Evaluation of genetic diversity of red pepper landraces (Capsicum annuum L.) from Bulgaria using SSR markers

Sejong Oh,Jeongran Lee,Gi An Lee,Jae Young Song,Tsvetelina,Lilia,Yeon Gyu Kim,Ju Hee Lee,Myung Chul Lee 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The amount of genetic variability of a species is essential for its survival and adaptation in different environments, and studies of genetic diversity using molecular markers are necessary to understand the genetic structure of a population and to orientate effective strategies of germplasm conservation. The aim of current study was to determine the SSR markers that can be used rapidly and reliably to evaluated the pepper of Bulgaria landraces, and applied the markers to assement of introduce genetic diversity of the pepper germplasm. We used 22 polymorphic microsatellite markers to analysis of genetic diversity within 61 pepper collection of Bulgaria landraces germplasm, all SSR primers pairs produced 80 polymorphic and reproducible amplification fragments. An average value of polymorphic information contents (PIC) were 0.334 with a range of 0.061 to 0.63. The mean values of observed (HO) and expected heterozygosity (HE) were 0.383 and 0.154, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into three distinct groups, which was the landrace, moderate and wilde type, genetic distance (GD) value was 0.540. An average day of flowering time was 53 days with a range of 45 to 60 days. The everage od fruit length and width were 9.38cm with a range 2.1 to 23.6cm, and 3.51cm with a range 0.6 to 8.9cm, respectively. Molecular data were complemented with morphological measurements according to the descriptor list for the pepper collection of Bulgaria landraces germplasm.

고농도의 beta-HCG를 분비하는 순수 미분화세포종

이태성,이동섭,육상수,이성운,정재형 대한산부인과학회 1990 Obstetrics & Gynecology Science Vol.33 No.7

미분화 세포종은 원시 배세포에서 기원된 드문 난소종양으로 순수 미분화세포종은 호르몬생성능이 없다고 알려져 왔으나 최근의 연구와 보고자들에 의하면 전자현미경, 면역 세포조직학, 면역조직화학, 효소조직화확, 방사선 면역분석법 등 그중에서 특히 전자현미경의 발달로, 면역조직화확, 효소조직화확, 방사선면역분석법 등 그중에서 특히 전자현미경의 발달로 이제까지 미분화세포종에서는 알려지지 않았던 미세구조들이 밝혀지고 그와함께 화확적, 면역학적, 면역방사선학적 분석법 등이 발달하면서 이형성의 여러 다양한 기능성 세포들이 있다는 것을 아고, 그에 따른 여러 단백질, 호르몬, 효소들이 분비된다는 것을 증명하였다. 또, 치료방법에 있어서도 보고자들에 따라 다른 주장을 하였으며 또 재발율이 높은 원인에 대해 보고자들이 복막과 임파절 전이를 중요시 하고 있지만 방사선치료나, 항암화확요법 등의 자극에 의한 다른 종양으로의 분화 혹은 전환, 현미경적조직검사시 부적절한 표본의 선택 등도 원인이 될 수 있다고하고 또, 전형적인 어느 한 종양의 조직소견을 보이면서 다른 종양과 아주 유사한 요소들이 있을 때 애매한 점이 있다고 하였다. 이러한 점을 통틀어 생각해 볼 때 미분화세포종의 여러 가지 임상적, 병리조직학적 특성과, 진단과 치료 등에 이르기까지 앞으로 많은 연구가 필요할 것으로 생각된다. 본 저자들은 10,000IU/mml이상의 고농도 beta-HCG를 분비한 전이성 순수 미분화세포종 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Dysgerminoma is a rare form of malignant germ cell tumor, occurs predominantly in child and young women. Pure dysgerminoma is known to usually non-functioning, but we experienced typical histogenesis of pure dysgerminoma with syncytiotrophoblasts and high beta-HCG were produced. In the study of germ cell tumor, the development of electron microscope, methods of histologic, immunologic, chemistry, cytologic and combined with each other recommands many thoughts and difficulties in our decisions. So we review of our case and literatures.

