Endocrine Disrupting Organotin Compounds are Potent Inducers of Imposex in Gastropods and Adipogenesis in Vertebrates

Iguchi, Taisen,Katsu, Yoshinao,Horiguchi, Toshihiro,Watanabe, Hajime,Blumberg, Bruce,Ohta, Yasuhiko The Korean Society of Toxicogenomics and Toxicopro 2007 Molecular & cellular toxicology Vol.3 No.1

The persistent and ubiquitous environmental contaminant, tributyltin chloride (TBT), induces not only imposex in gastropods but also the differentiation of adipocytes in vitro and increases adipose mass in vivo in vertebrates. TBT is a nanomolar affinity ligand for retinoid X receptor (RXR) in the rock shell(Thais clavigera) and for both the RXR and the peroxisome proliferator activated receptor $\gamma(PPAR\gamma)$ in the amphibian (Xenopus laevis), mouse, and human. The molecular mechanisms underlying induction of imposex by TBT have not been clarified, though several hypotheses are proposed. TBT promotes adipogenesis in the murine 3T3-L1 cell model and perturbs key regulators of adipogenesis and lipogenic pathways in vivo primarily through activation of RXR and $PPAR\gamma$. Moreover, in utero exposure to TBT leads to strikingly elevated lipid accumulation in adipose depots, liver, and testis of neonate mice and results in increased adipose mass in adults. In X. laevis, ectopic adipocytes form in and around gonadal tissues following organotin, RXR or $PPAR\gamma$ ligand exposure. TBT represents the first example of an environmental endocrine disrupter that promotes adverse effects from gastropods to mammals.

한국 남서해 연안역의 유기주석합물의 오염에 관한 연구

조현서,굴구민굉 (堀口敏宏) ( Hyeon Seo Cho,Toshihiro Horiguchi ) 한국물환경학회 1997 학술연구발표회 논문초록집 Vol.1997 No.1

광양만과 여수해만의 표층퇴적물에서 Nonylphenol의 오염에 관한 연구

조현서,김용옥,설순우,-- 한국환경과학회 2004 한국환경과학회지 Vol.13 No.6

This study was carried out to survey the pollution of nonvlphenol (NP) in surface sediments around Gwangyang bay and Yeosu sound. NP was suspected chemicals as endocrine disruption. Gwangyang bay is located on the mid south coast of Korea. It is a semi-closed bay which Yeosu petrochemical industrial complex, POSCO (Pohang Steel Company) and Gwangyang container harbor are there. The surface sediments were collected at 15 stations with gravity corer at October, 1999, February, May and August, 2000. Also, the stream and intertidal sediment were collected at 5 sites at August, 2000. Concentrations of NP in surface sediments were in the range of 6.89 to 202.70 ng/g dry wt.. Seasonal range (mean value) of NP is 13.98 to 30.48 (23.46) ng/g dry wt. at October, 10.35 to 54.91 (28.10) ng/g dry wt. at February, 29.05 to 202.70 (82.32) ng/g dry wt. at May and 6.98 to 83.40 (25.37) ng/g dry wt. at August. NP was seasonally fluctuated, and the highest mean value and range was detected at May, 2000. NP was highly distributed in the inner part of Gwangyang bay than Yeosu sound. Concentrations of NP in stream and intertidal sediments showed the highest value in downstream near Yeosu petrochemical industrial complex and Yondung stream. It suggests that the source of NP is industrial wastewater and municipal sewage.

Immune gene mining by pyrosequencing in the rockshell, <i>Thais clavigera</i>

Rhee, Jae-Sung,Kim, Bo-Mi,Jeong, Chang-Bum,Horiguchi, Toshihiro,Lee, Young-Mi,Kim, Il-Chan,Lee, Jae-Seong Elsevier 2012 FISH AND SHELLFISH IMMUNOLOGY Vol.32 No.5

<P><B>Abstract</B></P><P>The rockshell, <I>Thais clavigera</I> (Gastropoda: Muricidae) has been shown to be a useful species as a potential indicator for diverse pollution in the marine environment. However, their genetic information is still not widely available. Here, we performed an extensive transcriptome analysis of <I>T. clavigera</I> using the pyrosequencing method, and selected innate immune-related genes. Among the unigenes obtained in this species, we annotated a number of immune system-related genes (e.g. adhesive protein, antimicrobial protein, apoptosis- and cell cycle-related protein, cellular defense effector, immune regulator, pattern recognition protein, protease, protease inhibitor, reduction/oxidation-related protein, signal transduction-related protein and stress protein), which are potentially useful for immunity research in this species. To confirm the usefulness of potential immune-biomarker genes, we checked the transcript level of specific immune genes in both different tissues and LPS-exposed rockshells within the <I>T. clavigera</I> transcript database. This study would be helpful to extend our knowledge on the immune system of rockshell in comparative aspects. Also it would be useful to develop the rockshell as a potential test organism for monitoring of marine environment quality.</P> <P><B>Highlights</B></P><P>► Novel investigation of extensive immune-relevant genes in the rockshell, <I>Thais clavigera</I> as the first report. ► Confirm the usefulness for comparative analysis of specific immune gene in different developmental tissues of rockshell. ► Development of potential immune-biomarker gene upon bacterial challenge. ► Contribution on immune-relevant gene information resource for comparative gene expression analysis within aquatic invertebrates.</P>

