워크스테이션 클러스터에서의 비동기 반복법의 구현

박필성,신순철 수원대학교 자연과학연구소 2000 자연과학논문집 Vol.3 No.-

The main stream of parallel programming today is using synchronous iterative algorithms, and workload balance is a crucial factor in the design of parallel programs. However, it is difficult to achieve on heterogeneous workstation clusters where the available computing power of each processor is unpredictable. As a way to overcome such a problem, the idea of asynchronous iteration has grown out and is being increasingly used and studied. But most authors use the technique on shared memory systems, which is in some sense, easy to implement. We use the algorithm in the MPI(Message Passing Interface) environment on the workstation cluster Atom at University of Suwon, that consists of 7 nodes each of which has 2 Celeron CPUs. We reprot implementation details and numerical experiments of the asynchronous algorithm, together with that by usual synchronous algorithms.

Xylose isomerase 유전자를 클로닝한 알코올 발효성 효모의 개발 대장균에서 Xylose isomerase 의 증폭

박종성,이인구,박우철,박희동,홍순덕,박완 경북대학교 유전공학연구소 1987 遺傳工學硏究所報 Vol.2 No.1

It has been found that high levels of overproduction of D-xylose isomerase(D-xylose ketol isomerase, EC 5.3.1.5) can not be obtained by the cloning of intact D-xylose isomerase gene(xylA) on high copy number plasmids alone. So the 1.4Kb DNA fragment containing the promoterless xylA gene was isolated and cloned in + 0.1Kb region from lac promoter and in + 0.3Kb region from P_L promotor. The 1.4Kb DNA fragment contained the structural gene and ribosome binding site for D-xylose iomerase. E. coli transformants by these recombinant plasmids could produce Dxylose isomerase 6 times by lac promoter, and 15 times by P_L promoter as much as parent strain.

The novel anti-adipogenic effect and mechanisms of action of SGI-1776, a Pim-specific inhibitor, in 3T3-L1 adipocytes

PARK, YU-KYOUNG,HONG, VICTOR SUKBONG,LEE, TAE-YOON,LEE, JINHO,CHOI, JONG-SOON,PARK, DONG-SOON,PARK, GI-YOUNG,JANG, BYEONG-CHURL UNKNOWN 2016 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.37 No.1

<P>The proviral integration site for moloney murine leukemia virus (Pim) kinases, consisting of Pim-1, Pim-2 and Pim-3, belongs to a family of serine/threonine kinases that are involved in controlling cell growth and differentiation. Pim kinases are emerging as important mediators of adipocyte differentiation. SGI-1776,an inhibitor of Pim kinases, is widely used to assess the physiological roles of Pim kinases, particularly cell functions. In the present study, we examined the effects of SGI-1776 on adipogenesis. The anti-adipogenic effect of SGI-1776 was measured by Oil Red O staining and AdipoRed assays. The effect of SGI-1776 on the growth of 3T3-L1 adipocytes was determined by cell count analysis. The effects of SGI-1776 on the protein and mRNA expression of adipogenesisrelated proteins and adipokines in 3T3-L1 adipocytes were also evaluated by western blot analysis and RT-PCR, respectively. Notably, SGI-1776 markedly inhibited lipid accumulation during the differentiation of 3T3-L1 preadipocytes into adipocytes. On a mechanistic level, SGI-1776 inhibited not only the expression of CCAAT/enhancer-binding protein-alpha (C/EBP-alpha), peroxisome proliferator-activated receptor-gamma (PPAR-gamma) and fatty acid synthase (FAS), but also the phosphorylation of signal transducer and activator of transcription-3 (STAT-3). Moreover, SGI-1776 decreased the expression of adipokines, including the expression of leptin and regulated on activation, normal T cell expressed and secreted (RANTES) during adipocyte differentiation. These findings demonstrate that SGI-1776 inhibits adipogenesis by downregulating the expression and/or phosphorylation levels of C/EBP-alpha, PPAR-gamma, FAS and STAT-3.</P>

DHA and EPA Down-regulate COX-2 Expression through Suppression of NF-ՊB Activity in LPS-treated Human Umbilical Vein Endothelial Cells

Soon Ae Lee,Hye Jung Kim,Ki Churl Chang,Jong Chul Baek,Ji Kwon Park,Jeong Kyu Shin,Won Jun Choi,Jong Hak Lee,Won Young Paik 대한생리학회-대한약리학회 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.4

