An integrated view of lipid metabolism in ferroptosis revisited via lipidomic analysis

Kim Jong Woo,Lee Ji-Yoon,Oh Mihee,Lee Eun-Woo 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Ferroptosis is a form of regulated cell death characterized by iron-dependent lipid peroxidation. This process contributes to cellular and tissue damage in various human diseases, such as cardiovascular diseases, neurodegeneration, liver disease, and cancer. Although polyunsaturated fatty acids (PUFAs) in membrane phospholipids are preferentially oxidized, saturated/monounsaturated fatty acids (SFAs/MUFAs) also influence lipid peroxidation and ferroptosis. In this review, we first explain how cells differentially synthesize SFA/MUFAs and PUFAs and how they control fatty acid pools via fatty acid uptake and β-oxidation, impacting ferroptosis. Furthermore, we discuss how fatty acids are stored in different lipids, such as diacyl or ether phospholipids with different head groups; triglycerides; and cholesterols. Moreover, we explain how these fatty acids are released from these molecules. In summary, we provide an integrated view of the diverse and dynamic metabolic processes in the context of ferroptosis by revisiting lipidomic studies. Thus, this review contributes to the development of therapeutic strategies for ferroptosis-related diseases.

Constituents from the stems of Parthenocissus tricuspidata and their DNA topoisomerases I andⅡinhibition and cytotoxicity

Woo MiHee,Cao LiHua,Fang Zhe,Jung JiHyun,Lee WiJea,Jung EunJoo,choi DooHyung,Son JongKeun,Lee ChongSoon 대한약학회 2004 大韓藥學會 總會 및 學術大會 Vol.2004 No.2

Anticancer constituents with topoisomerase inhibitory activity from the roots of Rubia cordifolia L.(Ⅱ)

Woo MiHee,Jung SoonJa,Jung JiHyun,Fang Zhe,Lee WiJae,Jung EunJoo,Choi DooHyung,Son JongKeun,Lee ChongSoon 대한약학회 2004 大韓藥學會 總會 및 學術大會 Vol.2004 No.2

Bioactive constituents from the leaves of Zanthoxyhim schinifolium.

WOO MIHEE,LEE CH0NGS00N,SON JONG KEUN,JEONG SU YANGK,FANG ZHE 대한약학회 2006 大韓藥學會 總會 및 學術大會 Vol.2006 No.2

Depletion of Janus kinase-2 promotes neuronal differentiation of mouse embryonic stem cells

Mihee Oh,Sun Young Kim,Jeong-Su Byun,Seonha Lee,Won-Kon Kim,Kyoung-Jin Oh,Eun-Woo Lee,Kwang-Hee Bae,이상철,Baek-Soo Han 생화학분자생물학회 2021 BMB Reports Vol.54 No.12

Janus kinase 2 (JAK2), a non-receptor tyrosine kinase, is a criticalcomponent of cytokine and growth factor signaling pathwaysregulating hematopoietic cell proliferation. JAK2 mutations areassociated with multiple myeloproliferative neoplasms. Althoughphysiological and pathological functions of JAK2 in hematopoietictissues are well-known, such functions of JAK2 in thenervous system are not well studied yet. The present study demonstratedthat JAK2 could negatively regulate neuronal differentiationof mouse embryonic stem cells (ESCs). Depletion ofJAK2 stimulated neuronal differentiation of mouse ESCs andactivated glycogen synthase kinase 3ꞵ, Fyn, and cyclin-dependentkinase 5. Knockdown of JAK2 resulted in accumulation of GTPboundRac1, a Rho GTPase implicated in the regulation of cytoskeletaldynamics. These findings suggest that JAK2 might negativelyregulate neuronal differentiation by suppressing the GSK-3β/Fyn/CDK5 signaling pathway responsible for morphological maturation.

개인-직무 적합성과 과업 및 맥락 성과

김미희(Mihee Kim),신유형(Yuhyung Shin),문철우(Chul Woo Moon) 한국인사조직학회 2012 인사조직연구 Vol.20 No.1

