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Das, Subhasish,Teja, K. Charan,Mukherjee, Sandip,Seal, Soma,Sah, Rajesh Kumar,Duary, Buddhadeb,Kim, Ki-Hyun,Bhattacharya, Satya Sundar Elsevier 2018 Environmental research Vol.161 No.-
<P><B>Abstract</B></P> <P>Carlinoside is a unique compound well-known for its excellent curative potential in hepatitis. There is a substantial research gap regarding the medicinal use of carlinoside, as its concentrations are greatly variable (depending on locality). We cultivated <I>Cajanus cajan</I> using vermicompost as a major organic amendment at two locations (Sonitpur and Birbhum) with different soil types, but identical climate conditions. Sonitpur soils were richer in soil organic C (SOC), enzyme activation, and N/P content than Birbhum. However, vermi-treatment improved many soil properties (bulk density, water retention, pH, N/P/K, and enzyme activity) to narrow the locational gap in soil quality by 15–28%. We also recorded a many-fold increment in SOC storage capacities in both locations, which was significantly correlated with carlinoside, total phenol, and flavonoid contents in <I>Cajanus</I> leaves. This significantly up-regulated the carlinoside induced expression of the bilirubin-solubilizing UGT1A1enzyme in HepG2 cell and rat liver. Leaf extracts of vermicompost-aided plants could cure hepatitis in affected rat livers and in the HepG2 cell line. Accordingly, vermi-treatment is an effective route for the growth of <I>Cajanus</I> as a cash crop for biomedical applications and can produce a concurrent improvement in soil quality.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Soil organic C primarily induces carlinoside biosynthesis in <I>Cajanus cajan</I>. </LI> <LI> Vermicompost elevates the medicinal value of <I>Cajanus cajan</I> against hepatitis. </LI> <LI> Vermi-treated <I>Cajanus</I> yield carlinoside stably irrespective of growing location. </LI> <LI> Vermi-treated leaf extracts expedited bilirubin solubilization in rat liver. </LI> </UL> </P>
Subhasish Das,Asis Mazumdar 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.7
This research provides experimental outcomes of hydrodynamic flow formations around two square piers of same widthpositioned in inline front and eccentric rear arrangement with a constant longitudinal spacing and eccentricity. Measurements ofinstantaneous velocities in polar coordinate system were taken by a Doppler type acoustic velocimeter. The distributions and contourprofiles of time mean tangential, radial and vertical velocities, kinetic energy and intensities due to turbulence are presented at fiveazimuth angles 0°, -45°, 45°, -90° and 90° in vertical planes. From time mean velocity fields, velocity vectors were determined andutilised to explain added flow features. Horseshoe vortices vorticity and the strength (circulation) are resolved by usingcomputational hydrodynamics method. The preset outcomes are compared with previous researches where cylindrical and triangularshaped piers were used at same experimental conditions. The arrangement of square piers and the interfering among wake andhorseshoe vortices for inline-front and eccentric-rear piers, respectively - take part in a major role for making and forming biggerdepth of scour near eccentrically positioned rear pier. Due to bluff body existence, friction drag becomes less effective than thepressure drag. Horseshoe vortex strength for eccentric-rear pier is observed greater than strength for inline-front pier.
Dutta, Tanushree,Kim, Ki-Hyun,Uchimiya, Minori,Kumar, Pawan,Das, Subhasish,Bhattacharya, Satya Sundar,Szulejko, Jan Elsevier 2016 Environmental research Vol.151 No.-
<P><B>Abstract</B></P> <P>Large-scale assemblies of people in a confined space can exert significant impacts on the local air chemistry due to human emissions of volatile organics. Variations of air-quality in such small scale can be studied by quantifying fingerprint volatile organic compounds (VOCs) such as acetone, toluene, and isoprene produced during concerts, movie screenings, and sport events (like the Olympics and the World Cup). This review summarizes the extent of VOC accumulation resulting from a large population in a confined area or in a small open area during sporting and other recreational activities. Apart from VOCs emitted directly from human bodies (e.g., perspiration and exhaled breath), those released indirectly from other related sources (e.g., smoking, waste disposal, discharge of food-waste, and use of personal-care products) are also discussed. Although direct and indirect emissions of VOCs from human may constitute <1% of the global atmospheric VOCs budget, unique spatiotemporal variations in VOCs species within a confined space can have unforeseen impacts on the local atmosphere to lead to acute human exposure to harmful pollutants.</P>
Gazi, Md. Amran,Mahmud, Sultan,Fahim, Shah Mohammad,Kibria, Mohammad Golam,Palit, Parag,Islam, Md. Rezaul,Rashid, Humaira,Das, Subhasish,Mahfuz, Mustafa,Ahmeed, Tahmeed Korea Genome Organization 2018 Genomics & informatics Vol.16 No.4
Shigella spp. constitutes some of the key pathogens responsible for the global burden of diarrhoeal disease. With over 164 million reported cases per annum, shigellosis accounts for 1.1 million deaths each year. Majority of these cases occur among the children of the developing nations and the emergence of multi-drug resistance Shigella strains in clinical isolates demands the development of better/new drugs against this pathogen. The genome of Shigella flexneri was extensively analyzed and found 4,362 proteins among which the functions of 674 proteins, termed as hypothetical proteins (HPs) had not been previously elucidated. Amino acid sequences of all these 674 HPs were studied and the functions of a total of 39 HPs have been assigned with high level of confidence. Here we have utilized a combination of the latest versions of databases to assign the precise function of HPs for which no experimental information is available. These HPs were found to belong to various classes of proteins such as enzymes, binding proteins, signal transducers, lipoprotein, transporters, virulence and other proteins. Evaluation of the performance of the various computational tools conducted using receiver operating characteristic curve analysis and a resoundingly high average accuracy of 93.6% were obtained. Our comprehensive analysis will help to gain greater understanding for the development of many novel potential therapeutic interventions to defeat Shigella infection.
Md. Amran Gazi,Sultan Mahmud,Shah Mohammad Fahim,Mohammad Golam Kibria,Parag Palit,Md. Rezaul Islam,Humaira Rashid,Subhasish Das,Mustafa Mahfuz,Tahmeed Ahmeed 한국유전체학회 2018 Genomics & informatics Vol.16 No.4
Shigella spp. constitutes some of the key pathogens responsible for the global burden of diarrhoeal disease. With over 164 million reported cases per annum, shigellosis accounts for 1.1 million deaths each year. Majority of these cases occur among the children of the developing nations and the emergence of multi-drug resistance Shigella strains in clinical isolates demands the development of better/new drugs against this pathogen. The genome of Shigella flexneri was extensively analyzed and found 4,362 proteins among which the functions of 674 proteins, termed as hypothetical proteins (HPs) had not been previously elucidated. Amino acid sequences of all these 674 HPs were studied and the functions of a total of 39 HPs have been assigned with high level of confidence. Here we have utilized a combination of the latest versions of databases to assign the precise function of HPs for which no experimental information is available. These HPs were found to belong to various classes of proteins such as enzymes, binding proteins, signal transducers, lipoprotein, transporters, virulence and other proteins. Evaluation of the performance of the various computational tools conducted using receiver operating characteristic curve analysis and a resoundingly high average accuracy of 93.6% were obtained. Our comprehensive analysis will help to gain greater understanding for the development of many novel potential therapeutic interventions to defeat Shigella infection.