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Taufiq Ahmad,신영민,이진규,신혁준,Sajeesh Kumar Madhurakart Perikamana,신흥수 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.67 No.-
Here, we engineered spheroids by using ECM mimicking nano-fragments (NFs) with fibroblasts and investigated their effect on proliferation and cell/ECM interactions. NF incorporation resulted in formation of a stable spheroid, which improved proliferation and viability of cells by assisting oxygen transport confirmed by LOX-1 staining. In addition, hypoxic and apoptotic genes were significantly downregulated in spheroids with PD-NFs. Furthermore, ECM and cell junction proteins were highly expressed. Overall, our findings suggest that incorporation of NFs within spheroids for assembly with various cell types can be an alternative approach for 3D cell culture in many applications.
Taufiq Carnegie Dawood,Alamsyah Alwi,Rustam Effendi,Muhammad Abrar 대한산업공학회 2022 Industrial Engineeering & Management Systems Vol.21 No.2
Open economies’ banking systems are prone to financial stability issues and global crises. Most financing to the pri-vate sector is channeled through bank credit, and Islamic banking is expanding worldwide. This study’s novelty com-pared to previous studies is investigating whether the international bank lending channel, measured by foreign interest rate changes, and exchange rate dynamics influences domestic Islamic bank credit vis-à-vis conventional bank credit . In Islamic banking, transactions based on interest are prohibited and hence should not influence Islamic bank credit. Employing the NARDL framework, this study found that Islamic bank credit is negatively related to foreign interest rates. In addition, Islamic bank credit is found to be more significantly affected by the international bank lending channel than conventional bank credit. Furthermore, the exchange rate negatively influences domestic Islamic bank credit more significantly than conventional bank credit. The findings are in contrast with parts of the literature, which insinuates that Islamic banking is immune to weaknesses of conventional banking system. For recommendations, in formulating policies aimed at financial stability, authorities must consider the international bank lending channel, exchange rate dynamics and take into account the different relationships these variables have with Islamic, and conventional bank credit.
Ahmad, Taufiq,Byun, Hayeon,Lee, Jinkyu,Madhurakat Perikamana, Sajeesh Kumar,Shin, Young Min,Kim, Eun Mi,Shin, Heungsoo Elsevier 2020 Biomaterials Vol.230 No.-
<P><B>Abstract</B></P> <P>Although stem cell spheroids offer great potential as functional building blocks for bottom-up bone tissue engineering, delivery of bioactive signals remain challenging. Here, we engineered adenosine-ligand-modified fiber fragments to create a 3D cell-instructive microenvironment for bone. Briefly, the Poly(ι-lactic acid) (PLLA) nanofiber sheet was partially degraded into fragmented fibers (FFs) through aminolysis and adenosine was stably incorporated via one-step polydopamine coating. The SEM and XPS analysis demonstrated that polydopamine assisted adenosine coating efficiency was significantly increased, which led to high coating efficiency of adenosine and its significant retention. The engineered fibers were then assembled into stable spheroids with human-adipose-derived stem cells (hADSCs). The adenosine in the spheroids effectively stimulated A2bR (1.768 ± 0.08) signaling, which further significantly induced the expression of osteogenic markers such as Runx2 (3.216 ± 0.25), OPN (4.136 ± 0.14), OCN (10.16 ± 0.34), and OSX (2.27 ± 0.11) with improved mineral deposition (1.375 ± 0.05 μg per spheroid). In contrast, the adipogenic differentiation of hADSCs was significantly suppressed within the engineered spheroids. Transplantation of engineered spheroids strongly induced osteogenic differentiation of hADSCs in ectopic subcutaneous tissue. Finally, the bone regeneration was significantly enhanced by implanting AP-FF group (59.97 ± 18.33%) as compared to P-FF (27.96 ± 11.14) and defect only (7.97 ± 3.76%). We propose that stem cell spheroids impregnated with engineered fibers enabling adenosine delivery could be promising building blocks for a bottom-up approach to create large tissues for regeneration of damaged bone.</P>
Ahmad, Taufiq,Shin, Hyeok Jun,Lee, Jinkyu,Shin, Young Min,Perikamana, Sajeesh Kumar Madhurakat,Park, So Yeon,Jung, Hyun Suk,Shin, Heungsoo Elsevier 2018 ACTA BIOMATERIALIA Vol.74 No.-
