Lactobacillus (LA-1) and butyrate inhibits osteoarthrits through controling autophagy and inflammatory cell death in chondrocyte

Keun hyung Cho,Hyun-Sik Na,JooYeon Jhun,Jiyoung Kim,Seung Yoon Lee,Jeong soo Lee,In Gyu Um,Seok Jung Kim,Mi-La Cho 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Osteoarthritis (OA) is a disease that reduces quality of life due to pain caused by persistent joint destruction. In addition, as a representative chronic disease, it causes inflammation and affects immunity, and it is one of the diseases that is difficult to cure, so treatment and improvement methods are urgently needed. In a previous study, we published that LA-1 improves osteoarthritis and has cartilage protection by controlling inflammation. However, it was not known how LA-1 improves osteoarthritis in the body. So in this study, it was confirmed that the administration of LA-1 to the MIA-induced OA rat model reduces the pain threshold, protects cartilage, and regulates inflammation markers in the articular synovium. Additionally, collecting and analyzing the feces of the disease model, it affected the gastrointestinal system and improved the environment of the microbiome. Interestingly, by providing LA-1, it was confirmed that the diversity and abundance of microbiome in the intestine were changed, and that the bacteria that produced SCFAs increased. In addition, daily supply of butyrate, one of the SCFAs produced by certain bacteria, triggers autophagy activation and tends to decrease necroptosis. This suggests that systemic immunity as well as OA is regulated according to changes in the intestinal microbial community, and that activation of autophagy can indirectly reduce abnormal cell death. In addition, assuming that osteoarthritis is a chronic degenerative disease, cell analysis was performed using splenocyte and blood assuming that the immune system is deteriorated. As a result, both splenocytes and PBMCs confirmed that regulatory T cells increased and Th17 cells decreased. In summary, providing LA-1 leads to increased production of SCFAs by altering the microbes in the intestine. Accordingly, it is possible to suppress the progression of OA and control pain due to OA, and improve an abnormal joint environment by controlling autophagy and necroptosis.

Morphometric study on the infraorbital foramen in relation to sex and side of the cranium in northeastern Brazil

Laí,s Carolina Santos Cisneiros de Oliveira,Maria Paula Mendonç,a Silveira,Erasmo de Almeida Jú,nior,Francisco Prado Reis,José,Aderval Aragã,o 대한해부학회 2016 Anatomy & Cell Biology Vol.49 No.1

Detailed knowledge of the possible anatomical and morphometric variations of the infraorbital foramen (IOF) is important for ensuring safe and successful regional anesthesia, and for avoiding iatrogenic nerve injuries during surgery on the middle third of the face. To conduct a morphometric study on the IOF, correlating this with sex and side of the cranium. Two hundred forty-two crania were used (148 male and 94 female). Measurements were made with the aid of digital calipers with precision to 0.01 mm. Presence of foramina and their multiplicity was also observed. The data were analyzed descriptively and analytically. Statistical significance was stipulated as 5% (P≤0.05). The IOF was found bilaterally in all the crania, and 26 of them presented multiplicity. The distance from the IOF to the anterior nasal spine was greater in males on both sides (P<0.001). Statistical differences between the sexes were also seen in relation to the following morphometric variables: height of the left IOF (P=0.007), width of the right IOF (P=0.004), and width of the left IOF (P=0.008), and the measurements were also larger among males. The IOF was present in all the crania and on both sides. It was morphometrically larger in males, on both sides.

