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- 저자 : Subburaj, Saminathan (검색결과 35 건)
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Subburaj, T.,Prasanna, K.,Kim, Ki Jae,Ilango, P. Robert,Jo, Yong Nam,Lee, Chang Woo Elsevier 2015 Journal of Power Sources Vol.280 No.-
<P><B>Abstract</B></P> <P>Micro-sized Li<SUB>4</SUB>Ti<SUB>5−x</SUB>Bi<SUB>x</SUB>O<SUB>12</SUB> (0 ≤ x ≤ 0.15) materials are synthesized using a simple solid state method in air. The structural, morphological, and electrochemical characteristics of Bi-doped lithium titanates and pristine samples are methodically analyzed by X-ray diffraction (XRD), Raman spectroscopy, field emission-scanning electron microscopy (FE-SEM), and electrochemical impedance spectroscopy (EIS). The XRD and Raman spectroscopy results demonstrate that bismuth-doping do not alter the spinel structure and good crystalline materials are synthesized. The FE-SEM images show that all samples possess the same morphological characteristics, with a particle size distribution of 0.5–1 μm. The electrochemical cycling testing reveals that the Li<SUB>4</SUB>Ti<SUB>4.9</SUB>Bi<SUB>0.10</SUB>O<SUB>12</SUB> sample exhibits discharge capacities of 205.4 mAh g<SUP>−1</SUP>, 160.8 mAh g<SUP>−1</SUP>, and 135.4 mAh g<SUP>−1</SUP> after 50 cycles at 1C, 5C, and 10C-rates, respectively. The differential capacity curves suggest that the Li<SUB>4</SUB>Ti<SUB>4.9</SUB>Bi<SUB>0.10</SUB>O<SUB>12</SUB> sample has a weaker polarization effect than the other samples. The EIS measurements imply that the Li<SUB>4</SUB>Ti<SUB>4.9</SUB>Bi<SUB>0.10</SUB>O<SUB>12</SUB> sample possesses a high electronic conductivity and lithium ion diffusivity, which demonstrate that this new Li<SUB>4</SUB>Ti<SUB>4.9</SUB>Bi<SUB>0.10</SUB>O<SUB>12</SUB> material would be a good candidate as an anode for lithium ion batteries.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bi-doped Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> anode materials are synthesized by facile solid-state method. </LI> <LI> Bi-doping leads to give high electronic conductivity and lithium diffusivity. </LI> <LI> Also, it is effective to be in stable structure and suppression of polarization effect. </LI> <LI> Li<SUB>4</SUB>Ti<SUB>4.9</SUB>Bi<SUB>0.10</SUB>O<SUB>12</SUB> material would be a good candidate as an anode for lithium ion batteries. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Subburaj, Saminathan,Jeon, Yongsam,Tu, Luhua,Jin, Yong-Tae,Kumari, Shipra,Lee, Geung-Joo Nijhoff/Junk 2018 Plant growth regulation Vol.86 No.1
<P>Long non-coding RNAs (lncRNAs) are a class of RNA regulatory molecules having roles in wide range of biological processes. They have been demonstrated to regulate gene expression at the posttranscriptional and transcriptional levels and to function in stress responses in plants and animals, but nothing is known about lncRNAs in Camelina (Camelina sativa L.), an emerging oil crop. Here, we report the first prediction of lncRNAs in the Camelina genome using comprehensive genomic approaches. We examined a Camelina drought stress cDNA library, and 5390 candidate Camelina sativa lncRNAs (CsalncRNAs) were identified, including 670 sense, 692 antisense, 1347 intergenic, and 2681 intronic harboring CsalncRNAs. The identified CsalncRNAs had an average nucleotide (nt) length of 497 bp and were mapped on each chromosome of C. sativa. Functional characterization through gene ontology (GO) and GO motif (GOMo) analysis of neighboring protein coding (PC) genes and motifs in the intergenic CsalncRNAs, respectively, indicated that these CsalncRNAs were involved in transcription-related activity, proteins, DNA and RNA binding, and abiotic/biotic stress response. Approximately 4.6% of CsalncRNA sequences were masked as repeat elements enriched with many repetitive sequences of transposable elements (TE), indicating the involvement of transposon silencing. Additionally, 55 intergenic CsalncRNAs were predicted as targets of miRNA, whereas nine target mimics were identified. Expression profiling of seven randomly selected CsalncRNAs using real-time quantitative polymerase chain reaction (RT-qPCR) showed tissue-specific expression, and these were highly up-regulated in Camelina leaves under extreme drought. Results of expression profiling indicated that these CsalncRNAs are involved in the progression of Camelina growth and development as well as its response to drought stress. Our results provide a basis for the functional study of lncRNAs in C. sativa that will serve as a valuable resource for future studies of the regulatory mechanisms underlying its growth and development as well as its stress response.</P>
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Voxel-Based Thickness Analysis of Intricate Objects
Subburaj, K.,Patil, Sandeep,Ravi, B. Society for Computational Design and Engineering 2006 International Journal of CAD/CAM Vol.6 No.1
Thickness is a commonly used parameter in product design and manufacture. Its intuitive definition as the smallest dimension of a cross-section or the minimum distance between two opposite surfaces is ambiguous for intricate solids, and there is very little reported work in automatic computation of thickness. We present three generic definitions of thickness: interior thickness of points inside an object, exterior thickness for points on the object surface, and radiographic thickness along a view direction. Methods for computing and displaying the respective thickness values are also presented. The internal thickness distribution is obtained by peeling or successive skin removal, eventually revealing the object skeleton (similar to medial axis transformation). Another method involves radiographic scanning along a viewing direction, with minimum, maximum and total thickness options, displayed on the surface of the object. The algorithms have been implemented using an efficient voxel based representation that can handle up to one billion voxels (1000 per axis), coupled with a near-real time display scheme that uses a look-up table based on voxel neighborhood configurations. Three different types of intricate objects: industrial (press cylinder casting), sculpture (Ganesha idol), and medical (pelvic bone) were used for successfully testing the algorithms. The results are found to be useful for early evaluation of manufacturability and other lifecycle considerations.
