Effect of Non-animal-Derived Nitrogen Sources on the Production of Hyaluronic Acid by Streptococcus sp. KL0188

Lee, Gil-Yong,Ha, Suk-Jin,Jung, Jong-Hyun,Seo, Dong-Ho,Park, Jong-Yul,Kim, Su-Rin,Park, Nam-Woo,Kweon, Dong-Keon,Park, Sang-Hoo,Park, Cheon-Seok The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.3

Hyaluronic acid (HA) is a linear high-molecular-weight polysaccharide with useful biomedical applications. Streptococcus zooepidemicus, a typical HA-producing bacterium, requires an animal-derived nitrogen source such as tryptone, peptone or sheep blood as a nutrient. Sixteen non-animal-derived (NAD) nitrogen sources were tested as a replacement for the expensive animal-derived nitrogen sources, which may have safety issues. Among the sixteen tested NAD nitrogen sources, a yeast-derived nitrogen source (YE 0251) showed the highest HA productivity, which was equivalent to the control HA production medium containing tryptone in a 5-L jar and in 3,000-L industrial fermentations. In the 3,000-L fermentation, YE 0251 increased cell mass (dry cell weight) and HA production by 11% and 8%, respectively, compared with the control HA production medium. The fmal specific volumetric productivity (0.41 g/L h) was improved by about 70% after reducing the fermentation time from 20 h to 12 h, compared to the conventional production medium.

작은 피복두께를 갖는 GFRP 보강근의 부착특성

하상수;최동욱 한경대학교 2007 論文集 Vol.39 No.-

본 연구는 작은 피복 두께를 갖고 따라서 콘크리트 쪼갬 파괴가 발생하는 GFRP 보강근의 부착 특성을 파악하여, 추후 GFRP 보강근의 정착 길이 설계식을 제안하기 위한 기초 자료로서 제공하는 것이 목적이었다. 묻힘 길이, 피복 두께, 보강근 직경, 보강근 종류를 변수로 하여 총 38개의 실험을 수행하였다. 묻힘 길이는 15, 30, 45로 하고, 순 피복 두께는 0.5, 1.0, 1.5, 2.0로 계획하였다. 그리고 보강근 직경 2종류(D10, D13), 보강근 3종류(국내 2종, 국외 1종)를 사용하였다. 인발 실험을 통하여 변수에 따른 파괴 양상, 하중-변위 곡선, 평균 부착 강도 등의 부착 거동을 실험적으로 파악하였다. 실험 결과, 파괴 양상은 보강근 인장 파괴 또는 보강근 주위의 콘크리트 쪼갬 파괴에 의해 최종 파괴되었으며, 뽑힘 파괴는 발생하지 않았다. 평균 부착 응력은 동일 조건에서 피복 두께가 0.5, 1.0, 1.5, 2.0 순으로 커질수록 높게 나타났으며, 또한 묻힘 길이가 45, 30, 15 순으로 작을수록 높게 나타났다. 그러나 보강근 크기 및 보강근 종류를 변수로 한 실험 결과는 큰 차이가 없었다. 피복 두께가 0.5, 1.0인 실험체는 묻힘 길이에 관계없이 모두 쪼갬 파괴가 발생하였으며 평균 부착 응력은 각각 3.90MPa, 4.90MPa로 나타났다. 또한, 피복 두께가 1.5, 2.0인 경우에는 보강근 인장 파괴와 쪼갬 파괴가 같이 공존하고, 이 가운데 쪼갬 파괴된 실험체의 평균 부착 강도는 각각 5.95MPa와 8.31MPa로 나타나 피복 두께가 0.5, 1.0인 실험체에 비해 평균 부착 강도가 높았으나, 편차가 심한 경향을 보여주었다.

