In Vitro Probiotic Properties of Indigenous Dadih Lactic Acid Bacteria

Surono, Ingrid S. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.5

The aim of this research was to identify candidate probiotic lactic bacteria among indigenous dadih lactic isolates. Dadih is an Indonesian traditional fermented milk of West Sumatra which is fermented naturally. Viability of the strain is critical in determining the capacity of lactic bacteria to induce immune stimulation as well as to colonize in the intestinal tract. Therefore, LAB are proposed to exert health promoting or probiotic effects in human, such as inhibition of pathogenic microflora, antimutagenic, and the reduction of cholesterol levels. This manuscript reports in vitro probiotic properties of indigenous dadih lactic bacteria, especially some important colonization factors in GI tract, such as lysozyme, acid and bile tolerance. Bile Salt Hydrolase (BSH) activity, spectrum of bacteriocin, and antimutagenic activity of bacterial cells were also assessed. Twenty dadih lactic isolates were screened further for their tolerance to low pH, at pH 2 and 3 as well as their bile tolerance. There were ten isolates classified as acid and bile acid tolerant, and further screened for lysozyme tolerance, BSH activity. The spectrum of bacteriocin activity of isolates was assayed using cell-free neutralized supernatants by agar spot test against variety of pathogens. Lc. lactis subsp. lactis IS-10285, IS-7386, IS-16183, IS-11857 and IS-29862, L. brevis IS-27560, IS-26958 and IS-23427, Leu.mesen.mesenteroides IS-27526, and L. casei IS-7257 each has good survival rate at low pH values and in the presence of lysozyme, and short lag time in the presence of 0.3 % oxgall. Lc. lactis subsp. lactis IS-11857 and IS-29862 each has high BHS activity, Lc. lactis subsp. lactis IS-10285 and IS-16183 each had a positive spectrum of bacteriocin activity against E. coli 3301 and Lysteria monocytogenes ATCC 19112, while L. brevis IS-26958 has high BHS activity as well as positive spectrum of bacteriocin against E. coli 3301, Lysteria monocytogenes ATCC 19112, and S. aureus IFO 3060. All of the ten dadih lactic strains performed in vitro acid and bile tolerance, indicating a possibility to reach the intestine alive, and display probiotic activities.

In vivo Antimutagenicity of Dadih Probiotic Bacteria towards Trp-P1

Surono, Ingrid S.,Pato, Usman,Koesnandar, Koesnandar,Hosono, A. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.1

In vitro acid- and bile-tolerant lactic acid bacteria isolated and identified from Indonesian traditional fermented milk dadih might be considered as potential probiotic strains after further characterization with animal models, especially for their therapeutic properties. Five dadih lactic bacteria isolates each had moderate survival rate for 2 h at pH 2.0, as well as bile tolerance. The aim of this research was to identify candidate probiotic lactic bacteria among indigenous dadih lactic isolates originated from Bukit Tinggi, West Sumatra, especially their in vivo antimutagenic property. Milk cultured with Enterococcus faecium IS-27526 significantly lowered fecal mutagenicity of rats as compared to the control group, skim milk, and milk cultured with L. plantarum IS-20506. These results suggest that Enterococcus faecium IS-27526 may serve as a potential probiotic strain with its antimutagenicity.

Adhesion Properties of Indigenous Dadih Lactic Acid Bacteria on Human Intestinal Mucosal Surface

Dharmawan, Jorry,Surono, Ingrid S.,Kun, Lee Yuan Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.5

Dadih is Indonesian traditional fermented buffalo milk believed by the natives to have beneficial effects on human health. This may be due to the probiotic properties possessed by the lactic acid bacteria (LAB) involved in its fermentation process. It was discovered that ten strains of dadih lactic isolates possessed some probiotic properties in vitro. In this study, the adhesion properties of dadih LAB, in comparison with documented probiotic strains, were investigated in vitro by using mucin extracted from human faeces and Caco-2 cells as the models for human intestinal mucosal surface and intestinal cells respectively. The adhesion results showed the distinction of Lactobacillus reuteri IS-27560 in adhering to both mucus layer and Caco-2 cells. The competition assay for adhesion to the mucus layer between dadih LAB and selected pathogens indicated the competence of Lactococcus lactis IS-16183 and Lactobacillus rhamnosus IS-7257 in significantly inhibiting the adhesion of Escherichia coli O157:H7. Accordingly, these two strains may be potential candidates for use as probiotic strains. Overall, the adhesion properties of all dadih LAB strains were relatively comparable to that of Lactobacillus casei Shirota and Lactobacillus rhamnosus GG, the documented probiotic strains.