Inflammatory processes of vascular endothelial cells play a key role in the development ofatherosclerosis. We determined the anti-inflammatory effects and mechanisms of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on LPS-treated human umbilical vein endothelial cells (HUVECs) to evaluate their cardioprotective potential. Cells were pretreated with DHA, EPA, or troglitazone prior to activation with LPS. Expression of COX-2, prostaglandin E₂ (PGE₂) and IL-6 production, and NF-ՊB activity were measured by Western blot, ELISA, and luciferase activity, respectively. Results showed that EPA, DHA, or troglitazone significantly reduced COX-2 expression, NF-ՊB luciferase activity, and PGE₂ and IL-6 production in a dose-dependent fashion. Interestingly, low doses (10ՌM) of DHA and EPA, but not troglitozone, significantly increased the activity of NF-ՊB in resting HUVECs. Our study suggests that while DHA, EPA, and troglitazone may be protective on HUVECs under inflammatory conditions in a dose-dependent manner. However there may be some negative effects when the concentrations are abnormally low, even in normal endothelium.

Association Between Anther Culture Response and 1RS Chromosome Segment in wheat Cultivars (Triticum aestivum L.)

Soon Jong Kweon,Jong Nae Hyun,Jong Min Ko,Dong Soo Park,Shin Churl Bae,Duck Yong Suh,Huhn Pal Moon,Dal Ung Kim 한국육종학회 2003 한국육종학회지 Vol.35 No.1

To analyze the effect of 1RS chromosome segment on anther culture efficiency in wheat, 44 cultivars released from several countries were evaluated. The cultivars applied for this investigation came from several regions and it was necessary to confirm cult

癩患者 末梢血中 血淸內 Purine nucleoside phosphorylase 活性에 關한 硏究

朴美順,李承哲,田寅淇,金永杓 大韓癩學會 1987 癩學 會誌 Vol.20 No.1

Anti-Adipogenic Effects on 3T3-L1 Cells and Zebrafish by Tanshinone IIA

Park, Yu-Kyoung,Obiang-Obounou, Brice Wilfried,Lee, Jinho,Lee, Tae-Yun,Bae, Myung-Ae,Hwang, Kyu-Seok,Lee, Kyung-Bok,Choi, Jong-Soon,Jang, Byeong-Churl MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.10

<P>Tanshinone IIA is a diterpene quinone isolated from the roots of <I>Salvia miltiorrhiza</I> bunge that has traditionally been used in China for the treatment of cardiovascular and cerebrovascular disorders. Although there is recent evidence showing that tanshinone IIA has an anti-obesity effect, its underlying mechanism of anti-obesity effect is poorly understood. Here, we investigated the effect of tanshinone IIA on lipid accumulation in 3T3-L1 preadipocytes and zebrafish. Notably, tanshinone IIA at 10 μM concentration greatly reduced lipid accumulation and triglyceride (TG) contents during 3T3-L1 preadipocyte differentiation, suggesting its anti-adipogenic effect. On mechanistic levels, tanshinone IIA reduced the expression levels of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS), and perilipin A but also the phosphorylation levels of signal transducer and activator of transcription-3/5 (STAT-3/5) in differentiating 3T3-L1 cells. In addition, tanshinone IIA strongly inhibited leptin and resistin mRNA expression in differentiating 3T3-L1 cells. Importantly, the tanshinone IIA’s lipid-reducing effect was also seen in zebrafish. In sum, these findings demonstrate that tanshinone IIA has anti-adipogenic effects on 3T3-L1 cells and zebrafish, and its anti-adipogenic effect on 3T3-L1 cells is largely attributable to the reduced expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3/5.</P>

소아 상완골 외과 골절 치료 후 운반각 변화 및 골극 형성

박상원,이순혁,한승범,황인철 대한골절학회 2000 대한골절학회지 Vol.13 No.3

Purpose; The purpose of this study is to evaluate the bony union, change of carrying angle and spur formation after treatment of lateral condyle fractures in children. Materials and methods: From march 1990 to september 1998, 30 cases of lateral condylar fractures treated with percutaneous pinning or open reduction and internal fixation and followed up more than 1 years were classified by the fracture type(Milch) and degrees of displacement(Jakob). We analysed the bony union, change of carrying angle and spur formation according to the classifications. Results: The mean union time was 7weeks. Average change of carrying angle was 2.1 degree(varus: 17, valgus: 9, no change: 4). Change of carrying angle was not related to the type of fracture and degree of displacement(p$gt;0.05). Lateral spur formation was observed in 16 cases(Jakob I: 0, II: 6, III: 10 and Milch I: 2, II: 14). There was statistical significancy between the Jakob stage and lateral spur formation (p$lt;0.05) but not between Milch type(p$gt;0.05). Conclusion: The change of carrying angle was not affected by the fracture type and degree of displacement. Lateral spur formation was not affected by fracture type but related to the extent of displacement.

