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Li Tuo,Xiao-Rui Yan 한국미생물학회 2019 The journal of microbiology Vol.57 No.8
A Gram-negative, aerobic, short-rod-shaped, motile (with a terminal flagellum), non-spore-forming bacterium, designated strain 85T, was isolated from a surface-sterilized bark of Sonneratia caseolaris collected from Qinzhou in Guangxi, China and was analyzed using a polyphasic approach to determine its taxonomic position. Strain 85T grew optimally in the presence of 1–2% (w/v) NaCl at 30°C and pH 6.0–7.0. Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 85T belonged to the genus Fulvimarina and shared the highest 16S rRNA gene sequence similarity with Fulvimarina pelagi HTCC2506T (96.16%). The cell-wall peptidoglycan contained meso-diaminopimelic acid and ubiquinone Q-10 was the predominant respiratory lipoquinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified amino lipid, three unidentified phospholipids and six unidentified lipids. The major fatty acid was C18:1 ω7c. The DNA G+C content of strain 85T was 65.4 mol%, and the average nucleotide identity and estimated DDH values between strain 85T and the type strain of Fulvimarina pelagi HTCC2506T were 77.3% and 21.7%, respectively. Based on the phylogenetic, phenotypic, and chemotaxonomic analyses, strain 85T should be considered as a novel species of the genus Fulvimarina with the proposed name Fulvimarina endophytica sp. nov., and its type strain is 85T (= KCTC 62717T = CGMCC 1.13665T).
Li, J.L.,Li, N.,Lee, H.S.,Xing, S.S.,Qi, S.Z.,Tuo, Z.D.,Zhang, L.,Li, B.B.,Chen, J.G.,Cui, L. Elsevier 2016 Fitoterapia Vol.109 No.-
<P>Four new sesqui-lignans, (7R, 7'R, 7 '' S, 8S, 8'S, 8 '' S)-4',5 ''-dihydroxy-3,5,3',4 ''-tetramethoxy-7,9':7',9-diepoxy4,8 ''-oxy-8,8'-sesquineo-lignan-7',9 ''-diol (1), (7R, 7'R, 7 '' S, 8S, 8'S, 8 '' S)-4',3'-dihydroxy-3,5,3',5',4'pentamethoxy-7,9':7',9-diepoxy-4,8'-oxy-8,8'-sesquineo-lignan-7',9'-diol (2), (7R, 7'R, 7'S, 8S, 8'S, 8'S)-3',4'dihydroxy-3,5,4',5 ''-tetramethoxy-7,9':7',9-diepoxy-4,8'-oxy-8,8'-sesquineo-lignan-7',9'-diol (3) and acanthopanax A (7) together with three known compounds (4-6) were isolated from the EtOAc-soluble extract of Acanthopanax senticosus. Their structures were elucidated on the basis of spectroscopic and physicochemical analyses. All the isolates were evaluated for in vitro inhibitory activity against DGAT1 and DGAT2. Among them, compounds 1-6 were found to exhibit selective inhibitory activity on DGAT1 with IC50 values ranging from 61.1 1.3 to 97.7 1.1 111\4 and compound 7 showed selective inhibition of DGAT2 with IC50 value 93.2 1.2. (C) 2016 Elsevier B.V. All rights reserved.</P>
Three Distinct Deformation Behaviors of Cementite Lamellae in a Cold-Drawn Pearlitic Wire
Tuo Xin,Guiju Liu,Wenshuang Liang,Rongsheng Cai,Honglei Feng,Chen Li,Jian Li,Yiqian Wang 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.4
High-resolution transmission electron microscopy is used to investigate the deformation behaviors of cementite lamellaein the heavily cold-drawn piano wires. Three distinct morphologies of cementite are observed, namely, complete lamella,partly-broken lamella and nearly-disappeared lamella. For the complete cementite lamella, it remains a single-crystallinestructure. For the partly-broken cementite lamella, polycrystalline structure and neck-down region appear to release theresidual strain. The lattice expansion of ferrite takes place in two perpendicular directions indicating that the carbon atomsdissolve from cementite into ferrite lattices. An orientation relationship is found between ferrite and cementite phases in thecold-drawn pearlitic wire.
BanBan Li,Jia Lin Li,Na Li,Shi-Zhou Qi,이현선,Le Zhang,Shan-Shan Xing,Zhen Dong Tuo,Long Cui 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.11
Two new furofuran lignans were isolated fromthe stems of Acanthopanax senticosus, along with sevenknown compounds. Their structures were all determined byspectroscopic analyses and chemical methods. All theisolates were evaluated for in vitro inhibitory activityagainst DGAT1 and DGAT2. Compounds 1 and 2 werefound to exhibit selective inhibitory activity on DGAT1with IC50 values 89.5 ± 1.5 and 57.5 ± 1.3 lM,respectively.
