Seroprevalence of Toxoplasma gondii and Trichinella spiralis infections in wild boars (Sus scrofa) in Korea

Kang, S.W.,Doan, H.T.T.,Noh, J.H.,Choe, S.E.,Yoo, M.S.,Kim, Y.H.,Reddy, K.E.,Nguyen, T.T.D.,Van Quyen, D.,Nguyen, L.T.K.,Kweon, C.H.,Jung, S.C. Elsevier 2013 Parasitology international Vol.62 No.6

Toxoplasma gondii and Trichinella spiralis are important zoonotic pathogens with worldwide distributions. In Korea, several outbreaks of human toxoplasmosis and trichinellosis due to the consumption of infected wild animals have been reported. The purpose of this study was to determine the seroprevalence of T. gondii and T. spiralis infections in wild boars killed in Korea from December 2009 to October 2011. A total of 521 wild boars hunted in eight provinces were examined for antibodies to T. gondii and T. spiralis by using commercial ELISA kits. Overall, 25.1% of serum samples from individual boars were seropositive for T. gondii and 1.7% were seropositive for T. spiralis. Seropositive for T. gondii was found in the boars in all the eight provinces investigated and for T. spiralis in four provinces. This is the first report on the seroprevalence of T. gondii and T. spiralis infections in wild boars in Korea. The consumption of undercooked wild boar meat may expose humans to a high risk of infection.

채종박 품질이 이유돈의 성장에 미치는 영향

강창원,남기택,임호중,공진훈,안상태 한국축산학회 1995 한국축산학회지 Vol.37 No.4

This study was made to evaluate the nutritional values and glucosinolate contents of three types of rapeseed oil meal(RSM), originated from Canada(canola), China(CRSM:Chinese Rapeseed Oil Meal) and India (IRSM:Indian Rapeseed Oil Meal), and their dietary effects on the performance of weanling pigs. Nutritional evaluation included the contents of moisture, crude protein(CP), ether extracts (EE), crude fiber(CF), Ash, Ca and P, KOH solubility, tannin, and glucosinolate contents of each RSM. Total of two hundred-forty weanling pigs weighing about 20㎏ initially were housed in 24 pens with 10 pigs per pen. The thirty animals were assigned to one of eight rations : T₁ (0% RSM + 2% molasses), T₂ (6% canola + 2% molasses), T₃ (6% CRSM + 2% Molasses), T₄(6% IRSM + 2% molasses), T_5 (0% RSM + 5% molasses), T_6 (6% Canola + 5% molasses). T_7 (6% CRSM + 5% molasses), T_8 (6% IRSM + 5% molasses). During the experimental period, body weight and feed intakes were measured weekly and feed conversion rates were calculated. The results obtained from this experiments are summarized as follows. 1. The KOH solubility of canola, CRSM and IRSM was found to be 54.6, 35.7 and 64.8%, respectively. The glucosinolate contents of canola, CRSM and IRSM were found to be 0.11, 0.22 and 0.79%, respectively. 2. There were no significant differences between the 2% molasses and the 5% molasses supplemented groups in feed intake and body weight gain. 3. The 6% CRSM(T₃, T_7) and IRSM(T₄, T_8) groups were significantly (P$lt;0.05) inferior to the control groups(T₁, T_5) and 6% canola groups(T₂, T_6) in feed intake and body weight gains. The control groups(T₂, T_6) were significantly (P$lt;0.05) superior to the 6% canola groups(T₂, T_6), 6% CRSM(T₃, T_7) and 6% IRSM groups(T₄, T_8) in feed conversion rate.

