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Hui-Chao Hu,Hui-Jun Jin,Xin-Sheng Chai 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.1
This paper reports an improved headspace gas chromatographic (HS-GC) technique for thedetermination of oxalate content in bleaching effluents. The method is based on the redox reactionbetween potassium iodate and oxalate, from which the carbon dioxide is generated and measured by HSGC. The results showed that the reaction in a sulfuric acid solution (0.05 mol/L) was complete in 35 minat 95 ℃. The RSD of the method in the repeatability testing was less than 4.5%, the LOQ was 0.35 mg, andthe recovery ranged from 95 to 103%. The present method is simpler, more reliable, and more practicalcompared to the methods reported previously.
( Hui Jing ),( You Jin Dai ),( Yuan Yuan Bian ),( Hui Li ),( Xiao Jin Cui ),( Xiao Jie Yu ),( Song You ),( Feng Qing Hu ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.7
Based on their respective antitumor and thrombolytic activities, the superantigen staphylococcal enterotoxin C2 (SEC2) and staphylokinase (Sak) were chosen for the construction of the novel chimeric proteins Sak-linker- SEC2 and SEC2-linker-Sak using a linker composed of nine Ala residues. Both chimeric proteins possessed nearly the same PBMC proliferation stimulating activity and antitumor activity as SEC2 and thrombolytic activity as Sak. Neither the SEC2 or Sak component of each chimeric protein affected the activity of the other component. The results presented in this study provide a possible strategy to prevent and cure tumor thrombus.
Hu, Zhi-Bin,Luo, He-An,Wang, Xiao-Guang,Huang, Ming-Zhi,Huang, Lu,Pang, Huai-Lin,Mao, Chun-Hui,Pei, Hui,Huang, Chao-Qun,Sun, Jiong,Liu, Ping-Le,Liu, Ai-Ping Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.4
In attempt to lead compounds exhibiting both insecticidal and fungicidal activities, a series of O-benzyl oximeether derivatives were designed and synthesized by introducing ${\beta}$-methoxyacrylate pharmacophore into a scaffold. The insecticidal activity against Aphis fabae and the fungicidal activity against Erysiphe graminis were screened. The title compounds exhibited remarkable insecticidal and fungicidal activities. The most potent compound 6d was identified. Its insecticidal $LC_{50}$ against A. fabae is 6.4 mg/L, which is lower than that of chlorfenapyr (19.4 mg/L) and even close to the level of imidacloprid (4.8 mg/L). Its fungicidal $EC_{90}$ in preventive and curative treatment against E. graminis are 2.2 and 4.8 mg/L, respectively, which are lower than azoxystrobin (7.0 and 5.9 mg/L). These results indicate that compound 6d can be considered as a lead for further developing new O-benzyl oxime-ether typed candidates with both fungicidal and insecticidal activities.
Hu, Zheng-Hui,Lin, Yi-Wei,Xu, Xin,Chen, Hong,Mao, Ye-Qing,Wu, Jian,Xu, Xiang-Lai,Zhu, Yi,Li, Shi-Qi,Zheng, Xiang-Yi,Xie, Li-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Objective: To evaluate the association between tea consumption and the risk of renal cell carcinoma. Methods: We searched PubMed, Web of Science and Scopus between 1970 and November 2012. Two evaluators independently reviewed and selected articles based on predetermined selection criteria. Results: Twelve epidemiological studies (ten case-control studies and two cohort studies) were included in the final analysis. In a meta-analysis of all included studies, when compared with the lowest level of tea consumption, the overall relative risk (RR) of renal cell carcinoma for the highest level of tea consumption was 1.03 (95% confidence interval [CI] 0.89-1.21). In subgroup meta-analyses by study design, there was no significant association between tea consumption and renal cell carcinoma risk in ten case-control studies using adjusted data (RR=1.08, 95% CI 0.84-1.40). Furthermore, there was no significant association in two cohort studies using adjusted data (RR=0.95, 95% CI 0.81-1.12). Conclusion: Our findings do not support the conclusion that tea consumption is related to decreased risk of renal cell carcinoma. Further prospective cohort studies are required.