AZD1208, a pan‐Pim kinase inhibitor, inhibits adipogenesis and induces lipolysis in 3T3‐L1 adipocytes

Park, Yu‐,Kyoung,Obiang‐,Obounou, Brice Wilfried,Lee, Kyung‐,Bok,Choi, Jong‐,Soon,Jang, Byeong‐,Churl John Wiley and Sons Inc. 2018 JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Vol.22 No.4

<P><B>Abstract</B></P><P>The proviral integration moloney murine leukaemia virus (Pim) kinases, consisting of Pim‐1, Pim‐2 and Pim‐3, are involved in the control of cell growth, metabolism and differentiation. Pim kinases are emerging as important mediators of adipocyte differentiation. AZD1208 is a pan‐Pim kinase inhibitor and is known for its anti‐cancer activity. In this study, we investigated the effect of AZD1208 on adipogenesis and lipolysis in 3T3‐L1 cells, a murine preadipocyte cell line. AZD1208 markedly suppressed lipid accumulation and reduced triglyceride contents in differentiating 3T3‐L1 cells, suggesting the drug's anti‐adipogenic effect. On mechanistic levels, AZD1208 reduced not only the expressions of CCAAT/enhancer‐binding protein‐α (C/EBP‐α), peroxisome proliferator‐activated receptor‐γ (PPAR‐γ), fatty acid synthase (FAS), acetyl‐CoA carboxylase (ACC) and perilipin A but also the phosphorylation of signal transducer and activator of transcription‐3 (STAT‐3) in differentiating 3T3‐L1 cells. Remarkably, AZD1208 increased cAMP‐activated protein kinase (AMPK) and LKB‐1 phosphorylation while decreased intracellular ATP contents in differentiating 3T3‐L1 cells. Furthermore, in differentiated 3T3‐L1 adipocytes, AZD1208 also partially promoted lipolysis and enhanced the phosphorylation of hormone‐sensitive lipase (HSL), a key lipolytic enzyme, indicating the drug's HSL‐dependent lipolysis. In summary, the findings show that AZD1208 has anti‐adipogenic and lipolytic effects on 3T3‐L1 adipocytes. These effects are mediated by the expression and/or phosphorylation levels of C/EBP‐α, PPAR‐γ, FAS, ACC, perilipin A, STAT‐3, AMPK and HSL.</P>

주의력결핍과잉행동장애와 Alpha-1C-Adrenergic 수용체 유전자와의 연관성에 대한 연구

조수철,김재원,김붕년,황준원,박미라,김순애,조대연,유희정,정운선,손정우,박태원 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.1

Objectives : Neurobiological and pharmacological research has suggested that the dysregulation of the central noradrenergic systems might be involved in the pathophysiology of ADHD. The aim of this study was to examine the association of the alpha-1C-adrenergic receptor gene (ADRA1C) with ADHD in Korean subjects. Methods : In a case-control study, we assessed 186 DSM-IV ADHD probands and 150 normal controls. One hundred and eight trios were studied in a family-based association analysis. Psychiatric diagnoses were derived through structured diagnostic interviews. For the clinical evaluation of the ADHD subjects, the Child Behavior Checklist (CBCL), the ADHD Rating Scale-IV (ARS) and the Junior Temperament and Character Inventory (JTCI) were administered. A computerized continuous performance test (CPT) was used to measure the inattention and impulsivity of the ADHD children. Results : There were no significant differences in the genotype or allele frequencies of the ADRA1C PstI polymorphism between the ADHD and control group (p>0.05). The transmission disequilibrium test (TDT) analysis observed no evidence for biased transmission of any of the alleles of the PstI polymorphism. There were no significant differences in the CPT or JTCI profiles between those ADHD subjects with the CC genotype and those with the other (CT+TT) genotypes at the PstI polymorphism. Conclusion : The results of this study do not support the ADRA1C as a major genetic susceptibility factor in ADHD.