위상모형을 활용한 공간정보의 가시화기법에 관한 연구

하수욱,이성호 釜山大學校 都市問題硏究所 2002 都市硏究報 Vol.13 No.-

This paper presents applicable methods for change of spatial attributes in ARC/INFO environment, general GIS package. To test the validity of the proposed method, I investigate the change of Manufacturing industry in Busan from 1995 to 1999. The results are as follows : First, functions in ARC/INFO environment were utilized to present methodologies to apply these models. Second, compared with existing spatial model using kriging or trend surface model, topological method could efficiently display spatial data in global and local scale. Third, as method to make up topological model, each point value is represented. Through this procedure, it is more effective to understand the change of spatial data.

白地어음의 補充權의 行事에 관한 再考

하삼주,이상수 건국대학교 사회정책연구소 2003 사회과학연구 Vol.16 No.-

부산물 비료에 발효미생물의 첨가가 잎들깨의 생장특성과 수량 증수효과

하상영,박선일,정대수,여수진 東亞大學校 1999 東亞論叢 Vol.36 No.-

공시재료 부산물 비료 3종류에 발효미생물을 혼합하여 재배한 잎들깨의 생장 특성조사 결과는 다음과 같다. 초장에 있어 공기 발효미생물을 혼합한 부산물 비료는 대풍, 바이오청, 풍작 순이며, 엽수의 채엽증가는 바이오청, 풍작, 대풍 순으로 채엽의 수가 증가하였으며, 엽의 길이느 ㄴ처리구에서 엽의 길이가 길어지는 경향은 잇고, 조사 횟수에 따라 엽의 길이에 차이가 있었다. 엽의 폭은 엽의 길이와 같은 경향이 잇으며, 엽의 건물비율은 부산물 비료와 발효미생물의 혼합 유무와 환경요인에 따라 건물비율이 증감하는 것으로 판단되며, 줄기 30cm의 직경은 바이오짐+대풍, 하이그린 J+대풍, 아제론+대풍이 직경이 커지는 경항이 있다. This study was conducted to elucidate the growth characteristics of perilla, Perilla Frutescens, which was cultivated by mixture Fermentative microbe into three types Organic compost. In the height of perilla, Daepung mixed with Fermentative microbe showed the highest level and followed by Biochong and Pungchag in order. Number of the harvested leaves were the highest in Biochong, Pungchag and Daepung in order. Leaf length and leaf width were more effective at the three kinds on Organic compost than Control. The rate of dried leaf weight was decided by Organic compost, Fermentative microbe and Environmental factor. Diameter of perilla stem at 30cm above the ground increased mixture Fermentative microbe and Organic compost and was the thichest in Biojim+Daepung, Higreen-J+Daepung and Ajelon+Daepung.

담도에 국한되어 증상을 유발한 석회쓸개즙 2 예

윤성우,이지영,하경호,이수경,한나,배효근,박선자 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Limy bile is a rare condition characterized by excessive precipitation of calcium carbonate in the gallbladder or bile duct. Recently we experienced two cases with obstructive jaundice in which limy bile in the common bile duct but not in the gallbladder for one case, and in the common bile duct and intra hepatic duct but not in the gallbladder for another case. The patients were treated by endoscopic sphincterotomy. Here, we report the cases and review the literature.

신축이음장치에서 앵커의 인발 성능 및 나사 풀림 방지를 위한 앵커 시스템 개발

하상수;최동욱 한경대학교 2008 論文集 Vol.40 No.-

The failure of expansion joint for bridge generally occurrs in the non-shrinking mortar while other problem is the release of anchors in expansion joint due to the impact and vibration by traffic on the bridge. In this study, to overcome the failure of expansion joint by the failure of mortar, the elastic mortar is developed. The pull-out capacity of developed elastic mortar was compared with that of non-shrinking mortar. Moreover the anchor system which can be changed easily and prevent a fracture of expansion joint is developed 교량 신축이음장치의 파손 유형은 주로 후타재(무수축 모르타르)와 관련된 부분에서 많이 발생된다. 또 다른 문제는 중차량 통과에 따른 충격과 진동하중으로 인해서 신축이음장치를 후타재에 고정하기 위해서 사용하는 앵커 풀림 현상이다. 본 연구에서는 무수축 모르타르의 파괴에 따른 신축이음장치의 파손을 방지하기 위해 탄성콘크리트를 개발하였다. 개발된 탄성콘크리트와 현재 주로 사용하고 있는 무수축 모르타르의 인발성능을 비교하였다. 또한, 후타재와 신축이음장치를 일체화하여 후타재의 파손을 방지하고 신축이음장치를 쉽게 교체할 수 있는 앵커 시스템을 개발하였다