Proper Fhl1 expression as Wnt signaling is required for chondrogenesis of ATDC5 cells
Hu-Hui Lee,Jing-Yu Lee,Lan-Hsin Shih 한국통합생물학회 2013 Animal cells and systems Vol.17 No.6
Wnt signaling is well known playing dual roles during chondrogenesis. The overexpression of Fhl1 (Four-and-a-half LIM domain 1) induces myotube formation as a downstream event of Wnt signaling. Because Fhl1 is widely expressed in other mesenchyme-derived tissues, including chondrocytes, we investigated the role of Fhl1 in chondrogenesis and its relationship with Wnt signaling. We found that the expression of Fhl1 can be enhanced by β-catenin and LiCl (Lithium chloride) in chondrogenic ATDC5 cells. Overexpression of Fhl1 as well as canonical Wnt signaling inhibits chondrogenesis of ATDC5 cells. Moreover, shRNA-mediated knockdown of Fhl1 expression also inhibited ATDC5 cell differentiation, and this result is resembled to the mutant phenotype caused by deletion of β-catenin as was described previously. Because the endogenous Fhl1 expression remains constant during the middle and late phases of chondrogenesis, we propose that proper Fhl1 expression is necessary for the chondrogenic differentiation of ATDC5 cells and altered Fhl1 expression serves as an aberrant Wnt signal that impedes chondrogenesis.
Embryoid bodies formation from chicken primordial germ cells
Hui Xiong,Yabin Pu,Qingyun Hu,Zhiqiang Shan,Pengfei Hu,Weijun Guan,Yuehui Ma 한국통합생물학회 2015 Animal cells and systems Vol.19 No.3
Primordial germ cells (PGCs) were demonstrated to be multipotential because of their differentiation ability in all embryonic lineages and because the pluripotential nature of PGCs caters for recent researches on stem cells. PGCs were cultured in suspension to form embryoid bodies (EBs). The characterization of PGCs and EBs was assessed by immunofluorescence technique, reverse transcription-polymerase chain reaction (RT-PCR) and paraffin section for Haematoxylin and Eosin (HE) stain. We established a stable chicken PGC line in vitro and prepared masses of EBs from PGCs. We also demonstrated that PGCs expressed stage-specific and stem cell-specific surface makers: SSEA-1, SSEA-3, Oct4, and Sox2. EBs expressed specific genes from three germ layers: gene AFP from entoderm, gene GATA6 from mesoderm, and gene Sox3 from ectoderm. The HE staining illustrated that EBs developed different cell types; relatively larger EBs (440 μm in diameter) were obtained, which contract rhythmically. We concluded that chicken PGCs were suitable for the formation of EBs. Abundant larger size EBs could be prepared in an effective way with our protocol, which could construct a good animal model and provide a useful clinical platform in studying human embryonic development and cell transplantation field.
Hui-Chao Hu,Xin-Sheng Chai,Ying-Xin Tian,Wei-Feng Si,Gang Chen 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.3
This paper reports on a headspace gas chromatographic method (HS-GC) for the determination of residual formaldehyde in formaldehyde related polymer latexes. The method is based on the reaction between formaldehyde and borohydride in a sodium hydroxide solution (1 mol/L), in which formaldehyde is quantitatively converted to methanol within 30 min at 90 8C and then determined by HS-GC. The results showed that the repeatability of the method had a relative standard deviation of less than 5.0%; the limit of quantification (LOQ) was 17.3 mg, and the recovery ranged from 96.2–102%. The present method is simple, rapid, and accurate.
Hu, Zheng-Hui,Lin, Yi-Wei,Xu, Xin,Chen, Hong,Mao, Ye-Qing,Wu, Jian,Zhu, Yi,Xu, Xiang-Lai,Xie, Li-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1
Background: Many studies have investigated associations between the glutathione S-transferase M1 (GSTM1) null polymorphism and risk of prostate cancer, but the impact of GSTM1 in people who live in Asian countries is still unclear owing to inconsistencies across results. Methods: We searched the PubMed, Web of Science, Scopus, Ovid and CNKI databases for studies of associations between the GSTM1 null genotype and risk of prostate cancer in people who live in Asian countries, and estimated summary odds ratios (ORs) with 95% confidence intervals (95% CIs). Results: A total of 18 case-control studies with 2,172 cases and 3,258 controls were included in this meta-analysis, which showed the GSTM1 null genotype to be significantly associated with increased risk of prostate cancer in people who live in Asian countries (random-effects OR=1.74, 95% CI1.44-2.09, P<0.001). Similar results were found in East Asians (OR=1.41; 95% CI: 1.12-1.78; P=0.004) and Caucasians in Asia (OR=2.19; 95% CI: 1.85-2.60; P<0.001). No evidence of publication bias was observed. Conclusions: This meta-analysis of available data suggested that the GSTM1 null genotype does contribute to increased risk of prostate cancer in people who live in Asian countries.