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( Sung Chul Choi ),( Beom Jin Kim ),( Poong Lyul Rhee ),( Dong Kyung Chang ),( Hee Jung Son ),( Jae J. Kim ),( Jong Chul Rhee ),( Soon Im Kim ),( Young Sil Han ),( Ki Hyeon Sim ),( Seok Nam Park ) 대한소화기기능성질환·운동학회 2011 Gut and Liver Vol.5 No.1
Background/Aims: Although controversial, probiotics and dietary fiber are commonly used for patients with irritable bowel syndrome (IBS). We evaluated the effects of multistrain probiotics on the symptoms of IBS to determine whether the addition of dietary fi ber had an additive effect on constipation-predominant IBS. Methods: A total of 142 participants who met the Rome III criteria were recruited and randomized into a control group or a test group. Participants in the control group received multistrain probiotic fermented milk with Streptococcus thermophilus, Lactobacillus acidophilus and Bifi dobacterium infantis; the participants in the test group received the same probiotic fermented milk mixed with dietary fi ber such as sea tangle extracts, radish extracts and glasswort extracts. The patients were treated for four weeks. Results: Most of the symptoms of IBS, with the exception of fl atulence, stool consistency, and frequency of defecation, signifi cantly improved in both groups. In the analysis of IBS subtypes, especially constipation-predominant IBS, the frequency and duration of defecation and straining at stool were improved more in the test group than in the control group. Conclusions: Dietary fiber had additive benefits for the symptoms of constipation, especially in constipationpredominant IBS. (Gut Liver 2011;5:22-28)
Rapid identification and preparative isolation of antioxidant components in licorice
Sil Lee, Yeon,Ha Kim, Seon,Kyu Kim, Jin,Shin, Hyun-Kyung,Kang, Young-Hee,Yoon Park, Jung Han,Lim, Soon Sung WILEY-VCH Verlag 2010 Journal of Separation Science Vol.33 No.4
<P>This study employed the online HPLC-2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate radical cation (ABTS<SUP>+</SUP><SUP>·</SUP>) bioassay to rapidly determine antioxidant compounds occurring in the licorice extract of Glycyrrhiza uralensis. The negative peaks of the ABTS<SUP>+·</SUP> radical scavenging detection system, which indicated the presence of antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. The ABTS<SUP>+</SUP>-based antioxidant activity profile showed that three peaks exhibited antioxidant activity, and then the high-speed counter-current chromatography technique of preparative scale was successfully applied to separate the three peaks I-III in one step from the licorice extract. The high-speed counter-current chromatography was performed using a two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (6.5:5.5:6:4, v/v). Yields of the three peaks, dehydroglyasperin C (I, 95.1% purity), dehydroglyasperin D (II, 96.2% purity), and isoangustone A (III, 99.5% purity), obtained were 10.33, 10.43, and 6.7% respectively. Chemical structures of the purified dehydroglyasperin C (I), dehydroglyasperin D (II), and isoangustone A (III) were identified by ESI-MS and <SUP>1</SUP>H- and <SUP>13</SUP>C-NMR analysis.</P>
Antioxidant Effects of Spinach (Spinacia oleracea L.) Supplementation in Hyperlipidemic Rats
Sang-Heui Ko,Jae-Hee Park,So-Yun Kim,Seon Woo Lee,Soon-Sil Chun,Eunju Park 한국식품영양과학회 2014 Preventive Nutrition and Food Science Vol.19 No.1
Increased consumption of fresh vegetables that are high in polyphenols has been associated with a reduced risk of oxidative stress-induced disease. The present study aimed to evaluate the antioxidant effects of spinach in vitro and in vivo in hyperlipidemic rats. For measurement of in vitro antioxidant activity, spinach was subjected to hot water extraction (WE) or ethanol extraction (EE) and examined for total polyphenol content (TPC), oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA), and antigenotoxic activity. The in vivo antioxidant activity of spinach was assessed using blood and liver lipid profiles and antioxidant status in rats fed a high fat-cholesterol diet (HFCD) for 6 weeks. The TPC of WE and EE were shown as 1.5±0.0 and 0.5±0.0 ㎎ GAE/g, respectively. Increasing the concentration of the extracts resulted in increased ORAC value, CAA, and antigenotoxic activity for all extracts tested. HFCD-fed rats displayed hyperlipidemia and increased oxidative stress, as indicated by a significant rise in blood and liver lipid profiles, an increase in plasma conjugated diene concentration, an increase in liver thiobarbituric acid reactive substances (TBARS) level, and a significant decrease in manganese superoxide dismutase (Mn-SOD) activity compared with rats fed normal diet. However, administration of 5% spinach showed a beneficial effect in HFCD rats, as indicated by decreased liver TBARS level and DNA damage in leukocyte and increased plasma conjugated dienes and Mn-SOD activity. Thus, the antioxidant activity of spinach may be an effective way to ameliorate high fat and cholesterol diet-induced oxidative stress.
