http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Antioxidant Effects of Spinach (Spinacia oleracea L.) Supplementation in Hyperlipidemic Rats
Ko, Sang-Heui,Park, Jae-Hee,Kim, So-Yun,Lee, Seon Woo,Chun, Soon-Sil,Park, Eunju The Korean Society of Food Science and Nutrition 2014 Preventive Nutrition and Food Science Vol.19 No.1
Increased consumption of fresh vegetables that are high in polyphenols has been associated with a reduced risk of oxidative stress-induced disease. The present study aimed to evaluate the antioxidant effects of spinach in vitro and in vivo in hyperlipidemic rats. For measurement of in vitro antioxidant activity, spinach was subjected to hot water extraction (WE) or ethanol extraction (EE) and examined for total polyphenol content (TPC), oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA), and antigenotoxic activity. The in vivo antioxidant activity of spinach was assessed using blood and liver lipid profiles and antioxidant status in rats fed a high fat-cholesterol diet (HFCD) for 6 weeks. The TPC of WE and EE were shown as $1.5{\pm}0.0$ and $0.5{\pm}0.0mg$ GAE/g, respectively. Increasing the concentration of the extracts resulted in increased ORAC value, CAA, and antigenotoxic activity for all extracts tested. HFCD-fed rats displayed hyperlipidemia and increased oxidative stress, as indicated by a significant rise in blood and liver lipid profiles, an increase in plasma conjugated diene concentration, an increase in liver thiobarbituric acid reactive substances (TBARS) level, and a significant decrease in manganese superoxide dismutase (Mn-SOD) activity compared with rats fed normal diet. However, administration of 5% spinach showed a beneficial effect in HFCD rats, as indicated by decreased liver TBARS level and DNA damage in leukocyte and increased plasma conjugated dienes and Mn-SOD activity. Thus, the antioxidant activity of spinach may be an effective way to ameliorate high fat and cholesterol diet-induced oxidative stress.
Ko, Jeong Huy,Park, Rae-su,Jeon, Jong-Ki,Kim, Do Heui,Jung, Sang-Chul,Kim, Sang Chai,Park, Young-Kwon Elsevier 2015 Journal of industrial and engineering chemistry Vol.32 No.-
<P><B>Abstract</B></P> <P>Deactivated waste activated carbon due to the deposition of boron in the exhaust gas from an incinerator was regenerated by the treatment with surfactant, HCl, and NH<SUB>3</SUB>. The catalysts were characterized using BET surface area, ICP, XPS and FT-IR spectra. Sequential treatment with surfactant and HCl removed the adsorbed boron very effectively. NH<SUB>3</SUB> treatment increased the number of functional nitrogen groups in regenerated sample. For NH<SUB>3</SUB> selective catalytic reduction (SCR) of NO, sequential treatments with surfactant, HCl, and NH<SUB>3</SUB> showed the highest DeNOx activity, attributed to the increased metal removal efficiency, specific surface area and amount of nitrogen functional groups on the surface.</P>
Ko, Seok-Chun,Lee, Seung-Hong,Kang, Sung-Myung,Ahn, Ginnae,Cha, Seon-Heui,Jeon, You-Jin The Korean Society for Marine Biotechnology 2011 한국해양바이오학회지 Vol.5 No.4
As a rapid and quick bioactive compound evaluation technique, we utilized an automatic system of high throughput screening (HTS) to investigate ${\alpha}$-glucosidase inhibitory efficacy of seaweeds, collected from Jeju Island in Korea. In this study, different extracts with methanol at $20^{\circ}C$ and $70^{\circ}C$ from 23 species of brown seaweeds and 22 species of red seaweeds and 9 species of green seaweeds were subjected to HTS. Of the brown seaweeds tested, Myelophycus simplex (20B3), Ishige sinicola (20B5, 70B5), Colpomenia sinuosa, (20B14, 70B14), Hizikia fusiforme (20B21), Ishige okamurai (70B22) and Ecklonia cava (70B23) showed significantly high ${\alpha}$-glucosidase inhibitory activity with 96.52%, 98.34%, 98.37%, 80.49%, 96.16%, 76.32%, 98.32% and 98.12%. Schizymenia dubyi (20R15), Gelidium amansii (20R16) and Polysiphonia japonica (70R22) amomng the red seaweeds showed remarkable ${\alpha}$-glucosidase inhibitory activity more than 95%. On the other hand, the green seaweeds showed poor ${\alpha}$-glucosidase inhibitory activities (less the10%) at 1 mg/ml.
