상악 제1 및 제2소구치의 발치공간 폐쇄기전에 대한 3차원 유한요소 해석의 비교 연구

고신애,임원희,박선형,전윤식 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.6

교정 치료에서 발치공간 폐쇄는 치열을 이루는 모든 치아의 연속적인 이동으로 이루어지므로 그 기전은 복잡하다. 특히 전치부 치축을 적절히 유지하면서 구치부 고정원을 조절하는 과정은 정교함을 요하기 때문에 입체적 분석을 통한 치아이동 양상에 대한 이해가 필요하다. 지금까지의 유한요소 분석은 초기 응력분포를 관찰하여 치아 이동 양상을 예측해 보는데 그쳤지만 이러한 양상만으로 정확한 치아이동 결과를 추정하는 데에는 한계가 있었다. 따라서 본 연구에서는 3차원 유한요소 모델을 이용하여 상악 제1소구치 및 제2소구치 발치공간 폐쇄 시 전치부와 구치부의 입체적인 이동 양상을 단계별로 비교하여 그 기전을 규명하고자 하였다. 자연치의 크기 및 형태를 갖는 상악 치아들과 브라켓, 교정용 호선 및 치조골부를 3차원 레이저 스캐너로 스캐닝한 후 사면체 요소의 유한요소모델을 제작하였다. 0.017" x 0.025" 스테인레스 강 호선에 제작된 bull 루프 후방에 10˚의 gable bend를 부여하고 한 번에 2mm씩 12회 활성화시켜 발치공간을 폐쇄시켰다. 그 결과 제1소구치를 발치한 경우 제2소구치 발치에 비해 전치부의 후방 이동량이 많았으며 구치부의 전방 이동량은 더 적게 나타났다. 전치부에서는 제1, 2소구치 발치 모두 비슷한 미약한 정출을 동반하였고 치축의 변화량은 제1소구치 발치에서 더 크게 나타났다. 또한 제2소구치 발치 시 고정원의 협측 이동량이 더 크게 나타났다. Objective: The aim of this study was to compare the differences in closing extraction spaces between maxillary first premolar and second premolar extractions using 3-dimensional finite element analysis (FEA). Methods: Maxillary artificial teeth were selected according to Wheeler's dental anatomy. The size and shape of each tooth, bracket and archwire were made from captured real images by a 3D laser scanner and FEA was performed with a 10-noded tetrahedron. A 10˚ gable bend was placed behind the bull loop on a 0.017" x 0.025" archwire. The extraction space was then closed through 12 repeated activating processes for each 2mm of space. Results and Conclusions: The study demonstrated that the retraction of anterior teeth was less for the second premolar extraction than for the first premolar extraction. The anterior teeth showed a controlled tipping movement with slight extrusion, and the posterior teeth showed a mesial-in rotational movement. For the second premolar extraction, buccal movement of posterior teeth was highly increased.

병원의무기록과에서 생성되는 각종 통계와 그전산화율에 대한 조사

서승원,김광환,김석재,박석건,서진숙,신종연,이경미,최명애 대한의료정보학회 1999 Healthcare Informatics Research Vol.6 No.3

