Intestine epithelial cell-derived extracellular vesicles alleviate inflammation induced by Clostridioides difficile TcdB through the activity of TGF-β1

Wan Shuangshuang,Song Guangzhong,Hu Hui,Xu Yaqing,Zeng Peng,Lin Shan,Yang Jun,Jiang Jinqin,Song Xiaojun,Luo Yongneng,Jin Dazhi 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3

Background Clostridioides diffi cile infection (CDI) has been primarily associated with the toxin B (TcdB), one of the three known protein toxins secreted by C. diffi cile , which can activate the intestinal immune system and lead to pathological damage. Even though the biological functions of intestine epithelial cell-derived extracellular vesicles (I-Evs) have been well documented, the role of I-Evs in the process of CDI is still unknown. Objectives The protective eff ect of I-Evs against C. diffi cile TcdB was investigated both in cultured murine colon carcinoma MC38 cells and a mouse model used in this study. Results Mouse I-Evs with mean diameter ranging from 100 to 200 nm and a density of 1.09–1.17 g/mL were obtained and confi rmed containing the Ev-associated specifi c surface markers CD63 and TSG101 as well as high level of TGF-β1. In MC38 cells, I-Evs were able to decrease the gene expression of IL-6, TNF-α, IL-1β, and IL-22 induced by C. diffi cile TcdB, but to increase both the gene expression and protein levels of TGF-β1. I-Evs treatment via intraperitoneal administration alleviates C. diffi cile TcdB-induced local colon infl ammation in mice and increased their survival rate from 50% up to 80%. Furthermore, I-Evs induced an increase in the proportion of CD4 + Foxp3 + Tregs in vitro and in vivo through a TGF-β1-dependent mechanism by activating the TGF-β1 pathway and prompting phosphorylation of the downstream proteins Smad 2/3. Conclusion For the fi rst time, our study demonstrated that I-Evs originated from intestine epithelial cells can alleviate infl ammation induced by C. diffi cile TcdB both in vitro and in vivo. Therefore, I-Evs might be potentially a novel endogenous candidate for eff ective treatment of CDI.

Reduction of Physical Strength and Enhancement of Anti-Protein and Anti-Lipid Adsorption Abilities of Contact Lenses by Adding 2-Methacryloyloxyethyl Phosphorylcholine

Wan-Hsin Chang,Pei-Yi Liu,Chien-Ju Lu,Dai-En Lin,Min-Hsuan Lin,Yuan-Ting Jiang,Yuan-Hao Howard Hsu 한국고분자학회 2020 Macromolecular Research Vol.28 No.12

Biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC) can enhance the adsorption of water molecules and is therefore used for manufacturing contact lenses. This study investigated the mechanical strength, anti-protein deposition, and anti-lipid adsorption effects of MPC addition to contact lenses. Experimental contact lenses produced by copolymerizing multiple ratios of MPC to 2-hydroxyethyl methacrylate (HEMA) were analyzed. Atomic force microscopy revealed that MPC addition increased surface roughness. The anti-protein deposition and anti-lipid adsorption effects on poly(HEMA-MPC) polymers of various phosphorylcholine quantities were experimentally confirmed. The water content of the contact lenses was proportional to the MPC content in the polymer. The hydrated PC moiety of MPC drastically altered the network of the poly-HEMA polymer by inserting water molecules, which were trapped in the concave region of the surface. MPC addition had negative effects on all examined strength factors because of structural destabilization of the copolymer through water insertion. The anti-deposition effects of MPC were verified by examining the lysozyme and lipid adsorption abilities of the prepared contact lenses. Our results revealed that MPC enhanced interactions of the poly(HEMA-MPC) copolymer with water molecules; these interactions weakened the mechanical strength of the copolymer but markedly improved the anti-adsorption property of the biomolecules. The optimal proportion of HEMA–MPC for contact lenses is in the range 14.9%-28.5%.

Dynamic Protein Adsorption-Desorption Analysis of Contact Lenses in a Three-Dimensional-Printed Eye Model

Wan-Hsin Chang,Pei-Yi Liu,Dai-En Lin,Yuan-Ting Jiang,Chien-Ju Lu,Yuan-Hao Howard Hsu 한국고분자학회 2022 Macromolecular Research Vol.30 No.1

Adsorption of secreted protein on contact lenses is a dynamic and complex behavior. To understand this behavior, we used three-dimensional (3D) printing technology to create an eye model that simulated the anterior segment of the actual human eyeball. In this model, the fluid inlet was connected to a syringe pump to mimic the rate of human tear secretion and the outlet was connected to an ultraviolet- visible (UV-Vis) spectrophotometer. The experimental results revealed that the symmetrical eye model with a 180° inlet-outlet angle was suitable for dynamic analysis of protein adsorption. In this model, protein adsorption was slow and desorption was rapid. The contact lens was soaked in poly(2-methacryloyloxyethyl phosphorylcholine- co-butyl methacrylate) (PMB) to confirm the anti-protein adsorption property of this polymer through dynamic adsorption and desorption eye model analysis.

