signaling in SAE.

Regulation of Antiarrhythmic Drug Propafenone Effects on the C-type KV1.4 Potassium Channel by PHo and K+

Zhiquan Wang,Jianjun Li,Xuejun Jiang,Xuejun Jiang,Neng Wang,Shimin Wang 대한의학회 2009 Journal of Korean medical science Vol.24 No.1

The effects of the antiarrhythmic drug propafenone at c-type kv1.4 channels in Xenopus laevis oocytes were studied with the two-electrode voltage-clamp techinique. Defolliculated oocytes (stage V-VI) were injected with transcribed cRNAs of ferret Kv1.4△N channels. During recording, oocytes were continuously perfused with control solution or propafenone. Propafenone decreased the currents during voltage steps. The block was voltage-, use-, and concentration- dependent manners. The block was increased with positive going potentials. The voltage dependence of block could be fitted with the sum of monoexponential and a linear function. Propafenone accelerated the inactivate of current during the voltage step. The concentration of half-maximal block (IC50) was 121 μM/L. With high, normal, and low extracellular potassium concentrations, the changes of IC50 value had no significant statistical differences. The block of propafenone was PH- dependent in high-, normal- and low- extracellular potassium concentrations. Acidification of the extracellular solution to PH 6.0 increased the IC50 values to 463 μM/L, alkalization to PH 8.0 reduced it to 58 μM/L. The results suggest that propafenone blocks the kv1.4 N channel in the open state and give some hints for an intracellular site of action.

Risk Factors of Postoperative Nosocomial Pneumonia in Stage I-IIIa Lung Cancer Patients

Wang, Zeng,Cai, Xin-Jun,Shi, Liang,Li, Fei-Yan,Lin, Neng-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: To investigate the related risk factors of postoperative nosocomial pneumonia (POP) in patients withI-IIIa lung cancer. Methods: Medical records of 511 patients who underwent resection for lung cancer between January 2012 to December 2012 were retrospectively reviewed. Risk factors of postoperative pneumonia were identified and evaluated by univariate and multivariate analyses. Results: The incidence of postoperative pneumonia in these lung cancer patients was 2.9% (15 cases). Compared with 496 patients who had no pneumonia infection after operation, older age (>60), histopathological type of squamous cell carcinoma and longer surgery time (>3h) were significant risk factors by univariate analysis. Other potential risk factors such as alcohol consumption, history of smoking, hypersensitivity, hypertension, diabetes mellitus and so on were not showed such significance in this study. Further, the multivariate analysis revealed that old age (>60 years) (OR 5.813, p=0.018) and histopathological type of squamous cell carcinoma (OR 5.831, p<0.001) were also statistically significant independent risk factors for postoperative pneumonia. Conclusions: This study demonstrated that being old aged (>60 years) and having squamous cell carcinoma histopathological type might be important factors in determining the risk of postoperative pneumonia in lung cancer patients after surgery.

Soil Carbon Cycle in Subtropical Afforestation in Taiwan

Po-Neng Chiang,Jui-Chu Yu,Ya-Nan Wang,Yen-Jen Lai 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Research on the dispersion of carbon nanotubes and their application in solution-processed polymeric matrix composites: A review

Feng, Tao,Liu, Neng,Wang, Shunjie,Qin, Can,Shi, Shengwei,Zeng, Xueying,Liu, Gang Techno-Press 2021 Advances in nano research Vol.10 No.6

This review highlights and categorizes the approaches for preparation of CNTs dispersion and polymer/CNTs composites via solution-based strategies. Carbon nanotubes (CNTs) demonstrate unique physical and chemical properties, which allow several exciting potential applications in various fields including nanocomposites. Presently, the commercialized application of CNTs is still quite limited due to the formation of CNTs bundles, which significantly degrade the properties. Therefore, well dispersion of CNTs in nanocomposites is quite important, especially for CNTs/polymer composites, as a small amount of CNTs can improve the composite properties dramatically. This article will review the research on the dispersion of CNTs (including covalent and non-covalent functionalization) and the fabrication of CNTs/polymer composites through solution-based strategies by using the CNT dispersions. Moreover, the factors influencing the properties of CNTs/polymer composites will be discussed as well as the future outlook.

Theoretical and Exprerimental Sutdy on Interactive Local Buckling of Arch-shaped Corrugated Steel Roof

Li-Li Wu,Xuan-Neng Gao,Yong-Jiu Shi,Yuan-Qing Wang 한국강구조학회 2006 International Journal of Steel Structures Vol.6 No.3

The arch-shaped corugated steel roof is a new type of cold formed thin-waled structure. Since the width-to-thickness ratiosof the plate asemblies of corrugated sections exceed the limits specified in the current design codes, the load-bearingbehavior, especialy loading modes, initial imperfections and boundary conditions, therefore its ultimate load-carying capacityis determined not only by its global stability, but also by its interactive local buckling. In this paper the finite element methodwas aplied to analyze the local buckling behavior of stel arch panels with corrugated trapezium sections. Some tests on theloads to further investigate the loading mechanism of corrugated steel arch panels and the factors refered above. A comparisonbetween the experimental and theoretical results was made, and good agrement was achieved herein. It is shown that therealso exists interactive local buckling among corugated plate assemblies. The experimental results are lower than theoreticalones for axial loading capacity, vice-versa, the former ones are higher for eccentricaly loaded specimens. It is noted that thealso indicate that the trapezium sections have high local buckling capacities, yet they hardly have post-buckling capacities,which are distinctive from that of the plain ones. All these also give some explanations to some corugated steel arch rofscolapsed suddenly in some areas of China.