Push-shear Bond Strength between CAD/CAM Zirconia Ceramic Core and Zirconia Veneering Ceramics

Lee JH,Jung JH,Kim YK,Ji JH,Park IS,Lee MH,Bae TS 대한치과재료학회 2011 대한치과기재학회 학술대회 Vol.2011 No.5

거대아의 임상적 고찰

이태성,이두룡,윤성도,김종인,정철재,이무용 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.6

일반적으로 거대아라 함은 출생시 신생아의 체중이 4,000 gm이상인 경우를 말하나 각 문헌에 따라 기준체중에 대해 약간의 차이가 있다. 거대아의 원인으로는 큰 체격의 부모, 경산부, 모체 당뇨병, 과숙임신, 4,000 gm이상의 신생아를 분만한 경력이 있는 산모 등을 들 수 있다. 1991년 1월 1일부터 1993년 12월 31일까지 만 3년동안 계명대학교부속 동산의료원 산부인과에서 입원 분만한 총 13,334례중 출생시 태아체중이 4,000 gm이상인 거대아 및 그들의 산모를 대상으로 임상적 결과를 통계학적으로 조사, 분석하여 다음과 같은 결론을 얻었다. 1. 총 분만 13,334례중 4,000 gm이상의 거대아는 485례(3.64%)였으며, 4,500 gm이상의 거대아는 60례(0.45%)였다. 2. 산모의 평균연령은 28.90세, 평균 출산횟수는 1.73로서 대조군과 별 차이가 없었다. 3. 임신전의 평균체중은 55.14 kg이었으며 임신중 산모의 평균체중증가는 14.78 kg으로 나타났다. 4. 거대아의 평균 제태연령은 40.02주로 대조군에 비해 높게 나타났다. 5. 남녀 성비는 194.60:100.00으로 남아가 더 많았다. 6. Apgar score는 1분이 7.92, 5분이 8.99로 나타났고 대조군과 별 차이가 없었다. 7. 분만방법은 정상질식분만이 71.74%였고, 제왕절개분만이 28.26%였으며 제왕절개분만의 경우 대조군의 경우 보다 높게 나타났다. 8. 제왕절재분만의 적응증을 살펴보면 거대아 39례(25.32%), 전제왕절개술의 기왕력 35례(22.73%), 아두골반불균형 27례(17.53%)의 순이었다. 9. 산전진찰중 발견된 산모의 질환은 빈혈이 80례(14.68%)로 제일 많았으며, 임신성 고혈압이 12례(2.20%), 임신성 당뇨 2례(0.37%)를 보였다. 10. 분만증 산후 모성 합병증은 질열창이 24례(4.40%로 가장 많았으며 산후 출혈이 21례(3.85%)로 나타났다. 11. 신생아 합병증으로는 두혈종이 8례(1.47%)로 제일 많았고, 상완 신경총마비 3례(0.55%), 쇄골골절 1례(0.18%)가 있었으며 태아사망은 없었다. 결론적으로 저자들의 연구결과에 의하면, 거대아로 인한 여러제반문제를 피하기 위해 거대아ㅔ 대한 정확한 진단과 적절한 분만방법의 선택, 숙련된 의술, 거대아의 임신 및 분만시 일어날 수 있는 제반 문제에 미리 대비함으로서 산과적 합병증 및 모성, 신생아 이환율을 줄일 수 있을 것으로 사료된다. Since the delivery of fetus weighing 4,000 gm or more brings dangerous complication to mother and fetus during pregnancy, delivery and after delivery, this is one of the most important problem in obstetrics. We studied 545 cases of large babies weighing 4,000 gm or more among 13,334 cases of total deliveries during past 3 years from Jan. 1991 to Dec. 1993. The results were as flows: 1. The incidence of macrosomia weighing 4,000 gm or more was 3.64%, and that of macrosomia weighing 4,500 gm or more was 0.45%. 2. The highest age distribution of mother was found between 25 to 29 year of age. The mean age was 28.90 year. There was no tendency of the increase in the incidence of macrosomia as the parity increase. 3. Mean weight gain during pregnancy was 14.78Kg and mean weight of pregravida was 55.14Kg. 4. Mean gestational age during pregnancy was 40.02 weeks. 5. Male to female ratio was 194.60:100. 6. The Apgar score of the 1 minute was 7.92, and 5 minute was 8.99. 7. 71.74% of patients had normal vaginal delivery, and 28.26% were delivered by cesarean section. 8. The indications for cesarean sections; the most common indication was large baby (25.32%), previous C-section(22.73%), cephalopelvic disproportion(17.53%) in the order. 9. The most common maternal antepartum complication was anemia (14.68%) and others were pregnancy induced hypertension(2.20%), thyroid disease (0.55%), and gestational diabetes mellitus(0.37%). 10. The most common maternal complication of intrapartum and postpartum period were vaginal laceration (4.40%) and postpartum beeding(3.85%). 11. Neonatal complication in macrosomia were cephalhematoma (1.47%), brachial plexus paralysis (0.55%), and fracture of clavicle (0.14%). Perinatal death was not visible.