Digital Image-based Identification Method for the Determination of the Particle Size Distribution of Dam Granular Material

Shi-lin Zhang,Gao-jian Wu,Xingguo Yang,Wan-hong Jiang,Jia-wen Zhou 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.8

The Particle Size Distribution (PSD) properties of dam granular material plays an important role in the construction process ofearth-rock dams, as it can affect the filling quality and structural safety. However, the conventional sieving method employed tocheck the PSD is labor-intensive, time-consuming and not highly accurate. In this study, a digital image-based identification methodis presented for the determination of the PSD of dam granular material, which mainly incorporates image acquisition technology, alarge database and a neural network. Digital Image Processing (DIP) technology is used to recognize the geometric size and gradingcurve of dam granular materials at a small scale, while statistical distribution models are used to determine the characteristicparameters of the grading curve and convert the graphical curve into mathematical variables. Furthermore, a large database and a BPneutral algorithm, which is improved using a genetic algorithm, are introduced as tools to reveal the implicit relationship between theDIP and sieving grading curves to correct the error of identification. A case study for the Changheba Hydropower Station is used toillustrate the implementation details of the presented method. The identification results demonstrate that the presented method canacquire and assess the gradation in spite of a degree of error, which can be decreased when more advanced DIP technologies areexplored, the amount of data in the database is increased, and a more optimized network algorithm is adopted.

Cryptanalysis of a Biometric-based Multi-Server Authentication Scheme

Tao Wan,Nan Jiang,Jianfeng Ma,Lin Yang 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.2

Authentication and key agreement protocol becomes an important security issue for multi-server architecture. Combining biometrics with password enhances the level of security. Recently, Baruah et al. analyzed that Mishra et al.’s protocol has several drawbacks and proposed an improved biometric based multi-server authentication scheme. They claimed that their scheme satisfies all the required security attributes for a secure authentication. In this paper, we indicate that their scheme is not secure against key reveal attack, replay attack, and smart card forgery attack. Any registered user can retrieve the session key or launch the replay attack by eavesdropping on the communication channel. In addition, registered user can forge smart card when colluding with registered server.

Rhaponticin suppresses the hypoxia-induced factor-1 alpha-mediated aggressive phenotype of tongue squamous cell carcinoma

Wu Yuan,Wan Xiaowen,Shao Yisen,Wang Wei,Huang Wenquan,Zhu Jiajun,Jiang Lin 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.2

Emerging evidence suggests that rhaponticin, a stilbene monomeric compound isolated from North China rhubarb, has been shown to exhibit significant biological activity against tumors. However, the anticancer effects and mechanisms of rhaponticin in tongue squamous cell carcinoma (TSCC) remain elusive.We investigated the changes of migration and invasion abilities and EMT progression of TSCC cells treated with different concentrations of rhaponticin under hypoxia, as well as the possible mechanisms, in order to initially explore the effects of rhaponticin on the biological characteristics of TSCC cells under hypoxia.The number of cell migration and invasion was prominently increased, E-cadherin protein was down-regulated, and N-cadherin and HIF-1α protein expression was elevated under hypoxia. Rhaponticin intervention strikingly prevented the increased abilities of migration and invasion and EMT of TSCC cells under hypoxia. This was followed by further validation finding that rhaponticin indeed leads to reduced HIF-1α post-transcriptional activity. Mechanistically, rhaponticin may bind to aryl-hydrocarbon nuclear translocator (ARNT) domain of HIF-1α.Rhaponticin repressed the invasion and migration abilities and EMT process of TSCC cells under a hypoxic environment in vitro by targeted suppression of HIF-1α. Background Emerging evidence suggests that rhaponticin, a stilbene monomeric compound isolated from North China rhubarb, has been shown to exhibit significant biological activity against tumors. However, the anticancer effects and mechanisms of rhaponticin in tongue squamous cell carcinoma (TSCC) remain elusive. Objective We investigated the changes of migration and invasion abilities and EMT progression of TSCC cells treated with different concentrations of rhaponticin under hypoxia, as well as the possible mechanisms, in order to initially explore the effects of rhaponticin on the biological characteristics of TSCC cells under hypoxia. Results The number of cell migration and invasion was prominently increased, E-cadherin protein was down-regulated, and N-cadherin and HIF-1α protein expression was elevated under hypoxia. Rhaponticin intervention strikingly prevented the increased abilities of migration and invasion and EMT of TSCC cells under hypoxia. This was followed by further validation finding that rhaponticin indeed leads to reduced HIF-1α post-transcriptional activity. Mechanistically, rhaponticin may bind to aryl-hydrocarbon nuclear translocator (ARNT) domain of HIF-1α. Conclusions Rhaponticin repressed the invasion and migration abilities and EMT process of TSCC cells under a hypoxic environment in vitro by targeted suppression of HIF-1α.

