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RISS 인기검색어
- 검색키워드
- 저자 : V. Suresh Babu (검색결과 13 건)
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Suresh Babu, C. V.,Cho, Sung Gook,Sook Yoo, Young WILEY-VCH Verlag 2005 Electrophoresis Vol.26 No.19
<P>Signal transduction studies have indicated that Akt is essential for transducing the signals originating from extracellular stimuli. An exploration of the Akt signal transduction mechanism depends on the ability to assay its activation states by determining the ability of Akt to phosphorylate various substrates. This paper describes a CE-based kinase assay for Akt using a UV detection method. The RPRAATF peptide was used as the specific substrate to determine the Akt activity. Under the CE separation conditions used, the phosphorylated and nonphosphorylated forms of the RPRAATF peptide were rapidly resolved in the Akt reaction mixture within 20 min. Using this method for measuring the Akt activity, the incubation time for the Akt reactions as well as the kinetic parameters (K<SUB>M</SUB>) were examined. Furthermore, the developed method was applied to a PC12 cell system to assess the dynamics of the Akt activity by examining the effectiveness of the RPRAATF peptide substrate under various cytokine-stimulated environments. These results highlight the feasibility of the CE method, which is a simple and reliable technique for determining and characterizing various enzyme reactions particularly kinase enzymes.</P>
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Babu, C.V. Suresh,Chung, Bong Chul,Lho, Dong Seok,Yoo, Young Sook Elsevier 2006 Journal of chromatography Vol.1111 No.2
<P><B>Abstract</B></P><P>Immunoassays are commonly used in bioresearch for the detection and quantification of small proteins and macromolecules in biological fluids and other complex matrices. In this report, a competitive immunoassay using capillary electrophoresis (CE) with laser-induced fluorescence was developed for methionine-enkephalin (ME). The method is based on the competitive reaction between the ME and fluorescein conjugated ME (ME-F) with anti-ME antibody, capillary electrophoresis separation of the ME-antibody bound and free ME-F, followed by the laser-induced fluorescence detection of the fluorescent species. With the optimized separation conditions, it was possible to separate the antibody bound and free fluorescien conjugated ME by a capillary electrophoresis–laser-induced fluorescence (CE–LIF) analysis using an uncoated fused-silica capillaries. The results concluded that the assay specificity, selectivity and accuracy were excellent.</P>
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Capillary electrophoresis at the omics level: Towards systems biology
Babu C. V., Suresh,Song, Eun Joo,Babar, Sheikh Md. Enayetul,Wi, Mun Hyung,Yoo, Young Sook WILEY-VCH Verlag 2006 Electrophoresis Vol.27 No.1
<P>Emerging systems biology aims at integrating the enormous amount of existing omics data in order to better understand their functional relationships at a whole systems level. These huge datasets can be obtained through advances in high-throughput, sensitive, precise, and accurate analytical instrumentation and technological innovation. Separation sciences play an important role in revealing biological processes at various omic levels. From the perspective of systems biology, CE is a strong candidate for high-throughput, sensitive data generation which is capable of tackling the challenges in acquiring qualitative and quantitative knowledge through a system-level study. This review focuses on the applicability of CE to systems-based analytical data at the genomic, transcriptomic, proteomic, and metabolomic levels.</P>
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A Synchrophasor-Based Line Protection for Single Phase-Ground Faults
Babu N. V. Phanendra,Babu P. Suresh,Roy Saptarshi,Babu T. Sudhakar,Bharadwaj Anil 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.3
Synchrophasor measurement data enhances the transmission line protection. This paper proposes an improved line protection against single phase-ground faults using synchronized phasor data. This algorithm prevents the relay mal-operation caused by high fault resistance. This algorithm calculates the phase difference between relay point voltage and fault point voltage based on the relation between negative sequence of relay point current and fault point current. After, the calculated phase difference between relay point voltage and fault point voltage will be compared with set point voltage phase referred from the relay point voltage phase. The fault detection action will be taken according to a certain phase difference relation between fault point voltage and set point voltage. This method is then applied to a practical single machine single line system. The results show that the suggested algorithm could determine in-line faults accurately with less computational time. It also has proved that this method is immune to the fault resistance, system conditions.