Isolation and Characterization of Cold- Adapted PGPB and Their Effect on Plant Growth Promotion
( Mingyuan Li ),( Jilian Wang ),( Tuo Yao ),( Zhenlong Wang ),( Huirong Zhang ),( Changning Li ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.9
Cold-adapted plant growth-promoting bacteria (PGPB) with multiple functions are an important resource for microbial fertilizers with low-temperature application. In this study, culturable coldadapted PGPB strains with nitrogen fixation and phosphorus solubilization abilities were isolated. They were screened from root and rhizosphere of four dominant grass species in nondegraded alpine grasslands of the Qilian Mountains, China. Their other growth-promoting characteristics, including secretion of indole-3-acetic acid (IAA), production of siderophores and ACC deaminase, and antifungal activity, were further studied by qualitative and quantitative methods. In addition, whether the PGPB strains could still exert plant growth-promoting activity at 4°C was verified. The results showed that 67 isolates could maintain one or more growth-promoting traits at 4°C, and these isolates were defined as cold-adapted PGPB. They were divided into 8 genera by 16S rRNA gene sequencing and phylogenetic analysis, of which Pseudomonas (64.2%) and Serratia (13.4%) were the common dominant genera, and a few specific genera varied among the plant species. A test-tube culture showed that inoculation of Elymus nutans seedlings with cold-adapted PGPB possessing different functional characteristics had a significant growth-promoting effect under controlled low-temperature conditions, including the development of the roots and aboveground parts. Pearson correlation analysis revealed that different growth-promoting characteristics made different contributions to the development of the roots and aboveground parts. These cold-adapted PGPB can be used as excellent strain resources suitable for the near-natural restoration of degraded alpine grasslands or agriculture stock production in cold areas.
Li Xiang,Peng Zhiming,Long Lingli,Lu Xiaofang,Zhu Kai,Tuo Ying,Chen Ningning,Zhao Xiaoyang,Wang Le,Wan Yong 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-
Traditional therapeutic strategies for spinal cord injury (SCI) are insufficient to repair locomotor function because of the failure of axonal reconnection and neuronal regeneration in the injured central nervous system (CNS). Neural stem cell (NSC) transplantation has been considered a potential strategy and is generally feasible for repairing the neural circuit after SCI; however, the most formidable problem is that the neuronal differentiation rate of NSCs is quite limited. Therefore, it is essential to induce the neuronal differentiation of NSCs and improve the differentiation rate of NSCs in spinal cord repair. Our results demonstrate that both Wnt5a and miRNA200b-3p could promote NSC differentiation into neurons and that Wnt5a upregulated miRNA200b-3p expression through MAPK/JNK signaling to promote NSC differentiation into neurons. Wnt5a could reduce RhoA expression by upregulating miRNA200b-3p expression to inhibit activation of the RhoA/Rock signaling pathway, which has been reported to suppress neuronal differentiation. Overexpression of RhoA abolished the neurogenic capacity of Wnt5a and miRNA200b-3p. In vivo, miRNA200b-3p was critical for Wnt5a-induced NSC differentiation into neurons to promote motor functional and histological recovery after SCI by suppressing RhoA/Rock signaling. These findings provide more insight into SCI and help with the identification of novel treatment strategies.
Research on Fitness Function of Two Evolution Algorithms Used for Neutron Spectrum Unfolding
Li Rui,Yang Jianbo,Tuo Xianguo,Shi Rui 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.78 No.2
When evolution algorithms are used to unfold the neutron energy spectrum, fitness function design is an important fundamental work for evaluating the quality of the solution, but it has not attracted much attention. In this work, we investigated the performance of eight fitness functions attached to the genetic algorithm (GA) and the differential evolution algorithm (DEA) used for unfolding four neutron spectra selected from the IAEA 403 report. Experiments show that the fitness functions with a maximum in the GA can limit the ability of the population to percept the fitness change, but the ability can be made up in the DEA. The fitness function with a feature penalty term helps to improve the performance of solutions, and the fitness function using the standard deviation and the Chi-squared result shows the balance between the algorithm and the spectra. The results also show that the DEA has good potential for neutron energy spectrum unfolding. The purposes of this work are to provide evidence for structuring and modifying the fitness functions and to suggest some genetic operations that should receive attention when using the fitness function to unfold neutron spectra.