정확한 국가보건통계 데이터베이스는 보건정책의 기초가 되지만, 우리나라에서는 아직 신뢰할만한 통계를 생산해내지 못하고 있는 것이 사실이다. 의료보험자료를 기초로 해서 만드는 보건통계가 있지만 여러 면에서 신뢰도가 떨어진다는 의견들이 많다. 이에 저자들은 대안으로서 각 병원의 의무기록실에서 생성하고 있는 통계들이 국가보건통계의 기초가 될 수 있는 지를 알아보기 위해 78개 병원을 대상으로 통계 생성여부와 전산화 정도를 조사하였다. 설문은 병원표준화 심사와 서비스평가, OECD에서 요구하는 통계들을 참고로 하여 작성하였으며, 해당 통계를 생성하고 있는지, 통계생성 작업이 어느정도 전산화되어 있는지를 물었다. 응답한 병원의 50% 이상이 통계를 생성하고 있다고 하였으며, 통계를 생성하고 있는 병원에서 전산망을 이용하지 않고 수작업으로 하고있는 비율은 1.3%에서 7.7% 사이였다. 설문서에서 질문한 통계를 생성하지 않고 있다고 응답한 병원의 대부분은 전산화 되어있지 않았다. 이상의 결과로부터 각 병원의 의무기록실에서는 많은 부분 국가보건 통계에 필요한 각종 통계량들을 생성해 내고 있으며, 통계 생성 방법은 전산화 되어있고, 따라서 이를 수집, 가공하면 국가보건통계 데이터베이스를 구축할 수도 있을 것이라는 결론을 얻었다. 이를 위해서는 통계량을 정의하는 표준화 사업이 선행되어야 할 것이다 We surveyed the generation rate of health statistics by medical records offices of the 78 hospitals and its automation rate using computerized hospital information system. Structured questionnaire was given to one medical record officer of each hospital. Items in the questionnaire was selected from statistics required for hospital service evaluation or OECD health statistics. More than 50% of the medical record office generated questioned health statistics, and most of them was automated. Because many of the medical record offices of the hospitals are producing essential health statistics and automated, the is a possibility that we can collect and use these datas to build up national health database if adequate standardization procedure can be implemented.

계획되지 않은 아동의 입원시 정보제공이 어머니 스트레스 대처에 미치는 효과

최명애,박승현,권원경,김선구,안혜영,김윤경 서울대학교 간호대학 간호과학연구소 1999 간호학 논문집 Vol.13 No.1

The purpose of this study was to explore the effect of informational intervention on coping of mother's stress in unplanned childhood hospitalization. Thirty four mothers of the hospitalized children, 24 months to 60 months old, were randomly assigned to experimental and control group. Child behavioral information and parental role information was given to experimental group by booklet and cassette tape. The state-trait anxiety was measured within 12 to 24 hours following hospitalization, the state anxiety was measured during from 24 hours to 48 hours, and the parent participation with care of children, and the parent support during invasive procedures was measured during 48 hours to 72 hours after hospitalization. The data was collected from May, 1997 to October, 1998 at B hospital in Seoul. Data was analyzed with X²-test, non paired t-test, ANCOVA test and Wilcoxon test using SAS program. Results were obtained as follows: 1. There were no difference in the state anxiety during the period of hospitalization and after discharging from hospital between experimental and control group. 2. Experimental group showed significantly higher scores on the parent participation with care of children than control group(P=0.03). 3. The parent support during invasive procedures of experimental group was significantly greater than that of control group(P=0.0167). The results suggest that information intervention regarding child behavioral information and parental role information may enhance both the parent participation with care of children and the parent support during invasive procedures.

복막투석복막염 환자에서 발생한 창자벽공기증 1예

정선영,나지훈,최윤정,고성애,조규향,박종원,도준영,윤경우 영남대학교 의과대학 2009 Yeungnam University Journal of Medicine Vol.26 No.1

Peritonitis is a serious problem in patients undergoing peritoneal dialysis. Rarely pneumatosis intestinalis can occur as a complication of this infectious process. Pneumatosis intestinalis is a potential life threatening condition with a challenging management. The mortality of peritoneal dialysis patients with pneumatosis intestinalis secondary to mesenteric ischemia is almost 100%. We describe a rare case of pneumatosis intestinalis in a peritoneal dialysis patient who developed Staphylococcus aureus peritonitis which was initially treated with appropriate antibiotics. Since initial response to therapy was not achieved, an abdominal computerized tomography was done which revealed a pneumatosis intestinalis. A laparotomy was performed and small bowel necrosis was seen. A segmented resection with ileostomy, jejunostomy was done. Though surgical treatment was performed, the patient died in 2 weeks after admission. Pneumocystitis intestinalis in peritoneal dialysis peritonitis is a uncommon complication which requires prompt evaluation to rule out mesenteric ischemia as it carries a high mortality and its management will be surgical.

