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α-Galactosidases Production by Debaryomyces hansenii UFV-1
Pollyanna Amaral Viana,Sebastião Tavares de Rezende,Flávia Maria Lopes Passos,Solimar Gonçalves Machado,Gabriela Picollo Maitan,Vinicio Tadeu da Silva Coelho,Valéria Monteze Guimarães 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.3
Extracellular and intracellular α-galactosidases were produced by yeast Debaryomyces hansenii UFV-1grown on different media with several carbon sources. D. hansenii grown in YP-medium (1% yeast extract and 2%peptone) presented maximum cell mass (8.45 mg/mL) after 36 h of cultivation, with lactose as carbon source, followed by sucrose, glucose, raffinose, and galactose. Higher extracellular and intracellular α-galactosidases activities were observed at 48 h of D. hansenii cultivation in YPmedium containing galactose (0.97 and 5.27 U/mL) and lactose (1.28 and 4.88 U/mL), supporting the evidence for the model of induction for the yeast GAL/MEL regulon,such as described in Sacharomyces cereviseae.