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Nguyen, Khanh Hoang Viet,Dao, Trong Khoa,Nguyen, Hong Duong,Nguyen, Khanh Hai,Nguyen, Thi Quy,Nguyen, Thuy Tien,Nguyen, Thi Mai Phuong,Truong, Nam Hai,Do, Thi Huyen Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.5
Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.
Social Capital and Migration: A Case Study of Rural Vietnam
Hong Thu NGUYEN,My Kim LE,Thi Thuy Dung NGUYEN,Vu Phuong Linh DAO,Ngoc Tien NGUYEN 한국유통과학회 2022 The Journal of Asian Finance, Economics and Busine Vol.9 No.1
To investigate the short-run effects of social capital on migration decisions of individuals in the rural areas of Vietnam, we conducted dataset mining and performed regression model analysis in the form of panel data. As control variables, we employed the variable of social capital, which is measured by an individual’s network, as well as demographic characteristics of individuals and households. We discovered that when a household is in financial distress, social networks such as linkages or asking for aid from others often enhance individual capacity. Individuals with a large social network outside of their immediate area are more inclined to relocate to the location where their connectors live. Individual participation and degree of participation in the organizational community, on the other hand, have little bearing on the likelihood of migration. In addition, this research examines theories and empirical research on the relationship between social capital and migration. Based on our research findings, we have recommended some measures to boost the efficiency of social capital and migration in rural areas of Vietnam through local government solutions.
Phenolic Constituents from Balanophora laxiflora with their Anti-inflammatory and Cytotoxic Effects
Nguyen Thi Hong Anh,Nguyen Thuy Duong,Pham Duc Vinh,Do Thi Ha 한국생약학회 2021 Natural Product Sciences Vol.27 No.1
Balanophora laxiflora Hemsl. (Balanophoraceae) is a traditional medicinal plant with a diverse array of biological activities. In our exploration of new bioactive constituents from B. laxiflora, we isolated five compounds, including a new lignan, balanophorone (5), and four known phenolic compounds (1–4). The chemical structures of these compounds were determined by extensive spectroscopic analyses, including 1D and 2D NMR, HR-ESI-MS, and CD. In addition, we evaluated the effects of each of the isolates (1–5) on the messenger RNA expression levels of tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells. Compound 2 showed significant inhibition of LPS-induced COX-2 and TNF-α expression in RAW 264.7 macrophages, while compound 4 showed moderate cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells, with IC50 values of 18.3 and 30.7 μM, respectively. No significant effects on the viability of normal mammary epithelial cells were observed.
Nguyen Thu Uyen,Dao Van Cuong,Pham Dieu Thuy,Luu Hong Son,Nguyen Thi Ngan,Nguyen Hung Quang,Nguyen Duc Tuan,In-ho Hwang 한국축산식품학회 2023 한국축산식품학회지 Vol.43 No.4
Myogenesis and adipogenesis are the important processes determining the muscle growth and fat accumulation livestock, which ultimately affecting their meat quality. Hanwoo is a popular breed and its meat has been exported to other countries. The objective of this study was to compare the myogenesis and adipogenesis properties in satellite cells, and meat quality between Hanwoo and Vietnamese yellow cattle (VYC). Same 28-months old Hanwoo (body weight: 728±45 kg) and VYC (body weight: 285±36 kg) steers (n=10 per breed) were used. Immediately after slaughter, tissue samples were collected from longissimus lumborum (LL) muscles for satellite cells isolation and assays. After 24 h post-mortem, LL muscles from left carcass sides were collected for meat quality analysis. Under the same in vitro culture condition, the proliferation rate was higher in Hanwoo compared to VYC (p<0.05). Fusion index was almost 3 times greater in Hanwoo (42.17%), compared with VYC (14.93%; p<0.05). The expressions of myogenesis (myogenic factor 5, myogenic differentiation 1, myogenin, and myogenic factor 6)- and adipogenesis (peroxisome proliferator-activated receptor gamma)-regulating genes, and triglyceride content were higher in Hanwoo, compared with VYC (p<0.05). Hanwoo beef had a higher intramuscular fat and total monounsaturated fatty acids contents than VYC beef (p<0.05). Whilst, VYC meat had a higher CIE a* and total polyunsaturated fatty acids content (p<0.05). Overall, there was a significant difference in the in vitro culture characteristics and genes expression of satellite cells, and meat quality between the Hanwoo and VYC.
