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Seunghyun Lee,Yerim Lee,Seunghwan Lee 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
The notorious lamiine species Asian Longhorn Beetle, Anoplophora glabripennis, is one of the major invasive pests of street trees, which is an endemic species of Korea and China. By far, the distributional range of Korean A. glabripennis population is restricted to only central to northeastern forest region of South Korea. In this study, we found unreported A. glabripennis populations in 4 different port cities and adjacent cities. These new populations are found in very much urbanic regions; Incheon, western part of Seoul, Busan and Ulsan. These populations can be distinctly differentiated with previously reported populations by massive damage on trees, unusual usage of host plant and spotted elytral pattern. To test their identity, we analyse the genetic patterns of the A. glabripennis populations sampled across South Korea based on three mitochondrial genes, Cytochrome Oxidase I – leucin tRNA – Cytochrome Oxidase II (COI-tRNALeu- COII). Additionally, we used available sequences of COI - tRNALeu - COII of 104 Chinese and 15 Korean individuals from NCBI. Here we show that Korean population is distinctly divided into two major clades, namely native populations and non-native ones, with 0.7% ~ 1.2% of genetic divergence. Hence, the non-native ones can be divided into two subgroups, which we call Incheon-Seoul complex and Busan-Ulsan complex. We observed that these newly found groups, Incheon-Seoul complex and Busan-Ulsan complex share perfectly identical COI haplotype within each group. This study strongly suggests that newly found populations are introduced respectively and these populations might be derived from very small number of introduced founders.
Lee, Siyoung,Kim, Eunsom,Jhun, Hyunjhung,Hong, Jaewoo,Kwak, Areum,Jo, Seunghyun,Bae, Suyoung,Lee, Jongho,Kim, Busun,Lee, Jungmin,Youn, Sulah,Kim, Somi,Kim, Miyeon,Kim, Hyunwoo,Lee, Youngmin,Choi, Dong American Society for Biochemistry and Molecular Bi 2016 The Journal of biological chemistry Vol.291 No.28
<P>Although it has been established that diabetes increases susceptibility to infections, the role of insulin (INS) in the immune response is unknown. Here, we investigated the immunological function of INS. Proinsulin dimer (pINSd) was a potent immune stimulus that induced inflammatory cytokines, but mature INS was unable to induce an immune response. An affinity-purified rabbit polyclonal antibody raised against mature IL-1α recognized IL-1α and pINS but failed to detect mature INS and IL-1β. Analysis of the pINS sequence revealed the existence of an INS/IL-1α motif in the C-peptide of pINS. Surprisingly, the INS/IL-1α motif was recognized by monoclonal antibody raised against IL-1α. Deleting the INS/IL-1α motif in pINSd and IL-1α changed their activities. To investigate the pINSd receptor, the reconstitution of IL-1 receptor 1 (IL-1R1) in Wish cells restored pINSd activity that was reversed by an IL-1R antagonist. These data suggested that pINSd needs IL-1R1 for inflammatory cytokine induction. Mouse embryo fibroblast cells of IL-1R1-deficient mice further confirmed that pINSd promotes immune responses through IL-1R1.</P>
Seunghyun Lee,Seunghwan Lee 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
The Lamiine subgenus Pseudale Schwarzer, 1925 from Korean peninsula is reviewed. Along with the only recorded species Pterolophia (Pseudale) jiriensis Danilevsky, 1996, we found two additional new species based on morphological and genital difference. Descriptions, photographs of habitus and male genitalia of each species and a key to species of Korea are provided herein. Hence, genital variation of 4 different geographical populations of Pterolophia (Pseudale) jiriensis is discussed.