Deletion in HSP110 T₁₇: correlation with wild-type HSP110 expression and prognostic significance in microsatellite-unstable advanced gastric cancers

Kim, K.J.,Lee, T.H.,Kim, J.H.,Cho, N.Y.,Kim, W.H.,Kang, G.H. W. B. Saunders Co ; Centrum Philadelphia 2017 Human pathology Vol.67 No.-

<P>Deletion of the HSP110 T-17 mononucleotide repeat has recently been identified as a prognostic marker that is correlated with wild-type HSP110 (HSP110wt) expression in microsatellite instability-high (MSI-H) colorectal cancers. The aim of this study was to assess the correlation between deletion of the HSP110 T-17 repeat and expression of HSP110wt using DNA testing and immunohistochemistry and to determine the prognostic implications of HSP110 T-17 deletion in MSI-H advanced gastric cancers (GCs). The status of HSP110wt expression was evaluated by immunohistochemistry using an HSP110wt-specific antibody in 142 MSI-H advanced GCs. The size of the HSP110 T-17 repeat deletion was analyzed in 96 MSI-H advanced GCs; deletions were divided into small (0-2 base pairs) and large deletions (3-5 base pairs). Low and high expressions of HSP110wt were detected in 38 (26.8%) and 104 (73.2%) of the 142 cases, respectively. The HSP110 T-17 deletion was observed in 45 (46.9%) of the 96 MSI-H GC samples. Tumors with high expression of HSP110wt showed a tendency to have small or no deletion of HSP110 T-17. In Kaplan-Meier survival analysis, tumors with a large HSP110 T-17 deletion were associated with favorable overall survival and disease-free survival compared with those with small/no deletion of HSP110 T-17. However, HSP110 T-17 deletion size was not an independent prognostic factor in multivariate analysis. In summary, deletion of the HSP110 T-17 repeat was frequently observed in MSI-H GCs, and HSP110 T-17 deletion size was inversely correlated with HSP110wt expression status. Large HSP110 T-17 was not a prognostic indicator in MSI-H GCs. (C) 2017 Elsevier Inc. All rights reserved.</P>

육계사료와 식용유 정제 폐백토(Spent Bleaching Clay) 급여가 육계의 생산성에 미치는 영향

전해열,손장호,이길왕,김선구,강한석,신택순,조병욱,Jeon H. Y.,Son J. H.,Lee K. W.,Kim S. K.,Kang H. S.,Shin T. S.,Cho B. W. 한국가금학회 2005 韓國家禽學會誌 Vol.32 No.4

본 연구는 Spent Bleaching Clay(SBC)를 육계사료의 에너지 공급원으로 활용 가능성을 구명하기 위하여 실시하였다. 부화 직후의 120수의 병아리(한협 3호)를4처리 3반복, 반복당 10수(암수 각각 5수씩)를 Control(T1) 사료에 SBC를 $2.0\%(T2),\;4.0\%(T3)$ 및 $6.0\%(T4)$ 첨가한 4처리로 배치하였다. 시험사료 급여전 1주일은 시판사료를 급여하였다. 4주간의 시험 기간동안 증체중 및 사료 섭취량은 T3 및 T4 처리구에서 증가하는 경향이 인정되었다. 사료효율은 T3구에서 개선되는 경향이 인정되었다. 가슴살 및 다리살에서 치방산 조성을 사료의 처리에 따른 효과는 인정되지 않았다. 육계 초기 및 후기 사료 급여시 모두, 사료중의 건물, 조단백질 및 조지방 이용율은 T4 처리구가 다른 처리구에 비해서 유의하게 감소하였으며(p<0.05), 대사에너지가도 T4 처리구가 다른 3구에 비해서 유의하게 저하하였다(p<0.05). 사료중의 조회분의 이용율은 사료의 처리에 따른 차이는 인정되지 않았다. 결론적으로 본 시험의 결과 육계사료에 $2\~4\%$의 SBC첨가는 육계의 성장 및 사료효율 개선의 가능성이 있음이 사료된다. This study was conducted to evaluate the feasibility of spent bleaching clay(SBC) as an energy resource for meat type chicks. A total of one hundred twenty 1-d old broiler chicks(Han-hyop, No. 3) were divided into four groups and each group was assigned to one of the following diets: 0(T1), 2.0(T2), 4.0(T3) and $6.0\%$ SBC(T4). Each treatment had 3 replications of 10 birds. Before feeding experimental diets, they were fed a commercial diet for a week Body weight gain and feed intake tended to increase in T3 and T4, respectively. Feed efficiency(feed intake/ gain) tended to decrease in T3 compared to other groups. Fatty acid composition of breast and thigh meat was not significantly affected by feeding SBC. Utilization of dry matter, energy, crude protein and crude fat was lower in T4 than in the other groups during both starter and finisher periods(p<0.05). Crude ash utilization was not significantly affected by feeding dietary SBC. These results indicate that broiler diets may be supplemented with 2 to $4\%$ SBC without adverse effect on growth.

