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Nonactin hinders intracellular glycosylation in virus-infected baby hamster kidney cells.
Lee, Jin-Man,Kim, Jong-Guk,Kim, Tae-Ho,Lee, Do S,Kim, Jae H,Cho, Somi K,Riu, Key Z,Lee, Dong-Sun,Lee, Sang-Han D. A. Spandidos 2010 MOLECULAR MEDICINE REPORTS Vol.3 No.1
<P>Potent antiviral agents hinder virus-infected cell machinery, leading to rescue from viral damage. In this study, we aimed to identify selective intracellular glycosylation inhibitor(s) that do not suppress glycoprotein synthesis. Our results showed that nonactin is a potent inhibitor of intracellular glycosylation. First, we examined the effects of nonactin on syncytium formation and cytopathic activity in virus-infected baby hamster kidney cells. Nonactin effectively inhibited syncytium formation in a concentration-dependent manner, and infectious virus production was markedly reduced. However, glycoprotein synthesis was not affected. In the presence of 5 ?g/ml nonactin, we observed the intracellular accumulation of vesicular stomatitis virus-G protein as well as syncytium formation, but no significant effects on Newcastle disease virus-hemagglutinin-neuramidase glycoprotein synthesis. Our results collectively indicate that nonactin potentially inhibits glycosylation by acting as a suppressor of intracellular glycosylation trafficking.</P>
Lee, Siyoung,Kim, Eunsom,Jhun, Hyunjhung,Hong, Jaewoo,Kwak, Areum,Jo, Seunghyun,Bae, Suyoung,Lee, Jongho,Kim, Busun,Lee, Jungmin,Youn, Sulah,Kim, Somi,Kim, Miyeon,Kim, Hyunwoo,Lee, Youngmin,Choi, Dong American Society for Biochemistry and Molecular Bi 2016 The Journal of biological chemistry Vol.291 No.28
<P>Although it has been established that diabetes increases susceptibility to infections, the role of insulin (INS) in the immune response is unknown. Here, we investigated the immunological function of INS. Proinsulin dimer (pINSd) was a potent immune stimulus that induced inflammatory cytokines, but mature INS was unable to induce an immune response. An affinity-purified rabbit polyclonal antibody raised against mature IL-1α recognized IL-1α and pINS but failed to detect mature INS and IL-1β. Analysis of the pINS sequence revealed the existence of an INS/IL-1α motif in the C-peptide of pINS. Surprisingly, the INS/IL-1α motif was recognized by monoclonal antibody raised against IL-1α. Deleting the INS/IL-1α motif in pINSd and IL-1α changed their activities. To investigate the pINSd receptor, the reconstitution of IL-1 receptor 1 (IL-1R1) in Wish cells restored pINSd activity that was reversed by an IL-1R antagonist. These data suggested that pINSd needs IL-1R1 for inflammatory cytokine induction. Mouse embryo fibroblast cells of IL-1R1-deficient mice further confirmed that pINSd promotes immune responses through IL-1R1.</P>
거울을 활용한 미러링 코칭이 자유형 스트로킹 변화에 미치는 영향
이보람(Lee, Bo-Ram),윤소미(Yun, Somi),정아름(Jung, Ah-Reum),이윤빈(Lee, Yun Bin),이희진(Lee, Hee Jin),이대택(Lee, Dae Daek) 한국웰니스학회 2020 한국웰니스학회지 Vol.15 No.4
본 연구는 자유형 스트로크를 교정하기 위해 수영장 바닥 거울을 사용한 코칭이 효과적인지 분석하는데 목적이 있다, 1년 이상 수영 강습을 받은 성인 30명을 무작위로 Mirror group(MG)과 No mirror group(NMG)으로 나누어 실시하였다. MG(men=12, 32.25±8.2 yrs, 175±5.5 ㎝, 70.9±9.2 ㎏; women=3, 37.6±13.7 yrs, 160.0±3.4 ㎝, 55.6±3.5 ㎏), NMG(men=7, 28.7±6.1 yrs, 176.2±3.7 ㎝, 72.4±14.4 ㎏; women=8, 38.8±14.6 yrs, 162.1±4.4 ㎝, 54.1±7.3 ㎏)은 2.43 m x 4.57 m x 1.37 m 크기의 인공 수조에서 자유형 1분을 실시하였다. 거울제공 여부와 시간의 상호작용이 스트로크 기준점과의 차이에 미치는 효과는 1차 검사에서 NMG: 6.0±3.982 ㎝, MG: 7.67±3.266 ㎝로 집단 간 차이가 1.67 ㎝인 반면, 2차 검사결과 NMG: 5.07±3.788 ㎝, MG: 2.47±3.502 ㎝ 로 집단 간 차이가 2.6 cm 로 나타나 통계적으로 유의하였다(F=20.627, p<.001). 거울 제공 여부와 시간에 따른 스트로크 빈도에 대한 분산분석 결과, 피드백 종류와 1차, 2차 검사의 상호작용이 스트로크 빈도에 미치는 효과는 1차 결과 NMG: 57.67±6.821 score, MG: 60.73±7.294 score에서 2차 결과 NMG: 56.33±6.586 score, MG: 53.67±5.447 score로 통계적으로 유의한 차이가 있는 것으로 나타났다(F=24.807, p<.001). 