http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Inhibition of Dll4/Notch1 pathway promotes angiogenesis of Masquelet’s induced membrane in rats
Qian Tang,Haimin Jin,Minji Tong,Gang Zheng,Zhongjie Xie,Shangkun Tang,Jialei Jin,Ping Shang,Huazi Xu,Liyan Shen,Yu Zhang,Haixiao Liu 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
The Masquelet’s induced membrane technique for repairing bone defects has been demonstrated to be a promising treatment strategy. Previous studies have shown that the vessel density of induced membrane is decreased in the late stage of membrane formation, which consequently disrupts the bone healing process. However, relatively little is known about certain mechanisms of vessel degeneration in the induced membrane tissue and whether promotion of angiogenesis in induced membranes can improve bone regeneration. Here, we showed that the Delta-like ligand 4/ Notch homolog 1 (Dll4/Notch1) pathway was relatively activated in the late stage of induced membrane, especially at the subcutaneous site. Then, DAPT, a classical γ-secretase inhibitor, was applied to specifically inhibit Notch1 activation, followed by up-regulation of vascular endothelial growth factor receptor 2 (VEGFR2) and CD31 expression. DAPTmodified induced membranes were further confirmed to contribute to bone regeneration after autogenous bone grafting. Finally, in vitro experiments revealed that knocking down Notch1 contributed to the functional improvement of endothelial progenitor cells (EPCs) and that DAPT-treated induced membrane tissue was more favorable for angiogenesis of EPCs compared with the vehicle group. In conclusion, the present findings demonstrate that Dll4/ Notch1 signaling is negatively associated with the vessel density of induced membrane. Pharmacological inhibition of Notch1 attenuated the vessel degeneration of induced membrane both in vitro and in vivo, which consequently improved bone formation at the bone defect site and graft resorption at the subcutaneous site.
Tang, Shu-Kun,Zhi, Xiao-Yang,Wang, Yun,Wu, Jin-Yuan,Lee, Jae-Chan,Kim, Chang-Jin,Lou, Kai,Xu, Li-Hua,Li, Wen-Jun Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.9
<P>A Gram-staining-positive, facultatively anaerobic, non-motile and moderately halophilic actinobacterium, designated YIM 93306<SUP>T</SUP>, was isolated from a salt lake in Xinjiang province, north-west China, and subjected to a polyphasic taxonomic study. Strain YIM 93306<SUP>T</SUP> grew in the presence of 2-16 % (w/v) NaCl and did not grow without NaCl. The peptidoglycan type was A4<I>α</I> with an l-Lys-l-Glu interpeptide bridge. The whole-cell sugars were glucosamine, arabinose, mannose and two unknown sugars. The predominant menaquinone was MK-8(H4). The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phosphoglycolipid and one unknown phospholipid. The DNA G+C content was 68.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 93306<SUP>T</SUP> fell within the radius of the suborder <I>Micrococcineae</I>. Its closest phylogenetic neighbour was the type strain of <I>Ruania albidiflava</I> (AS 4.3142<SUP>T</SUP>; 96.2 % 16S rRNA gene sequence similarity), the sole recognized species of the genus <I>Ruania</I>. Sequence similarities between strain YIM 93306<SUP>T</SUP> and members of other genera of the suborder <I>Micrococcineae</I> were <95.2 %. On the basis of phylogenetic analysis, phenotypic characteristics and chemotaxonomic differences, a novel genus and species, <I>Haloactinobacterium album</I> gen. nov., sp. nov., is proposed. The type strain of the species is YIM 93306<SUP>T</SUP> (=DSM 21368<SUP>T</SUP> =KCTC 19413<SUP>T</SUP> =CCTCC AB 208069<SUP>T</SUP>). Based on phylogenetic characteristics and 16S rRNA gene signature nucleotide patterns, the genera <I>Ruania</I> and <I>Haloactinobacterium</I> gen. nov. are proposed to belong to a novel family, <I>Ruaniaceae</I> fam. nov.</P>
Overexpression of IbMPK3 increases low-temperature tolerance in transgenic sweetpotato
Jin Rong,Kim Ho Soo,Yu Tao,Liu Ming,Yu Wenhui,Zhao Peng,Zhang Aijun,Zhang Qiangqiang,Liu Yaju,Cao Qinghe,Kwak Sang-Soo,Tang Zhonghou 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.1