Stable isotope on the evaluation of water quality in the presence of WWTPs in rivers
Lee, Jaewoong,Park, Taejin,Kim, Min-seob,Kim, Jongmin,Lee, Seunghyun,Lee, Su Kyuong,Lee, Young Sun,Lee, Won-seok,Yu, Soonju,Rhew, Doughee Springer-Verlag 2016 Environmental science and pollution research inter Vol.23 No.18
<P>We investigated the distribution of nitrogen compounds in Han River as well as two tributaries of Tancheon and Jungrangcheon. Particularly, we observed the significant releases of NH4+-N from effluent of wastewater treatment plants (WWTPs) in two tributaries that has resulted in the increases of ammonium nitrogen (NH4+-N) in Han River as well as in Tancheon and Jungrangcheon. Due to the increases of NH4+-N in two tributaries, the larger distribution of delta N-15-NH4+ was observed than those of delta N-15-NO3- in downstream. We calculated the contribution rate of ammonium nitrogen and nitrate nitrogen from effluent to downstream according to the results of stable isotope. The contribution rates of delta N-15-NH4+ from effluent to downstream were significant that ranged between 53 and 100 % in Tancheon and between 27 and 100 % in Jungrangcheon. While the contribution of delta N-15-NO3- was not significant in Tancheon, it was occasionally observed in Jungrangcheon. These results demonstrated that WWTPs are the major sources of NH4+-N in two tributaries, which caused the distinguishable stable isotope of delta N-15-NH4+. Therefore, the stable isotope of delta N-15-NH4+ could be a useful parameter or tracer for the evaluation of NH4-N released from WWTPs in rivers.</P>
Lee, Seunghyun,Lee, Youn-Sun,Choi, Kyeong-Mi,Yoo, Kwang-Sik,Sin, Dong-Mi,Kim, Wonkyun,Lee, Yong-Moon,Hong, Jin-Tae,Yun, Yeo-Pyo,Yoo, Hwan-Soo Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-
<P>Sphingomyelin is the most abundant sphingolipid in mammalian cells and is mostly present in the plasma membrane. A new analytical method using high-performance liquid chromatography (HPLC) was developed to quantify sphingomyelin in mouse plasma and tissues, 3T3-L1 cells, rat aortic smooth muscle cells, and HT-29 cells. Sphingomyelin and dihydrosphingomyelin, an internal standard, were separated by high-performance thin-layer chromatography and simultaneously hydrolyzed with sphingolipid ceramide <I>N</I>-deacylase and sphingomyelinase to release sphingosine and dihydrosphingosine, respectively. Sphingomyelin content was measured by HPLC following <I>o</I>-phthalaldehyde derivatization. Sphingomyelin concentrations in 3T3-L1 cells, rat aortic smooth muscle cells, and HT-29 cells were 60.10 ± 0.24, 62.69 ± 0.08, and 58.38 ± 0.37 pmol/<I><I>μ</I></I>g protein, respectively, whereas those in brain, kidney, and liver of ICR mice were 55.60 ± 0.43, 43.75 ± 0.21, and 22.26 ± 0.14 pmol/<I><I>μ</I></I>g protein. The sphingomyelin concentration in mouse plasma was 407.40 ± 0.31 <I><I>μ</I></I>M. The limits of detection and quantification for sphingomyelin were 5 and 20 pmol, respectively, in the HPLC analysis with fluorescence detection. This sensitivity was sufficient for analyzing sphingomyelin in biological samples. In conclusion, this analytical method is a sensitive and specific technique for quantifying sphingomyelin and was successfully applied to diverse biological samples with excellent reproducibility.</P>
Lee, Seunghyun,Lee, Changkyu,Kim, Bomi,Thao, Le Quang,Lee, Eun Seong,Kim, Jong Oh,Oh, Kyung Taek,Choi, Han-Gon,Youn, Yu Seok Elsevier 2016 Colloids and surfaces Biointerfaces Vol.147 No.-