거울 제공 여부와 사전사후 검사 따른 스트로크 시간에 대한 분산분석을 실시한 결과, 피드백 종류와 1차, 2차 검사의 상호작용이 스트로크 시간에 미치는 효과는 1차 NMG: 2.04±.208 sec, MG: 2.05±.250sec에서 2차 NMG: 2.15±.287 sec, MG: 2.39±.307 sec로 통계적으로 유의한 차이가 나타났다(F=10.813, p<.01). This study analyzed whether coaching recreational swimmers using pool bottom mirror for correcting front crawl stroke is effective in achieving an intended stroke. Thirty adults who enrolled swimming lessons for more than 1 year participated and were randomly divided into two groups; mirror group (MG: 12 men; 32.3±8.2 yrs, 175.0±5.5 ㎝, 70.9±9.2 ㎏: 3 women; 37.6±13.7 yrs, 160.0±3.4 ㎝, 55.6±3.5 ㎏) and control group (CG: 7 men; 28.7±6.1 yrs, 176.2±3.7 ㎝, 72.4±14.4 ㎏: 8 women; 38.8±14.6 yrs, 162.1±4.4 ㎝, 54.1±7.3 ㎏). They performed front crawl twice in a swimming flume (2.43 × 4.57 × 1.37 meter) at their chosen speed (pace of 87.9±4.2 for men, 102.7±4.7 sec/100 meter for women). Their stroke was video recorded by an underwater camera (xnb-6001, Samsung, Korea) and dry land camera (gnd-6020R, Samsung, Korea). After their first trial and recording, individual stroke was evaluated and verbal feedback was given for correction targeting full arm range stroking. During second trial, MG had a mirror (81 × 151 ㎝) installed under the bottom of flume so that they could check their stroke. CG did not use mirror during second trial. An image analyzer (Dartfish, Swiss) was used to analyze the distance between reference point (RP: where fingertip was reached over Vastus lateralis during standing till on land) and fingertip as well as stroke time (time between left hand immersion cycle). Stroke frequency was counted cycles of both arms. Two trials were compared. The distance between RP and fingertip was 7.7±3.3 and 2.5±3.5 ㎝ in MG in 1st and 2nd trial, respectively (p<0.001) while it was 6.0±4.0 and 5.1±3.8 ㎝ in CG. The stroke frequency was 60.7±7.3 in MG and 57.7±6.8 freq/min in CG at 1st trial. It was decreased to 53.7±5.4 in MG (p<0.001), but not in CG (56.3±6.6 freq/min). The stroke time was increased from 2.1±0.3 to 2.4±0.3 sec in MG (p<0.01), but it was not changed in CG (2.1±0.2 vs. 2.2±0.3 sec)(F=10.813, p<.01).
Remote Memory and Cortical Synaptic Plasticity Require Neuronal CCCTC-Binding Factor (CTCF)
Kim, Somi,Yu, Nam-Kyung,Shim, Kyu-Won,Kim, Ji-il,Kim, Hyopil,Han, Dae Hee,Choi, Ja Eun,Lee, Seung-Woo,Choi, Dong Il,Kim, Myung Won,Lee, Dong-Sung,Lee, Kyungmin,Galjart, Niels,Lee, Yong-Seok,Lee, Jae-H Society for Neuroscience 2018 The Journal of neuroscience Vol.38 No.22
<P>The molecular mechanism of long-term memory has been extensively studied in the context of the hippocampus-dependent recent memory examined within several days. However, months-old remote memory maintained in the cortex for long-term has not been investigated much at the molecular level yet. Various epigenetic mechanisms are known to be important for long-term memory, but how the 3D chromatin architecture and its regulator molecules contribute to neuronal plasticity and systems consolidation is still largely unknown. CCCTC-binding factor (CTCF) is an 11-zinc finger protein well known for its role as a genome architecture molecule. Male conditional knock-out mice in which CTCF is lost in excitatory neurons during adulthood showed normal recent memory in the contextual fear conditioning and spatial water maze tasks. However, they showed remarkable impairments in remote memory in both tasks. Underlying the remote memory-specific phenotypes, we observed that female CTCF conditional knock-out mice exhibit disrupted cortical LTP, but not hippocampal LTP. Similarly, we observed that CTCF deletion in inhibitory neurons caused partial impairment of remote memory. Through RNA sequencing, we observed that CTCF knockdown in cortical neuron culture caused altered expression of genes that are highly involved in cell adhesion, synaptic plasticity, and memory. These results suggest that remote memory storage in the cortex requires CTCF-mediated gene regulation in neurons, whereas recent memory formation in the hippocampus does not.</P>
Trafficking Inhibition of Bruceanol B as a Radical-Producing Antibiotic
Lee, Do-Seung,Boo, Kyung-Hwan,Woo, Jin-Kyu,Hong, Quan-Chun,Cho, Somi K.,Park, Se-Pill,Lee, Dong-Sun,Riu, Key Zung The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
Bruceanol B, isolated from a soil microbe strain DS4, generated oxygen radicals in Bacillus subtilis lysates. Bruceanol B inhibited the trafficking of viral glycoprotein in virus-infected baby hamster kidney (BHK) cells. Bruceanol B also effectively inhibited syncytium formation in a dose-dependent manner. However, glycoprotein synthesis was not affected by the compound. Results indicate that bruceanol B generating oxygen radical has inhibitory activity in the trafficking of viral glycoprotein of BHK cell.