Hypocholesterolemic Effect of Indigenous Dadih Lactic Acid Bacteria by Deconjugation of Bile Salts

Pato, Usman,Surono, Ingrid S.,Koesnandar, Koesnandar,Hosono, Akiyoshi Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.12

Administration of milk and fermented milks produced from indigenous dadih lactic acid bacteria on serum lipids and bile acids, fecal bile acids and microflora was estimated in hypercholesterolemic rats. Anaerobic lactic acid bacteria decreased and coliforms increased in the feces of the control group; however, the number of fecal lactic acid bacteria remained unchanged when rats were administered milk and fermented milks. Only fermented milk made from Lc. lactis subsp. lactis IS-10285 significantly reduced serum total cholesterol, LDL cholesterol and total bile acids. Milk and fermented milks did not influence the HDL cholesterol. Triglyceride and phospholipid levels were significantly lower in the rats fed fermented milk of Lc. lactis subsp. lactis IS-10285 than rats fed milk and fermented milk of Lc. lactis subsp. lactis IS-29862, but not significantly different from the control group. Hypocholesterolemic effect of Lc. lactis subsp. lactis IS-10285 was attributed to its ability to suppress the reabsorption of bile acids into the enterohepatic circulation and to enhance the excretion of bile acids in feces of hypercholesterolemic rats.

Implementation of Rapid Application Development Method in the Development of Geographic Information Systems of Industrial Centers

Sasmito, Ginanjar Wiro,Wibowo, Dega Surono,Dairoh, Dairoh The Korea Institute of Information and Commucation 2020 Journal of information and communication convergen Vol.18 No.3

Cities in Indonesia include diverse scattered industrial centers comprising industries that can sustain the existing economic pace. For industrial data collection, the city government still relies on census that consumes extensive time and money. The public are unfamiliar with industries owing to their lack of industry information; therefore, the market share is not optimal. In addition, the opportunity to procure investors for business development is limited. A Geographic Information System (GIS) is a computer system that can record, store, write, analyze, and display geographical data. Using the Rapid Application Development (RAD) method, GIS was developed on a website platform to provide information on industry profiles, types of production, investment values, industry maps, and industrial locations in each village and sub-district to a wider community. The RAD method was chosen compared to the waterfall method because it could accelerate website development process.

Improving the success of direct seeding through the application of seed briquettes, aquasorb, and sowing time: case studies on Ceiba pentandra, Enterolobium cyclocarpum, and Calophyllum inophyllum

Dede J. Sudrajata,Evayusvita Rustam,Nurhasybi,Nurin Widyani,Yulianti,Yupi Isnaini,Popi Aprilianti,Enggal Primananda,Muhammad Zanzibar,Suhartati,Kurniawati P. Putri,Naning Yuniarti,Surono,Vivi 한국산림과학회 2023 Forest Science And Technology Vol.19 No.2

Direct seeding, a planting technique that has long been applied in restoring degraded land and forest, is often doubted to be successful due to many constrains, both biotic and abi- otic. This study aims to increase the success of direct seeding through the application of seed briquettes, aquasorb treatment and determining the right sowing time for three forest tree species, i.e. Ceiba pentandra, Enterolobium cyclocarpum, Calophyllum inophyllum.This study used a randomized block design with two factorials (seed treatments and sowing dates) in each species. Seeds were sown in three blocks in the field according to the sowing time treatments. Each treatment consisted of 10 sowing plots in one block and 5 seeds or seed briquettes in each sowing plot. The results showed that direct seeding when the rain starts to stabilize (ST-2) supported by the use of seed briquettes and the addition of aqua- sorb was able to increase seedling survival, height and root collar diameter of all tested tree species. In general, seed briquettes and the addition of aquasorb provided better seedling survival and growth compared to direct seeding using untreated seeds. C. inophyllum gave higher seedling survival compared to the other two species indicating that this species is very prospective for direct seeding applications due to its high adaptability.