Enhanced antitumor immunotherapeutic effect of B-cell-based vaccine transduced with modified adenoviral vector containing type 35 fiber structures

Kim, E-K,Seo, H-S,Chae, M-J,Jeon, I-S,Song, B-Y,Park, Y-J,Ahn, H M,Yun, C-O,Kang, C-Y Macmillan Publishers Limited 2014 Gene therapy Vol.21 No.1

For successful clinical tumor immunotherapy outcomes, strong immune responses against tumor antigens must be generated. Cell-based vaccines compromise one strategy with which to induce appropriate strong immune responses. Previously, we established a natural killer T-cell (NKT) ligand-loaded, adenoviral vector-transduced B-cell-based anticancer cellular vaccine. To enhance tumor antigen delivery to B cells, we established a modified adenoviral vector (Ad-k35) that encoded a truncated form of the breast cancer antigen Her2/neu (Ad-k35HM) in which fiber structure was substituted with adenovirus serotype 35. We observed increased tumor antigen expression with Ad-k35HM in both human and murine B cells. In addition, an Ad-k35HM-transduced B-cell vaccine elicited strong antigen-specific cellular and humoral immune responses that were further enhanced with the additional loading of soluble NKT ligand KBC009. An Ad-k35HM-transduced, KBC009-loaded B-cell vaccine efficiently suppressed the in vivo growth of established tumors in a mouse model. Moreover, the vaccine elicited human leukocyte antigen (HLA)-A2 epitope-specific cytotoxic T-cell responses in B6.Cg (CB)-Tg (HLA-A/H2-D) 2Enge/Jat mice. These findings indicated that the Ad-k35 could be appropriate for the preclinical and clinical development of B-cell-based anticancer immunotherapies.

Anti-inflammatory activity of SMP30 modulates NF-κB through protein tyrosine kinase/phosphatase balance

Jung, K. J.,Lee, E. K.,Kim, S. J.,Song, C. W.,Maruyama, N.,Ishigami, A.,Kim, N. D.,Im, D. S.,Yu, B. P.,Chung, H. Y. Springer Science + Business Media 2015 Journal of molecular medicine Vol.93 No.3

<P>Recent studies on senescence marker protein-30 (SMP30) have shown that it has an important functional role in the aging process, but its precise participation in cellular works has not been fully determined. We hypothesize that SMP30 plays crucial roles in signaling processes by modulating the balance of protein tyrosine kinase (PTK)/protein tyrosine phosphatase (PTP) and in activating proinflammatory NF-kappa B. An experimental paradigm of gain and loss of SMP30 function was established using SMP30-overexpressed YPEN-1 cells (herein referred to as 'SMP30(+) cells') and SMP30 (Y/-) knockout mouse kidneys. The resulting data show that SMP30 expression suppressed oxidative stress-induced PTK/PTP dysregulation and PP1/2A inactivation in SMP30(+) cells, leading to the suppression of NF-kappa B activation. In the kidneys of SMP30 (Y/-) mice, SMP30 deficiency was found to induce NF-kappa B activation via the upstream signaling of NIK/IKK and MAPKs and to upregulate downstream NF-kappa B-responsive gene expression. In this study, we also demonstrate for the first time that SMP30 deficiency induced PTK activity in SMP30 (Y/-) kidneys, thereby significantly increasing the tyrosine phosphorylation of a catalytic subunit of PP2A (PP2Ac-Tyr307). Based on these findings, we propose that SMP30 involves NF-kappa B regulation through the PTK/PTP balance and that the age-related decrease of SMP30 causes NF-kappa B activation, which contributes to an exacerbation of the inflammatory process during aging. Key message SMP30-deficient mice induced a shorter lifespan and redox changes. Overexpression of SMP30 prevented oxidative stress insults. The depletion of SMP30 increased redox-related PTK/PTP imbalance and PP1/PP2A inactivation. The depletion of SMP30 caused an elevation of NF-kappa B-responsive inflammatory markers. SMP30 may be a potent inhibitory protein against oxidative stress and chronic inflammation.</P>