Ko, Ah-Young,Abd El-Aty, A.M.,Jang, Jin,Choi, Jeong-Heui,Rahman, Md. Musfiqur,Kim, Sung-Woo,Shin, Ho-Chul,Shim, Jae-Han The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.2
In the present study, brown rice was steeped in 20 % wettable powder fludioxonil for 24 h, subsequently germinated, and transplanted in paddy fields. The harvested rice was tested at 156 days to detect residue levels using gas chromatography-nitrogen phosphorus detector. Validation was carried out to assess the following parameters: linearity, limit of detection and limit of quantitation (LOQ), recovery, and storage stability. Using matrix-matched calibrations, the determination coefficients were >0.999 in both matrices. Mean recoveries were 73.5-101.0 % with relative standard deviations <10 % in both matrices. The LOQ (0.006 mg/kg) was lower than the maximum residue limit (MRL = 0.02 mg/kg) set by the Ministry of Food and Drug Safety, Republic of Korea. The developed method was applied successfully, and no residues were detected in field-incurred rice and/or rice straw samples.
Ko, Hyun Myung,Lee, Sung Hoon,Bang, Minji,Kim, Ki Chan,Jeon, Se Jin,Park, Yeong-Min,Han, Seol-Heui,Kim, Hahn Young,Lee, Jongmin,Shin, Chan Young Elsevier 2018 Biochemical and biophysical research communication Vol.495 No.1
<P><B>Abstract</B></P> <P>The high concentrations of nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) in activated glial cells in response to neuroinflammatory stimuli have neurotoxic effects on the brain. At basal levels, iNOS expression is low, and proinflammatory stimuli induce iNOS expression in astrocytes, microglia, and oligodendrocytes. Fyn, a non-receptor tyrosine kinase, regulates iNOS expression in several types of immune cells. However, its role in stimulated astrocytes is less clear. In this study, we investigated the role of Fyn in the regulation of lipopolysaccharide (LPS)-induced iNOS expression in astrocytes from mice and rats. Intracerebroventricular LPS injections in cortical regions enhanced iNOS mRNA and protein levels, which were increased in Fyn-deficient mice. Accordingly, LPS-induced nitrite production was enhanced in primary astrocytes cultured from Fyn-deficient mice or rats. Similar results were observed in cultured astrocytes after the siRNA-induced knockdown of Fyn expression. Finally, we observed increased LPS-induced extracellular signal-regulated protein kinase (ERK) activation in Fyn-deficient astrocytes. These results suggested that Fyn has a regulatory role in iNOS expression in astrocytes during neuroinflammatory responses.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fyn regulates LPS-induced iNOS expression in the cortices. </LI> <LI> Fyn modulates iNOS expression and nitrite levels in LPS-stimulated astrocytes. </LI> <LI> Fyn regulates iNOS expression in LPS-stimulated astrocytes via ERK phosphorylation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
( Sang-cheol Kim ),( Jung-il Kang ),( Jin-won Hyun ),( Ji-hoon Kang ),( Young-sang Koh ),( Young-heui Kim ),( Ki-ho Kim ),( Ji-hee Ko ),( Eun-sook Yoo ),( Hee-kyoung Kang ) 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.4
4-O-methylhonokiol, a neolignan compound from Magnolia Officinalis, has been reported to have various biological activities including hair growth promoting effect. However, although transforming growth factor-β (TGF-β) signal pathway has an essential role in the regression induction of hair growth, the effect of 4-O-methylhonokiol on the TGF-β signal pathway has not yet been elucidated. We thus examined the effect of 4-O-methylhonokiol on TGF-β-induced canonical and noncanonical pathways in HaCaT human keratinocytes. When HaCaT cells were pretreated with 4-O-methylhonokiol, TGF-β1-induced G1/G0 phase arrest and TGF-β1-induced p21 expression were decreased. Moreover, 4-O-methylhonokiol inhibited nuclear translocation of Smad2/3, Smad4 and Sp1 in TGF-β1-induced canonical pathway. We observed that ERK phosphorylation by TGF-β1 was significantly attenuated by treatment with 4-O-methylhonokiol. 4-O-methylhonokiol inhibited TGF-β1-induced reactive oxygen species (ROS) production and reduced the increase of NADPH oxidase 4 (NOX4) mRNA level in TGF-β1-induced noncanonical pathway. These results indicate that 4-O-methylhonokiol could inhibit TGF-β1-induced cell cycle arrest through inhibition of canonical and noncanonical pathways in human keratinocyte HaCaT cell and that 4-O-methylhonokiol might have protective action on TGF-β1-induced cell cycle arrest.