In vivo tracking of intravenously injected mesenchymal stem cells in an Alzheimer’s animal model
Park, Bok-Nam,Lim, Tae Sung,Yoon, Joon-Kee,An, Young-Sil Cognizant Communication Corp. 2018 CELL TRANSPLANTATION Vol. No.
<P><B>Purpose:</B></P><P>The purpose of this study was to investigate how intravenously injected bone marrow-derived mesenchymal stem cells (BMSCs) are distributed in the body of an Alzheimer’s disease (AD) animal model.</P><P><B>Methods:</B></P><P>Stem cells were collected from bone marrow of mice and labeled with Indium-111 (<SUP>111</SUP>In). The <SUP>111</SUP>In-labeled BMSCs were infused intravenously into 3×Tg-AD mice in the AD group and non-transgenic mice (B6129SF2/J) as controls. Biodistribution was evaluated with a gamma counter and gamma camera 24 and 48 h after injecting the stem cells.</P><P><B>Results:</B></P><P>A gamma count of the brain showed a higher distribution of labeled cells in the AD model than in the control group at 24 (p = .0004) and 48 h (p = .0016) after injection of the BMSCs. Similar results were observed by gamma camera imaging (i.e., brain uptake in the AD model was significantly higher than that in the control group). Among the other organs, uptake by the spleen was the highest in both groups. More BMSCs were found in the lungs of the control group than in those of the AD group.</P><P><B>Conclusions:</B></P><P>These results suggest that more intravenously infused BMSCs reached the brain in the AD model than in the control group, but the numbers of stem cells reaching the brain was very small.</P>
Park, Jin-Sil,Lee, Jennifer,Lim, Mi-Ae,Kim, Eun-Kyung,Kim, Sung-Min,Ryu, Jun-Geol,Lee, Jae Ho,Kwok, Seung-Ki,Park, Kyung-Su,Kim, Ho-Youn,Park, Sung-Hwan,Cho, Mi-La American Association of Immunologists 2014 Journal of Immunology Vol. No.
<P>IL-6–mediated STAT3 signaling is essential for Th17 differentiation and plays a central role in the pathogenesis of rheumatoid arthritis. To investigate the molecular mechanism underlying the antirheumatic effects and T cell regulatory effects of STAT3 inhibition, we studied the effects of the JAK 2 inhibitor AG490 on Th17 cell/regulatory T cell (Treg) balance and osteoclastogenesis. AG490 was administered to mice with collagen-induced arthritis (CIA) via i.p. injection, and its in vivo effects were determined. Differential expression of proinflammatory cytokines, including IL-17A, IL-1β, and IL-6, was analyzed by immunohistochemistry. Levels of phosphorylated STAT3 and STAT5 and differentiation of Th17 cells and Tregs after AG490 treatment in our CIA model were analyzed by immunostaining. In vitro development of Th17 cells and Tregs was analyzed by flow cytometry and real-time PCR. AG490 ameliorated the arthritic phenotype in CIA and increased the proportion of Foxp3<SUP>+</SUP> Tregs. In contrast, the proportion of IL-17A–producing T cells and levels of inflammatory markers were reduced in AG490-treated mice. Numbers of p-STAT3<SUP>+</SUP> CD4<SUP>+</SUP> T cells and p-STAT5<SUP>+</SUP> CD4<SUP>+</SUP> T cells were reduced and elevated, respectively, after treatment with AG490. Furthermore, AG490 markedly increased the expression of molecules associated with Treg development (ICOS, programmed cell death protein 1, ICAM-1, and CD103). The development and function of osteoclasts were suppressed by AG490 treatment. Our results suggest that AG490, specifically regulating the JAK2/STAT3 pathway, may be a promising treatment for rheumatoid arthritis.</P>
Development of Plastid InDel Markers to Discriminate Lemons from Other Citrus Groups
Sang Suk Kim,Ho Bang Kim,Kyung Jin Park,Jae Wook Hyun,Cheol Woo Choi,Jae-Ho Joa,Seong Beom Jin,Eun-Sil Kim,Seung Gab Han 한국원예학회 2021 원예과학기술지 Vol.39 No.5