Retinoid-mediated inhibition of interleukin-12 production in mouse macrophages suppresses Th1 cytokine profile in CD4+ T cells

Kang, B . Y .,Chung, S . W .,Kim, S.H,Kang, S . N .,Choe, Y . K .,Kim, T . S . 전남대학교 약품개발연구소 2000 약품개발연구지 Vol.9 No.1

1 Interleukin-12 (IL-12) plays a central role in the immune system by driving the immune response towards T helper I (Th1) type responses characterized by high IFN-γ and low IL-4 production. In this study we investigated whether retinoid-mediated inhibition of interleukin-12 production in mouse macrophages could regulate cytokinc profile of antigen (Ag)-primed CD4^+ Th veils. 2 Pretreatment with retinoids (9-cis-RA, all-trans-RA, TTNPB) significantly inhibited IL-12 production by mouse macrophages stimulated with lipopodlysaccharide (LPS) or heated-killed Lisreria monocytogenes (HKL). Retinoid-pretreated macrophages reduced their ability to induce IFN-γ and increased the ability to induce IL-4 in Ag-primed CD4^+ T cells. 3 Addition of recombinant IL-12 to cultures of retinoid-pretreated macrophages and CD4^+ T cells restored IFN-γ production in CD4^+ T cells. 4 The in vivo administration of 9-cis-RA resulted in the inhibition of IL-12 production by macrophages stimulated in vivo with either LPS or HKL, lending to the inhibition of Th1 cytokine profile (decreased IFN-γ and increased IL-4 production) in CD4^+ T cells. 5 These findings may explain some known effects of retinoids including the inhibition of encephalitogenicity, and point to a possible therapeutic use of retinoids in the Th1-mediated immune diseases such as autoimmune diseases.

사료급여방법이 제주재래마의 체성장 및 도체품질에 미치는 영향

오운용(W . Y . Oh),강태홍(T . H . Kang),김동철(D . C . Kim),진신흠(S . H . Jin),홍성귀(S . K . Hong),양승주(S . J . Yang),정재홍(J . H . Jung) 한국축산학회 1993 한국축산학회지 Vol.35 No.6

Nine Cheju Native Horses with an average weight of 169.2㎏ were used in a 238-d (34-wk) fattening trial designed to evaluate the effects of feeding method on growth performance and carcass characteristics. Horses were randomly alloted to three treatment groups: T₁ 6-wk (Former fattening period: FFP) + 28-wk (Latter fattening period: LFP), T₂, 9-wk (FFP) + 25-wk (LFP), T₃, 12-wk (FFP) + 22-wk (LFP). During the FFP, concentrate was restricted at approximately l.5% of their body weights but horses were allowed ad libitum access to concentrate during the subsequent LFP. The horses were individually penned in 2.5-m×3-m pipe stalls with front feeding gates. They were allowed 3 h of exercise in a pen (50-m×30-m) outside the barn monthly. Horses were weighed at 30-d intervals and feed consumption was measured daily. At the end of the 238-d fattening period, one horse in each treatment was selected randomly and slaughtered for meat quality evaluation. Results obtained from the present study were summarized as follows; during the overall fattening period, average daily gains (ADG) and nutrient intake (TDN) per ㎏ wt, gain were 0.59, 5.10(T₁), 0.51, 5.84(T₂) and 0.43, 6.37㎏(T₂), respectively. When horses were allowed ad libitum access to concentrate for 28 wk(T₁), ADG and feed efficiency(TDN) were improved by 37%(P$gt;.05) and20%(P$gt;.05)compared to those allowed concentrate free choice for 22 wk(T₃). Horse in T, group had an improved dressing percentage and carcass weight with good quality grades(intramuscular fat, meat color, fat color). Taste panelists(150 amateures) detected no difference from longissimus lumborum steaks between Cheju Native Horse and improved Cheju Native Cattle, but found juiciness and meat flavor were superior to those of beef.

