산소유리라디칼이 뇌조직 미크로좀분획의 Na⁺-K⁺-ATPase 활성도에 미치는 영향

오세문(Sae Moon Oh)손영숙(Young Sook Son),최길수(Kil Soo Choi),임정규(Jung Kyoo Lim),정명희(Myung Hee Chung) 대한약리학회 1982 대한약리학잡지 Vol.18 No.2

중추신경계의 혈관폐쇄 또는 충격손상에 의한 허혈병소에서 진행되는 병리적 변화에 산소유리라디칼이 중요한 역할을 할 것으로 시사되고 있다. 저자는 산소유리라디칼이 뇌조직에 미치는 영향 중 특히 신경세포 정지막전위 유지에 중요한 Na⁺-K⁺-ATPase의 활성도에 미치는 영향을 관찰하기 위하여 산틴산화효소 반응계와 뇌조직미크로좀을 이용하여 본 연구를 시행하였다. 미크로좀분획(microsomal fraction)을 산틴과 산틴산화효소와 함께 반응시켰을 때, 분획의 Na⁺-K⁺-ATPase의 활성도는 현저한 불활성화를 보인 반면, Mg⁺⁺-ATPase의 활성도는 별로 영향을 받지 않았다. 이 불활성화는 산틴과 신틴산화효소 중 어느 한 물질이라도 반응계에 존재하지 않는 경우에는 나타나지 않았고, 두 물질이 같이 반응계에 존재할 때 나타났다. 산틴과 산틴산화효소의 반응에서 생성되는 산소유리라디칼들 중, 어떤 것이 Na⁺-K⁺-ATPase불활성화에 관계하고 있는가를 알아보기 위하여, 산수유리라디칼 각각에 대하여 제독작용을 가진 효소나 화학물질을 사용하여 불활성화의 저해유무를 관찰하였다. O^-₂⋅의 제독효소인 superoxide dismutase, H₂O₂의 제독효소인 catalase와 ¹O₂의 제독물질인 1,4-diazabixyclo(2,2,2)octane을 각각 사용하였을 때, 이들 물질들이 농도에 비례하여 Na⁺-K⁺-ATPase의 불활성화가 저해되었다. 그러나 OH⋅의 제독물질인 mannitol은 뚜렷한 효과를 보이지 못했다. 이상의 결과는 산소유리라디칼들 중 O^-₂⋅, H₂O₂ 및 ¹O₂가 Na⁺-K⁺-ATPase의 불활성화에 관계하고 있고, OH⋅는 거의 관계하지 않는다는 것을 시사하여 주었다. 이로 미루어, 산소유리라디칼에 의한 뇌조직 Na⁺-K⁺-ATPase의 불활성화는 뇌 허혈병소에서 관찰되는 신경세로의 기능적 장해를 유발시키는 한 요인으로 사료되었다. The effects of xanthine-xanthine oxidase reaction on brain microsomal Na⁺-K⁺-ATPase activity were studied to see possible involvement of oxygen free radicals in pathologic change occurring in ischemic state of CNS accompanied by cerebral vascular occlusion or impact injury. When microsomal fraction was incubated with xanthine ana xanthine oxidase, Na⁺-K⁺-ATPase activity of the fraction was markedly inactivated (80% inactivation) whereas btssl Mg⁺⁺-ATPase was much less sensitive (less than 10% inactivation) compared to that of Na⁺-K⁺-ATPase. The inactivation was observed only in the presence of both xanthine and xanthine oxidase, not either of them alone, and the extent of inactivation was dependent on the concentration of xanthine. In an attempt to determine which of the oxygen species was responsible for the inactivation, the ability of various scavengers to overcome the inactivation was tested. Superoxide dismutase, catalase and 1,4-diazabicyclo(2,2,2)octane were shown to reverse the inactivation of the ATPase in dose-dependent manner. In contrast, mannitol as well as other OH⋅quenchers were ineffective in limiting oxygen radical-induced inactivation. Thus OO^-₂⋅, H₂O₂ and ¹O₂ were implicated to be mediators involved in the inactivation. Since oxygen radicals are suspected as being a cause of the peroxidative damaging process in train ischemia, the ATPase inactivation by oxygen radicals may be a possible contributing factor which gives rise to functional derangement of nerve cells observed in the pathologic process.