Leaching of Black Dross by Hydrochloric Acid Solutions
Thi Thuy Nhi Nguyen,Thi Hong Nguyen,이만승 한국자원리싸이클링학회 2017 資源 리싸이클링 Vol.26 No.6
Leaching of black dross with HCl solution was conducted to find a suitable process to recover valauble materials. In this work, the leaching behavior of the components was compared between black dross and the residues after water treatment. All the components except TiO2 in the black dross were dissolved by HCl solution in the experimental ranges. Treatment of the black dross with water to recover the salts has negative effect on the leaching of Al2O3, MgO and SiO2. The reactions occuring during the leaching were discussed. At an optimum leaching condition of 3 M HCl and 90oC, the leaching percentage of Al2O3, MgO and SiO2 was 85, 100 and 40%, respectively.
The Effect of Trade Integration on Business Cycle Synchronization in East Asia
NGUYEN, Vinh Thi Hong,HOANG, Thuy Thi Thanh,NGUYEN, Sang Minh Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.8
The paper aims to investigate the impact of trade integration on business cycle synchronization for the East Asian countries during 2005- 2017 based on the endogeneity hypothesis of Optimum Currency Area criteria. We test the determinants of business cycles by calculating bilateral trade, financial integration, and business cycle synchronization. Applying the system Generalized Method of Moments for dynamic panel data models, the results show that business cycle synchronization is highly associated with trade and financial integration. These findings confirm the endogeneity hypothesis that more trade integration will mitigate asymmetric shocks, and have a positive impact on the business cycle synchronization. The increased trade intensity and financial linkage lead to more correlated business cycles in East Asia. Apart from trade and financial integration, the trade structure differential, monetary policy similarity also influence the business cycle comovement. The significantly negative impact of trade structure differential on business cycle synchronization suggests that countries with less similar structures are more likely to undergo asymmetric shocks. The results also indicate that monetary policy matters for output comovement. This study recommends that the East Asian countries should focus on bilateral trade as well as financial integration with each other to reap benefits from the integration process.
Thi Thu Ha Nguyen,Hiep-Hung Pham,Ngoc Linh Dao,Hong Hanh Dao,Thi Thuy Nuong Duong,Thi Ha Chu The International Academy of Global Business and T 2023 The International Academy of Global Business and T Vol.19 No.6
Purpose - Based on the extended theory of planned behavior (TPB), this study aims to investigate the antecedents of online purchase behavior toward organic cosmetics for Generation Z (Gen Z) and Millennials by focusing on the impact of subjective norms, health consciousness, trust, attitude, and intention. Design/Methodology/Approach - A survey method using an online questionnaire was adopted to collect data from 341 Gen Z and Millennial consumers in Vietnam. The collected data were then analyzed using structural equation modeling (SEM). Findings - The results of the study showed that, among antecedents, that attitude has the strongest impact on the purchase intention of online customers toward organic cosmetics, followed by trust and subjective norms. Interestingly, health consciousness has also been found to significantly impact Gen Z and Millennial online purchase intention toward organic cosmetics. Research Implications - This paper extends the academic literature on purchase intention toward green products. More importantly, to the best of the authors’ knowledge, this study is among the first to examine the antecedents of online purchase intention toward the organic cosmetics of Gen Z and Millennials in an emerging economy, Vietnam. The findings offer important implications for cosmetics companies, retailers, and other stakeholders to have deeper insights into factors influencing young Vietnamese consumer intentions toward purchasing organic beauty products online.
Perception of Organizational Support to Lecturers' Research Motivation: The Case of Vietnam
NGUYEN, Thanh Do,BUI, Thi Hong Viet,NGUYEN, Thi Le Thuy,TRAN, Manh Dung,TRAN, Thi Kim Nhung Korea Distribution Science Association 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.2
The aim of this study is to test the direct relationship and the indirect relationship of the perceived organizational support to lecturers' research motivation through "expectation" factor (belief in ability). The subject of the study is lecturers who are teaching and researching in the fields of economic, management and business administration at universities in Vietnam. The questionnaires were directly sent to lecturers at scientific conferences, sent out via e-mail and Facebook, the questionnaires were designed on google docs and directly sent to the lecturers. The detailed sample includes 475 respondents. The results of a quantitative analysis with a research sample of 475 lecturers in economics universities in Vietnam show that it is impossible to separate between lecturers' research ability and motivation. Lecturers with increased expectation (in terms of capability) also have increased research motivation, and vice versa. At the same time, the research also affirms the role of perceived organizational support, which not only has a direct positive impact on research motivation, but also increases expectation, thus, indirectly influence research motivation. This suggests that the solution for managers who want to improve research motivation by having measures to increase the expectation for the lecturers through various forms of organizational support.