Design and Implementation on Spatial Science and Technology Information Database of CSI

Chen, Xiu Wan,Deng, Zheng Quan,Lu, Zhi Gao,Ma, Jia,Lin, Jia Yuan,Zhang, Wen Jiang,Luo, Tianfu,Liu, Baofu 대한원격탐사학회 2000 International Symposium on Remote Sensing Vol.16 No.1

Remote Sensing technology, which is characterized by producing imagery an multi-platform, different temporal and spatial resolution, has greatly improved mankind's capability of acquisition, processing and application of spatial information. The increase of spatial data sources and the development, applications and industrialization of spatial information technology are urging the need of spatial data sharing and exchanging. Based an a brief introduction an the China Spatial Information Network (CSI) and its database system, the CSI Spatial Science and Technology Information Database (SSTID) management system was designed and implemented in this paper.

Novel Cationic Polymers Designed for Non-Viral Gene Delivery

Zhiyuan Zhong,Chao Lin,Yan Song,Martin C. Lok,Xulin Jiang,Lane V. Christensen,Johan F.J. Engbersen,Sung Wan Kim,Wim E. Hennink,Jan Feijen 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.10

Grenville-age orogenic event along the northeastern margin of the Quanji massif, NW China: constraints from ~1.1 Ga migmatite

Zhouxuan Xiao,Licheng Ma,Wan Jiang,Ran Wang,Yuyan Yao,Jie Cai,Jingyu Lin,Lu Li 한국지질과학협의회 2020 Geosciences Journal Vol.24 No.3

Detailed petrologic, geochemical and geochronological investigation on migmatites provide key information on the tectonic-magmatic history of the Quanji Massif, NW China. The studied migmatites are mainly composed of granitic gneisses, with lenticular refractory residua spread along its foliation. Zircon U-Pb ages of 1129.2 ± 7.4 Ma (1σ, MSWD = 0.34, N = 31) and 1135.1 ± 9.5 Ma (1σ, MSWD = 0.05, N = 22) were determined for the granitic gneiss and refractory residue, respectively. Garnet grains from the refractory residue show no distinctive intra-crystalline zonation, and are rich in Fe and Mg, but poor in Ca and Mn, akin to those from metapelite. Samples from the granitic gneisses have high contents of SiO2 (from 67.92% to 69.89%), K2O (from 3.94% to 5.00%) and A/CNK (1.11–1.17), with LREE enrichment and distinctly negative Eu anomalies (Eu/Eu* = 0.49–0.53) in the chondrite-normalized REE patterns, and significantly negative Nb, Ta, Ti and Sr anomalies in the primitive mantle-normalized spider diagrams. Granitic gneisses samples have consistent Nd isotope compositions with negative εNd(t) values ranging from –1.06 to –6.04, and TDM model ages ranging from 1.83 to 2.23 Ga. These geochemical features suggest that the granitic rocks belong to the S-type granites, which were predominately derived from a late Paleoproterozoic crustal source. The granitic gneiss was probably formed by partial melting of the sediments which had been suffered graunlite-facies metamorphism. The components with low melting points (e.g., felsic minerals) might replace the sediments to form migmatites and migmatitic granites, and thus preserved the refractory residua. Therefore, the late Mesoproterozoic orogenic event is the coeval response of the global Grenvillian orogeny, indicating an important crustal remelting episode in the Quanji Massif.

Efficacy of and Patient Compliance with a Ketogenic Diet in Adults with Intractable Epilepsy: A Meta-Analysis

Fang Ye,Xiao-Jia Li,Wan-Lin Jiang,Hong-Bin Sun,Jie Liu 대한신경과학회 2015 Journal of Clinical Neurology Vol.11 No.1

Background and Purpose Despite the successful use of a ketogenic diet in pediatric epilepsy, its application in adults has been limited. The aim of this meta-analysis was to summarize thefindings of relevant published studies in order to identify the efficacy of and compliance with aketogenic diet and its main subtypes (i.e., classic ketogenic diet and modified Atkins diet) inadults with intractable epilepsy, and to provide useful information for clinical practice. Methods Electronic searches of PubMed, EMBASE, Google Scholar, and the ISI Web of Science were conducted to identify studies of the efficacy of and patient compliance with a ketogenic diet in adults with intractable epilepsy; the included studies were reviewed. Meta-analyseswere performed using STATA to determine combined efficacy rates and combined rates of compliance with the ketogenic diet and its main subtypes. Results In total, 12 studies qualified for inclusion, and data from 270 patients were evaluated. The results of the meta-analysis revealed combined efficacy rates of all types of ketogenic diet,a classical ketogenic diet, and a modified Atkins diet were 42%, 52%, and 34%, respectively;the corresponding combined compliance rates were 45%, 38%, and 56%. Conclusions The results indicate that a ketogenic diet is a promising complementary therapyin adult intractable epilepsy, and that while a classical ketogenic diet may be more effective,adult patients are likely to be less compliant with it than with a modified Atkins diet.