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Micro-groove patterned PCL patches with DOPA for rat Achilles tendon regeneration
Sihyeon Park,이민석,Jin Jeon,Juhan Lim,조현철,Suk Ho Bhang,Hee Seok Yang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.105 No.-
Achilles tendon regeneration using current tissue engineering techniques requires morphometricallymimetic tendon biomaterials to attain the required microstructural tendon tissue and to achieve the necessarymechanical properties. In this study, we used nano- (400 nm) and micro-groove (5000 nm) patternedpolycaprolactone patches (PCL-400, PCL-5000), which offer adequate elastic modulus andbiocompatibility. The PCL patches were fabricated via capillary force lithography and were surface modifiedwith 3,4-dihydroxy-L-phenylalanine (DOPA) for increased hydrophilicity. We hypothesized that theresulting biologically surface-modified elastic groove patterned patches would enhance the cell behaviorsin vitro and the rat Achilles tendon regeneration in vivo. The micro-groove patterned PCL patcheswith DOPA coating (DOPA-PCL-5000) exhibited excellent cell elongation, tenogenic differentiation andYAP expression of the mesenchymal stem cells in our in vitro study and these results were further confirmedby application to the rat Achilles tendon rupture model in our in vivo study. DOPA-PCL-5000induced dramatic regeneration of rat Achilles tendon compared to the other groups, which was furtherconfirmed by the Achilles functional index and histological analysis at 8 weeks. In conclusion, DOPAPCL-5000 can be used not only for damaged Achilles tendon, but also for various functional tendon regenerationtechniques such as rotator cuff, patellar tendon, and flexor tendon.
Sihyeon Kim,Se Jin Lee,Mi Rong Lee,Jong Cheol Kim,So Eun Park,Jae Su Kim 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.04
Beauveria bassiana (Bb) is a potential biopesticide against western flower thrips. To understand molecular characteristics of two Bb isolates (ERL836 and JEF-007), whole genome sequencing and RNA-seq were performed. The whole genome of Bb ERL836 was sequenced (3,934,801,443 bp) using Pacbio sequencing and 15 contigs were analyzed. To understand the molecular mechanisms of pathogenicity in two Bb genome (before infection and after infection), next generation sequencing (NGS) was analyzed to compare transcriptomes. A result of differentially expressed gene (DEG) showed that 2,689 contigs were up-regulated and 2,619 contigs were down-regulated. Randomly selected five genes were validated to be highly up-regulated. This work can provide an understanding of the interaction between Bb and westetn flower thrips.
Establishment of entomopathogenic fungal virulence assay against Protaetia brevitarsis seulensis
Sihyeon Kim,Seok Ju Lee,So Eun Park,Jong Cheol Kim,Mi Rong Lee,Se Jin Lee,Jae Su Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.04
Many turfgrass pests, Ectinohoplia rufipes, Exomala orientalis and Popillia quadriguttata, cause serious damage to grass fields in Korea. In this study, control of turfgrass pests using entomopathogenic fungi were investigated using Protaetia brevitarsis seulensis as an alternative insect. In a preliminary experiment, some Metathizium sp. showed ca. 60% virulence after 7 days exposure to the entomopathogenic fungi. Compared to the contact-exposure, overall a spray treatment showed lower control efficacy against P. brevitarsis seulensis. Furthermore, mortality of various developmental stages of P. brevitarsis seulensis was investigated under laboratory conditions. This work suggests that when treated with fungi mortality of P. brevitarsis seulensis was closely related with developmental stage. These results provide a basic information for the control of P. brevitarsis seulensis using entomopathogenic fungi
Sihyeon Kim,Jong Cheol Kim,Mi Rong Lee,So Eun Park,Dongwei Li,Sehyeon Baek,Laila Gasmi,Min Sung Jo,Tae Young Shin,Junheon Kim,Jae Su Kim 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04
Insect killing fungus Beauveria bassiana has been widely studied as a biological control agent. However, many studies have been focused on lab or field-based management. Herein this work, comparison of three B. bassiana strains was investigated under a molecular level. The whole genome sequences of ERL836, JEF-007 were analyzed by PacBio (35.5 Mb of ERL836 and 36.5 Mb of JEF-007) and ARSEF2860 referenced from GenBank (33.7 Mb). To compare the three strains, virulence, thermotolerance and chemical resistance were assayed. The transcriptomes of non-infecting B. bassiana and infecting B. bassiana against western flower thrips were analyzed using RNA-seq. This work can provide that genome features, functions, morphology and gene expression could be different under the molecular level, even if in the same species.
( Sihyeon Kim ),( Jong Cheol Kim ),( Se Jin Lee ),( Mi Rong Lee ),( So Eun Park ),( Dongwei Li ),( Sehyeon Baek ),( Tae Young Shin ),( Laila Gasmi ),( Jae Su Kim ) 한국균학회 2020 Mycobiology Vol.48 No.2
Root-feeding Scarabaeidae, particularly white grubs are considered among the most harmful coleopteran insect pests in turfgrass. In this work, sixteen entomopathogenic fungal species were assayed against flower chafer beetle, Protaetia brevitarsis (Coleoptera: Scarabaeidae) and Metarhizium anisopliae JEF-314 showed high virulence. The control ability of the isolate JEF-314 has been in detail tested for a model insect flower chafer beetle. Further analyses showed insect stage-dependent virulence where the fungal virulence was the highest against smaller instar larvae. Additionally, we confirmed that millet-based solid cultured granule was effective against the soil-dwelling larval stage. The isolate also showed a similar ability for a representative pest (Popillia spp.) in laboratory conditions. Our results clearly suggest a high potential of M. anisopliae JEF-314 to control the flower chafer beetle, possibly resulting in controlling of root-feeding white grubs in turfgrass. Based on the insect life cycle and susceptibility to the fungus, late spring and summer time would be the optimum time to apply JEF-314 granules for an effective control. Further characterization of the efficacy of the fungus under field conditions against the Scarabaeidae beetles might provide an efficient tool to control this beetle in an environment-friendly way.