농촌형 녹색마을에 신재생에너지 보급을 위한 시설재배 및 농업기계의 CO2 배출량 분석

김종구(J.G. Kim),유영선(Y.S. Ryou),강연구(Y.K. Kang),김영화(Y.H. Kim),장재경(J.K. Jang),김현태(H.T. Kim),서광욱(K.W. Seo),이승기(S.K. Lee),조희제(H.J. Cho),강지원(J.W. Kang) 유기성자원학회 2011 유기물자원화 Vol.19 No.1

화석에너지 의존도를 줄이면서 CO2 배출량을 낮추기 위하여 정부에서는 녹색마을을 선정하고 에너지자급률을 40% 수준으로 높이려는 계획을 추진 중이다. 본 연구는 각 농업 분야 중에서 농기계의 사용과 재배 시설에 있어서의 에너지 사용량을 파악하고 이를 바이오디젤로 대체하였을 때의 CO2 저감수준을 분석하고자 하였다. 이를 위하여, 농업 각 분야별 에너지 소비수준의 분석, 그리고 실천 가능한 신재생 에너지원의 선정이 요구된다. 경종재배의 전체 연간온실가스 배출량은 5,667,258 t-CO2이고, 그 중 시설 부문은 4,932,607 t-CO2인 것으로 분석되었으며, 농업시설 부문 중 에너지원별로 보면 경유가 3,105,707t-CO2, 중유가 1,370,578 t-CO2를 배출하는 것으로 분석되었다. 우리나라 시설작물의 단위 면적당 온실가스 평균배출량은 29,418 t-CO2/ha인 것으로 나타났다. 농기계별 2007년 총에너지소비량을 살펴보면 트랙터가 284,763 kL로 가장 높게 나타났으며, 동력 경운기221,314 kL, 곡물건조기 145,524 kL, 콤바인 72,537 kL 등의 순이었다. 전라북도 G시를 대상으로 이용 중인 시설재배와 농업기계의 이산화탄소 배출량을 비교분석한 결과,바이오디젤로 전환하면 약 7% 정도의 CO2 감소효과가 있는 것으로 분석되었다. In order to reduce dependence on the fossil fuels and CO2 gas emission in farming activities, the government has pushed ahead with making the self-sufficiency of farming energy up 40% level in green villages. The objectives of this study are to survey the energy consumption of horticultural facilities or agricultural machineries, and to analyze the reduced CO2 gas emission level from fossil fuel to bio-diesel fuel. For the implement of this study, it is necessary to analyze the energy consumption level in the various sector of farming activities, and available renewable energy sources should be selected. Annual total CO2 gas emission in the tillage farming sector was analyzed as 5,667,258 t-CO2 and that in the horticultural facilities occupied 4,932,607 t-CO2, while the CO2 gas emission level of diesel fuel was 3,105,707 t-CO2, and that of the heavy oil showed 1,370,578 t-CO2. The average CO2 gas emission level of horticultural facilities in the country was analyzed as 29,418 t-CO2/ha. Among the total energy consumption of agricultural machineries, tractor used 284,763kL, power tiller spent 221,314 kL, grain drier consumed 145,524kL and combine tractor expend 72,537kL. From the comparison of CO2 gas emission level between fossil fuel and bio-diesel fuel for the horticultural facilities or agricultural machinery in G-City, Jeonbuk Province, the CO2 gas emission level can be reduced by 7% through replacing the fuel from fossil to biodiesel.