보건소 건강증진사업 수행과정의 질 평가

서영준,정애숙,박태선,김주경,박남수,이희원 韓國保健敎育.健康增進學會 2003 보건교육건강증진학회지 Vol.20 No.1

This study purports to assess the process quality of health promotion programs at public health centers. The sample used in this study consisted of 242 public health centers in South Korea. Data was collected with self-administered questionnaires between September. 3rd to September. 23rd in 2002. The 95 returned questionnaires were analyzed by SPSS 11.0 version. The major findings are as follows; There is a need to improve (1) a procedure for reviewing and analyzing strategic planning, (2) the quality of the staff in charge of program management, (3) the function of planning and training for monitoring and evaluation, (4) the ability of program managers in advising and consulting with clients, and (5) a procedure for organizing resources and information. In conclusion, in order to improve the process quality of health promotion programs at public health centers, integrated support from various institutions such as public health centers, community, regional and national health authorities and administrative departments is required.

효소면역측정법을 이용한 Fumonisin의 검출법 개발

손동화,한성민,임선희,이인원,조선희,강신영,이경애 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

Fumonisin에 대한 효소면역측정법(ELISA)을 개발하기 위하여 특이항체를 생산하고, 분석조건을 확립하여, 인위적으로 오염시킨 옥수수 시료의 분석을 행하였다. Fumonisin B_1 (FB_1)을 cholera toxin (CT)에 결합시킨 FB_1-CT conjugate를 면역원으로 하여 Freund's adjuvant와 함께, 또는 단독으로 두 군의 토끼에 면역하고 특이항체를 생산하였다. 항혈청을 ELISA로 분석한 결과 그 adjuvant와 함께 면역한 경우 항체역가(titer)가 높게 나타났다. 가장 높은 항체역가(1 : 16,000)를 나타낸 항혈청 및 그로부터 정제한 항체를 이용하여 간접법 및 직접법에 의한 경합적 ELISA(ciELISA 및 cdELISA)를 각각 확립하였다. 이 항체의 유사독소와의 교차반응을 ciELISA로 조사하였을 때, FB_3에 대한 교차반응율은 2%로 매우 낮았으나, FB_2에 대한 것은 179%로 다소 높았다. FB_1의 검출한계는 ciELISA에서 0.03ppb, cdELISA에서 0.3 ppb로 각각 나타났다. 실제 곡물시료의 분석에 ELISA 활용가능성을 조사하기 위하여 인위적으로 FB_1을 오염시킨 옥수수를 75% methanol로 추출하고 그 회수율을 측정한 결과, 분석이 불안정하여 회수율이 일정하게 나타나지 않았다. 그러나 방해물질의 제거를 위하여 시료추출액을 strong anion exchange(SAX) cartridge를 거쳐 세척한 다음 분석하였을 때, 3~10 ppm 범위의 FB_1 오염 옥수수에서 평균 34%(CV의 평균, 8.2%)의 안정된 회수율을 보였다. In order to develop enzyme-linked immunosorbent assay (ELISA) for fumonisins, production of specific antibodies, establishement of ELISA conditions, and quantitation of the toxin from spiked corns by ELISA were performed. Fumonisin B_1 (FB_1) conjugated to cholera toxin (CT) with or without Freund's adjuvant was subcutaneously injected into 2 groups of rabbits. When the titer of the antisera produced by each rabbit tested, higher titer was observed in case of the immunization with the adjuvant. By use of the antiserum showing the highest titer (1 : 16,000) and its purified antibodies, competitive indirect and direct ELISA's (ciELISA and cdELISA) were established, respectively. When the cross-reactivity of the antibody against fumonisin analogs was investigate by the ciELISA is was very low against B_3 (2%) but high against fumonisin B_2 (179%). The sensitivity of the ELISAs was also very high, because the detection limit for FB_1 was 0.03 ppb in ciELISA and 0.3 ppb in cdELISA. When the ELISA's were applied to the spiked corns after extraction with 75% methanol, the assay recovery of FB_1 was too unstable to assay. However, when cleanup by strong anion exchange (SAX) cartridge was introduced to remove interfering materials, the mean ELISA recovery of FB_1 from corns spiked to 3~10 ppm was found to be 34.0% and stable (mean of CV, 8.2%).

