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Prochnow Fernanda Harumi Oku,Kunz Patricia Valéria Manozzo,Correr Gisele Maria,Kaizer Marina da Rosa,Gonzaga Carla Castiglia 대한치과보존학회 2022 Restorative Dentistry & Endodontics Vol.47 No.4
Objectives This study evaluated the relationship between the battery charge level and irradiance of light-emitting diode (LED) light-curing units (LCUs) and how these variables influence the Vickers hardness number (VHN) of a bulk-fill resin. Materials and Methods Four LCUs were evaluated: Radii Plus (SDI), Radii-cal (SDI), Elipar Deep Cure (Filtek Bulk Fill, 3M Oral Care), and Poly Wireless (Kavo Kerr). Irradiance was measured using a radiometer every ten 20-second activations until the battery was discharged. Disks (4 mm thick) of a bulk-fill resin (Filtek Bulk Fill, 3M Oral Care) were prepared, and the VHN was determined on the top and bottom surfaces when light-cured with the LCUs with battery levels at 100%, 50% and 10%. Data were analyzed by 2-way analysis of variance, the Tukey’s test, and Pearson correlations (α = 5%). Results Elipar Deep Cure and Poly Wireless showed significant differences between the irradiance when the battery was fully charged versus discharged (10% battery level). Significant differences in irradiance were detected among all LCUs, within each battery condition tested. Hardness ratios below 80% were obtained for Radii-cal (10% battery level) and for Poly Wireless (50% and 10% battery levels). The battery level showed moderate and strong, but non-significant, positive correlations with the VHN and irradiance. Conclusions Although the irradiance was different among LCUs, it decreased in half of the devices along with a reduction in battery level. In addition, the composite resin effectiveness of curing, measured by the hardness ratio, was reduced when the LCUs’ battery was discharged. Objectives This study evaluated the relationship between the battery charge level and irradiance of light-emitting diode (LED) light-curing units (LCUs) and how these variables influence the Vickers hardness number (VHN) of a bulk-fill resin. Materials and Methods Four LCUs were evaluated: Radii Plus (SDI), Radii-cal (SDI), Elipar Deep Cure (Filtek Bulk Fill, 3M Oral Care), and Poly Wireless (Kavo Kerr). Irradiance was measured using a radiometer every ten 20-second activations until the battery was discharged. Disks (4 mm thick) of a bulk-fill resin (Filtek Bulk Fill, 3M Oral Care) were prepared, and the VHN was determined on the top and bottom surfaces when light-cured with the LCUs with battery levels at 100%, 50% and 10%. Data were analyzed by 2-way analysis of variance, the Tukey’s test, and Pearson correlations (α = 5%). Results Elipar Deep Cure and Poly Wireless showed significant differences between the irradiance when the battery was fully charged versus discharged (10% battery level). Significant differences in irradiance were detected among all LCUs, within each battery condition tested. Hardness ratios below 80% were obtained for Radii-cal (10% battery level) and for Poly Wireless (50% and 10% battery levels). The battery level showed moderate and strong, but non-significant, positive correlations with the VHN and irradiance. Conclusions Although the irradiance was different among LCUs, it decreased in half of the devices along with a reduction in battery level. In addition, the composite resin effectiveness of curing, measured by the hardness ratio, was reduced when the LCUs’ battery was discharged.
Patricia Giovanella,Lídia de Azevedo Duarte,Daniela Mayumi Kita,Valéria Maia de Oliveira,Lara Durães Sette 한국미생물학회 2021 The journal of microbiology Vol.59 No.7
Soil contamination with diesel oil is quite common duringprocesses of transport and storage. Bioremediation is considereda safe, economical, and environmentally friendly approachfor contaminated soil treatment. In this context, studiesusing hydrocarbon bioremediation have focused on totalpetroleum hydrocarbon (TPH) analysis to assess process effectiveness,while ecotoxicity has been neglected. Thus, thisstudy aimed to select a microbial consortium capable of detoxifyingdiesel oil and apply this consortium to the bioremediationof soil contaminated with this environmental pollutantthrough different bioremediation approaches. Gas chromatography(GC-FID) was used to analyze diesel oil degradation,while ecotoxicological bioassays with the bioindicatorsArtemia sp., Aliivibrio fischeri (Microtox), and Cucumissativus were used to assess detoxification. After 90 days ofbioremediation, we found that the biostimulation and biostimulation/bioaugmentation approaches showed higher ratesof diesel oil degradation in relation to natural attenuation(41.9 and 26.7%, respectively). Phytotoxicity increased in thebiostimulation and biostimulation/bioaugmentation treatmentsduring the degradation process, whereas in the Microtoxtest, the toxicity was the same in these treatments as thatin the natural attenuation treatment. In both the phytotoxicityand Microtox tests, bioaugmentation treatment showed lowertoxicity. However, compared with natural attenuation, thisapproach did not show satisfactory hydrocarbon degradation. Based on the microcosm experiments results, we concludethat a broader analysis of the success of bioremediation requiresthe performance of toxicity bioassays.