The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

생약제제인 이공산(異功散)의 Cisplatin 유도 신장독성 보호 및 항산화 효과

Lee, Kyung-Tae,Ahn, Kyoo-Seok,Chang, Sung-Goo,Oh, Soo-Myung,Jung, Jee-Chang 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung-Tae Lee, Kyoo-Seok Ahn¹, Sung-Goo Chang², Soo-Myung Oh²and Jee-Chang Jung²¹College of Pharmacy, ²Oriental Medicine and ³College of Medicine, and East-West Medical Reserch Institute, Kyung Hee University, Seoul, Korea. Preventive and Antioxidative Effects of Crude Drug Preparation(E-kong-san) on Cisplatin Induced Nephrotoxicity. Proceedings of International Symposium on East-West Medicine, Seoul. 201-211, 1999. -Nephroprotective effects of a crude drug-prearation (E-kong-san) were determined from cisplatin on rabbit kidney proximal tubule and human renal cortical cells by MTT assays and sustained glucose consumption on ciplatin-induced human renal cortical tissue. Levels of creatinine and blood ure nitrogen(BUN) in serum after administration of cisplatin(0.75mg/kg,i.p.) to E-kong-san(0.75g/kg/day,p.o.)-pretreated rats were markedly lower compared to those of cisplatin-treated rats. Moreover, the administration of E-kong-san significantly inhibited the loss of body weight of cisplatin injected rts. These findings suggest that E-kong-san on the cisplatin induced nephrotoxicity led us to investigate whether the effect of this water extract was a result of anti-oxidation. E-kong-san showed strong free radical scavenger activities on 1,1-dipheny1-2picrylhydrazil(DPPH) radical and xanthine/xanthine oxidase(XOD) generated superoxide anion radical(O2-). We further studied the effects of E-kong-san on lipid peroxidation in rat liver microsomes induced by enzymatic and nonenzymatic methods. E-kong-san exhibited significant inhibition on both ascorbic acid/Fe2+and ADP/NADPH/Fe3+ induced lipid peroxidation in rat liver microsomes. Based on these results, we suggest that-E-kong-san attenuate the cisplatin induced cytotoxicity and its mechanism can be eplained by antioxidant.

捻轉法이 竝行된 鍼刺戟 및 電鍼刺戟이 鎭痛效果에 미치는 影響

Park, Dong-Seok,Kim, Ji-Hoon,Min, Byung-Il,Oh, Soo-Myung 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Dong-Seok Park¹, Ji-Hoon Kim¹ and Byung-Il Min, Soo-Myung Oh,³¹Department of Acupuncture and Moxibustion, College of Medicine, ²Department of Physiology, College of Medicine, And ³East-West Medical Research Institute, Kyung Hee University,Seoul, Korea. Analgesic Effect of Manual Acupuncture and Electroacupuncture combined with Twirling Method. Proceeding of International Symposium on East-West Medicine Seoul. 187-200, 1999.-Twirling the needle (TN) is one of several needing methods frequently used for acupuncture in the oriental medicine. The present study was conducted to see if TN enhanced the antinociceptive effect produced by plain-or electroacupuncture in the rat tail flick test. Rats (Sprague-Dawley, 200-300g)lightly anesthetized with thiopental sodium(40mg/kg, i.p.) were used in the tail-flick test. The basal reaction time for tail-flick was 2 sec. For "plain" acupuncture(PA), a needle was inserted for 20 min into a Chock-samni (ST36) acupuncture point which (is located at the anterior tibial muscle and about 10mm below the knee joint). For electroacupuncture(EA), train-pulses(3 Hz,0.3-ms pulse width, 0.2-0.3mA) were applied to the inserted needle for the period of insertion. For combining TN with either PA or EA, TN was performed during the each acupuncture period. Two methods for TN were used; twirling 3 times for 1 second persisted for 1 min in every 5 mins(long-duration and long-interval;LDLI) and twirling 3 times for 1 second persisted for 10 sec in every 1 min(short-duration and short-interval;SDSI). PA and EA produced an increase in tail flick latency (TFL); peak increases were 21.3±5.1% and 42.1±17.3% of the pre-acupunture control, respectively, and occurred immediately after cessation of 20min-acupuncture. Performing TN combined with PA increased TFL more than PA itself by showing a greater peak increase in TFL when performed with a SDSI-TN than with a LDLI-TN(60.6±12.5% and 42.7±22.7% of the pre-acupuncture control, respectively). Performing TN combined with EA also increased TFL more than EA itself and a greater peak increase in TFL was observed with a SDSI-TN as compared to a LDLI-TN(77.5±13.8% and 67.3±14.0% of the pre-acupuncture control, respectively). These results indicate that TN enhances both PA-and EA-produced antinociception where an greater enhanced antinociception than a LDLI-TN. It is suggested that performing a SDSI-TN combined with EA seems to be one of the most effective ways to produce analgesia.