Nasal-type NK/T-cell lymphomas are more frequently T rather than NK lineage based on T-cell receptor gene, RNA, and protein studies: lineage does not predict clinical behavior

Hong, M.,Lee, T.,Young Kang, S.,Kim, S. J.,Kim, W.,Ko, Y. H. Nature Publishing Group 2016 Modern pathology Vol.29 No.5

<P>Extranodal natural killer (NK)/T-cell lymphoma (ENKTL), nasal type, comprises NK or cytotoxic T cells. We evaluated the clinical impact of cell type and the usefulness of T-cell receptor (TCR) gene transcripts in distinguishing cell lineage. One hundred and eight cases of ENKTL were analyzed for TCR gene rearrangements using the BIOMED-2 protocol and for TCR gene expression using immunohistochemistry for TCR-beta F1 and TCR-c gamma M1, and RNA in situ hybridization for TCR gene transcripts. Prognostic factors were analyzed. Among the 108 cases, 44 were monoclonal for a TCR rearrangement (40%) while 64 (60%) were undefinable. The monoclonal cases expressed TCR-beta F1 in 14 out of 40 cases (35%) and TCR-c gamma M1 in 1 out of 44 cases (2%). The 64 undetermined cases expressed TCR-beta F1 in 15 cases (23%) and TCR-c gamma M1 in 1 (2%). Thirteen of 40 TCR-beta constant gene transcript-positive cases (33%) expressed TCR-beta F1 and one of nine TCR-gamma constant gene transcript-positive cases (11%) expressed TCR-c gamma M1. TCR gene transcripts were not useful in the distinction of cell lineages. TCR gene transcripts were positive in ENKTLs as well as in normal B cells and aggressive NK-cell leukemia. Based on gene rearrangements and immunohistochemistry for TCR, there were 60 T-cell type cases (56%), 32 NK-cell type cases (30%), and 16 cases with an undetermined cell type (14%). TCR protein was expressed in 30/60 T-ENKTLs (50%) in a variable fraction of tumor cells. There were no significant differences in clinical findings or overall patient survival between T-or NK-cell types of ENKTL, although those with a T-cell type tended to show a better prognosis for those with localized nasal lymphomas. Univariate and multivariate analysis showed that a non-nasal ENKTL, age 460 years, high level of lactate dehydrogenase, bone marrow involvement, and the absence of radiotherapy were independent prognostic factors.</P>