Monteiro Aricia Noelli Brega,Petri Bruno Simões Sérgio,Furuya Haroldo,Milanelo Liliane,Scognamillo Márcia Valéria Rizzo,Hayashi Ayne Murata 사단법인약침학회 2022 Journal of Acupuncture & Meridian Studies Vol.15 No.5
Background: The growth of exotic pet medicine is leading to fast developments in clinical investigations on birds. Acupuncture, specifically pharmacopuncture, offers safe chemical restraint options. Objectives: To investigate pharmacopuncture at acupoint GV20 in blue-fronted Amazon parrots (Amazona aestiva) using ketamine and midazolam. Methods: Sixteen healthy birds were distributed into four groups (C: intramuscular control; 1/2 C: 1/2 dose intramuscular control; 1/2 GV20: 1/2 dose at acupoint GV20; 1/5 GV20: 1/5 dose at acupoint GV20). Degree of sedation, latency, recuperation time, heart and respiratory rate, and body temperature were measured. Quantitative data were analyzed by a Student’s t-test. Results: The C, 1/2 C, and 1/2 GV20 groups showed the same degree of sedation. The 1/2 GV20 group showed longer latency times (6 ± 2.1) than the 1/2 C (2.5 ± 0.5) group. Sedation time did not differ between the C (28 ± 9.8), 1/2 C (30.5 ± 8.6), and 1/2 GV20 (41 ± 22.24) groups. The 1/2 GV20 group recuperated faster (13.7 ± 3.7) than the C group (64.2 ± 3.5). The C and 1/2 C groups showed tremors and slow and unstable recovery. Two animals in the C group showed mild hypothermia (38°C). Conclusion: The use of 1/2 GV20 was effective and safe to sedate blue-fronted Amazon parrots without side effects, providing easy, stable, and fast recovery. The use of 1/5 GV20 had a shorter sedation time. These findings show that the combination of acupuncture and drugs provides new possibilities for efficient anesthetic protocols with fewer side effects in birds.
Luiz Paulo Carvalho Rocha,Tânia de Carvalho Rocha,Stephanie de Cássia Carvalho Rocha,Patrícia Valéria Henrique,Flávio Ricardo Manzi,Micena Roberta Miranda Alves e Silva 대한영상치의학회 2020 Imaging Science in Dentistry Vol.50 No.2
Purpose: In this study, we used ultrasonography to monitor the use of hyaluronic acid (HA) as a filler in the face for esthetic reasons. We monitored changes in the filler shape, distribution, and relationship with adjacent anatomical structures over a 180-day period. Materials and Methods: Two patients each received an ultrasound-guided injection of HA, with different products and application sites for each patient. In 1 patient, the injection was administered in the angle of the mandible, while in the other, it was administered in the zygomatic region. The injection sites were monitored via ultrasonography at 24 hours, 30 days, and 180 days, at which times the imaging characteristics of the filler were observed. All injections were performed by the same professional, as were the ultrasound exams, which were conducted using the same equipment. Results: In both cases, the HA fillers were visualized using ultrasound at all time points. Some differences were observed between the cases in the images and the distribution of the pockets of filler. In 1 case, the filler appeared as a dark hypoechoic region with well-defined contours, and the material was observed to have moved posteriorly by the 180-day mark. In the other case, the material appeared hyperechoic relative to the previous case and presented no noticeable changes in its anteroposterior distribution over time. Conclusion: Based on these 2 cases, ultrasonography can be a complementary tool used to monitor facial fillers over the long term, allowing for the dynamic observation of different fillers.
Cassamo U. Mussagy,Ariane A. Oshiro,Caio A. Lima,Camila F. Amantino,Fernando L. Primo,Valéria C. Santos-Ebinuma,Rondinelli D. Herculano 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.119 No.-
Synthetic-based colorants have been extensively used in several industries, viz., cosmeceutical, feed,pharmaceuticals, food and textile. However, the growing awareness of potential teratogenicity, carcinogenicityand environmental concerns of certain synthetic colorants have expressed several limitationsregarding their use in some products. These limitations, justifies the motivation to look for ecofriendlyalternatives (cf., natural colorants) to be used as coloring agents for industrial applications. The filamentous fungi Talaromyces amestolkiae appeared as a promising source of natural fluorescentred colorant (NFRC) for the development of custom-made latex gloves. In this study, some basic colorantproperties, including 3D-fluorescence and CIELAB color characteristics were first investigated systematicallybefore their incorporation in natural rubber latex (NRL) films. The NFRC-NRL films properties wereevaluated by physicochemical tests and toxicity through cell viability using 3T3 fibroblasts, wherein eluateof NFRC-NRL films (2.28 AU) revealed viability >70 % (exposing the biocompatibility). Regardless ofthe strategy proposed, it is shown as a proof-of-concept the potential application of NFRC in the NRLmatrix as a biocompatible alternative to produce sustainable commercial colored custom-made latexgloves.
α-Galactosidases Production by Debaryomyces hansenii UFV-1
Pollyanna Amaral Viana,Sebastião Tavares de Rezende,Flávia Maria Lopes Passos,Solimar Gonçalves Machado,Gabriela Picollo Maitan,Vinicio Tadeu da Silva Coelho,Valéria Monteze Guimarães 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.3
Extracellular and intracellular α-galactosidases were produced by yeast Debaryomyces hansenii UFV-1grown on different media with several carbon sources. D. hansenii grown in YP-medium (1% yeast extract and 2%peptone) presented maximum cell mass (8.45 mg/mL) after 36 h of cultivation, with lactose as carbon source, followed by sucrose, glucose, raffinose, and galactose. Higher extracellular and intracellular α-galactosidases activities were observed at 48 h of D. hansenii cultivation in YPmedium containing galactose (0.97 and 5.27 U/mL) and lactose (1.28 and 4.88 U/mL), supporting the evidence for the model of induction for the yeast GAL/MEL regulon,such as described in Sacharomyces cereviseae.