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김삼용,노흥규,신영태,윤성열,이헌영,전병숙 대한핵의학회 1983 핵의학 분자영상 Vol.17 No.1
Fifteen cases of Korean hemorrhagic fever who were admitted Chungnam National University Hospital from October 1981 to December 1981 were analysed on the evaluation of metabolic changes of the thyroid hormones, and thyroid function status in each clinical phase. 1) Serum T₃, T₄ concentration, FT₄I and T₄/TBG ratio were significantly lower (p〈0.001, p〈0.005, P〈0.005, P〈0.001, respectively) than control group in oliguric and early diuretic phase of Korean hemorrhagic fever. With the recovery of illness, abnormal thyroid hormones and thyroid function indices tend to become normal range. But Serum FT₄, TSH anlE TBG concentration were within normal range in all phase of KHF. Thus in Korean hemorrhagic fever, euthyroidism is probably maintained by normal or elevated serum FT₄. 2) T₄/T₃ and rT₃/T₃ rato (p〈0.005, p〈0.005) were increased in oliguric and early diuretic phase of MHF. These results might be explained by decreased peripheral conversion of T₄ to T_3 in oliguric and early diuretic phase.
Jeon, Woo Young,Yoon, Byoung Hoon,Ko, Byoung Sam,Shim, Woo Yong,Kim, Jung Hoe Springer-Verlag 2012 Bioprocess and biosystems engineering Vol.35 No.1
<P>Xylose reductase (XR) is the first enzyme in <SMALL>D</SMALL>-xylose metabolism, catalyzing the reduction of <SMALL>D</SMALL>-xylose to xylitol. Formation of XR in the yeast <I>Candida tropicalis</I> is significantly repressed in cells grown on medium that contains glucose as carbon and energy source, because of the repressive effect of glucose. This is one reason why glucose is not a suitable co-substrate for cell growth in industrial xylitol production. XR from the ascomycete <I>Neurospora crassa</I> (NcXR) has high catalytic efficiency; however, NcXR is not expressed in <I>C</I>. <I>tropicalis</I> because of difference in codon usage between the two species. In this study, NcXR codons were changed to those preferred in <I>C</I>. <I>tropicalis</I>. This codon-optimized NcXR gene (termed NXRG) was placed under control of a constitutive glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter derived from <I>C</I>. <I>tropicalis</I>, and integrated into the genome of xylitol dehydrogenase gene (<I>XYL2</I>)-disrupted <I>C</I>. <I>tropicalis</I>. High expression level of NXRG was confirmed by determining XR activity in cells grown on glucose medium. The resulting recombinant strain, LNG2, showed high XR activity (2.86 U (mg of protein)<SUP>−1</SUP>), whereas parent strain BSXDH-3 showed no activity. In xylitol fermentation using glucose as a co-substrate with xylose, LNG2 showed xylitol production rate 1.44 g L<SUP>−1</SUP> h<SUP>−1</SUP> and xylitol yield of 96% at 44 h, which were 73 and 62%, respectively, higher than corresponding values for BSXDH-3 (rate 0.83 g L<SUP>−1</SUP> h<SUP>−1</SUP>; yield 59%).</P>
Jeon, Joong-Kyu,Moon, Han-Young,Ann, Ki-Yong,Kim, Hong-Sam,Kim, Yang-Bea Korea Concrete Institute 2006 International Journal of Concrete Structures and M Vol.18 No.e2
In this study, the effect of supplementary materials(GGBS, PFA, SF) on sulfuric acid corrosion resistance was assessed by measuring the compressive strength, corroded depth and weight change at 7, 28, 56, 91, 180 and 250 days of immersion in sulfuric acid solution with the pH of 0.5, 1.0, 2.0 and 3.0. Then, it was found that an increase in the duration of immersion and a decrease in the pH, as expected, resulted in a more severe corrosion irrespective of binders: increased corroded depth and weight change, and lowered the compressive strength. 60% GGBS mortar specimen was the most resistant to acid corrosion in terms of the corroded depth, weight change and compressive strength, due to the latent hydraulic characteristics and lower portion of calcium hydroxide. The order of resistance to acid was 60% GGBS>20% PFA>10% SF>OPC. In a microscopic examination, it was found that acid corrosion of cement matrix produced gypsum, as a result of decomposition of hydration products, which may loose the structure of cement matrix, thereby leading to a remarkable decrease of concrete properties.