육계분 발효사료의 첨가수준이 숫꽃사슴의 소화율, 건물채식량 및 질소출납에 미치는 영향

전병태,곽완섭,강성기,이상무,문상호 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.1

본 실험은 기존 사슴사료에 있어서 육계분 발효사료의 대체 가능성을 검토하기 위해 숫사슴에 있어서 육계분 발효사료의 첨가수준을 0, 15 및 30%로 달리하여 소화율, 증체량, 건물채식량 및 질소출납에 미치는 영향을 검토하였다. 모든 처리구에서 실험사슴들은 비슷한 수준의 채식량을 나타내어 건물기준으로 체중의 약 3% 정도의 채식량을 기록하고 있어 육계분 발효사료를 30%까지 첨가한 경우 숫사슴의 채식 기호도에는 부정적인 영향이 나타나지 않는 것으로 판단되었다. 건물소화율은 Control이 76.5%로 가장 높았고, T2가 70.3%로 가장 낮아 유의성(P<0.05)이 인정되었다. 조단백질의 경우도 Control구가 75.8%로 가장 높은 소화율을 나타내었으며, T2가 70.2%로 가장 낮은 소화율을 나타내어 처리간에 유의성(P<0.05)이 인정되었다. 조섬유 소화율은 Contol구 70.8%, T1구 62.7%, T2구 56.0%로 육계분 발효사료의 첨가수준이 높아짐에 따라 조섬유 소화율이 저하되어 Control구와의 사이에 유의성(P<0.05)이 인정되었다. 건물 및 가소화 건물섭취량 모두 육계분 발효사료의 첨가수준이 높아질수록 약간씩 저하되는 경향은 있었으나 그 차이에 대한 유의성은 인정되지 않았다. 실험사슴들의 일당 증체량은 T1구에서 가장 높았으며 Control구에서 가장 낮았으나 처리간의 차이는 유의성이 인정되지 않았다. 질소섭취량은 T1구가 40.2g으로 가장 많았으며 T2구가 38.0g으로 가장 낮았으나 처리간의 유의성은 인정되지 않았다. 분 중 질소의 양은 T2구에서 가장 높았으며 뇨 중 질소의 양은 Control구가 가장 높았다(P<0.05). 체내 질소 축적량은 T1구에서 가장 높았으나 나머지 처리구들과 비슷한 수준을 유지했다. To determine nutritive value and suitability of deep-stacked broiler litter for deer, influence of supplementary levels of broiler litter on digestibility, dry matter intake, and nitrogen balance was investigated in male spotted deer (Cervus nippon) fed a commercial mixed(complete) ration. The deep-stacked and ground broiler litter was supplemented at the level of 0%(Control). 15%(TI) and 30%(T2) on the basis of fresh matter to the mixed ration. All treatments had similar palatability at about 3% body weigh of dry matter intake. Dry matter digestibility was highest in Control and lowest in T2(P<0.05). Digestibility of crude protein was lowest(P<0.05) in T2. Dry matter and digestible dry matter intake was similar in all treatments. Daily body weight gain was not affected by treatments. Feeding 15 or 30% of broiler litter to male deer resulted in similar nitrogen intake. fecal nitrogen excretion and daily nitrogen retention(g/d), compared with feeding Control. These results suggest that up to 30% of broiler litter may be fed to male deer without deleterious effects on deer performance.

Roseovarius halotolerans sp. nov., isolated from deep seawater

Oh, Y.-S.,Lim, H.-J.,Cha, I.-T.,Im, W.-T.,Yoo, J.-S.,Kang, U. G.,Rhee, S.-K.,Roh, D.-H. Microbiology Society 2009 International journal of systematic and evolutiona Vol.59 No.11

<P>A Gram-reaction-negative, non-motile, aerobic bacterium, designated HJ50(T), was isolated from deep seawater of the East Sea, South Korea. Cells were ovoid to rod-shaped (0.5-0.8x1.3-3.0 mum), often with unequal ends, suggesting a budding mode of reproduction. The strain had an absolute requirement for sea salts and tolerated up to 20 % (w/v) sea salts. Propionate, dl-lactate, 2-ketogluconate, 3-hydroxybutyrate and rhamnose were used as growth substrates, but not mannitol, salicin, 4-hydroxybenzoate or acetate. The major fatty acid was summed feature 7 (C(18 : 1)omega7c/omega9t/omega12t) and the DNA G+C content was 59.0+/-0.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that this strain was affiliated with the genus Roseovarius. Similarities between the 16S rRNA gene sequence of strain HJ50(T) (1430 nt) and those of type strains of members of the genus Roseovarius were 94.1-96.3 %. DNA-DNA relatedness values between strain HJ50(T) and the type strains of members of the genus Roseovarius were low (1.3-24.6 %). Physiological and biochemical differences support assignment of strain HJ50(T) to the genus Roseovarius as a representative of a novel species. The name Roseovarius halotolerans sp. nov. is proposed, with HJ50(T) (=KCTC 22224(T) =LMG 24468(T)) as the type strain.</P>