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      • KCI등재

        Development of a selective media for detecting Campylobacter spp. in chicken carcasses using avibactam supplemented mCCDA

        김빈,서건호 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.8

        Campylobacter spp. are major causes of gastrointestinalinfections worldwide, and are commonlyidentified using modified-charcoal-cefoperazone-deoxycholateagar (mCCDA). However, the efficacy of thisscreening technique is often hindered by overgrowth ofcompeting flora, such as extended-spectrum b-lactamase(ESBL)-producing Escherichia coli. Thus, in the presentstudy we supplemented mCCDA with a recently developedESBL inhibitor, avibactam (A-mCCDA). We inoculatedmCCDA and A-mCCDA plates with 25 strains each ofCampylobacter spp. and ESBL-producing E. coli, andthereby determined that the optimum avibactam concentrationrequired to inhibit ESBL-producing E. coli was0.0625 mg/L. At this concentration, a significantly higherproportion of Campylobacter spp. was isolated usingA-mCCDA compared to that using mCCDA (P\0.05). Thus, the results of the present study support the use ofA-mCCDA to improve current Campylobacter screeningmethods.

      • KCI등재

        분산 모형에 따른 습지경관의 생태 네트워크 특성 분석

        김빈,박제량 한국습지학회 2019 한국습지학회지 Vol.21 No.1

        동식물의 서식지 제공 및 수문학적 조절 등 다양한 생태계 서비스를 제공하는 습지는 경관 내 습지간의 상호작용을 통해생태계를 구성한다. 따라서 습지경관에서의 생태계의 리질리언스 등의 기능을 평가하기 위해서는 습지의 동역학적 특성과더불어 습지간에 형성되는 생태적 연결성 분석이 필요하다. 본 연구에서는 습지를 생태학적 노드로 정의하여 습지 서식종의분산 모형에 따라 발생하는 습지간 연결을 통해 생태 네트워크를 생성하고 네트워크 분석 방법을 통해 생태 네트워크의특성을 비교, 분석하였다. 임계거리를 활용한 분산모델 (threshold distance)의 경우 확률적인 분산모델(exponential dispersal kernel, heavy-tailed dispersal model)과 비교하여 국지적인 군집화가 높으나 습지 간의 이동에는 비효율적인 것으로 나타났다. 반면 확률적 분산모델의 경우 국지적인 군집화는 낮게 나타나나 습지 경관 내에서의 이동은 효율적인 것으로 나타남에 따라분산 모델에 따른 생태 네트워크 특성에 차이가 발생함을 확인하였다. 본 연구는 향후 습지경관 리질리언스 분석을 위한생태 네트워크 구축의 기초자료로 활용될 수 있을 것으로 사료된다. Wetlands that provide diverse ecosystem services, such as habitat provision and hydrological control of flora and fauna, constitute ecosystems through interaction between wetlands existing in a wetlandscape. Therefore, to evaluate the wetland functions such as resilience, it is necessary to analyze the ecological connectivity that is formed between wetlands which also show hydrologically dynamic behaviors. In this study, by defining wetlands as ecological nodes, we generated ecological networks through the connection of wetlands according to the dispersal model of wetland species. The characteristics of these networks were then analyzed using various network metrics. In the case of the dispersal based on a threshold distance, while a high local clustering is observed compared to the exponential dispersal kernel and heavy-tailed dispersal model, it showed a low efficiency in the movement between wetlands. On the other hand, in the case of the stochastic dispersion model, a low local clustering with high efficiency in the movement was observed. Our results confirmed that the ecological network characteristics are completely different depending on which dispersal model is chosen, and one should be careful on selecting the appropriate model for identifying network properties which highly affect the interpretation of network structure and function.

      • KCI등재

        관광경험과 기억저장이 관광자 만족과 충성도에 미치는 영향에 관한 연구

        김빈,가정혜 관광경영학회 2019 관광경영연구 Vol.90 No.-

        The cost of creating new customers is five times higher than that of maintaining old ones, so strategically maintaining the ones already captivated is far more meaningful than creating new ones. When travelers are satisfied with their trips, the possibility of revisiting is higher. But not all satisfied travelers revisit the destination or recommend it to others. Tourism are regarded as a behavior that consumers pay for pleasant experiences and ultimately acquire pleasant memories. Therefore, the most important thing for tourism behavior is to get relevant memories of valuable travel experiences. The purpose of this study is to explore the relationship between memory storage which is influenced by travel experience and tourists' satisfaction and loyalty. The results of this study are as follow: Firstly, travel experience has an impact on memory storage, in which life vitality, knowledge and sensory experience has an impact on both in short-term memory and long-term memory. Secondly, short-term memory has an impact on satisfaction while long-term memory does not have an impact on tourists' satisfaction. Thirdly, long-term memory has a great impact on loyalty. Lastly, tourist satisfaction has a positive impact on loyalty.