Surface Characteristics of Titanium Implant Modified by Blasting and Acid-etching

Lee KJ,Park IS,Choi SK,Ryoo GH,Park KB,Lee MH,Bae TS 대한치과재료학회 2011 대한치과기재학회 학술대회 Vol.2011 No.5

Remote Real Time Monitoring for Underground Contamination in Mongolia Using Electrical Impedance Tomography

Ts, Munkh-Erdene,Lee, Eunjung,Zhou, Liangdong,Lee, Kyoung Hun,Seo, Jin Keun Springer-Verlag 2016 Journal of nondestructive evaluation Vol.35 No.1

자궁내막증의 임상적 고찰

이태성,차순도,서영욱,이탁,이정호 대한산부인과학회 1990 Obstetrics & Gynecology Science Vol.33 No.6

1. 자궁내막증의 발생빈도는 개복수술을 한 예에서 2.8%였다. 2. 호발연령은 30대, 20대 순이었다. 3. 임상증상에 있어서 월경통 90.2%, 월경전 통증 80.0%, 성교통 29.2% 그리고 배변시의 통증이 36.9%나 되었다. 4. 불임증을 동반한 예가 49.2%였다. 5. 불임증을 동반한 예에서 불임기간이 5년이내가 68.8%, 6~10년이 21.9%였고, 불임을 동반하지 않았던 예에서는 최종임신에서 진단시까지의 기간이 6~10년이 36.4%, 11~15년이 33.3%로, 6년이상이 81.8%나 되었다. 6. 내진시의 소견으로 부속기에 고정된 종괴의 촉지가 44.7%, 자궁천골인대에 결절상 종괴 26.2%, 그리고 더그라스와에서의 경결이 15.4%촉지되었다. 7. 호발부위는 난소 76.9%, 자궁천골인대 52.3%, 자궁 35.4%의 순이었다. 8. 내막증의 병기와 임상증상 간에서 아무런 연관관계가 없었다. 9. 내막종의 경우에도 특징적인 임상증상이 없었으며, 다른 초기의 내막증의 증상과 차이가 없었다. 10. 자궁근종과 동반된 예는 15.4%였다. This is a clinical analysis of 65 cases of endometriosis diagnosed during laparotomies performed at the department of Obstetrics and Gynecology, School of Medicine, Keimyung University from Jan. 1986 to July 1988. The results are follow: 1. The incidence of endometriosis was 2.8 %, 65 cases among 2358 laparotomies. 2. The average age of the patients was 32.5 and the most frequent occurrence was noted in the group of thirties. 3. The symptoms comprised dysmenorrhea in 60.0 % of the case, pain just before menstruation in 30.9 %, dyspareunia in 29.2 %, and painful defication in 36.9 %. 4. Associated infertility combined with endometriosis was noted in 49.2 % of the cases. 5. The duration of infertility was less than 5 years in 68.8 % of the cases and the interval of diagnosis from the last pregnancy in non-infertility group was more than 6 years in 81.8 % of the cases. 6. The frequent pelvic findings comprised fixed mass at adnexa in 44.7 % of the case, nodular mass at uterosacral ligament in 26.2 %, and induration in cul-de-sac in 15.4 %. 7. The sites most frequently involved were ovary in 76.9 % of the cases and uterosacral ligament in 52.3 %. The other site were uterus, fallopian tubes, round ligament and bladder. 8. Clinical symptoms are no correlation with the extension of the disease. 9. Myoma was associated in 15.4 of cases.

포상기태 제거후 혈중 B-hCG값의 감소양상과 예후인자들

이태순(TS Lee),송기창(KC Song),김창이(CY Kim),배석년(SN Bae),정재근(JK Jung),김대훈(DH Kim),김승조(SJ Kim) 대한산부인과학회 1989 Obstetrics & Gynecology Science Vol.32 No.10