Up regulation of GW112 Gene by NFκB promotes an antiapoptotic property in gastric cancer cells

Kim, Kee K.,Park, Key S.,Song, Seok B.,Kim, Kyoon E. Wiley Subscription Services, Inc., A Wiley Company 2010 Molecular carcinogenesis Vol.49 No.3

<P>To clarify the regulatory mechanism of GW112 gene expression, 5′-flanking region of the human GW112 gene was isolated and characterized in the present study. 5′-RACE analysis showed a single transcription start site, which is located 142 nucleotides upstream of the translation initiation site. Transient transfection studies with serial deletion constructs and close examination of the sequences identified a putative NFκB binding sequence between −442 and −430, which could be responsible for efficient expression of the GW112 gene. Indeed, GW112 gene was found to be regulated by NFκB signals including overexpressed p65 and IκBα, IKK inhibitor, and proteasome inhibitor. Binding of NFκB to its putative site was confirmed by EMSA and ChIP assays. These results suggest that NFκB is an essential regulatory factor for GW112 transcription. Based on this finding, we next confirmed that inhibition of GW112 expression could induce apoptosis in the presence of cytotoxic agent in gastric cancer cells. Furthermore, knocking-down or overexpression of GW112 gene in gastric cancer cells demonstrated that GW112 has an antiapoptotic property against the cytotoxic agents-induced apoptosis. Taken together, these results suggest that GW112 could be an important mediator in NFκB-dependent tumorigenesis of digestive tract tissues. © 2009 Wiley-Liss, Inc.</P>

대두박을 기초로 한 잉어사료내 미생물 Phytase 의 첨가가 잉어의 성장 및 인 배설량에 미치는 영향

김광석(K . S . Kim),김정대(J . D . Kim),송재성(J . S . Song),정관식(K . S . Jeong),원창희(C . H . Won),우영배(Y . B . Woo) 한국영양사료학회 1996 韓國營養飼料學會誌 Vol.20 No.2

닭의 동품종내 계통간 잡종강세 이용시험

박상문,김동곤,송기덕,오봉국 한국축산학회 1965 한국축산학회지 Vol.7 No.1

To induce hybrid vigor by the reciprocal crossing with SWL (Sung Whan Line), MWL (Minnesota Line), AWL (Dembro Line) and BWL (Derby Line) of single comb white leghorn, 1,330 hens were divided into 16 blacks (Diallal cross with four Line) and fed under condition of N.R.C. feeding standard. In this experiment, hatchability, fertility, mortality, body weight, days required on up to first egg laying date, egg weight, egg quality, winter pauses, intensity, feed utilization, number of eggs layed during the testing period of days and brooding were investigated. Cross breeds showed a little higher fertility and hatchability but there were no significance when their parents had high hatchability. Cross breeds M♀×A♂, M♀×E♂ and A♀×S♂ showed more than ½ decrease in mortality, however cross bred hen showed 2.27% more decrease in mortality than purebreeds. Cross breeds of 6 blocks among 16 blocks at 6 weeks showed significant (P$lt;0.01) difference in body weight. Heaviest cross breed among all blocks were S♀×A♂ and A♀×S♂. Generally adult cross breeds showed heaviest body weight; especially in S♀×A♂ and A♀×S♂ cross breeds showed heaviest body weight among them. Dates requiring on up to first egg laying (50% laying date in the blocks) were showen at cross breeds. Cross breeds shorten 16.42 days than purebreeds and A♀×M♂ was showed shortest clay (171.0 days) in all cross breeds. ♀M×A♂ (175.0 days) and M♀×B♂ (175.0 days) were excellent. Cross breed showed increased egg weight as follows : A♀×S♂ 56.0 g, B♀×M♂ 56.62 g B♀×A♂ 57.40 g and there was significant (P$lt;0.05) difference in egg weight between pure breed and cross breed (M♀×S♂ and B♀×A♂), However, their egg weight was same one as standard (56.0 g) Generally productive block showed light egg weight, because egg weight are related to egg production, Those eggs didn't show any progress in thickness, meat and blood spot. Winter pauses became more short and intensity was more higher than purebreeds. They produced more eggs during winter than purebreeds. Feed utilization was very high in cross breeds. than in purebreeds. The number of egg at 500 days testing period (Hen house) was M♀×B♂ : 201,08, S♀×B♂ : 200.73, B♀×S♂ : 200.21, M♀×S♂ : 197.28 and M♀×A♂ : 191.69 and number of egg per hen in the block was from 230 to 256 in a year. SWL and BWL cross was very excellent in any condition. Generally, we could say that the number of egg was increased by crossing and it showed at 1% level of significance, but cross breeds increased broodiness about 0.56% than did in pure breeds.