Sweetpotato is an important crop that is very sensitive to low temperatures. Mitogen-activated protein kinase (MAPK) is involved in plant growth and development and is responsive to many environmental stresses. IbMPK3 is strongly regulated by low-temperature stress. We studied the function of IbMPK3 and how it may enhance the adaptability of sweetpotato plants to low-temperature stress. Transgenic sweetpotato plants overexpressing IbMPK3 were generated, and three trans- genic lines with the highest expression level of IbMPK3 were used for low-temperature tests. Transgenic plants exposed to low-temperature stress had less damage associated with higher photosynthesis efficiency and less cell membrane damage. IbMPK3-overexpressing transgenic plants could modulate reactive oxygen species (ROS) metabolism with lower levels of O2− and H2O2 accumulation and higher enzymatic activities than WT plants. Transcript expression levels of some ROS- related genes (IbCAT, IbCu-ZnSOD, and IbCAT) and stress-responsive genes (IbP3B and IbCOR27) were significantly upregulated in transgenic plants compared to WT. These results indicate that IbMPK3 has an important role in sweetpotato response to low temperatures.
Tang, Yujiao,Fan, Meiqi,Choi, Young-Jin,Choi, Eun-Ju,Moon, Sang-Ho,Debnath, Trishna,Yu, Yonghai,Lee, Il Nam,Kim, Eun-Kyung Hindawi Limited 2018 Journal of chemistry Vol.2018 No.-
<P>We previously discovered the antioxidant and antiprostate cancer effects of antler extract (AE), but whether it inhibits cisplatin- (Cis-) induced toxicity has not been investigated. In this study, the effect of AE on Cis-induced side effects in the kidney and liver using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide-based cytotoxicity and cell cycle assays in prostate cancer PC-3 cells in vitro is investigated. Furthermore, we used a xenograft mouse model of the same cells to examine the in vivo effects and mechanisms of action. Cis and Cis + AE treatment attenuated prostate cancer cell growth by inducing apoptosis in vitro. Cis + AE stimulated cleaved caspases 3, 7, and 9 and polyadenosine diphosphate ribose polymerase expression. Cis + AE treatment for 1 week significantly increased the superoxide dismutase and catalase antioxidant activity while thiobarbituric acid reactive substances decreased. The histopathological damage and tumor necrosis factor-α, interleukin- (IL-) 1<I>β</I> and IL-6, cyclooxygenase-2, and inducible nitric oxide synthase expression in the kidney and liver tissue decreased. Therefore, AE likely possesses antiprostate cancer activity and inhibits Cis toxicity.</P>
Five miRNAs as Novel Diagnostic Biomarker Candidates for Primary Nasopharyngeal Carcinoma
Tang, Jin-Feng,Yu, Zhong-Hua,Liu, Tie,Lin, Zi-Ying,Wang, Ya-Hong,Yang, La-Wei,He, Hui-Juan,Cao, Jun,Huang, Hai-Li,Liu, Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18
MicroRNAs (miRNAs) play an essential role in the development and progression of nasopharyngeal carcinomas (NPC). Despite advances in the field of cancer molecular biology and biomarker discovery, the development of clinically validated biomarkers for primary NPC has remained elusive. In this study, we investigated the expression and clinical significance of miRNAs as novel primary NPC diagnostic biomarkers. We used an array containing 2, 500 miRNAs to identify 22 significant miRNAs, and these candidate miRNAs were validated using 67 fresh NPC and 25 normal control tissues via quantitative real-time PCR (qRT-PCR). Expression and correlation analyses were performed with various statistical approaches, in addition to logistic regression and receiver operating characteristic curve analyses to evaluate diagnostic efficacy. qRT-PCR revealed five differentially expressed miRNAs (miR-93-5p, miR-135b-5p, miR-205-5p and miR-183-5p) in NPC tissue samples relative to control samples (p<0.05), with miR-135b-5p and miR-205-5p being of significant diagnostic value (p<0.01). Moreover, comparison of NPC patient clinicopathologic data revealed a negative correlation between miR-93-5p and miR-183-5p expression levels and lymph node status (p<0.05). These findings display an altered expression of many miRNAs in NPC tissues, thus providing information pertinent to pathophysiological and diagnostic research. Ultimately, miR-135b-5p and miR-205-5p may be implicated as novel NPC candidate biomarkers, while miR-93-5p, miR-650 and miR-183-5p may find application as relevant clinical pathology and diagnostic candidate biomarkers.