Lemon (Citrus limon), an interspecific hybrid between sour orange and citron, has been widely used as a rootstock along with trifoliate orange. Though lemons are superior to trifoliate orange in terms of their high seed germination rate throughout the year, one of the obstacles to using lemons as rootstocks is the lack of reliable, lemon-specific molecular markers to discriminate buds of the micro-grafted scion from those of the lemon rootstock. In order to obtain lemon-specific molecular markers, we compared the whole-plastid genomes available from four citrus species (lemon, pummelo, sweet orange, and mandarin) and developed seven plastid insertion/deletion (InDel) markers. The plastid InDel markers were applied to 46 citrus accessions that included lemons (17 accessions), grapefruit, mandarin, pummelo, sour orange, orange, papeda, tangor, and tangelo groups. The resulting dendrogram revealed that the citrus accessions used in this analysis could be distinctly classified into seven clusters. Lemons formed a separate cluster and had identical allele sizes for each InDel locus among all accessions investigated. This set of InDel markers could be a useful molecular tool for the rapid and clear discrimination of micro-grafted scions and lemon rootstocks during the production of virus-free citrus trees. The plastid InDel markers with maternal inheritance features can also be used to analyze the phylogenetic origin of various citrus cultivars including lemons.
Sung, Nayoung,Han, Ae Ra,Park, Chan Woo,Park, Dong Wook,Park, Joon Cheol,Kim, Na Young,Lim, Kyung Sil,Shin, Ji Eun,Joo, Chang Woo,Lee, Seung Eun,Kim, Jae Won,Lee, Sung Ki,IVIG Task Force Korean Societ The Korean Society for Reproductive Medicine 2017 Clinical and Experimental Reproductive Medicine Vol.44 No.1
The task force of the Korean Society for Reproductive Immunology recommends intravenous immunoglobulin G treatment in women with reproductive failure, including recurrent pregnancy loss and/or repeated implantation failure, who show cellular immune factors such as abnormal natural killer cell levels, natural killer cell cytotoxicity, and/or type 1 T helper immunity.
Sung, Dong Kyung,Sung, Se In,Ahn, So Yoon,Chang, Yun Sil,Park, Won Soon MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.12
<P>We investigated the role of protease-activated receptor (PAR)-mediated signaling pathways in the biogenesis of human umbilical cord blood-derived mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) and the enrichment of their cargo content after thrombin preconditioning. Immunoblot analyses showed that MSCs expressed two PAR subtypes: PAR-1 and PAR-3. Thrombin preconditioning significantly accelerated MSC-derived EV biogenesis more than five-fold and enriched their cargo contents by more than two-fold via activation of Rab5, early endosomal antigen (EEA)-1, and the extracellular signal regulated kinase (ERK)1/2 and AKT signaling pathways. Blockage of PAR-1 with the PAR-1-specific antagonist, SCH79797, significantly suppressed the activation of Rab5, EEA-1, and the ERK1/2 and AKT pathways and subsequently increased EV production and enriched EV cargo contents. Combined blockage of PAR-1 and PAR-3 further and significantly inhibited the activation of Rab5, EEA-1, and the ERK1/2 and AKT pathways, accelerated EV production, and enriched EV cargo contents. In summary, thrombin preconditioning boosted the biogenesis of MSC-derived EVs and enriched their cargo contents largely via PAR-1-mediated pathways and partly via PAR-1-independent, PAR-3-mediated activation of Rab5, EEA-1, and the ERK1/2 and AKT signaling pathways.</P>