Composite membranes of poly(lactic acid) with zinc-added bioactive glass as a guiding matrix for osteogenic differentiation of bone marrow mesenchymal stem cells.

Oh, Sun-Ae,Won, Jong-Eun,Kim, Hae-Won Technomic Pub 2012 Journal of biomaterials applications Vol.27 No.4

<P>This study aims to produce a degradable and bone-bioactive membrane for guiding bone regeneration by combining a degradable polymer, poly(lactic acid) (PLA), with a bioactive inorganic zinc-containing bioactive glass (ZnBG). The in?vitro osteogenic development of rat bone marrow mesenchymal stem cells (rBMSCs) upon different membrane substrates (pure PLA control, PLA-BG, and PLA-ZnBG) was investigated in terms of bone cell phenotype syntheses and mineralization. Results showed significantly stimulated production of alkaline phosphatase and osteocalcin at days 14 and 21 in the membranes containing BG and ZnBG, with more in the samples containing ZnBG. The addition of ZnBG in PLA allowed the rBMSCs to express a high level of bone sialoprotein as confirmed by immunostaining. Cellular mineralization of the secreted extracellular matrix showed a significantly higher Ca level on the BG- and ZnBG-added membrane than on the PLA, and the more so in the ZnBG-added one. Based on the in?vitro assessments using rBMSCs, the ZnBG-added PLA is considered to be of potential use in guiding active bone regeneration within the periodontal pocket.</P>

인간 배아줄기세포 배양을 위한 영양세포의 최적밀도에 관한 연구

윤보애 ( Bo Ae Yoon ),설혜원 ( Hyun Won Seol ),백진아 ( Jin Ah Baek ),서진 ( Jin Seo ),정주원 ( Ju Won Jung ),김희선 ( Hee Sun Kim ),오선경 ( Sun Kyung Oh ),문신용 ( Shin Yong Moon ),최영민 ( Young Min Choi ) 서울大學校 醫學硏究院 人口醫學硏究所 2009 人口醫學硏究論集 Vol.22 No.-

정상과 비정상핵형을 가진 인간 배아줄기세포의 비교 분석

설혜원 ( Hye Won Seol ),백진아 ( Jin Ah Baek ),정주원 ( Ju Won Jung ),윤보애 ( Bo Ae Yoon ),김희선 ( Hee Sun Kim ),오선경 ( Sun Kyung Oh ),구승엽 ( Seung Yup Ku ),김석현 ( Seok Hyun Kim ),문신용 ( Shin Yong Moon ),최영민 ( Young M 서울대학교 인구의학연구소 2011 人口醫學硏究論集 Vol.24 No.-

Human embryonic stem cells (hESCs) are a useful source for biological research fields and applications in regenerative medicine. The maintenance and distribution of the undifferentiated hESCs of normal karyotypes are very important. The changes of colony morphology were observed during the culturing of hESCs, and chromosomal analysis was performed in this study. Abnormal karyotypes were identified in the cells which have the changes of the colony morphology. Attachment ratio and the spontaneous differentiation ratio of abnormal cells were compared to those of normal cells. High cell attachment ratio and low spontaneous differentiation ratio were confirmed in karyotypically abnormal cells. HESCs showed chromosomal anomaly if the colony morphology changed, and high attachment ratio and low spontaneous differentiation ratio have been observed during the cell culture. Our results suggest that the morphology of the colonies would play a ro1e as a criterion for chromosomal anomaly.

모유영양아와 인공영양아의 이환율 비교

김미원(Mi Won Kim),신희선(Hee Sun Shin),김정선(Jeong Sun Kim),안채순(Choi Soon Ahn),오상은(Sang Eun Oh),유경원(Kyoung Won Yu),이애란(Ae Ran Lee),장영숙(Young Sook Jang) 여성건강간호학회 1993 모자간호학회지 Vol.3 No.2