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Kinetics of Enriched Chitinase as Extracellular Metabolite in Beauveria bassiana
Subhoshmita Mondal,Siddhartha Datta,Alakananda Mukherjee,Pinaki Bhattacharya 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.1
Beauveria bassiana, one of the most common entomopathogenic fungi, has been isolated, pre defined and characterized in-house from soil of tea cultivation area. Experiments have been performed to verify the presence of chitinase as intracellular metabolite and its release as extracellular product rendering the spores with biopesticide activity. Although there are many responsible enzymes for the pest killer action of B. bassiana, binding property of chitinase depending on presence as well as absence of serine supplemented in the media has been studied with respect to the production and kinetics. A programmed investigation conclusively indicates that the isolated spore (hyphae) of B. bassiana has been metabolically enriched with the enzyme chitinase in presence of an externally added amino acid serine with its inhibitory kinetics.
Kinetics of Enriched Chitinase as Extracellular Metabolite in Beauveria bassiana
Mondal, Subhoshmita,Datta, Siddhartha,Mukherjee, Alakananda,Bhattacharya, Pinaki The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.1
Beauveria bassiana, one of the most common entomopathogenic fungi, has been isolated, pre defined and characterized in-house from soil of tea cultivation area. Experiments have been performed to verify the presence of chitinase as intracellular metabolite and its release as extracellular product rendering the spores with biopesticide activity. Although there are many responsible enzymes for the pest killer action of B. bassiana, binding property of chitinase depending on presence as well as absence of serine supplemented in the media has been studied with respect to the production and kinetics. A programmed investigation conclusively indicates that the isolated spore (hyphae) of B. bassiana has been metabolically enriched with the enzyme chitinase in presence of an externally added amino acid serine with its inhibitory kinetics.
Performance of an OFF-Board Test Rig for an Automotive Air Conditioning System
SANTANU P. DATTA,PRASANTA K. DAS,SIDDHARTHA MUKHOPADHYAY 대한설비공학회 2013 International Journal Of Air-Conditioning and Refr Vol.21 No.3
An o®-board test bench has been developed for evaluating the performance of an automotive air conditioning system. The facility consists of the mechanical hardware used in an automobile along with a large number of additional sensors and a standalone control system that mimics the operations in a car. The performance of the individual components and the system has been investigated for two major independent variables of running automobiles, namely the compressor speed and the speed of the evaporator fan. Correlations for the volumetric e±ciency of the compressor and COP of the system have been developed. While the refrigerant °ow rate is in°uenced mainly by compressor speed, the COP depends on both the operating variables. The investigation also establishes that the design of the rig, the selection and placement of the sensors and the method of measurement are suitable for its further deployment under dynamic condition and for fault diagnosis.
Meghana Prabhu,Deepali Jain,Siddhartha Datta Gupta,Chandrasekhar Bal,Rakesh Kumar 대한핵의학회 2018 핵의학 분자영상 Vol.52 No.5
Thyroid carcinoma is the most common neoplasm of endocrine malignancies. Differentiated thyroid carcinoma (DTC) constitutes90% of the thyroid carcinomas, rest being medullary thyroid carcinoma (MTC), and anaplastic thyroid carcinoma (ATC). Distant metastases occur in up to 10% of patients with DTC. Metastases to axillary lymph nodes (ALN) are very rare. As perliterature, only 25 cases have been reported. We report an unusual case of 47-year-old male with Hürthle cell carcinoma of thethyroid presenting with a solitary axillary lymph node metastasis 17 years after thyroidectomy, along with review of literature.
Papita Das Saha,Shamik Chowdhury,Siddhartha Datta,Shyamal K Sanyal 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.8
The feasibility of applying natural, untreated clayey soil as low-cost alternative adsorbent for Pb(II) removal from aqueous solutions was investigated with a batch experimental set-up. Experiments were carried out as a function of initial solution pH (1-8), contact time (10-360 min), initial Pb(II) concentration (20-100 mg L−1), adsorbent dose (0.5-5 g) and temperature (303-333 K). Adsorption equilibrium data were well described by the Langmuir isotherm with maximum adsorption capacity of 121.86 mg g−1 at 303 K. Adsorption of Pb(II) followed pseudo-second-order kinetics. Gibbs free energy (ΔG0) was spontaneous for all interactions, and the adsorption process exhibited exothermic enthalpy values. The adsorbent was easily regenerated by using 0.1M HNO3 solution and was reused for five sorptiondesorption cycles without any considerable loss in adsorption capacity. It could be concluded that clayey soil may be used as an inexpensive and effective adsorbent without any treatment or any other modification for the removal of Pb(II)ions from aqueous solutions.