Tailoring fabric geometry of plain-woven composites for simultaneously enhancing stiffness and thermal properties

Xiao-Yi Zhou,Neng-Wei Wang,Wen Xiong,Xin Ruan,Shao-Jin Zhang 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.42 No.4

This paper proposes a numerical optimization method to design the mesoscale architecture of textile composite for simultaneously enhancing mechanical and thermal properties, which compete with each other making it difficult to design intuitively. The base cell of the periodic warp and fill yarn system is served as the design space, and optimal fibre yarn geometries are found by solving the optimization problem through the proposed method. With the help of homogenization method, analytical formulae for the effective material properties as functions of the geometry parameters of plain-woven textile composites were derived, and they are used to form the inverse homogenization method to establish the design problem. These modules are then put together to form a multiobjective optimization problem, which is formulated in such a way that the optimal design depends on the weight factors predetermined by the user based on the stiffness and thermal terms in the objective function. Numerical examples illustrate that the developed method can achieve reasonable designs in terms of fibre yarn paths and geometries.

Aberrant Expression of the Autocrine Motility Factor Receptor Correlates with Poor Prognosis and Promotes Metastasis in Gastric Carcinoma

Huang, Zhen,Zhang, Neng,Zha, Lang,Mao, Hong-Chao,Chen, Xuan,Xiang, Ji-Feng,Zhang, Hua,Wang, Zi-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

AMFR, autocrine motility factor receptor, also called gp78, is a cell surface cytokine receptor which has a dual role as an E3 ubiquitin ligase in endoplasmic reticulum-associated degradation. AMFR expression is associated with tumor malignancy. We here investigated the clinical significance of AMFR and its role in metastasis and prognosis in gastric cancer. Expression of AMFR, E-cadherin and N-cadherin in cancer tissues and matched adjacent normal tissues from 122 gastric cancer (GC) patients undergoing surgical resection was assessed by immunohistochemistry. Levels of these molecules in 17 cases selected randomly were also analysed by Western blotting. AMFR expression was significantly increased in gastric cancer tissues, and associated with invasion depth and lymph node metastasis. Kaplan-Meier analysis showed AMFR expression correlated with poor overall survival and an increased risk of recurrence in the GC cases. Cox regression analysis suggested AMFR to be an independent predictor for overall and recurrence-free survival. E-cadherin expression was decreased in gastric cancer tissues; conversely, N-cadherin was increased. Expression of AMFR negatively correlated with E-cadherin expression, whereas N-cadherin expression showed a significant positive correlation with AMFR expression. AMFR might be involved in the regulation of epithelial-mesenchymal transition, with aberrant expression correlating with a poor prognosis and promoting invasion and metastasis in GCs.

Genetic Diversity of a Novel Oil Crop, Camellia brevistyla, Revealed by ISSR DNA Markers

Mong-Huai Su,Te-Hua Hsu,Chun-Neng Wang,Kuan-Hung Lin,Ming-Chang Chiang,Ren-Dong Kang,Khiem M. Nguyen,Hoang Nguyen 한국원예학회 2017 원예과학기술지 Vol.35 No.5

Camellia brevistyla plants are used as food oil in Taiwan. To provide a method for identifying germplasm and assessing the genetic diversity of C. brevistyla , 102 individuals were collected from 29 populations in 11 counties throughout the main island of Taiwan. Inter-simple sequence repeat (ISSR) DNA markers were analyzed and a total of 176 bands were amplified. Of these, 175 bands were found to be polymorphic in these populations. Genetic similarities among populations ranged between 34.1-95.1%. Cluster analysis revealed seven groups of populations plus one outlier (C. kissii ). Five individuals from two populations in the southern region were grouped into cluster V, and six populations from both regions were grouped into cluster VII. The remaining populations from the northern region were separated into different clusters from V and VII. Results of an analysis of molecular variance indicated that the variation within populations (75%) was predominantly greater than variations among counties (18%) and among regions (7%). Overall, the gene flow (Nm = 0.509) estimated from genetic differentiation (Gst = 0.534) suggested that gene flow among regions was limited. Higher genetic diversity (H = 0.317), Shannon’s index (I = 0.477), and gene flow (Nm = 0.538) of populations were found in the northern region compared to the southern region. The DNA sequences of C. brevistyla amplified by two oil-biosynthesis genes showed 99-100% homology with those of C. oleifera. Camellia brevistyla populations are highly differentiated and can serve as a basis for the food oil industry as a germplasm resource.

Axial slit wall effect on the flow instability and heat transfer in rotating concentric cylinders

Dong Liu,Chang-qing Chao,Ying-ze Wang,Fang-neng Zhu,김형범 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.12

The slit wall effect on the flow instability and heat transfer characteristics in Taylor-Couette flow was numerically studied by changing the rotating Reynolds number and applying the negative temperature gradient. The concentric cylinders with slit wall are seen in many rotating machineries. Six different models with the slit number 0, 6, 9, 12, 15 and 18 were investigated in this study. The results show the axial slit wall enhances the Taylor vortex flow and suppresses the azimuthal variation of wavy Taylor vortex flow. When negative temperature gradient exists, the results show that the heat transfer augmentation appears from laminar Taylor vortex to turbulent Taylor flow regime. The heat transfer enhancement become stronger as increasing the Reynolds number and slit number. The larger slit number model also accelerates the flow transition regardless of the negative temperature gradient or isothermal condition.