감귤류 종자 추출물의 N-nitrosodimethylamine 생성억제에 관한 연구

오혁수,안용석,나임순,오명철,오창경,김수현 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.5

The effect of inhibition on N-nitrosodimethylamine (NDMA) formation by methanol extracts from 6 kinds of citrus seed (Citrus sunki, Citrus natsudaidai, Citrus suleata, Citrus tangerina, Citrus grandis and Citrus obovoidea) were investigated. The contents of moisture, crude protein, crude fat, carbohydrate, ash, flavonoid and totat phenol in citrus seeds were 4~6, l1~15, 32~46, 22~45, 2~4%, 12~24mg% and 53~133㎎%, respectively. The solid contents and yield of citrus seed extracts were 0.8~1.0 and 0.7~1.1%, respectively. Nitrite-scavenging activity by methanol extracts from citrus seeds increased with increasing extract dosage. Furthermore, the nitrite scavenging activity was pH dependent being the highest at pH 1.2 (42.7~96.9%) and the lowest at pH 6.0 (19.9~62.6%). Scavenging effects of nitrite by reaction time showed high effects under 3hr reaction time. The inhibition effect of NDMA formation by the citrus seed was strengthened as the amount of extract increased. The inhibition rate of methanol extracts from citrus seeds on NDMA formation showed 1.2~39.8%, 21.3~60.1% and 47.4~94.0%, according to add 1, 3 and 5㎖, respectively. Therefore, the inhibition effect of NDMA formation by the citrus seed was strengthened as the concentration of extract increased.

중소기업에서의 ERP의 성공적인 도입을 위한 요소와 주요 ERP패키지의 특성에 대한 조사연구

吳根泰,金明洙 水原大學校 1999 論文集 Vol.17 No.-

ERP is the general trend of industrial information system and many companies are introducing strategically to strengthen the competitive power. But it is too expensive for small companion to introduce and there are frequent occasions which are far from satisfactory because of making a bad choice. So this study proposes the critical success factors for the introduction of ERP system to small companies which is extracted from many success and failure cases and shows the characteristics and functions of main ERP packages.

토마토 엽 절편으로부터 식물체 재분화

오명규,이영만,박문수 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

조직배양에 의한 토마토의 효율적인 재분화 체계를 밝히고저 토마토 엽절편을 재료로 하여 배지 조성 및 품종간의 차이가 재분화에 미치는 영향을 조사하였다. 토마토 엽절편으로 부터 유기된 shoot수는 Calypso에서 27∼79개로 PI 134417에서의 1∼30개에 비하여 현저히 높았으며, MS배지에 IAA 0.02 ㎎/ℓ와 zeatin 2.0㎎/ℓ가 첨가된 배지에서 Calypso가 79개, PI 134417이 30개로 가장 양호하였다. 토마토의 재분화식물체 shoot의 발근은 MS기본배지에 IAA 0.02 ㎎/ℓ+ GA₃ 0.05㎎/ℓ+ AgNO₃ 10㎎/ℓ를 첨가한 배지가 40%로 가장 양호하였다. Effects of culture medium on the plant regeneration of tomato (Lycopersicon esculentum L.) using leaf segments were investigated to establish the efficient in vitro regeneration systems. Numbers of shoot differentiated from leaf explants were remarkably higher cv. Calypso of 275079 compared to that observed in PI 134417 of 1 to 30. Among tested culture media, MS medium supplimented with 2.0 ㎎/ℓ zeatin and 0.02㎎/ℓ IAA was the best resulting in with 79 shoots for cv. Calypso and 30 shoots for PI 134417. Among rooting media tested, MS basal medium added with 0.02㎎/ℓIAA, 0.05㎎/ℓ GA₃, and 10.0㎎/ℓ AgNO₃ gave rise to the best rooting frequency in vitro tomato roots.