<P><B>Abstract</B></P> <P>Albumin has been viewed as one of the most attractive biomacromolecules for making nanoparticulate systems due to its biocompatibility and chemical functionality. Thus far, albumin nanoparticles (NPs) are prepared by several limited methods, such as, desolvation, emulsification or high-pressure homogenization. In this article, we introduce a new albumin NPs prototype fabricated <I>via</I> a ‘host’ (β-cyclodextrin)-‘guest’ (adamantane) supramolecular association. These NPs (GC-CD/HSA-ADA NPs) consisted of β-cyclodextrin-modified glycol chitosan (GC-CD) and adamantane-conjugated human serum albumin (HSA-ADA) (GC-CD/HSA-ADA NPs) that were facilely prepared by a consequent dropwise mixing and sonication method. Doxorubicin-loaded GC-CD/HSA-ADA NPs exhibited an appropriate particle size (∼260nm), good physicochemical stability (∼48h), significant HCT116 cell cytotoxicity (IC<SUB>50</SUB>: 0.32μg/ml) and cell internalization. Furthermore, GC-CD/HSA-ADA NPs showed excellent tumor targetability probably due to gp60-mediated transcytosis mechanism because it was markedly accumulated in the tumor site of a HCT116 cell-xenograft mouse. Based on these results, these albumin NPs will be promising for a new NP platform that can be applied for cancer therapy or imaging.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We developed a new prototype of self-assembled albumin nanoparticles. </LI> <LI> The nanoparticles consisted of cyclodextrin-glycol chitosan and adamantane-albumin. </LI> <LI> The nanoparticles were formed <I>via</I> supramolecular cyclodextrin-adamantane association. </LI> <LI> The nanoparticles exhibited remarkable targetability in the HCT116 xenograft tumors. </LI> <LI> These new albumin nanoparticles would be potential as a theranostic carrier. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Seunghyun Lee,Seunghwan Lee 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04
Longhorn beetles (Coleoptera: Cucujiformia: Chrysomeloidea: Cerambycidae) are the most well known coleopteran family by their species-diversity, ecological importance and morphological attraction. Because of their popularity, the immature stages of longhorn beetle are relatively well-studied. However, hardly any study on immature stage of longhorn beetle has been done in Korea until now. Here we provide first intensive research on immature stage of Korean longhorn beetle. Especially, two basic methods used in immature stage research; classical larva-adult matching by rearing and molecular identification by using DNA barcoding will be presented. This study will provide a robust foundation for further study of immature stage identification, DNA barcoding and phylogeny of cerambycidae.
Lee, Changkyu,Seo, Jisoo,Hwang, Ha Shin,Thao, Le Quang,Lee, Seunghyun,Lee, Eun Seong,Lee, Eun Hee,Choi, Han-Gon,Youn, Yu Seok Elsevier 2016 BIOMEDICINE AND PHARMACOTHERAPY Vol.78 No.-
<P><B>Abstract</B></P> <P>Pulmonary fibrosis is a chronic lung disease characterized by inflammation and collagen deposition, with an estimated mortality rate exceeding 70%. Here, we evaluated the therapeutic effectiveness of inhaled tacrolimus-loaded chitosan-coated poly(lactic-<I>co</I>-glycolic acid) nanoparticles (chitosan TAC PLGA-NPs) in a bleomycin-induced pulmonary fibrosis mouse model. Chitosan TAC PLGA-NPs were fabricated using an o/w emulsification diffusion method, and uncoated TAC PLGA-NPs and chitosan TAC PLGA-NPs were spherical with approximate diameters of 320 and 441nm, respectively. The zeta potential of chitosan TAC PLGA-NPs (+13.6mV) was increased significantly by chitosan-coating versus uncoated TAC PLGA-NPs (−28.3mV). The incorporation efficiency of tacrolimus was 37.7%, and the tacrolimus was gradually released until about 5day. Direct inhalation of chitosan TAC PLGA-NPs (TAC 180μg/mouse) twice a week produced marked anti-fibrotic efficacy in mice with bleomycin-induced pulmonary fibrosis, which was much better than the efficacy resulting from daily oral administration (TAC 300μg/mouse) on the basis of hematoxylin/eosin and Masson’s trichrome staining assessments. Imaging of lung deposition showed that chitosan TAC PLGA-NPs were located well in the lungs and gradually faded over 96h. The pulmonary delivery of tacrolimus could be therapeutically efficacious for treating pulmonary fibrosis. TAC-loaded PLGA nanoparticles should be considered to be an efficient sustained-release type inhalation system that reduces administration frequency and relevant side effects.</P>