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Gradient index lens based combined two-photon microscopy and optical coherence tomography
Wang, Taejun,Li, Qingyun,Xiao, Peng,Ahn, Jinhyo,Kim, Young Eun,Park, Youngrong,Kim, Minjun,Song, Miyeoun,Chung, Euiheon,Chung, Wan Kyun,Ahn, G-One,Kim, Sungjee,Kim, Pilhan,Myung, Seung-Jae,Kim, Ki Hea The Optical Society 2014 Optics express Vol.22 No.11
Park, Youngrong,Ryu, Yeon-Mi,Jung, Yebin,Wang, Taejun,Baek, Yeonggyeong,Yoon, Yeoreum,Bae, Sang Mun,Park, Joonhyuck,Hwang, Sekyu,Kim, Jaeil,Do, Eun-Ju,Kim, Sang-Yeob,Chung, Euiheon,Kim, Ki Hean,Kim, S American Chemical Society 2014 ACS NANO Vol.8 No.9
<P>The detection of colon cancer using endoscopy is widely used, but the interpretation of the diagnosis is based on the clinician’s naked eye. This is subjective and can lead to false detection. Here we developed a rapid and accurate molecular fluorescence imaging technique using antibody-coated quantum dots (Ab–QDs) sprayed and washed simultaneously on colon tumor tissues inside live animals, subsequently excited and imaged by endoscopy. QDs were conjugated to matrix metalloproteinases (MMP) 9, MMP 14, or carcinoembryonic antigen (CEA) Abs with zwitterionic surface coating to reduce nonspecific bindings. The Ab–QD probes can diagnose tumors on sectioned mouse tissues, fresh mouse colons stained <I>ex vivo</I> and also <I>in vivo</I> as well as fresh human colon adenoma tissues in 30 min and can be imaged with a depth of 100 μm. The probes successfully detected not only cancers that are readily discernible by bare eyes but also hyperplasia and adenoma regions. Sum and cross signal operations provided postprocessed images that can show complementary information or regions of high priority. This multiplexed quantum dot, spray-and-wash, and endoscopy approach provides a significant advantage for detecting small or flat tumors that may be missed by conventional endoscopic examinations and bestows a strategy for the improvement of cancer diagnosis.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2014/ancac3.2014.8.issue-9/nn5009269/production/images/medium/nn-2014-009269_0012.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn5009269'>ACS Electronic Supporting Info</A></P>
Singha, Subhankar,Kim, Dokyoung,Moon, Hyunsoo,Wang, Taejun,Kim, Ki Hean,Shin, Youn Ho,Jung, Junyang,Seo, Eunseok,Lee, Sang-Joon,Ahn, Kyo Han American Chemical Society 2015 ANALYTICAL CHEMISTRY - Vol.87 No.2
<P>Hydrogen sulfide has emerged as an exciting endogenous gasotransmitter in addition to nitric oxide and carbon dioxide. Noninvasive detection methods for hydrogen sulfide thus become indispensable tools for studying its diverse roles in biological systems. Accordingly, fluorescent probes for hydrogen sulfide have received great attention in recent years. A practically useful fluorescent probe for bioimaging of hydrogen sulfide should be selective, sensitive, fast-responsive, biocompatible, observable in the biological optical window, and capable of deep-tissue imaging. These sensing properties, however, are extremely difficult to achieve at the same time. Disclosed here is the two-photon fluorescent probe that meets all of these criteria. The probe belongs to a Michael acceptor system, which raised a serious selectivity issue over the competing biothiols such as cysteine and glutathione. We have addressed the selectivity issue by optimizing the electronic and steric interactions between biothiols and the probe, in addition to achieving very high sensitivity, fast-response, and biocompatibility. Also, the sensing mechanism suggested in the literature was revised. The probe thus enables us to image the endogenously produced hydrogen sulfide with negligible interference from other biothiols in live cells. The excellent sensing properties of the probe combined with its capability of bioimaging thus make it a practically useful tool for further studying biological roles of hydrogen sulfide.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2015/ancham.2015.87.issue-2/ac503806w/production/images/medium/ac-2014-03806w_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac503806w'>ACS Electronic Supporting Info</A></P>
Non-ablative Fractional Thulium Laser Irradiation Suppresses Early Tumor Growth
Yoo, Su Woong,Park, Hee-Jin,Oh, Gyungseok,Hwang, Soonjoo,Yun, Misun,Wang, Taejun,Seo, Young-Seok,Min, Jung-Joon,Kim, Ki Hean,Kim, Eung-Sam,Kim, Young L.,Chung, Euiheon Optical Society of Korea 2017 Current Optics and Photonics Vol.1 No.1
In addition to its typical use for skin rejuvenation, fractional laser irradiation of early cancerous lesions may reduce the risk of tumor development as a byproduct of wound healing in the stroma after the controlled injury. While fractional ablative lasers are commonly used for cosmetic/aesthetic purposes (e.g., photorejuvenation, hair removal, and scar reduction), we propose a novel use of such laser treatments as a stromal treatment to delay tumorigenesis and suppress carcinogenesis. In this study, we found that non-ablative fractional laser (NAFL) irradiation may have a possible suppressive effect on early tumor growth in syngeneic mouse tumor models. We included two syngeneic mouse tumor models in irradiation groups and control groups. In the irradiation group, a thulium fiber based NAFL at 1927 nm was used to irradiate the skin area including the tumor injection region with 70 mJ/spot, while no laser irradiation was applied to the control group. Numerical simulation with the same experimental condition showed that thermal damage was confined only to the irradiation spots, sparing the adjacent tissue area. The irradiation groups of both tumor models showed smaller tumor volumes than the control group at an early tumor growth stage. We also detected elevated inflammatory cytokine levels a day after the NAFL irradiation. NAFL treatment of the stromal tissue could potentially be an alternative anticancer therapeutic modality for early tumorigenesis in a minimally invasive manner.
Kim, Dokyoung,Moon, Hyunsoo,Baik, Sung Hoon,Singha, Subhankar,Jun, Yong Woong,Wang, Taejun,Kim, Ki Hean,Park, Byung Sun,Jung, Junyang,Mook-Jung, Inhee,Ahn, Kyo Han American Chemical Society 2015 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.137 No.21
<P>Fluorescence imaging of tissues offer an essential means for studying biological systems. Autofluorescence becomes a serious issue in tissue imaging under excitation at UV-vis wavelengths where biological molecules compete with the fluorophore. To address this critical issue, a novel class of fluorophores that can :be excited at, similar to 900 nm under two-photon excitation conditions and emits in the red wavelength region (>= 600 nm) has been disclosed. The new pi-extended dipolar dye system, shows several advantageous features including minimal antofluorescence in tissue imaging and pronounced solvent-sensitive emission behavior, compared with a widely used two-photon absorbing dye, acedan. As an important application of the new dye system, one of the dyes was developed into a fluorescent probe for amyloicl-beta plaques, a key biomarker of Alzheimer's disease. The probe enabled in Vivo imaging of amyloid-beta plaques in a disease-model mouse, with negligible background signal. The new dye system has great potential for the development of other types of two-photon fluorescent probes and tags for imaging of tissues with minimal autofluorescence.</P>