<P><B>Abstract</B></P> <P>Development of a bone-like 3D microenvironment with stem cells has always been intriguing in bone tissue engineering. In this study, we fabricated composite spheroids by combining functionalized fibers and human adipose-derived stem cells (hADSCs), which were fused to form a 3D mineralized tissue construct. We prepared fragmented poly (ι-lactic acid) (PLLA) fibers approximately 100 μm long by partial aminolysis of electrospun fibrous mesh. PLLA fibers were then biomineralized with various concentrations of NaHCO<SUB>3</SUB> (0.005, 0.01, and 0.04 M) to form mineralized fragmented fibers (mFF1, mFF2, and mFF3, respectively). SEM analysis showed that the minerals in mFF2 and mFF3 completely covered the fiber surface, and surface chemistry analysis confirmed the presence of hydroxyapatite peaks. Additionally, mFFs formed composite spheroids with hADSCs, demonstrating that the cells were strongly attached to mFFs and homogeneously distributed throughout the spheroid. <I>In vitro</I> culture of spheroids in the media without osteogenic supplements showed significantly enhanced expression of osteogenic genes including Runx2 (20.83 ± 2.83 and 22.36 ± 2.18 fold increase), OPN (14.24 ± 1.71 and 15.076 ± 1.38 fold increase), and OCN (4.36 ± 0.41 and 5.63 ± 0.51 fold increase) in mFF2 and mFF3, respectively, compared to the no mineral fiber group. In addition, mineral contents were significantly increased at day 7. Blocking the biomineral-mediated signaling by PSB 603 significantly down regulated the expression of these genes in mFF3 at day 7. Finally, we fused composite spheroids to form a mineralized 3D tissue construct, which maintained the viability of cells and showed pervasively distributed minerals within the structure. Our composite spheroids could be used as an alternative platform for the development of <I>in vitro</I> bone models, <I>in vivo</I> cell carriers, and as building blocks for bioprinting 3D bone tissue.</P> <P><B>Statement of Significance</B></P> <P>This manuscript described our recent work for the preparation of biomimeral-coated fibers that can be assembled with mesenchymal stem cells and provide bone-like environment for directed control over osteogenic differentiation. Biomineral coating onto synthetic, biodegradable single fibers was successfully carried out using multiple steps, combination of template protein coating inspired from mussel adhesion and charge-charge interactions between template proteins and mineral ions. The biomineral-coated single micro-scale fibers (1–2.5 μm in diameter) were then assembled with human adipose tissue derived stem cells (hADSCs). The assembled structure exhibited spheroidal architecture with few hundred micrometers. hADSCs within the spheroids were differentiated into osteogenic lineage <I>in vitro</I> and mineralized in the growth media. These spheroids were fused to form <I>in vitro</I> 3D mineralized tissue with larger size.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Ahmad, Taufiq,Parray, Shabir Ahmad,Ahmad, Naseem,Khan, Javed Ahmad,Zohaib, Sharique Cellmed Orthocellular Medicine and Pharmaceutical 2019 셀메드 (CellMed) Vol.9 No.1
Bronchial asthma is one of the most common chronic diseases globally and currently affects approximately 300 million people worldwide. As per scientific data, approximately 10-12% of adults; and 15% of children are affected by the disease. The increasing global prevalence of the disease imposes a high health care costs into its mechanisms and treatment. Unani system of medicine (USM) is a well known traditional therapy for number of diseases since ancient times. Time has proved that USM has a special role in treatment of chronic diseases, due its special Usooleillaj (Line of treatment). Dhiq al-Nafas (bronchial asthma) is also considered as a chronic disease. Since ancient times, the disease was cured by number of single as well as compound formulations by renowned Unani scholars. This review provides a comprehensive summary of medicinal plant throughout the world, with reference to renowned Unani scholars and physicians for treatment of Dhiq al-Nafas. A number of Unani single and compound drugs, highly efficacious and safe drugs are available for the asthma. The data were taken from classical literature of USM, Modern reference books & electronic journals. The recent information was collected from different authentic search engines. This review will provide the centuries therapeutic information's of classical literature and recent scientific studies of 12 herbal drugs mentioned in USM, which will help the academia, clinicians, research scholars and post graduate students from Unani Medicine, Traditional & Complementary Medicine and other related disciplines, having research interest or work in the Unani medicine.