Gain Characteristics of Multi-output LLC Resonant Converter
Jae-Sam Lee,Dong-Young Huh,Sung-In Kang,Eun-Soo Kim,Sang-Ho Jang,Yong-Seog Jeon 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5
This paper describes the gain characteristics of a multi-output LLC series resonant converter by using the new analytical method. Specially, using the Math-CAD simulated result, this paper analyzes an influence from the secondary leakage inductance of transformer. The theoretical results are verified through an experimental prototype of the 430W 3-output LLC resonant converter for 46inch PDP power module.
Molecular cloning and analysis of the <i>Thermus caldophilus</i> ADP-glucose pyrophosphorylase
Kim, Yong-Sam,Sohn, Hosung,Jin, Un-Ho,Suh, Seok-Jong,Lee, Sang Chul,Jeon, Jae Heung,Lee, Dae-Sil,Kim, Cheorl-Ho,Ko, Jeong Heon IPC Science and Technology Press 2007 Enzyme and microbial technology Vol.41 No.4
<P><B>Abstract</B></P><P>Previously, we have purified a highly thermostable ADP-glucose pyrophosphorylase (EC 2.7.7.27; AGPase) from <I>Thermus caldophilus</I> GK-24. In the present paper, we further report the molecular cloning and characterization of the thermostable bacterial AGPase. Using a 0.6-kb DNA probe obtained by polymerase chain reaction (PCR) with a primer set deduced from the <I>N</I>-terminal and internal amino acid sequence, the AGPase gene was cloned. The cloned AGPase gene had a 1245-bp open reading frame that encodes a protein of 414 amino acids. Then, the full-length AGPase gene was further cloned into the pHCE19T(II) vector and was expressed in <I>Escherichia coli</I> DH5α. Western analysis of the recombinant enzyme showed the same immunity as the wild-type enzyme with a molecular mass of ca. 46kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The kinetic parameters of the recombinant AGPase were basically similar to the wild type. The <I>N</I>-terminal region of <I>T. caldophilus</I> AGPase showed a partial similarity to that of <I>E. coli,</I> and R336 and P295 were identical to those of <I>E. coli</I> AGPase. In addition, sequence comparison revealed that R177 and P235 of <I>T. caldophilus</I> AGPase were aligned with K195 and K247 of <I>E. coli,</I> and K376 with R386, while K195 residue of <I>E. coli</I> was reported to be located in the glucose-1-phosphate (Glc-1-P) substrate binding region.</P>
Enhanced Cytotoxicity of 5-FU by bFGF through Up-Regulation of Uridine Phosphorylase 1
Young-Sam Im,Hea Kyeong Shin,Hye-Ryun Kim,So-Hee Jeong,김승렬,Yong-Min Kim,Do Hyung Lee,Seong-Ho Jeon,이현우,최중국 한국분자세포생물학회 2009 Molecules and cells Vol.28 No.2
Anti cancer agent 5-FU (Fluoro Uracil) is a prodrug that can be metabolized and then activated to interfere with RNA and DNA homeostasis. However, the majority of administered 5-FU is known to be catabolized áå=îáîç in the liver where Dihydropyrimidine dehydrogenase (DPD) is abundantly expressed to degrade 5-FU. The biological factors that correlate with the response to 5-FU-based chemotherapy have been proposed to include uridine phosphorylase (UPP), thymidine phosphorylase (TPP), p53 and microsatellite instability. Among these, the expression of UPP is known to be controlled by cytokines such as TNF-α, IL1 and IFN-γ. Our preliminary study using a DNA microarray technique showed that basic fibroblast growth factor (bFGF) markedly induced the expression of UPP1 at the transcription level. In the present study, we investigated whether bFGF could modulate the expression of UPP1 in osteo-lineage cells and examined the sensitivity of these cells to 5-FU mediated apoptosis.