광양만 주변해역의 표층퇴적물 중 Bisphenol A의 오염에 관한 연구

조현서(Hyeon-Seo Cho),김용옥(Yong-Ok Kim),신태선(Tai-Sun Shin),Toshihiro Horiguchi 한국해양환경·에너지학회 2004 한국해양환경·에너지학회지 Vol.7 No.2

Effect of water temperature on survival of early-life stages of marbled flounder <i>Pseudopleuronectes yokohamae</i> in Tokyo Bay, Japan

Lee, Jeong-Hoon,Kodama, Keita,Oyama, Masaaki,Shiraishi, Hiroaki,Horiguchi, Toshihiro Elsevier 2017 Marine environmental research Vol.128 No.-

<P><B>Abstract</B></P> <P>We investigated factors that might have disturbed the stock recovery of marbled flounder in Tokyo Bay by focusing on the early life stages. Field surveys in Tokyo Bay from 2006 to 2011 revealed that mature adult biomass increased from 2006 to 2008 and decreased thereafter. Meanwhile, larval and juvenile densities were high in 2006 and 2008 but low in other years. Discrepancies in the yearly trends of these parameters suggest that mortality during life stages between spawning and early larval phases might have affected the abundance of the subsequent life stages. Monthly mean water temperature between January and February, in which hatching and pelagic larvae occur in the bay, was lower in 2006 (8.6 °C) and 2008 (9.6 °C) than was observed in other years (10.4–11.4 °C). Significant negative correlation between water temperature and larval density implies that mortality during pre- and post-larval stages would be higher in warmer winter years (>10 °C). To test this hypothesis, we examined the effects of water temperature on mortality and development in egg and larval stages under controlled laboratory conditions. Hatching rate was high in a water temperature range of 9.2–12.7 °C (66.6–82.5%), whereas it decreased in cooler (3.7% at 5.9 °C) or warmer (33.9% at 14.8 °C) conditions. Meanwhile, days from fertilization to hatching, size of larvae at hatching and survival rate of larvae after 18 d from hatching were monotonically and significantly decreased as water temperature was elevated. Combined evidence of the field and laboratory studies suggests that a warmer reproductive season (>10 °C) might induce mortalities of marbled flounder larvae in Tokyo Bay.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effects of temperature on the early life stages of marbled flounder were examined. </LI> <LI> Larval and juvenile densities in Tokyo Bay were low during warmer winter years >10 °C. </LI> <LI> High larval mortality in warmer conditions was confirmed by laboratory experiments. </LI> <LI> Elevated temperature during reproductive season may result in recruitment failure. </LI> </UL> </P>

Transfer kinetics of perfluorooctane sulfonate from water and sediment to a marine benthic fish, the marbled flounder ( <i>Pseudopleuronectes yokohamae</i> )

Sakurai, Takeo,Kobayashi, Jun,Kinoshita, Kyoko,Ito, Nozomi,Serizawa, Shigeko,Shiraishi, Hiroaki,Lee, Jeong-Hoon,Horiguchi, Toshihiro,Maki, Hideaki,Mizukawa, Kaoruko,Imaizumi, Yoshitaka,Kawai, Toru,Suz Wiley Periodicals 2013 Environmental toxicology and chemistry Vol.32 No.9

<P>The authors investigated the kinetics of transfer of perfluorooctane sulfonate (PFOS) from water, suspended sediment, and bottom sediment to a marine benthic fish, the marbled flounder (<I>Pseudopleuronectes yokohamae</I>). Fish were exposed in 3 treatments to PFOS in combinations of these exposure media for 28 d and then depurated for 84 d. A major part (37–66%) of PFOS in the fish was in the carcass (i.e., whole body minus muscle and internal organs). Three first-order-kinetic models that differed in exposure media, that is, 1) sum of dissolved and particulate phases and sediment; 2) dissolved phase, particulate phase, and sediment; and 3) dissolved phase only, were fitted to the data assuming common rate constants among the treatments. The uptake efficiency of dissolved PFOS at the respiratory surfaces was estimated to be 3.2% that of oxygen, and the half-life of PFOS in the whole body to be 29 d to 31 d. The better fit of models 1 and 2 and the values of the estimated uptake rate constants suggested that the PFOS in suspended and bottom sediments, in addition to that dissolved in water, contributed to the observed body burden of the fish. Based on an evaluation of several possible contributing factors to the uptake of PFOS from suspended and bottom sediments, the authors propose that further investigation is necessary regarding the mechanisms responsible for the uptake. <I>Environ Toxicol Chem</I> 2013;32:2009–2017. © 2013 The Authors. <I>Environmental Toxicology and Chemistry</I> Published by Wiley Periodicals, Inc., on behalf of SETAC. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.</P>