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In vivo genome editing targeted towards the female reproductivesystem
Masahiro Sato,Masato Ohtsuka,Shingo Nakamura,Takayuki Sakurai,Satoshi Watanabe,Channabasavaiah B. Gurumurthy 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.9
The discovery of sequence specific nucleases such as ZFNs, TALENs, and CRISPR/Cas9 has revolutionized genome editing. The CRISPR/Cas9 system has particularly emerged as a highly simple and efficient approach towards generating genome-edited animal models of most of the experimental species. The limitation of these novel genome editing tools is that, till date, they depend on traditional pronuclear injection (PI)-based transgenic technologies developed over the last three decades. PI requires expensive micromanipulator systems and the equipment operators must possess a high level of skill. Therefore, since the establishment of PI-based transgenesis, various research groups worldwide have attempted to develop alternative and simple gene delivery methods. However, owing to the failure of chromosomal integration of the transgene, none of these methods gained the level of confidence as that by the PI method in order to be adapted as a routine approach. The recently developed genome editing systems do not require complicated techniques. Therefore, presently, attention is being focused on non-PIbased gene delivery into germ cells for simple and rapid production of genetically engineered animals. For example, a few reports during the previous 1–2 years demonstrated the use of electroporation (EP) in isolated zygotes that helped to overcome the absolute dependency on PI techniques. Recently, another breakthrough technology called genome editing via oviductal nucleic acids delivery (GONAD) that directly delivers nucleic acids into zygotes within the oviducts in situ was developed. This technology completely relieves the bottlenecks of animal transgenesis as it does not require PI and ex vivo handling of embryos. This review discusses in detail the in vivo gene delivery methods targeted towards female reproductive tissues as these methods that have been developed over the past 2–3 decades can now be re-evaluated for their suitability to deliver the CRISPR/Cas9 components to produce transgenic animals. This review also provides an overview of the latest advances in CRISPR-enabled delivery technologies that have caused paradigm shifts in animal transgenesis methodologies.
Masatoshi Sato,Yoshiaki Kobayashi,Takayuki Kawamata,Yukio Yasui,Kazunori Suzuki,M. Itoh,Ryoichi Kajimoto,Kazuhiko Ikeuchi,M. Arai,Phillipe Bourges 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.12
To identify the superconducting symmetry of Fe-based superconductors, we studied effects ofnonmagnetic-impurities on Tc, magnetic excitation spectra 00 and NMR 1/T1 − T curve, whichare sensitive to the relative signs between the order parameters on the disconnected Fermi surfacesin reciprocal space, because the symmetry is closely connected with the pairing mechanism:If the signs are opposite (symmetry S±), the mechanism is considered to be a magneticone, but when the signs are the same (symmetry S++), a novel mechanism is plausible becausethe ordinary phonon mechanism cannot realize the Tc value as high as 55 K found in Ln1111(Ln=lanthanide). Results are as follows: (a) The very small rates of Tc-suppression by impurityatoms M of LnFe1−yMyAs0.89−xF0.11+x (M=Ni, Co, Ru) can be explained only by S++. (b) The00-data for Ba(Fe, Co)2As2 (Tc 23 K) and Ca-Fe-Pt-As crystals seem to be well explained byS++ rather than by S±. (c) The nuclear magnetic resonance data can be consistently understoodby S++, too. These results suggest the S++ symmetry and a novel pairing mechanism, which canbe considered to be related to the elastic softening of C66 induced by the orbital fluctuation of thesystem.
Three-Phase PWM Inverter and Rectifier with Two-Switch Auxiliary Resonant DC Link Snubber-Assisted
Nagai Shinichiro,Sato Shinji,Matsumoto Takayuki The Korean Institute of Power Electronics 2005 JOURNAL OF POWER ELECTRONICS Vol.5 No.3
In this paper, a new conceptual circuit configuration of a 3-phase voltage source, soft switching AC-DC-AC converter using an IGBT module, which has one ARCPL circuit and one ARDCL circuit, is presented. In actuality, the ARCPL circuit is applied in the 3-phase voltage source rectifier side, and the ARDCL circuit is in the inverter side. And more, each power semiconductor device has a novel clamp snubber circuit, which can save the power semiconductor device from voltage and current across each power device. The proposed soft switching circuits have only two active power semiconductor devices. These ARCPL and ARDCL circuits consist of fewer parts than the conventional soft switching circuit. Furthermore, the proposed 3-phase voltage source soft switching AC-DC-AC power conversion system needs no additional sensor for complete soft switching as compared with the conventional 3-phase voltage source AC-DC-AC power conversion system. In addition to this, these soft switching circuits operate only once in one sampling term. Therefore, the power conversion efficiency of the proposed AC-DC-AC converter system will get higher than a conventional soft switching converter system because of the reduced ARCPL and ARDCL circuit losses. The operation timing and terms for ARDCL and ARCPL circuits are calculated and controlled by the smoothing DC capacitor voltage and the output AC current. Using this control, the loss of the soft switching circuits are reduced owing to reduced resonant inductor current in ARCPL and ARDCL circuits as compared with the conventional controlled soft switching power conversion system. The operating performances of proposed soft switching AC-DC-AC converter treated here are evaluated on the basis of experimental results in a 50kVA setup in this paper. As a result of experiment on the 50kVA system, it was confirmed that the proposed circuit could reduce conduction noise below 10 MHz and improve the conversion efficiency from 88. 5% to 90.5%, when compared with the hard switching circuit.