가톨릭의과대학 부설 융모성질환 연구소에 등록된 포상기태 환자 275예를 대상으로 혈중 B-hCG 경쾌기간에 따라 포상기태의 예후에 영향을 미치는 것으로 생각되는 인자들을 비교 분석하여 다음과 같은 결론은 얻었다. 1. 연령의 증가에 따라 혈중 B-hCG 경쾌기간은 증가하는 경향을 보였으나 통계적으로 유의는 없었다. 2. 기왕분만 횟수가 증가할수록 혈중 B-hCG 경쾌기간은 유의하게 길어졌다. 3. 부분 포상기태에서는 혈정 B-hCG 경쾌기간이 짧은 반면에 재발성 포상기태에서는 혈중 B-hCG 경쾌기간이 길었다. 4. 임신주수에 비하여 자궁의 크기가 클수록 혈중 B-hCG 경쾌기간은 길었다. 5. 포상기태 제거전 뇨중 hCG값이 높을수록 혈중 B-hCG 경쾌기간은 길었다. 6. 혈중 B-hCG 경쾌기간이 긴 경우에 존속성 융모성 빈도가 높았다. 이상의 결론으로 보아 혈중 B-hCG 경쾌기간이 길어질수록 예후가 나빴으며 고위험군에 대한 조기 화학요법 치료가 필요하다고 생각된다. 1. There appeared to be a tendency of increase is regressin period according to age, but not statistically significantly 2. Regression period became prolonged with increased number of parity 3. There was an incarese in regression period in complete mole group and a sincindence increase in regression period in recurrent mole group when compared with that of partial mole group . 4. In relation of uterine size to gestatinal months there was significantly increase in regression period in large for dates group when compared with that of normal and small for dates group 5. There was significantly increase in regression period in the higher initial hCG titer group than the lower group 6. frequency of persistnat gestational trophoblastic tumor was higher with those who showed prologed B-hCG regression period.

남성화배세포종의 1례

송태섭(TS Song),위흥선(HS Wui),이종수(JS Lee),이주석(JS Lee),김상태(ST Kim) 대한산부인과학회 1972 Obstetrics & Gynecology Science Vol.15 No.5

1) 남성화배세포종의 1예를 75세의 고령부인에서 발생했음을 보고한다. 2) 조직학적으로 중간형임을 알았다. 3) 본증례에서는 임상적으로 명백한 남성화 증상과 적출후의 특별한 증상변화등은 없었다. A 75-year-old Korean woman, para 4, whose menopause had occured at 50, was admitted to Sacred Heart Hospital on May 25, 1970, because of progressive lower abdominal distension, child head sized palpable. mass, frequent urination, constipation, rough deeper as male voice and lower abdominal discomfortness of two months duration. On the admission, her pulse rate was 98; her blood pressure, 160/105; and respiration, 28 the lungs were clear with decreased sounds in both bases. Heart sounds were normal. There was an atrophic, clean cervix with a small uterus bushed laterally by a child head sized, cystic, smooth mass occupying the entire pelvis and lower abdomen which reached just below the umbilicus but clitoris was not so atrophic inspite of her age. Marked rough and deeper as male voice and atrophic breasts were evident. The hematocrit was 38, and the white blood cell count was normal. The patient`s urinalysis, liver function test, and biochemical profile were also normal. A lower median incision was made, and numerous fine adhesion between the parietal peritoneum and the cyst wall were freed easily by blunt dissection. The cyst was found to arise from the right ovary with the descending colon and ileum as well as the right uterus firmly adherent to it. The left ovary was atrophic. An egg sized round, hard mass was found on the mid ileum which should be metastasized from the ovary. The cyst was then removed with some difficulty, and a radical hysterectomy and segmental resection of ileum were performed. Post operatively, the patient did well except for one episode of urticarial reaction which lasting for a few hours. The patient left the hospital in good health on the 20th post operative day. The ovarian tumor had central necrosis. Histologic sections revealed the ovarian cyst and a tumor of ileum to be a intermediate type of arrhenoblastoma.

Exogenous DNA Uptake of Boar Spermatozoa by a Magnetic Nanoparticle Vector System

Kim, TS,Lee, SH,Gang, GT,Lee, YS,Kim, SU,Koo, DB,Shin, MY,Park, CK,Lee, DS Blackwell Publishing Ltd 2010 REPRODUCTION IN DOMESTIC ANIMALS Vol.45 No.5

<P>Contents</P><P>The sperm-mediated gene transfer method is applicable to transgenesis in many species that use spermatozoa for reproduction recently, which has been shown various results. In the current study, we show that transgenic porcine embryos can be efficiently produced by employing a simple transfection method that uses magnetic nanoparticles (MNPs). The complexes formed between plasmid DNA and MNPs were bounded on ejaculated boar spermatozoa at a higher efficiency compared to methods using DNA alone or lipofection. Using confocal microscopy, rhodamine fluorophore-labelled MNPs were detected on external surfaces of the spermatozoa membrane, which were bounded on zona pellucida of <I>in vitro</I> maturated oocyte during <I>in vitro</I> fertilization. Electron microscopy revealed that clusters of MNPs were detected in inside of plasma membrane and nucleus of the spermatozoa head. Additionally, we found that magnetofected boar spermatozoa could be fertilized with oocytes <I>in vitro</I> and that the resulting gene of green fluorescent protein was detected in fertilized eggs by genomic PCR analysis. Taken together, these results suggest that MNPs can be used to efficiently introduce a transgene into embryo via spermatozoa.</P>