Autophagic elimination of Trypanosoma cruzi in the presence of metals

Laís Pessanha de Carvalho,Edésio José Tenório de Melo 한국미생물학회 2019 The journal of microbiology Vol.57 No.10

Trypanosoma cruzi is an obligate intracellular parasite transmitted to vertebrate hosts by blood-sucking insects. Molecules present in parasites and mammalian cells allow the recognition and parasite internalization. Metallic ions play an essential role in the establishment and maintenance of hostparasite interaction. However, little is known about how parasites handle with essential and nonessential metal quotas. This study aimed to investigate the influence of metal ions on the biological processes of T. cruzi infected cells. Infected cells were incubated with ZnCl2, CdCl2, and HgCl2 for 12 h and labeled with different specific dyes to investigate the cellular events related to intracellular parasite death and elimination. Infected host cells and parasite’s mitochondria underwent functional and structural disorders, in addition to parasite’s DNA condensation and pH decrease on host cells, which led to parasite death. Further investigations suggested that lysosomes were involved in pH decrease and the double membrane of the endoplasmic reticulum formed vacuoles surrounding damaged parasites, which indicate the occurrence of autophagy for parasite elimination. In conclusion, low concentrations of nonessential and essential metals cause a series of damage to Trypanosoma cruzi organelles, leading to its loss of viability, death, and elimination, with no removal of the host cells.

Cinnamon: an aromatic condiment applicable to chronic kidney disease

Moreira Laís de Souza Gouveia,Brum Isabela de Souza da Costa,de Vargas Reis Drielly C. M.,Trugilho Liana,Chermut Tuany R.,Esgalhado Marta,Cardozo Ludmila F. M. F.,Stenvinkel Peter,Shiels Paul G.,Mafra 대한신장학회 2023 Kidney Research and Clinical Practice Vol.42 No.1

Cinnamon, a member of the Lauraceae family, has been widely used as a spice and traditional herbal medicine for centuries and hasshown beneficial effects in cardiovascular disease, obesity, and diabetes. However, its effectiveness as a therapeutic intervention forchronic kidney disease (CKD) remains unproven. The bioactive compounds within cinnamon, such as cinnamaldehyde, cinnamicacid, and cinnamate, can mitigate oxidative stress, inflammation, hyperglycemia, gut dysbiosis, and dyslipidemia, which are commoncomplications in patients with CKD. In this narrative review, we assess the mechanisms by which cinnamon may alleviate complicationsobserved in CKD and the possible role of this spice as an additional nutritional strategy for this patient group.

Uniform in bandwidth rate of convergence of the conditional mode estimate on functional stationary ergodic data

Naâmane Laïb,Djamal Louani 한국통계학회 2016 Journal of the Korean Statistical Society Vol.45 No.1

The aim of this paper is to establish the uniform consistency with rate over a bandwidth interval of the kernel conditional mode estimate whenever functional stationary ergodic data are considered. This kind of result is immediately applicable to proving uniform consistency of kernel-type estimators when the bandwidth h is a function of the data or the location x. Notice that our uniform in bandwidth results are the first ones to be established in this setting. Moreover, the ergodic setting offers a more general framework in regards to the practice than the usual mixing structure.

복합시술 모발의 자외선 보호처리에 따른 손상도와 색상 변화

라채숙ㆍ권수애ㆍ이옥규(hae Suk LaㆍSoo Ae KweonㆍOk Kyu Lee) 한국인체미용예술학회 2016 한국인체미용예술학회지 Vol.17 No.1

This study aimed to determine the effects of UV-blocking by the type of UV care agent for complex-treated hair. For this, healthy women’s hair was permed, bleached and dyed, and a Polypeptide Protein Treatment and hair sunscreen agent were applied. After that, changes in the degree of wave formation, hair surface conditions, physical properties of the hair and hair hue were examined by irradiating UVB rays. The analysis results were found as follows: In terms of the degree of wave formation, no difference was observed among UV care agents. In terms of hair surface scale, hair damage was lower when the hair was coated twice as opposed to being coated once regardless of the type of the UV care agent and treatment method. In addition, tensile strength, elongation rate and resilience rate were superior in the hair recoated with the hair sunscreen agent. In contrast, no significant difference was observed among the UV care agents in terms of damage rates. Lightness and reflected light were also high in the hair recoated with the hair sunscreen agent. In addition, the a* value, reflection and dominant wavelength were the highest in the hair with post-treatment coating for the skin while b* value, reflection and degree of irritation was the highest in the hair with post-treatment coating for hair. The said results can be summarized as follows: The UV care agent revealed effective UV-blocking effects by maintaining the wave formation and preventing hair damage. When UV rays were irradiated, the hair for post-treatment product was the most efficient in maintaining hair color.