강선 이음형 PC 보-기둥 내부 접합부의 구조적 거동에 관한 실험적 연구

하상수,김승훈,문정호,이리형,임주혁 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

In this study, new moment-resisting precast concrete beam-column joint is proposed for moderate seismic regions. it is attempt to devise connection detail which makes workability improve and confirm effective stress transfer in the region of connection. It has the connection details which help working process simplified and construction cost reduced. as replace 9.3㎜, 7strand for reinforcement. Fore specimens for partial tension test and three specimens for beam-column interior joint test were producted. An experimental study was carried out to investigate the joint behavior subjected to reverse cyclic loading and constant axial compressive load. The test variables was transverse reinforcement in joint. Structural performance of joint is evaluated on the basis of connection strength, stiffness, energy dissipation, and drift capacity. Based on the test results, the precast connection is capable of matching or exceeding the performance of the monolithic connection and thereby provides the sufficient moment-resisting behavior to be applicable for moderate seismic regions.

우리 나라 보건관리대행서비스 평가 연구

하은희,조수헌,김선민,주영수,한상환,하미나,권호장,홍윤철,김창엽 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.1

Group occupational health service programme started in 1990 is one of the measures to cope with limited human and financial resources in occupational health. The programme has expanded rapidly to include 52 institutions, private as well as public, all over the country. In spite of its potential impact on health of employees and practice of occupational health in small and medium sized industries, comprehensive evaluation in terms of quality has not been tried. This study has aims to develop the criteria to assess the quality of newly developed group occupational health service programme, and to investigate the quality of institutions, and finally to develop policies for the quality improvement. 1) Criteria development : By defining occupational health services, in particular for small and medium sized industries, as one of the primary health care, followings are included as core elements of qualitative occupational health programme; accessibility, continuity, comprehensiveness, technical quality, intersectoral collaboration, emphasis on preventive services, community participation, and adequacy. Again each element is divided into five major components of national health system infrastructure developed by the World Health Organization; development of health resources, organized arrangement of resources, delivery of health care, economic support, and management. In turn, each component is categorized into three aspects of quality assessment, structure, process and outcome. Expert panel selected several criteria for each category to evaluate the programme. Criteria were modified according to each group of interviewees, to produce two sets of questionnaire, one for chief operating officer and another for nurses in the institutions, and the chief operating officer and workers in the workplace. 2) Subject : Of all 52 institutions, 25 voluntarily participated in the survey. At individual institution, chief operating officer and practicing nurses were interviewed in depth. After intensive education for interviewees, every interviewees, every interview was performed with standardized guideline and questionnaire. The quality of the 'Group occupational health service programme' was found to be lower than expected. Especially in continuity, comprehensiveness, technical quality, community participation and adequacy, lower quality in structural aspect was commonly identified throughout all the institutions,. Quality in terms of accessibility and continuity highly varied among institutions., To improve quality of the programme, more comprehensive and systematic programme such as accreditation has to be introduced. In addition, human resources, governmental fund and information systems for individual workers are to be developed. As a long range plan, integration of occupational health services into the national health systems and pooling of financial resources and planned allocation should be considered.

良好한 照明이 우리 生活에 미치는 影響

金正洙,李鍾夏,徐相壽 忠州大學校 1968 한국교통대학교 논문집 Vol.2 No.-

In this paper ; I examined the condition for proper illumination with the study of its influence on our daily life and our health, and of the ideal illumination of our houses, buildings and facfories, on the standpoint of human engineering and I made an attempt to study on these problems through the examination in the influence of proper illumination on our sight, with one thousand children of the fourth, the fifth, and the sixth grade of the primary school in the city of Chungju as an object of the study.