Early Regulation of Viral Infection Reduces Inflammation and Rescues Mx-positive Mice from Lethal avian Influenza Infection

Song, M.S.,Cho, Y.H.,Park, S.J.,Pascua, P.N.Q.,Baek, Y.H.,Kwon, H.I.,Lee, O.J.,Kong, B.W.,Kim, H.,Shin, E.C.,Kim, C.J.,Choi, Y.K. American Association of Pathologists and Bacteriol 2013 The American journal of pathology Vol.182 No.4

Differing sensitivity of influenza A viruses to antiviral effects of the Myxovirus resistance (Mx) protein implies varying global gene expression profiles in the host. The role of Mx protein during lethal avian influenza (AI) virus infection was examined using Mx1-deficient C57BL/6 (B6-Mx1<SUP>-/-</SUP>) and congenic Mx1-expressing (B6-Mx1<SUP>+/+</SUP>) mice infected with a virulent, mouse-adapted avian H5N2 Ab/Korea/ma81/07 (Av/ma81) virus. After infection, B6-Mx1<SUP>+/+</SUP> mice were completely protected from lethal AI-induced mortality, and exhibited attenuated clinical disease and reduced viral titers and pathology in the lungs, compared with B6-Mx1<SUP>-/-</SUP> mice. Transcriptional profiling of lung tissues revealed that most of the genes up-regulated after infection are involved in activation of the immune response and host defense. Notably, more abundant and sustained expression of cytokine/chemokine genes was observed up to 3 dpi in B6-Mx1<SUP>-/-</SUP> mice, and this was associated with excessive induction of cytokines and chemokines. Consequently, massive infiltration of macrophages/monocytes and granulocytes into lung resulted in severe viral pneumonia and potentially contributed to decreased survival of B6-Mx1<SUP>-/-</SUP> mice. Taken together, our data show that dysregulated gene transcriptional activity corresponded to persistent induction of cytokine/chemokines and recruitment of cytokine-producing cells that promote inflammation in B6-Mx1<SUP>-/-</SUP> mouse lungs. Thus, we provide additional evidence of the interplay of genetic, molecular, and cellular correlates governed by the Mx1 protein that critically determine disease outcome during lethal AI virus infection.

Partial conductivities of mixed conducting BaCe_0.65Zr_0.2Y_0.15O_3–δ

Lim, D.-K.,Park, C.-J.,Choi, M.-B.,Park, C.-N.,Song, S.-J. Elsevier 2010 International journal of hydrogen energy Vol.35 No.19

<P><B>Abstract</B></P><P>The electrical properties of BaCe<SUB>0.65</SUB>Zr<SUB>0.2</SUB>Y<SUB>0.15</SUB>O<SUB>3–δ</SUB> (BCZY) were studied as a function of both oxygen partial pressure and water vapor partial pressure in the temperature range of 500–800 °C, and the partial conductivities of protons, holes, and oxygen vacancies were calculated from the defect model. P-type conduction was dominant in an oxidative atmosphere. In a wet atmosphere, BCZY was a mixed conductor of protons, holes, and oxygen ions. A conduction transition from protons to holes and/or oxygen ions was found with increasing temperature. The calculated activation energy of oxygen ion transport was 0.71 eV. The standard solution enthalpy for water dissolution was best fitted with a slope of −120.19 kJ/mol, which is somewhat smaller in absolute terms than that of BaCe<SUB>0.9</SUB>Y<SUB>0.1</SUB>O<SUB>3–δ</SUB> and of BaCe<SUB>0.8</SUB>Y<SUB>0.2</SUB>O<SUB>3–δ</SUB>. This result also agrees well with the literature reports that the Ba, rather than Sr, occupation of A-site and the Ce, rather than Zr, occupation of B-site in perovskite proton conductors induce more negative hydration enthalpies due to the increasing basicity of the corresponding oxides.</P>