      • KCI등재

        국내 모돈에 대한 발굽 병변 조사

        김 빈,정종화,정현규,한정희 한국동물위생학회 2019 韓國家畜衛生學會誌 Vol.42 No.4

        Claw lesion is one of the major causes of lameness in sows and the lameness is one of the reasons for culling of sows from the swine herd. This survey was conducted on sows in total of 8 farms with a total of 684 pigs. The lesions observed in the study were horizontal and vertical claw cracks (CWH & CWV), differences in the length of toes (T) and/or in the length of dew claws (DC) according to crates, breeds and parity of sows. Scores of claw lesions were assigned on 0, 1, 2, and 3 at each foot depend-ing on the severity of the claw lesions. The sows’ parity was classified into 1 to 2, 3 to 4, 5 to 8. Prevalence rate of claw lesions in sows raised on punched plastic crate was higher than that in sows raised on tribar crate. In prevlence rate by breeds, inbreed Landrace sows showed higher than that of Yorkshire sows. According to 4 claw lesions, the length of DC was the highest at parity 3∼4 and CWV was the lowest at parity 3∼4 in F1 crossbred sows raised on punched plastic crates, respectively. In Yorkshire breeds raised on punched plastic crates, the length of DC at parity 5∼8 was the lowest and CWH was the highest at parity 3∼4, respectively. In Landrace breeds raised on tribar crates, CWH was the highest at parity 1∼2 and CWV was the lowest at parity 3∼4, respectively. The high parity in F1 crossbred sows showed high prevalence rate of claw lesions than that of low parity and other inbreed sows, respectively. These results may be useful as elementary data in establishment of welfare quality protocol and preventive measures to reduce economic losses from craw lesions in sows.

      • KCI등재

        토지이용변화에 따른 거시적 습지경관에서의 생태네트워크의 구조 및 기능적 변화 평가

        김빈(Bin Kim),박제량(Jeryang Park) 응용생태공학회 2020 Ecology and resilient infrastructure Vol.7 No.3

        다양한 생태계 서비스를 제공할 수 있는 습지는 지속가능한 수질 개선 및 기후변화로 인한 영향의 완충작용 등 중요한 자연기반해법기술로 간주되어 왔다. 특히 토지이용 변화, 기후 변화 및 수문 변화에 따른 영향 저감을 위한 습지 보전의 중요성은 부각되었으나 경관규모에서의 거시적 자연기반해법기술 가능성의 검토가 미비하였다. 이에 본 연구에서는 생태 네트워크 분석을 통한 공학적 솔루션 제공 가능성을 검토하기 위해 습지경관 가상 서식종의 이동모델을 기반으로 형성된 습지 생태네트워크를 이용하여 토지이용변화에 따른 생태네트워크의 구조적, 기능적 특성 (연결성, 이동 효율성 및 집단화 계수)이 어떻게 변화하는지 분석하였다. 이를 위해 습지 밀도가 다른 네 구역의 토지이용변화를 가정하여 두 가지의 초기 면적조건에 대한 각 구역의 동시다발적 토지이용변화를 통해 생태 네트워크 특성의 변화를 분석하였다. 모든 분석결과에서 습지밀도가 높은 구역이 파괴된 경우 생태네트워크의 평균 연결성과 이동 효율성이 크게 감소하였으며, 특히 허브 (매우 높은 연결성을 지니는 노드)가 포함된 구역의 습지가 제거될 때 급격한 감소가 발생하는 것을 확인하였다. 반면, 집단화 계수는 증가하는 것으로 관찰되었다. 이를 통해 토지이용변화에 따른 생태네트워크에 대한 영향을 평가할 수 있으며 특히 향후 매개중심성 분석을 추가하여 적합한 대체습지를 조성할 수 있는 자연기반의 공학적 솔루션을 제공할 수 있을 것으로 사료된다. Wetlands, which provide various ecological services, have been regarded as an important nature-based solution for, for example, sustainable water quality improvement and buffering of impacts from climate change. Although the importance of conserving wetlands to reduce the impacts of various perturbations (e.g., changes of land use, climate, and hydrology) has been acknowledged, the possibility of applying these efforts as a nature-based solution in a macro-scale (e.g., landscape) has been insufficient. In this study, we examine the possibility of ecological network analysis that provides an engineering solution as a nature-based solution. Specifically, we analyzed how land use change affects the structural and functional characteristics (connectivity, network efficiency, and clustering coefficient) of the ecological networks by using the ecological networks generated by multiple dispersal models of the hypothetical inhabiting species in wetlandscape. Changes in ecological network characteristics were analyzed through simultaneously removing wetlands, with two initial conditions for surface area, in the zones where land use change occurs. We set a total number of four zones of land use change with different wetland densities. All analyses showed that mean degree and network efficiency were significantly reduced when wetlands in the zones with high wetland density were removed, and this phenomenon was intensified especially when zones contained hubs (nodes with high degree). On the other hand, we observed the clustering coefficient to increase. We suggest our approach for assessing the impacts of land use change on ecological networks, and with additional analysis on betweenness centrality, we expect it can provide a nature-based engineering solution for creating alternative wetlands.

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