Face Sketch Synthesis Based on Local and Nonlocal Similarity Regularization
Tang, Songze,Zhou, Xuhuan,Zhou, Nan,Sun, Le,Wang, Jin Korea Information Processing Society 2019 Journal of information processing systems Vol.15 No.6
Face sketch synthesis plays an important role in public security and digital entertainment. In this paper, we present a novel face sketch synthesis method via local similarity and nonlocal similarity regularization terms. The local similarity can overcome the technological bottlenecks of the patch representation scheme in traditional learning-based methods. It improves the quality of synthesized sketches by penalizing the dissimilar training patches (thus have very small weights or are discarded). In addition, taking the redundancy of image patches into account, a global nonlocal similarity regularization is employed to restrain the generation of the noise and maintain primitive facial features during the synthesized process. More robust synthesized results can be obtained. Extensive experiments on the public databases validate the generality, effectiveness, and robustness of the proposed algorithm.
Two new tirucallane triterpenoids from the leaves of Aquilaria sinensis
Jin Tang Cheng,Ya Qiong Han,Juan He,Xing De Wu,Liao Bin Dong,Li Yan Peng,Yan Li,Qin Shi Zhao 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.9
Two new tirucallane triterpenoids, aquilacallanesA–B (1–2), together with 15 known compounds(3–17) were isolated from the leaves of Aquilaria sinensis. The structures of these new compounds were elucidated onthe basis of extensive spectroscopic analyses. All compoundswere evaluated for their cytotoxic activity againstfive human cancer cell lines. The known compounds,ursolic acid (7) and 5,7,40-trimethoxyflavone (14), exhibitedweak cytotoxic activity against some cells.
Tang, Jin-Niu,Pan, Gang-Hong,Li, Long,Tian, Wei-Man,Huang, Zhong-Jing Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.2
Two novel binuclear metal-organic coordination complexes [$Cd_2(Hdpa)_4(bpy)_2$] (1), [$Dy_2(dpa)_2(bpy)_2(NO_3)_2-(H_2O)_2$](bpy) (2) (where $H_2dpa$ = biphenyl-2,2'-dicarboxylic acid, bpy = 2,2'-bipyridine) have been synthesized under hydrothermal conditions and characterized by single crystal X-ray diffraction, spectral method (IR), elemental analysis (EA), powder X-ray diffraction (XRD), electronic spectra (UV-vis), fluorescent in the solid state and thermogravimetric analysis (TGA). Complexes 1-2 crystallizes isomorphously in the Triclinic space group P-1. The ${\pi}-{\pi}$ stacking interactions and hydrogen-bonds play a vital role in determining the crystal packing and construction of the extended 3-D supramolecular network.
Jin-Wei Yang,Cui Li,Xiao-Peng He,Hong Zhao,Li-Xin Gao,Wei Zhang,Xiao-Xin Shi,Yun Tang,Jia Li,Guo-Rong Chen 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.11
The incorporation of microwave irradiation with the prevalent “click chemistry” is currently of considerable synthetic interest. We describe here the introduction of such laboratorial shortcut into carbohydrate-based drug discovery,resulting in the rapid formation of a series of triazole-linked salicylic β-D-O-glycosides with biological activities. All “clicked” products were achieved in excellent yields (≈ 90%) within only a quarter. In addition, based on the structural characteristics of the afforded glycomimetics, their inhibitory activities were evaluated toward protein tyrosine phosphatases 1B (PTP1B) and a panel of homologous protein tyrosine phosphatases (PTPs). Docking simulation was also conducted to plausibly propose binding modes of this glycosyl salicylate series with the enzymatic target.