Prasenjit Das,Ritika Rampal,Sonakshi Udinia,Tarun Kumar,Sucharita Pilli,Nahid Wari,Imtiaz Khan Ahmed,Saurabh Kedia,Siddhartha Datta Gupta,Dhiraj Kumar,Vineet Ahuja 대한장연구학회 2018 Intestinal Research Vol.16 No.3
Background/Aims: Classical M1 macrophage activation exhibits an inflammatory phenotype while alternative M2 macrophage activation exhibits an anti-inflammatory phenotype. We aimed to determine whether there are discriminant patterns of macrophage polarization in Crohn’s disease (CD) and intestinal tuberculosis (iTB). Methods: Colonic mucosal biopsies from 29 patients with iTB, 50 with CD, and 19 controls were examined. Dual colored immunohistochemistry was performed for iNOS/CD68 (an M1ϕ marker) and CD163/CD68 (an M2ϕ marker), and the ratio of M1ϕ to M2ϕ was assessed. To establish the innate nature of macrophage polarization, we analyzed the extent of mitochondrial depolarization, a key marker of inflammatory responses, in monocyte-derived macrophages obtained from CD and iTB patients, following interferon-γ treatment. Results: M1ϕ polarization was more prominent in CD biopsies (P=0.002) than in iTB (P=0.2) and control biopsies. In granuloma-positive biopsies, including those in CD, M1ϕ predominance was significant (P=0.001). In iTB, the densities of M1ϕ did not differ between granuloma-positive and granuloma-negative biopsies (P=0.1). Interestingly, higher M1ϕ polarization in CD biopsies correlated with high inflammatory response exhibited by peripheral blood-derived monocytes from these patients. Conclusions: Proinflammatory M1ϕ polarization was more common in colonic mucosa of CD patients, especially in the presence of mucosal granulomas. Further characterization of the innate immune system could help in clarifying the pathology of iTB and CD.
Shouriyo Ghosh,Brijnandan Gupta,Pavan Verma,Sreenivas Vishnubathla,Sujoy Pal,Nihar R Dash,Siddhartha Datta Gupta,Prasenjit Das 대한장연구학회 2018 Intestinal Research Vol.16 No.1
Background/Aims: Aberrant crypt foci (ACF) are early microscopic lesions of the colonic mucosa, which can be detected bymagnified chromoendoscopy. Herein, we have investigated whether ACF identified in different clinical groups can be differentiated based on their characteristics. Methods: Macroscopically unremarkable mucosal flaps were collected from 270 freshcolectomies and divided into 3 clinical groups: colorectal carcinoma (group A), disease controls having known pre-neoplasticpotential (group Bc), and disease controls without risk of carcinoma development (group Bn). Topographic and histologic anal-ysis, immunohistochemistry, and molecular studies (high-resolution melt curve analysis, real-time polymerase chain reaction,and Sanger sequencing) were conducted for certain neoplasia-associated markers. Results: ACF were seen in 107 cases, out ofwhich 72 were left colonic ACF and 35 right colonic ACF (67.2% vs. 32.7%, P =0.02). The overall density of left colonic ACF was0.97/cm, which was greater than the right colonic ACF density of 0.81/cm. Hypercrinia was present in 41 out of 72 left colonicACF and in 14 out of 35 right colonic ACF (P =0.01). Immunohistochemical expression of p53 was also greater in left colonicACF than in right colonic ACF (60.5% vs. 38.2%, P =0.03). However, ACF identified among the 3 clinical groups did not show anydistinguishing topographic, histological, or genetic changes. Conclusions: Left colonic ACF appear to be high-risk based ontheir morphological and prototypic tumor marker signature. ACF identified in different clinical groups do not show significantgenotypic or topographic differences. Further detailed genetic studies are required to elucidate them further.