개인-직무 적합성에 대한 연구가 오랫동안 지속되어 왔음에도 불구하고 개인-직무 적합성과 직무 성과간의 관계에서 조절효과를 미치는 변수들에 대한 규명은 최근에 와서야 이루어졌다. 본 연구에서는 팀제와 임파워먼트의 확산 등으로 대표 되는 최근의 경영 흐름에 따라 직무특성이론이 제시하는 5대 직무특성들 중 과업 중요성과 자율성에 초점을 맞추어 개인-직무 적합성과 과업 및 맥락 성과의 관계에 대한 이들 두 가지 직무특성들의 조절효과를 고찰하였다. 보다 구체적으로, 본 연구에서는 과업 중요성과 자율성이 개인-직무 적합성과 과업 및 맥락 성과간의 관계에 각각 정(+)의 조절효과를 미칠 것으로 예측하였다. 이러한 연구 가설을 검증하기 위해 층화 표집을 통해 선정된 263개 기업의 종업원 6,044명을 대상으로 설문 조사가 실시되었다. 설문 데이터에 대한 위계적 회귀분석 결과는 개인-직무 적합성과 과업 성과간의 관계는 과업 중요성과 자율성이 높은 경우에 더 강하게 나타난다는 사실을 보여주었다. 그러나, 개인-직무 적합성과 맥락 성과간의 관계에는 과업 중요성만 정의 조절효과를 미치는 것으로 나타났으며, 개인-직무 적합성과 자율성은 맥락 성과와 각각 정의 관계를 보이는 것으로 발견되었다. 본 연구의 결과는 적합성 연구에 대한 이론적 시사점과 직무 배치 및 직무 재설계에 대한 실무적 함의를 갖는다. Person-environment fit theory posits that congruence between individuals’ characteristics and those of their surrounding environments is positively associated with their work outcomes. Among various types of person-environment fit, person-job fit refers to a match between an individual’s characteristics and those of his or her job. While a vast amount of research has investigated the relationships between person-job fit and work outcomes, little attention has been paid to potential moderators between person-job fit and job performance. To fill this gap, we attempted to delineate moderators that can strengthen or weaken the relationship between person-job fit and job performance. Drawing on job characteristics theory, we identified task significance and autonomy as moderators of the linkages between perceived person-job fit and task and contextual performance. Due to the proliferation of a team-based organizational structure and empowerment, the role of task significance and autonomy in work contexts is increasingly important. In the current study, we proposed that the relationships between perceived person-job fit and task and contextual performance would be stronger when task significance and autonomy were high than when they were low. Our hypotheses were tested using large-scale survey data collected from 6,044 employees in 263 Korean companies representing diverse industries, locations, and firm sizes. The results of hierarchical regression analyses supported most of the hypotheses. As predicted, the relationship between perceived person-job fit and task performance was moderated by task significance and autonomy. More specifically, this relationship appeared stronger when task significance and autonomy were higher than when they were low. In addition, the linkage between perceived person-job fit and contextual performance was moderated by task significance. As expected, the association between perceived person-job fit and contextual performance was more pronounced when task significance was high than when it was low. However, we did not detect any moderating effect of autonomy for contextual performance. Instead, we found significant main effects of person-job fit and autonomy on contextual performance. Our findings have several theoretical and practical implications. First of all, the present study contributes to the fit literature by elucidating boundary conditions under which the relationship between person-job fit and task and contextual performance was strengthened. Our results suggest that there are important job characteristics that can intensify the effect of person-job fit, even after controlling individual characteristics such as growth need strength. Based on these findings, managers may need to increase the level of task significance and autonomy to maximize the effect of person-job fit on employee performance. Providing employees with a significant and autonomous job could be an avenue to enhance their task and contextual performance. In conclusion, the current study elaborates on the extant body of knowledge on person-job fit by exploring moderators of the fit-performance relationship using a sample with high external validity.

협응이동훈련이 경도인지장애노인의 낙상물리요인에 미치는 영향

박미희 ( Mihee Park ),이동우 ( Dong-woo Lee ),정모범 ( Mo-beom Jeong ) 대한물리의학회 2020 대한물리의학회지 Vol.15 No.2

PURPOSE: This study examined the effects of coordinative locomotor training on the physical factors for falls in the elderly with mild cognitive impairment. METHODS: This study examined thirty subjects diagnosed with mild cognitive impairment by the radiologic findings, history, and physical examination. The subjects were assigned to a control group (n = 15) or experimental group (n = 15, coordinative locomotor training). The experimental group underwent coordinative locomotor training for four weeks, with training sessions two times per week. The control group was given a fall-prevention education for 60 minutes without coordinative locomotor training. To evaluate the physical factors for falls, the lower extremity strength and the Korean version of the Fullerton advanced balance scale and biorescue were measured for balance. These tests were conducted before and after training. RESULTS: Significant differences were observed between the two groups after the four weeks of coordinative locomotor training for the elderly with mild cognitive impairment the experimental group had a greater degree of improvement in the physical factors for falls. CONCLUSION: These findings suggest that coordinative locomotor training may have a functional effect on fallprevention and the mobility of the elderly with mild cognitive impairment. In addition, it is expected to provide systematic and effective data that can be used as a fall prevention program for the elderly with mild cognitive impairment in each institution.