Cloning of the Structural Gene for Hepatitis B Virus Surface Antigen into a Yeast Vector

김경태,송기방,최영철,이상기,한문희,Kim, K.T.,Song, K.B.,Choi, Y.C.,Rhee, S.K.,Han, M.H. 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.2

B형간염 virus의 표면항원(HBsAg) 유전자를 alcohol dehydrogenase (ADCI) 유전자의 promoter를 갖고 있으며 Escherichia coli와 효모인 Saccharomyces cerevisiae에서 자기복제할 수 있는 shuttle vector pAAR 6에 cloning하였다. 표면항원 유전자가 cloning된 재조합 plasmid를 S. cerevisiae SHY 3, YNN 27, D 13, ATCC 38517, ATCC 42677 등의 균주에 형질전환시켜 배양한 후 이들 균주로부터 virus의 표면항원이 생산됨을 방사능 면역측정법으로 확인하였다. 사용한 균주중 YNN 27주에서 가장 많은 표면항원 단백질이 생산되었고 이를 sucrose gradient centrifugation, CsCl density gradient 및 전자현미경으로 조사한 결과 간염환자의 혈청으로부터 분리한 22 nm 크기의 표면항원입자와 형태와 크기면에서 유사한 물질임이 확인되었다. The structural gene of hepatitis B virus surface antigen (HBsAg) was cloned into a shuttle vector pAAR 6 which is capable of autonomous replication and selection in both of the yeast Saccharomyces cerevisiae and Escherichia coli. This expression vector employs the 5'-flanking region of ADC I gene as a promoter in order for transcribing viral surface antigen coding sequences. After transformation of the recombinant plasmid into yeast strains such as SHY 3, YNN 27, D 13, ATCC 38517 and ATCC 42677, the expression of HBsAg gene in the host cells was evident. The protein synthesized in yeast cells was found to be similar in size, density and shape to the 22 nm particles isolated from the plasma of human hepatitis carriers.

Normal Zone Initiation and Propagation Characteristics of a Solid Nitrogen Cooled GdBCO Racetrack Pancake Coil

Song, J. B.,Kim, K. L.,Yang, D. G.,Jang, J. Y.,Ko, T. K.,Ahn, M. C.,Lee, H. G. IEEE 2012 IEEE transactions on applied superconductivity Vol.22 No.3

<P>This paper presents quench test results, of both experiment and simulation, of a racetrack pancake coil wound with a GdBCO-coated conductor tape in a solid nitrogen (SN2) cooling system at 58 K. The longitudinal normal zone propagation velocities in the curved section were 2 times faster than those in the straight section. Therefore, the straight section should be protected more than the curved section when using racetrack-type, GdBCO pancake coils in large-scale rotating machines. The simulated results showed good agreement with the experimentally observed normal zone initiation times.</P>

Nd: YAG의 자기적 성질에 관한 연구

송기봉,김장환,이재헌 부산대학교 1983 자연과학논문집 Vol.36 No.-

Magnetic susceptibilities of neodymium dopped yttrium aluminum garnet(Nd: YAG) were measured by Faraday method over a temperature range of 100K to 350K. Nd:YAG showed the characteristics of paramagnetism as expected. Obtained Nd-dopant concentration of this samples 1.8±0.2 at% by comparing experimental results to the Curie law. Molar diamagnetic susceptibility of YAG is found to be-(14±4)×10^(-5) cgs from the data.