강삼석(Sam-Seok Kang),김윤경(Yoon-Kyeong Kim),조광식(Kwang-Sik Cho),정상복(Sang-Bouk Jeong),황해성(Hea Seong Hwang),김명수(Myung-Su Kim),신일섭(Il-Sheob Shin),신용억(Yong-Uk Shin),원경호(Kyeong-Ho Won),최장전(Jang-Jeon Choi) 한국원예학회 2011 원예과학기술지 Vol.29 No.6
"슈퍼골드" 품종은 농촌진흥청 국립원예특작과학원 배시험장에서 1994년에 "추황배"의 품질을 개선하기 위하여 "추황배"에 "만풍배"를 교배하였다. 2002년에 1차 선발하고 2008년에 최종선발하였다. 화분친인 "만풍배"와 같이 수세는 강하고, 수자는 반개장성이다. 꽃이 풍부하고, 꽃가루도 많아 수분수로 이용할 수 있다. "슈퍼골드"는 검은무늬병(Alternaria kikuchiana)에 포장 저항성을 나타내었다. 수확기는 9월 11일 전후로 황금배보다 5일 빨리 수확할 수 있다. 과형은 편원형이며 과피색은 수확기에 녹백색이다. 평균과중 570g이고, 당도는 13.6°Brix이다. 육질은 부드럽고 과즙이 풍부하여 식미가 우수하다. 저온 보구력은 6개월 정도이다. 자가불화합 유전자형을 확인하기 위해 유전자형이 알려진 품종의 꽃가루로 교배를 한 결과 28.8%로 낮은 착과율을 보인 이십세기 조합을 제외한 모든 조합에서 64.5%에서 91.0%로 비교적 높은 착과를 보였다. "조옥", "장십랑", "이십세기"와의 교배에서 화분관 선단이 이상비대하는 현상을 보였지만 교배에 이용한 모든 화분친 품종과 교배화합성을 보였다. PCR-RFLP 결과 ‘슈퍼골드’의 자가불화합 유전자형은 S3S4로 확인되었다. Pear cultivar "Supergold" (Pyrus pyrifolia var. culta Nakai) was originated from the cross between "Chuwhangbae" and "Manpungbae" with the aims of improving the fruit quality of "Chuwhangbae" cultivar at Pear Research Station of National Institute of Horticultural & Herbal Science, Rural Development Administration in 1994. "Supergold" was preliminarily selected in 2002 and named in 2008. The tree shows a vigorous growth habit and semi-spread characters like as "Manpungbae". Furthermore, it has sufficient flowers and carries abundant pollen grains, so it can also be used as a pollinator. "Supergold" is highly resistant to black leaf spot (Alternaria kikuchiana) in the field condition. The optimum harvest time is around Sep. 11th, which is ahead of "Whangkeumbae" about 5 days in the harvest period. The fruit shape is oblate and fruit skin color is greenish-white at harvesting time. The average weight of fruit is 570 g, and the soluble solids content is 13.6 °Brix. The flesh is very soft and juicy, and renders good eating quality. Shelf life is about 6 months under the cold storage condition. To determine the self-incompatibility (SI) genotype of "Supergold" pear cultivar, it was crossed with other cultivars of which SI genotypes have already known. The result of cross-pollinations of "Supergold" with other cultivars showed relatively high rates of fruit set from 64.5% to 91.0%, except for the cross with pollens of "Nijisseiki" that represented only 28.8% of fruiting rate. Although sometimes the stigma of "Supergold" crossed with "Hayatama", "Chojuro", and "Nijisseiki" showed malformed pollen tube tips, "Supergold" is generally supposed to have cross-compatibility with all other pollen donor cultivars. It is considered that the S-allele of "Supergold" is S3S4, which is based on the result of PCR-RFLP.