夏枯草의 간세포에서 항산화 및 미토콘드리아 보호효과

장미희(Mihee Jang),서혜림(Hye Lim Seo),김상찬(Sang Chan Kim),김영우(Young Woo Kim) 한의병리학회 2016 동의생리병리학회지 Vol.30 No.1

Titanium Dioxide/UV Photocatalytic Disinfection in Fresh Carrots

CHO, MIHEE,CHOI, YOONJUNG,PARK, HYOJIN,KIM, KWANSIK,WOO, GUN-JO,PARK, JIYONG ational Association for Food Protection 2007 Journal of food protection Vol.70 No.1

<P>Increased occurrences of fresh produce-related outbreaks of foodborne illness have focused attention on effective washing processes for fruits and vegetables. A titanium dioxide (TiO2) photocatalytic reaction under UV radiation provides a high rate of disinfection. The photo-killing effects of TiO2 on bacteria in liquid cultures under experimental conditions have been widely studied. However, the disinfection effects of the TiO2 photocatalytic reaction on fresh vegetables during a washing process have not been evaluated. Our objectives were to design a pilot-scale TiO2/UV photocatalytic reactor for fresh carrots and to compare the bactericidal effects of the TiO2/UV reaction against bacteria in liquid media and on carrots. TiO2/UV photocatalytic reactions for 40, 60, and 30 s were required for the complete killing of Escherichia coli, Salmonella Typhimurium, and Bacillus cereus (initial counts of approximately 6.7 log CFU/ml), respectively. The counts of total aerobic bacteria in fresh carrots and foodborne pathogenic bacteria in inoculated carrots were also measured. Counts of total aerobic bacteria were reduced by 1.8 log CFU/g after TiO2/UV photocatalytic disinfection for 20 min compared with a 1.1-log CFU/g reduction by UV alone. E. coli, Salmonella Typhimurium, and B. cereus (8 log CFU/ml) were inoculated onto carrots, and the number of surviving bacteria in carrots was determined after treatment. The TiO2/UV treatment exhibited 2.1-, 2.3-, and 1.8-log CFU/g reductions in the counts of E. coli, Salmonella, Typhimurium, and B. cereus, respectively, compared with 1.3-, 1.2-, and 1.2-log CFU/g reductions by UV alone. The TiO2/UV photocatalyst reaction showed significant bactericidal effects, indicating that this process is applicable to nonthermal disinfection of fresh vegetables.</P>

Accuracy of one-step automated orthodontic diagnosis model using a convolutional neural network and lateral cephalogram images with different qualities obtained from nationwide multi-hospitals

Sunjin Yim,Sungchul Kim,Inhwan Kim,Jae-Woo Park,Jin-Hyoung Cho,Mihee Hong,Kyung-Hwa Kang,Minji Kim,Su-Jung Kim,Yoon-Ji Kim,Young Ho Kim,Sung-Hoon Lim,Sang Jin Sung,Namkug Kim,Seung-Hak Baek 대한치과교정학회 2022 대한치과교정학회지 Vol.52 No.1

Objective: The purpose of this study was to investigate the accuracy of one-step automated orthodontic diagnosis of skeletodental discrepancies using a convolutional neural network (CNN) and lateral cephalogram images with different qualities from nationwide multi-hospitals. Methods: Among 2,174 lateral cephalograms, 1,993 cephalograms from two hospitals were used for training and internal test sets and 181 cephalograms from eight other hospitals were used for an external test set. They were divided into three classification groups according to anteroposterior skeletal discrepancies (Class I, II, and III), vertical skeletal discrepancies (normodivergent, hypodivergent, and hyperdivergent patterns), and vertical dental discrepancies (normal overbite, deep bite, and open bite) as a gold standard. Pre-trained DenseNet-169 was used as a CNN classifier model. Diagnostic performance was evaluated by receiver operating characteristic (ROC) analysis, t-stochastic neighbor embedding (t-SNE), and gradientweighted class activation mapping (Grad-CAM). Results: In the ROC analysis, the mean area under the curve and the mean accuracy of all classifications were high with both internal and external test sets (all, > 0.89 and > 0.80). In the t-SNE analysis, our model succeeded in creating good separation between three classification groups. Grad-CAM figures showed differences in the location and size of the focus areas between three classification groups in each diagnosis. Conclusions: Since the accuracy of our model was validated with both internal and external test sets, it shows the possible usefulness of a one-step automated orthodontic diagnosis tool using a CNN model. However, it still needs technical improvement in terms of classifying vertical dental discrepancies.