Antioxidant Effects of Spinach (Spinacia oleracea L.) Supplementation in Hyperlipidemic Rats
Sang-Heui Ko,Jae-Hee Park,So-Yun Kim,Seon Woo Lee,Soon-Sil Chun,Eunju Park 한국식품영양과학회 2014 Preventive Nutrition and Food Science Vol.19 No.1
Increased consumption of fresh vegetables that are high in polyphenols has been associated with a reduced risk of oxidative stress-induced disease. The present study aimed to evaluate the antioxidant effects of spinach in vitro and in vivo in hyperlipidemic rats. For measurement of in vitro antioxidant activity, spinach was subjected to hot water extraction (WE) or ethanol extraction (EE) and examined for total polyphenol content (TPC), oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA), and antigenotoxic activity. The in vivo antioxidant activity of spinach was assessed using blood and liver lipid profiles and antioxidant status in rats fed a high fat-cholesterol diet (HFCD) for 6 weeks. The TPC of WE and EE were shown as 1.5±0.0 and 0.5±0.0 ㎎ GAE/g, respectively. Increasing the concentration of the extracts resulted in increased ORAC value, CAA, and antigenotoxic activity for all extracts tested. HFCD-fed rats displayed hyperlipidemia and increased oxidative stress, as indicated by a significant rise in blood and liver lipid profiles, an increase in plasma conjugated diene concentration, an increase in liver thiobarbituric acid reactive substances (TBARS) level, and a significant decrease in manganese superoxide dismutase (Mn-SOD) activity compared with rats fed normal diet. However, administration of 5% spinach showed a beneficial effect in HFCD rats, as indicated by decreased liver TBARS level and DNA damage in leukocyte and increased plasma conjugated dienes and Mn-SOD activity. Thus, the antioxidant activity of spinach may be an effective way to ameliorate high fat and cholesterol diet-induced oxidative stress.
( Sang Ho Lee ),( Hye Young Koo ),( Jung Hyun Kim ),( You Na Ko ),( Jang Heui Yi ),( Yun Chan Kang ),( Hye Moon Lee ),( Jung Yeul Yun ) 대한금속재료학회 ( 구 대한금속학회 ) 2010 재료마당 Vol.23 No.3
Fine size BaMgAl10O17:Eu phosphor powders were prepared by spray pyrolysis from a spray solution with ethylenediaminetetraacetic acid, citric acid, and NH4F flux. The precursor powder prepared by spray pyrolysis has a hollow, thin-walled structure and was transformed into BaMgAl10O17:Eu phosphor powders with fine size and a plate-like morphology. The phosphor powders had pure hexagonal BAM phases even at a low temperature of 1100°C. The mean sizes of the phosphor powders prepared from a spray solution with 6wt. % NH4F of the phosphor were 1.1 μm, 2.4 μm, and 1.9 μm at post-treatment temperatures of 1100°C, 1150°C, and 1300°C. The maximum photoluminescence intensity of the phosphor powders post-treated at 1150°C was 93% of that of commercial BAM:Eu phosphor powders.