한국작곡가 연구 9 / 나운영 음악자료 6 : 박경호 자료

홍정수(Jung Soo Hong),나운영기념사업회(La,Un-Yung Foundation) 민족음악학회 2001 음악과 민족 Vol.22 No.-

Vector-on-function quantile regression for stationary ergodic processes

Mohamed Chaouch,Naâmane Laïb 한국통계학회 2015 Journal of the Korean Statistical Society Vol.44 No.2

This paper deals with quantile regression of a vector response (Y ∈ Rq, q ≥ 2) on a functional covariate X that takes values in an infinite dimensional space. The main purpose is to introduce a kernel-type estimator of the conditional geometric quantiles whenever functional strictly stationary ergodic data are considered. We established the strong consistency with rate of the proposed estimator as well as the asymptotic distribution that leads to build a confidence region for the multivariate quantile regression. We gave also an application to joint horizon time series forecasting.

Porosity and pore size distribution in high-viscosity and conventional glass ionomer cements: a micro-computed tomography study

Neves Aline Borburema,Lopes Laísa Inara Gracindo,Bergstrom Tamiris Gomes,Silva Aline Saddock Sá da,Lopes Ricardo Tadeu,Neves Aline de Almeida 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.4

Objectives This study aimed to compare and evaluate the porosity and pore size distribution of high-viscosity glass ionomer cements (HVGICs) and conventional glass ionomer cements (GICs) using micro-computed tomography (micro-CT). Materials and Methods Forty cylindrical specimens (n = 10) were produced in standardized molds using HVGICs and conventional GICs (Ketac Molar Easymix, Vitro Molar, MaxxionR, and Riva Self-Cure). The specimens were prepared according to ISO 9917-1 standards, scanned in a high-energy micro-CT device, and reconstructed using specific parameters. After reconstruction, segmentation procedures, and image analysis, total porosity and pore size distribution were obtained for specimens in each group. After checking the normality of the data distribution, the Kruskal-Wallis test followed by the Student-Newman-Keuls test was used to detect differences in porosity among the experimental groups with a 5% significance level. Results Ketac Molar Easymix showed statistically significantly lower total porosity (0.15%) than MaxxionR (0.62%), Riva (0.42%), and Vitro Molar (0.57%). The pore size in all experimental cements was within the small-size range (< 0.01 mm3), but Vitro Molar showed statistically significantly more pores/defects with a larger size (> 0.01 mm3). Conclusions Major differences in porosity and pore size were identified among the evaluated GICs. Among these, the Ketac Molar Easymix HVGIC showed the lowest porosity and void size.

The Role of Bisphosphonates Prior to Denosumab Treatment on Rebound Fractures: A Mini Review

Arthur Costa Inojosa,Laís Mendes,Leonardo Bandeira,Francisco Bandeira 대한골대사학회 2022 대한골대사학회지 Vol.29 No.4

Denosumab is a potent anti-resorptive medication used to treat patients at high risk for osteoporosis; however, its beneficial effects on the skeletal system are quickly reversed after discontinuation. In contrast, bisphosphonates (BPs) are anti-resorptive agents with residual effects on the bone matrix; thus, these are capable of preserving bone mass for a long time. Therefore, subsequent anti-resorptive treatment with BPs is mandatory to prevent rebound fractures. Furthermore, BP administration before denosumab treatment appears to be a reasonable strategy for reducing hyperactivation of bone remodeling. In this review, we summarize the effects of BP administration before denosumab treatment in preventing rebound fractures after denosumab discontinuation.