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      • SCOPUSKCI등재

        Biofilter 를 이용한 VOC 처리 특성 및 미생물 첨가에 의한 영향

        김종화,김소영,박대원,김우식,김응빈,김승수,함승주 한국화학공학회 2001 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.39 No.3

        본 실험에서는 실험실크기 biofilter에서 온도, bed length, 체류시간(가스흐름량), 농도, 적정 수분량 지역 등을 대상으로 toluene분해 실험을 진행하였다. 본 실험에서의 온도의존성 결과로서, 저온보다 고온에서 효율이 더 상승하는 것으로 나타났으며, 최적의 효율을 나타내는 온도는 모든 농도에서 32-35℃임을 알 수 있었다. 가스흐름량 대비 제거효율은 도입되는 가스흐름량이 많아짐에 따라 감소하였으며, 가스흐름량에 따른 제거속도와 제거효율은 서로 상반되는 경향을 나타냈다. 도입 농도 251 g/㎥ 이하에서는 0차 반응의 결과를 나타내었으며, 같은 체류시간에서 bed length가 길어짐에 따라 반응속도는 증가하였지만 지속적으로 증가하지는 않았다. 초기의 미생물 서식양상과 처리후의 서식양상을 비교하면 agglomeration으로 인해 투과 기공이 작아지면서 셀 자체의 증식활동이 저해됨으로써 제거효율이 저하된다는 것을 알 수 있었다. 그 외에 최적 수분 지역 실험을 통하여 bed length에 따른 반응속도를 고찰하였으며 cell의 CFU(Colony Forming Unit)측정을 통해 최적수분지역이 cell의 증식활동에 미치는 영향을 고찰하였다. In this study, the degradation of toluene by lab-scale biofilter inoculated with pseudomonas putida DK-1 was investigated with variation of the several environmental parameters, such as temperature, bed length, residence time (gas flow rate), concentration and optimal humidity zone. Removal efficiency increased as the operating temperature increased and the optimal temperature range to treat toluene gas was found to be 32-35℃. The effect of gas flow rate on toluene degradation was investigated at different gas flow rates of 20-100 ㎖/min. Increasing the gas flow rate showed an inverse effect on the elimination rate (ER) and the removal efficiency (RE). The removal efficiency was roughly constant and consequently the biodegradation rate was independent of concentration of toluene at the inlet load lower than 251 g/㎥. The optimal humidity dominance zone (40-60%) increased as bed length increased. The biodegradation rate of the toluene with respect to the bed length (3, 6, 9, 12 and 15 ㎝) increased up to 80 h but was then independent of the bed length after 80 h except for the 3 ㎝ bed length.

      • SCISCIESCOPUS

        The functional relationship between co-repressor N-CoR and SMRT in mediating transcriptional repression by thyroid hormone receptor alpha.

        Choi, Kyung-Chul,Oh, So-Young,Kang, Hee-Bum,Lee, Yoo-Hyun,Haam, Seungjoo,Kim, Ha-Il,Kim, Kunhong,Ahn, Young-Ho,Kim, Kyung-Sup,Yoon, Ho-Geun Biochemical Society 2008 Biochemical journal Vol.411 No.1

        <P>A central issue in mediating repression by nuclear hormone receptors is the distinct or redundant function between co-repressors N-CoR (nuclear receptor co-repressor) and SMRT (silencing mediator of retinoid and thyroid hormone receptor). To address the functional relationship between SMRT and N-CoR in TR (thyroid hormone receptor)-mediated repression, we have identified multiple TR target genes, including BCL3 (B-cell lymphoma 3-encoded protein), Spot14 (thyroid hormone-inducible hepatic protein), FAS (fatty acid synthase), and ADRB2 (beta-adrenergic receptor 2). We demonstrated that siRNA (small interfering RNA) treatment against either N-CoR or SMRT is sufficient for the de-repression of multiple TR target genes. By the combination of sequence mining and physical association as determined by ChIP (chromatin immunoprecipitation) assays, we mapped the putative TREs (thyroid hormone response elements) in BCL3, Spot14, FAS and ADRB2 genes. Our data clearly show that SMRT and N-CoR are independently recruited to various TR target genes. We also present evidence that overexpression of N-CoR can restore repression of endogenous genes after knocking down SMRT. Finally, unliganded, co-repressor-free TR is defective in repression and interacts with a co-activator, p300. Collectively, these results suggest that both SMRT and N-CoR are limited in cells and that knocking down either of them results in co-repressor-free TR and consequently de-repression of TR target genes.</P>

      • Biofilter 를 이용한 VOC 처리특성 및 미생물 첨가에 의한 영향 연구

        김종화,김소영,김우식,김응빈,김승수,김빈,함승주 한국공업화학회 1999 응용화학 Vol.3 No.1

        The removal effects of VOC vapors in a pilot-scale biofilter have been studied. Biofiltration test was performed in a biofilter fed downward with contaminated air at ambient conditions. The biofilter was packed with the bark of a tree(1∼2 ㎝ size). The biofilter was operated under various inlet-airstream benzene concentrations. The flow-rate and temperature in the biofilter were 0.398 L/min., 5∼15℃, respectively. We investigated the removal efficiency variation of benzene with different concentrations were changed. Also, we studied the effects of VOC treatment when microorganisms (Pseudomonas) was added to the biofilter. The results showed that the removal efficiency was fairly improved by addition of microorganisms.

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