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Mouse Leukemia 세포로부터 분리한 방사능 감수성 변이주의 90 kDa Protein 결손
백순영,전내광,산본수,택전소삼 ( Soon Young Paik,Hiroshi Tauchi,Osamu Yamamoto,Shozo Sawada ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.2
Wild type murine leukemia L5178Y and its radiation-sensitive mutants, M10 and LX830 cells were compared for their sensitivity to radiation, methyl methanesulfonate (MMS) and 4-nitroquinoline-l-oxide (4-NQO). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) pattern of the two extremely radiation-sensitive mutants compared with that of L5178Y showed that a 90-kDa protein was deficient. This deficiency probably indicates that M10 and LX830 may have a common defect in their ability to repair radiation-induced damage.
Establishment of a Stable Cell Line Expressing Human BMP2/7-PTD for Efficient Osteogenic Induction
Seung-Won Park(박승원),Seok-Woo Kang(강석우),Tae-Won Goo(구태원),Seong Ryul Kim(김성렬),Soon-Young Paik(백순영) 한국생명과학회 2012 생명과학회지 Vol.22 No.4
유전자 재조합 이형이합체 인간 뼈 형성촉진인자(rhBMP)들은 뼈 재생을 위한 조직공학연구에 중요한 요소들이다. 그러나 실제 뼈 재생에 관한 연구를 수행함에 이들을 이용하는 것은 뼈 형성 촉진인자들이 짧은 반감기를 가지며 비교적 고농도의 단백질을 사용해야 하기 때문에 그만큼 많은 비용이 소요된다는 문제점을 가지고 있다. 이러한 한계를 뛰어넘기 위하여, 본 연구에서는 단백질 전달 서열(PTD)이 융합된 인간 뼈 형성촉진인자 2와 7이 이형이합체 유전자 재조합 단백질(rhBMP2/7-PTD)을 발현하는 세포주를 확립하였다. 이형이합체의 형성은 BMP 2와 BMP 7 단백질 및 PTD 영역의 사이에 각각 4개의 glycine 아미노산 염기서열이 첨가될 수 있도록 하여 각 단백질의 folding이 자유롭도록 디자인하였다. 이렇게 개발된 세포주는 고농도의 rhBMP2/7-PTD을 지속적으로 발현하여 배양액 내로 분비함으로 조직공학용 연구 및 개발에 효율적으로 이용 할 수 있다. 이상의 세포주에서 발현된 rhBMP2/7-PTD 단백질은 뼈 세포분화 유도를 확인 할 수 있는 ALP 활성을 나타냄으로써 뼈 성장촉진 단백질로서 생물학적인 활성을 가지고 있음을 보였다. 본 연구의 결과로 개발된 rhBMP2/7-PTD 형질전환 세포주는 향후 뼈 조직 재생과 같은 연구에 중요하고 효과적인 도구로 이용될 수 있을 것으로 사료된다. Heterodimeric recombinant human bone morphogenetic proteins (rhBMPs) are powerful tools for bone tissue engineering. However, BMPs have several important limitations in their application to bone regeneration. BMPs have a short half-life and must be used in high concentrations, which may be cost-inefficient. To overcome these problems, we established a stable cell line that expressed the fusion protein comprised of recombinant human BMP2/7 heterodimer protein and PTD (rhBMP2/7-PTD). This stable cell line enabled high process yields by continuously expressing rhBMP2/7-PTD products at high levels throughout cultivation. This synthesized BMP7 was fused to a BMP2 protein with four glycine residues (to allow free bond rotation of the domains) and PTD. To demonstrate that the rhBMP2/7-PTD protein that was secreted from an rhBMP2/7-PTD-expressing stable cell line exhibited biological activity consistent with its role as an osteogenic differentiation induction growth factor, we evaluated BMP-induced ALP activity. Our results suggest that this cell line may be a powerful and efficient tool for applications such as bone tissue regeneration.
B형 간염 바이러스의 만성 감염 및 수직 감염 예방과 HLA 대립유전자와의 연관성
김종현(Jong-Hyun Kim),표철우(Chul-Woo Pyo),허성숙(Seong-Suk Hur),김양겸(Yang-Kyum Kim),고대균(Dae-Kyun Koh),오진희(Jin-Hee Oh),허재균(Jae-Kyun Hur),강진한(Jin-Han Kang),백순영(Soon-Young Paik),유문간(Mun-Gan Rhyu),김금용(Gum-Ryong Ki 대한미생물학회 2003 Journal of Bacteriology and Virology Vol.33 No.3
Streptomyces lividasn 의 Blasticidin S 에 대한 내성 Mechanism
백순영,야촌정,미호이수하라,마사노리수기야마 생화학분자생물학회 1995 BMB Reports Vol.24 No.3
A mechanism of resistance to blasticidin S in Streptomyces lividans 66 was investigated. Although the microorganism was highly resistant to the drug in vivo, the in vitro protein-synthesizing system was significantly sensitive. The possible resistance mechanism was discussed and suggested.
The Use of Recombinant DNA Techniques to Study Sterol Metabolism in Streptomyces sp.
Paik, Soon-Young,Shozo, Sawada,Yang, Han-Chul 한국생화학분자생물학회 1990 생화학분자생물학회 소식 Vol.10 No.3
The nucleotide sequence of the cholesterol oxidase gene (choA) of Streptomyces sp. was determined previously. We searched for the promoter region of the choA gene and found a strong promoter at about 1.3-kb upstream from choA gene. Thus we sequenced the fragment between the promoter and choA gene and found a new open reading frame (ORF), unexpectedly. Furthermore, the results of transcript analysis suggested that the c h A gene was transcribed polycistronically under the control of the promoter. In this review, we also discuss homologies in the new ORF and its activity of cytochrome P-450.
杉山政則,白舜英,能美良作 한국산업미생물학회 1985 한국미생물·생명공학회지 Vol.13 No.1
Much of the progress in genetic engineering has been accomplished by employing Escherichia coli as the host organism. For many reasons, however, some other organisms have greater potential as alternatives to E. coli. In particular, streptomycetes are attractive organisms as hosts especially for the producation of various secondary metabolites such as antibiotics. In this article, therefore, we reviewed the techniques for development of vector system and expression of genes for antibiotic biosynthesis in streptomycete hosts.