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Subburaj, T.,Jo, Y.N.,Lee, C.W. Elsevier 2014 CURRENT APPLIED PHYSICS Vol.14 No.8
The conventional electrolyte system has been compared with the ionic liquid (IL) additive containing electrolyte system at room temperature as well as elevated temperature. In this work, two types of monocationic ILs such as 1-butyl-3-methylpyrrolidinium hexafluorophosphate (Pyr IL) and 1-ethyl-3-methylimidazolium hexafluorophosphate (IMI IL) are added as an additive at two different weight ratios in 1.15 M LiPF<SUB>6</SUB> (EC/EMC = 3/7 v/v) electrolyte solution, the structural, electrochemical and thermal characteristics of LiNi<SUB>0.80</SUB>Co<SUB>0.15</SUB>Al<SUB>0.05</SUB>O<SUB>2</SUB> (NCA)/carbon full-cell in different electrolyte formulations have been reconnoitered. X-ray diffraction (XRD) studies have proved that IL as an electrolyte additive does not alter the structural stability of cathode materials after cycling. Under room temperature, Pyr IL additives at 1 wt% and 3 wt% deliver better cycleability than others, with the retention ratios of 93.62% and 92.8%, respectively. At elevated temperature, only 1 wt% Pyr IL additive is giving stable capacity retention ratio of 80.74%. Ionic conductivity and self-extinguishing time (SET) values are increasing with respect to the amount of additive added to the electrolyte. Thermal studies reveal that 3 wt% Pyr IL is favorable regarding the safety of the battery as it shows shifting of peak to higher temperature of 272.10 <SUP>o</SUP>C. Among the IL additives evaluated in this study, addition of 1 wt% Pyr IL is the most desirable additive for achieving the best cycling performance as well as thermal behavior of Li-ion batteries.
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Subburaj, Saminathan,Chung, Sung Jin,Lee, Choongil,Ryu, Seuk-Min,Kim, Duk Hyoung,Kim, Jin-Soo,Bae, Sangsu,Lee, Geung-Joo Springer International 2016 Plant cell reports Vol. No.
<P>Site-directed mutagenesis of nitrate reductase genes using direct delivery of purified Cas9 protein preassembled with guide RNA produces mutations efficiently in Petunia x hybrida protoplast system. The clustered, regularly interspaced, short palindromic repeat (CRISPR)-CRISPR associated endonuclease 9 (CRISPR/Cas9) system has been recently announced as a powerful molecular breeding tool for site-directed mutagenesis in higher plants. Here, we report a site-directed mutagenesis method targeting Petunia nitrate reductase (NR) gene locus. This method could create mutations efficiently using direct delivery of purified Cas9 protein and single guide RNA (sgRNA) into protoplast cells. After transient introduction of RNA-guided endonuclease (RGEN) ribonucleoproteins (RNPs) with different sgRNAs targeting NR genes, mutagenesis at the targeted loci was detected by T7E1 assay and confirmed by targeted deep sequencing. T7E1 assay showed that RGEN RNPs induced site-specific mutations at frequencies ranging from 2.4 to 21 % at four different sites (NR1, 2, 4 and 6) in the PhNR gene locus with average mutation efficiency of 14.9 +/- 2.2 %. Targeted deep DNA sequencing revealed mutation rates of 5.3-17.8 % with average mutation rate of 11.5 +/- 2 % at the same NR gene target sites in DNA fragments of analyzed protoplast transfectants. Further analysis from targeted deep sequencing showed that the average ratio of deletion to insertion produced collectively by the four NR-RGEN target sites (NR1, 2, 4, and 6) was about 63:37. Our results demonstrated that direct delivery of RGEN RNPs into protoplast cells of Petunia can be exploited as an efficient tool for site-directed mutagenesis of genes or genome editing in plant systems.</P>
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Saminathan Subburaj,하혜정,진용태,전용삼,Luhua Tu,김진백,강시용,이긍주 한국식물학회 2017 Journal of Plant Biology Vol.60 No.2
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that regulate gene expression at posttranscriptional level. They might regulate tolerance to abiotic stress. However, this has not been studied in Tradescantia (BNL clone 3340), an important bio-indicator for measuring the effect of radiation. Up to date, gamma irradiation (γ-IR) responsive miRNAs and their expressions patterns in plants in response to γ-IR stress have not been reported yet. Therefore, putative γ-IR responsive miRNAs from a cDNA library of Tradescantia after radiation stress were predicted in this study by using comprehensive genomic approaches. Here, we identified 37 miRNAs belonged to 36 different miRNA families. The quantitative real-time polymerase chain reaction (qRT-PCR) analysis of five randomly selected miRNAs were confirmed that their responsiveness to γ-IR stress. Target prediction revealed that 37 miRNAs targeted 149 genes involved in stress tolerance regulation, light response, redox systems, signaling pathways, DNA repair, and transcription factors. A coordinated reverse expression changes between miRNAs and their target genes further validated that these predicted miRNAs could be γ-IR responsive and likely to be directly involved in stress regulatory networks of Tradescantia. Altogether, these results will provide novel insights to the understanding of molecular mechanisms of miRNAs involved in plant response to γ-IR.
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