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W. P. Chen(W. P. Chen),Z. K. Punja(Z. K. Punja) 고려인삼학회 2003 Journal of Ginseng Research Vol.27 No.1
Agrobacterium-mediated transformation of American ginseng (Panax quinquefolius L.) with strain LBA 4404 containing a rice thaumatin-like protein gene is described. The selectable markers used were phosphinothricin acetyltransferase and hygromycin phosphotransferase genes. Epicotyl explants from seedlings were precultured for 5-7 days on Murashige and Skoog medium with α-naphthaleneacetic acid and 2,4 dichlorophenoxyacetic acid at 10 μM and 9 μM, respectively (ND medium), prior to Agrobacterium infection. The explants were immersed in a bacterial suspension for<br/> 20 min. A post-infection co-culture period of 3-4 days was provided on ND medium. Selection for transformed calli was conducted on ND medium with 20 mg/L phosphinothricin followed by 100 mg/L hygromycin over an 8-month period. A transformation frequency of 24.8% was achieved at the callusing phase. The presence of the transgenes in calli was confirmed by Southern hybridization and polymerase chain reaction analysis. The expression of the thaumatin-like protein gene in ginseng calli was demonstrated by Western blot analysis. Somatic embryos were produced from both transgenic calli and suspension cultures, and plantlets were recovered that expressed the transgenic thaumatin-like protein gene.
Yin, Q.Q.,Chang, J.,Zuo, R.Y.,Chen, L.Y.,Chen, Q.X.,Wei, X.Y.,Guan, Q.F.,Sun, J.W.,Zheng, Q.H.,Yang, X.,Ren, G.Z. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2
In order to improve the availability of phytase and probiotics together, a phytase gene from Aspergillus ficuum has been expressed in Lactobacillus. In this study, the transformed Lactobacillus with phytase gene was fed to pigs to determine its effect on pig production, feed conversion and gut microbes. Forty eight, 60-day-old, castrated pigs (Duroc${\times}$Landrace${\times}$Pietrain) were assigned to 6 groups, 8 pigs for each group. Group 1 was the control, group 2 was added with chlortetracycline (500 mg/kg), group 3 was added with the transformed Lactobacillus (500 mg/kg) with 20% (w/w) of calcium monohydrogen phosphate (CMP, $CaHPO_{4}$) removed, group 4 was added with the natural Lactobacillus (500 mg/kg) with 20% (w/w) of CMP removed, group 5 was added with the transformed Lactobacillus (500 mg/kg) with 40% (w/w) of CMP removed, group 6 was added with phytase (500 mg/kg) with 40% (w/w) of CMP removed. The results showed: i) the average daily gain (ADG) was improved in groups 2, 3 and 4 (p<0.05); ii) the diarrhea rates in the groups added with Lactobacillus were lower than in the other groups (p<0.05), in which the transformed Lactobacillus had more effect on reducing digestive disease; iii) the transformed Lactobacillus was most effective in improving the digestibilities of crude protein (CP), calcium (Ca), phosphorus (P), compared with the other groups (p<0.05); iv) Lactobacillus could increase lactic acid bacterium number and ammonia concentrations, and decrease pH values and E. coli number in pig feces (p<0.05); v) the phytase activity in the feces of pigs fed with the transformed Lactobacillus was 133.32 U/g, which was higher than in group 4 (9.58 U/g, p<0.05), and was almost the same as group 6 (135.94 U/g); vi) the transformed Lactobacillus could increase serum concentrations of IgA, triglyceride, and glutamic oxaloacetic transaminase activity (p<0.05), and had no significant effect on other serum indexes (p>0.05).
ZhEng, D. W.,Huang, Y. P.,Tang, T. A.,Cui, Q.,Li, A. Z.,Zhou, S. X.,He, Z. J.,Chen, Z.,Zhang, X. J.,Kwor, R. 대한전자공학회 1993 ICVC : International Conference on VLSI and CAD Vol.3 No.1
A novel process for silicon on insulator(SOI) technology has been presented. Single crystal Si is grown by molecular beam epitaxy(MBF) on a porous silicon(PS) system consisting of two layers of PS with different microstructures. Subsequent lateral oxidation converts the structure to SOI wish excellent insulation property. Si islands with a width of 135㎛ and low doping concentration have been achieved.
Xiao-Yong Zhou,Yan Shen,Er-Tao Hu,Jian-Bo Chen,Yuan Zhao,Ming-Yu Sheng,Jing Li,Yu-Xiang Zheng,Hai-Bin Zhao,Liang-Yao Chen,Wei Li,Xun-Ya Jiang,이영백,David W. Lynch 한국광학회 2013 Current Optics and Photonics Vol.17 No.1
Based on the dispersive feature of the dielectric function of noble metals and the wave vector conservation in physics, both the plasma effect and the complex refractive index, which are profoundly correlated to the complex dielectric function and permeability, have been studied and analyzed. The condition to induce a bulk or a surface plasma in the visible region will not be satisfied, and there will be one solution for the real and the imaginary parts of the refractive index, restricting it only to region I of the complex plane. The results given in this work will aid in understanding the properties of light transmission at the metal/dielectric interface as characterized by the law of refraction in nature.
EXTERNAL COSTS OF INLAND CONTAINER TRANSPORTATION
Paul T-W Lee,Kai-Chieh Hu,Tao Chen,Jo-Ping Hsiung 인하대학교 정석물류통상연구원 2008 인하대학교 정석물류통상연구원 학술대회 Vol.2008 No.3
This paper aims to explore the external costs of inland container transportation in Taiwan, by analyzing the origin and destination of current container cargo in Taiwan, after reviewing an extensive literature survey of methods of external costs. A comparison of external costs between truck and SSS by corridor is made by using a model developed in this paper. Based on the findings that external costs of SSS is considerably lower than truck transport mode and can be an alternative of modal split for current inland container cargo transportation, we discuss the significance and managerial implications of SSS from the perspective of green logistics. In so doing, a top-down approach is employed for developing government policies, which aim to not only reduce the external costs of inland container transportation but also promote SSS in Taiwan.