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- 저자 : Yupi Isnaini (검색결과 2 건)
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Dede J. Sudrajata,Evayusvita Rustam,Nurhasybi,Nurin Widyani,Yulianti,Yupi Isnaini,Popi Aprilianti,Enggal Primananda,Muhammad Zanzibar,Suhartati,Kurniawati P. Putri,Naning Yuniarti,Surono,Vivi 한국산림과학회 2023 Forest Science And Technology Vol.19 No.2
Direct seeding, a planting technique that has long been applied in restoring degraded land and forest, is often doubted to be successful due to many constrains, both biotic and abi- otic. This study aims to increase the success of direct seeding through the application of seed briquettes, aquasorb treatment and determining the right sowing time for three forest tree species, i.e. Ceiba pentandra, Enterolobium cyclocarpum, Calophyllum inophyllum.This study used a randomized block design with two factorials (seed treatments and sowing dates) in each species. Seeds were sown in three blocks in the field according to the sowing time treatments. Each treatment consisted of 10 sowing plots in one block and 5 seeds or seed briquettes in each sowing plot. The results showed that direct seeding when the rain starts to stabilize (ST-2) supported by the use of seed briquettes and the addition of aqua- sorb was able to increase seedling survival, height and root collar diameter of all tested tree species. In general, seed briquettes and the addition of aquasorb provided better seedling survival and growth compared to direct seeding using untreated seeds. C. inophyllum gave higher seedling survival compared to the other two species indicating that this species is very prospective for direct seeding applications due to its high adaptability.
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Modification of In Vitro Culture Method of Paphiopedilum glaucophyllum for Callus Induction
Elizabeth Handini,Ratna Uli Damayanti Sianturi,Popi Aprilianti,Yupi Isnaini,Endang Semiarti,Sri Rianawati,Saniyatun Mar’atus Solihah 한국육종학회 2023 Plant Breeding and Biotechnology Vol.11 No.4
The in vitro vegetative propagation technique of the Paphiopedilum glaucophyllum (callus propagation) still haveproblems to date. The aim of this study is to determine the effect of the media and plant growth regulator (PGR) on seed germination andcallus induction with shoot tip as an explant and use the planlet from elongation treatment result. The seed germination uses modifiedKnudson C (KC medium) with the addition of 3 concentrations of NAA (0; 5; 10 mg/L). The second study is the elongation treatmentof the explants uses four modified Murashige and Skoog (MS) medium (1/2 P, ½ P5, MP and MP5). And for the callus induction studyconsisted of two methods, callus induction with direct planting to the treatment medium by using SH medium with addition of2,4-Dichlorophenoxyacetic acid (2,4-D) 1 mg/L and Thidiazuron (TDZ) (0, 0.5, 1, 1.5 dan 2 mg/L). The second method used theexplants resulted from elongation treatment. It used half-strength Wattanawikkit medium with addition of TDZ (0; 0.5; 1; 1.5; 2 mg/L)and 2,4-D (0; 1; 5; 10 mg/L). All callus then subcultured in the half-strength MS (½ MS) medium containing 2,4-D 1 mg/L and TDZ (0;1; 1.5 mg/L). All the experiments used a completely randomized design with 3 repetitions. The results showed that the seeds germinated2 months after planting (MAP) for all media. The optimal media for explants elongation of P. glaucophyllum was MP5 media. Meanwhile, for the first callus induction experiments, explants had more callus in basic SH media with 2.4-D 1 mg/L and TDZ 0.5-1.5mg/L that grew in the dark. The second experiment, the optimal medium for callus induction was half MS with Thidiazuron 1.5 mg/Land 2.4-D 1 mg/L (T1.5D1). The the suitable medium for callus development is ½ MS) medium with 2,4-D 1 mg/L.
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