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Latha, V.L. Padma,Reddy, N. Sudhakar,Babu, A. Suresh International Journal of Computer ScienceNetwork S 2021 International journal of computer science and netw Vol.21 No.12
Now that we're in the big data era, data has taken on a new significance as the storage capacity has exploded from trillion bytes to petabytes at breakneck pace. As the use of cloud computing expands and becomes more commonly accepted, several businesses and institutions are opting to store their requests and data there. Cloud storage's concept of a nearly infinite storage resource pool makes data storage and access scalable and readily available. The majority of them, on the other hand, favour a single cloud because of the simplicity and inexpensive storage costs it offers in the near run. Cloud-based data storage, on the other hand, has concerns such as vendor lock-in, privacy leakage and unavailability. With geographically dispersed cloud storage providers, multicloud storage can alleviate these dangers. One of the key challenges in this storage system is to arrange user data in a cost-effective and high-availability manner. A multicloud storage architecture is given in this study. Next, a multi-objective optimization problem is defined to minimise total costs and maximise data availability at the same time, which can be solved using a technique based on the non-dominated sorting genetic algorithm II (NSGA-II) and obtain a set of non-dominated solutions known as the Pareto-optimal set.. When consumers can't pick from the Pareto-optimal set directly, a method based on Principal Component Analysis (PCA) is presented to find the best answer. To sum it all up, thorough tests based on a variety of real-world cloud storage scenarios have proven that the proposed method performs as expected.
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R. Resmi,V. Suresh Babu,M. R. Baiju 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.6
Micro/nanobeam-based resonators have found extensive applications in the micro/nanoelectromechanical system industry. Thermoelastic damping (TED) is a major energy loss issue in micro/nanobeam resonators that limits their important performance parameter, namely, the TED limited quality factor (Q TED ). The critical length (L c ) of a micro/nanobeam is another significant parameter that accounts for the maximum peak in the energy dissipation curve at which Q TED assumes a minimum value. To evaluate Q TED and Lc explicitly when the size of devices is scaled down, size effects play a decisive role and classical theories are inadequate. In this work, a higher-order theory, namely, modified couple stress theory (MCST), is used to overcome the size effects by including one internal material length scale parameter (l). The material-dependent thermoelastic coupled equations for a deflected Euler-Bernoulli microbeam are presented using variational and Hamilton principles. Moreover, the solutions for Q TED are developed on the basis of a complex frequency approach with the appropriate material indices. The effects of material length scale parameters, material performance indices, mechanical boundary conditions (clamped-clamped, simply supported, and cantilever types), mode switching, and plane stress/strain conditions on Q TED and Lc are analyzed. Numerical results are extracted from the analytical expressions by using MATLAB R2015a to quantify thermoelastic energy dissipation. The numerically computed Q TED and L c values are fully investigated to design high-performance resonators. The analyses verify that Q TED is enhanced by optimizing the structural material and augmenting the material length scale parameter. The material order in which Q TED is enhanced is the same for classical theories and MCST, i.e., it is inversely related to the TED index parameter. The influences of boundary types and mode switching on Q TED are relatively less in accordance with the analysis. The effect of plane stress condition compared with that of plane strain condition on Q TED is also remarkable. The L c of the beam is determined to be dependent on the thermal diffusion length of the material used. From an adequate material point of view, poly-silicon has been proven to provide the maximum quality factor while silicon carbide yields the maximum L c . These observations are significant and extremely helpful when designing low-loss micro/nanobeam resonators with superior performance by suitably selecting their geometry and structural materials.
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User Behaviour Profiling in Cloud using One Class SVM : A Review
Vijaya Lakshmi Paruchuri,S. Suresh Babu,P S V S Sridhar,Debnath Bhattacharyya,Hye-Jin Kim 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.9
Distributed computing guarantees to on a very basic level change the way we utilize PCs and get to and store our own specific and business data. With these new registering and correspondences models develop new data security challenges. Existing information security structures, for instance, encryption have fizzled in imagining information theft strikes, particularly those executed by an insider to the cloud supplier. We propose a substitute methodology for securing information in the cloud utilizing adversarial mimic improvement. We screen data access in the cloud and perceive unpredictable data access outlines. Right when unapproved access is suspected and after that confirmed using test questions, we dispatch a disinformation strike by giving back a considerable measure of fake information to the attacker. This secures against the misuse of the customer's real data. Trials coordinated in a neighbor-hood archive setting give confirmation this technique may give unprecedented levels of customer data security in a Cloud space.
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A Study on Performance of 3D RC Frames with Masonry in-fill under Dynamic Loading Conditions
R. Srinivasan,S. Suresh Babu,Sidramappa V. Itti 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.1
This paper enlightens the response or behavior of 3D RC frames with various configurations & amount of masonry in-fill, under dynamic loading conditions. A 1:3 scale, two bay three storey models of a typical office building were constructed with various locations and percentage of masonry in-fill. Components of structure ie, structural sub-assemblages of columns, beams and slabs were casted from the same materials. A series of various ground motion tests were performed on the building model using servo controlled hydraulic actuator. The dynamic characteristics, such as frequency, acceleration and amplitude were identified at each frequency level. The effect of de-bonding of masonry in-fill with RC frames with increase in base acceleration was also observed in this study. Analytical models were developed to predict the natural characteristics and dynamic properties of structure. In modal analysis, masonry in-fill was modeled as equivalent diagonal strut and thickness of strut was taken as equal to in-fill thickness. Material properties and boundary conditions were assigned suitably for in-fill.
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