비용 제약하에서의 소모성 동시조달부품의 최적구매량 결정

吳根泰,金明洙 水原大學校 1998 論文集 Vol.16 No.-

In this paper we consider the CSP requirement determination problem of new equipment(machine) system. For the newly procured equipment systems, mathematical analyses are made for the system which is constructed with the consumable parts to derive the associated CSP requirement determination model in mathematical expression. Based on these on these analyses, a mathematical model is derived for making an optimal CSP requirement determination subject to the constraint of satisfying any given fund limitation. We assume that the failure of a part follows a Poisson process. Firstly, the operational availability concept in CSP is defined and the relation between the general system availability and the operational availability concept in CSP is defined and the relation between the general system availability and the operational availability is established. Secondly, the problem is formulated as the operational availability maximization problem that should satisfy the fund limitation, and then, using the generalized Lagrange multipliers method, the optimal solution procedure is derived.

만성 틱장애와 뚜렛 증후군 아동과 청소년에 있어서의 리스페리돈 치료 효과 및 안정성에 관한 연구:임상 개방 연구

오은영,이명수,이정은 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.2

Objective : The purpose of this trial was to investigate the short-term and long-term safety and efficacy of risperidone in the treatment of chronic tic disorders and Tourette's syndrome in children and adolescents. Method Twenty-seven patients(24males, 3 females/12 Tourette's syndrome, 15 chronic motor or vocal tic disorders) participated a 16 week open-label trial. The ranges of age of patients were from 6 to 18 years. Clinical responses are measured by the Yale Global Tic Severity Scale(YGTSS). The patients were seen at baseline and for three follow up visits(4-week, 8-week, 16-week). Risperidone was started at 0.5mg/day and increased by 0.5mg/day every 5 days until a satisfactory level was reached or the patient complained of intolerable side effect. Results : 1) Clinical response revealed a statistically significant reduction on tic scores ranging from 35.37 to 22.14(37.4%) 2) Statistically significant improvement of tic symptoms appears in 4 weeks. There was statistically significant reduction on YGTSS scores in 8 weeks in comparison with 4 weeks, bur there was no statlstically significant reduction in 16 weeks in comparison with 8 weeks although there also was clinical improvement. 3) Doses of risperidone at the end of the trial ranged from 1mg to 3mg(mean = 2.02mg/day). 4) The most frequent side effects were sedation(13 cases, 48.1%), easy fatigue(4 cases, 14.8%), dry mouth(2 cases, 7.4%), urinary incontinence(1 case, 3.7%), constipation(1 case, 3.7%) and there was no extrapyramidal side effects. Conclusion : Risperidone, a neuroleptic with both serotonin and dopamine-blocking properties, appears to be effective in reducing tic frequency and intensity. This open clinical trial suggests that low-dose risperidone may be a promising alternative to conventional medications used for treating the symptoms of Tourettes syndrome and chronic tic diisorders in Korean children and adolescents.

肉類의 焙燒 방법에 따른 N-nitrosamine 含量에 關한 硏究

오명철,오창경,김수현 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

소갈비, 돼지갈비 및 통닭을 가스렌지와 숯불로 焙燒한 후 배소 전후의 N-nitrosamine과 그의 전구물질인 TMAO, TMA, DMA, nitrate 및 nitrite의 함량을 측정하였다. TMAO-N, TMA-N 및 DMA-N은 배소 전후 모든 시험구에서 뚜렷한 변화가 없었으며, 그 함량은 각각 N.D∼0.76 mg/kg, N.D∼2.57mg/kg 및 0.02∼0.60mg/kg 범위였다. 배소 후 nitrate-N는 감소하였고, nitrite-N는 증가하였으며, 그 함량은 각각 4.0∼6.7mg/kg 및 0.7∼3.2mg/kg 범위였다. N-nitrosamine은 배소 후에 NDMA가 5.9∼20.9 ㎍/kg, NDEA가 0∼3.0㎍/kg으로 검출되었다. The contents of N-nitrosmines and its precursors such as TMAO-N, TMA-N, DMA-N, nitrite-N and nitrate-N after broiling of beef-ribs, pork-ribs and chicken upon gas range and charcoal fire were investigated. The contents of TMAO-N, TMA-N and DMA-N no changed in all tested meats before and after broiling, and were in the range of N.D∼0.77mg/kg, N.D∼2.57mg/kg and 0.02∼0.60mg/kg, respectively. Nitrate-N discreased and Nitrite-N increased after broiling, and the contents were in the range of 4.0∼6.7mg/kg and 0.7∼3.2mg/kg, respectively. N-nitrosamines detected NDMA and NDEA in the broiled meats, and the contents were in the range of 5.9∼20.9㎍/kg and 0∼3.0㎍/kg respectively.