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A Study on the Multiple Trial of Unrelated Question Models
이기성,홍기학,Lee, Gi-Sung,Hong, Ki-Hak 한국데이터정보과학회 2002 한국데이터정보과학회지 Vol.13 No.1
In this paper, we proposed multiple trial unrelated question models that more efficient by reducing the variance of the estimate than single trial unrelated question models investigated by Greenberg et al.'s (1969) and Kim et al.'s (1992) an d Lee & Hong's (1998).
Young-Chul Kim,Hyun-Hee Chae,Sang-Heock Oh,Seung-Ho Choi2,Moon-Pyo Hong3,Gi-Heum Nam,Jae-Yoon Choi,Hyun-Sook Choi,Kyu-Song Lee 한국환경생태학회 2015 한국환경생태학회지 Vol.29 No.2
Yongneup wetland protected area, the only high moor in Korea, is a core area to conserve biodiversity. Even though the Yongneup wetland protected area is relatively small, various plant species are distributed in the Yongneup wetland protected area because it includes various habitats showing different environmental gradients. Vascular plants distributed in the Yongneup wetland protected area were identified as a total of 376 taxa that is composed of 73 families, 217 genera, 322 species, 3 subspecies, 44 varieties and 7 forms. For endangered plants designated by the Ministry of Environment, 5 species including Trientalis europaea var. arctica, Lilium dauricum, Halenia corniculata, Lychnis wilfordii and Menyanthes trifoliata were found and 34 taxa were confirmed to be distributed only in the mountainous wetland habitats. Regarding naturalized plants, a total of 11 taxa were distributed, but most of them were distributed in the areas where artificial interference has occurred. And in areas inside the wetlands that are relatively well preserved, 2 species of Bidens frondosa and Erigeron annuus were observed. In this study, the occurrence and distribution of Pseudostellaria baekdusanensis M. Kim, which was recently found in Mt. Baekdu and reported as a new species, were identified in the Yongneup wetland protected area. A wetland is a very vulnerable area to drastic environmental changes and damages to its ecosystem could cause the extinction of rare plant species which are distributed only in the wetlands. Therefore, it is mandatory that current status of the Yongneup wetland protected area is evaluated and actions to prevent rapid environmental changes are taken. Fourteen separate investigations were conducted in 2013 and another four in 2014, to evaluate current status of the Yongneup wetland protected area. These investigations have provided us the basic information for future actions of conservation and restoration.
Hong, Heeji,Kim, So-Jeong,Min, Ui-Gi,Lee, Yong-Jae,Kim, Song-Gun,Roh, Seong Woon,Kim, Jong-Geol,Na, Jeong-Geol,Rhee, Sung-Keun International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.5
<P>A strictly anaerobic, mesophilic, iron-reducing bacterial strain, IRF19<SUP>T</SUP>, was isolated from coal-contaminated soil in the Republic of Korea. IRF19<SUP>T</SUP> cells were straight, rod-shaped, Gram-staining-negative and motile by means of flagella. The optimum pH and temperature for their growth were determined to be pH 7.5–8.0 and 40 °C, while the optimum range was pH 6.5–10.0 and 20–45 °C, respectively. Strain IRF19<SUP>T</SUP> did not require NaCl for growth but it tolerated up to 2 % (w/v). Growth was observed with yeast extract, <SMALL>d</SMALL>-glucose, <SMALL>d</SMALL>-fructose, <SMALL>d</SMALL>-ribose, <SMALL>d</SMALL>-mannitol, <SMALL>d</SMALL>-mannose, <SMALL>l</SMALL>-serine, <SMALL>l</SMALL>-alanine and <SMALL>l</SMALL>-isoleucine. Fe(III), elemental sulfur, thiosulfate and sulfate were used as electron acceptors. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain IRF19<SUP>T</SUP> is affiliated to the family <I>Clostridiaceae</I> and is most closely related to <I>Salimesophilobacter vulgaris</I> Zn2<SUP>T</SUP> (93.5 % similarity), <I>Geosporobacter subterraneus</I> VNs68<SUP>T</SUP> (93.2 %) and <I>Thermotalea metallivorans</I> B2-1<SUP>T</SUP> (92.3 %). The major cellular fatty acids of strain IRF19<SUP>T</SUP> were C<SUB>14 : 0</SUB>, iso-C<SUB>15 : 0</SUB> and C<SUB>16 : 0</SUB>, and the profile was distinct from those of the closely related species. The major respiratory quinone of strain IRF19<SUP>T</SUP> was menaquinone MK-5 (V-H<SUB>2</SUB>). The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid and two unknown polar lipids. The G+C content of the genomic DNA of strain IRF19<SUP>T</SUP> was determined to be 37.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic results, strain IRF19<SUP>T</SUP> is considered to represent a novel species of a novel genus of the family <I>Clostridiaceae</I> , for which we propose the name <I>Anaerosolibacter carboniphilus</I> gen. nov., sp. nov., with the type strain IRF19<SUP>T</SUP> ( = KCTC 15396<SUP>T</SUP> = JCM 19988<SUP>T</SUP>).</P>
Pioglitazone Improves Oocyte Maturation and Subsequent Developmental Competence of Porcine Embryos
Sang-Gi Jeong,Seung-Eun Lee,Won-Jae Kim,Jae-Wook Yoon,Chan-Oh Park,Hyo-Jin Park,Dong-Yong Hong,Eun-Young Kim,Se-Pill Park 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
The optimization of culture condition is both basic and important for improving oocyte maturation, subsequent development of preimplantation embryo, and further implantation. This study performed to confirm effect of pioglitazone (PIO) treatment directly on porcine oocyte during in vitro maturation, further development of embryo. Immature oocytes were cultured in IVM medium, containing 0, 0.01, 0.1, 1, and 10 μM pioglitazone (0, 0.01, 0.1, 1, and 10 PIO, respectively) for 44 h. Although both oocyte survival and polar body emission did not significantly differ between the control and PIO-treated groups, percentage of normal spindle formation and chromosome alignment was increased in the 1 PIO-treated group than in the control group (control, 60.9±8.2%; and 1 PIO, 81.5±5.9%, p<0.1). Level of GSH was significantly increased in 1 PIO-treated group than in the control group (control, 100.0±5.0%; and 1 PIO, 119.8±5.2%, p<0.05), while the level of ROS was not differ in all groups. The rate of blastocyst was significantly higher in the 1 PIO-treated group than in the control at day 6 (control, 29.6±2.5%; and 1 PIO, 37.9±2.3%, p<0.05) and 7 (control, 35.1±1.6%; and 1 PIO, 41.8±2.7%, p<0.05), respectively. These results indicated that PIO treatment during IVM showing beneficial effects on oocyte and embryonic development. Pioglitazone will contribute to enhancing efficiency of in vitro production.