LncRNA DLG1-AS1 Promotes Cancer Cell Proliferation in Triple Negative Breast Cancer by Downregulating miR-203

Shuyan Li 한국유방암학회 2020 Journal of breast cancer Vol.23 No.4

Purpose: The oncogenic role of long non-coding RNA (lncRNA) DLG1-AS1 has been studied in cervical cancer, but its involvement in triple negative breast cancer (TNBC) is unknown. Here, we aimed to investigate the possible role and underlying mechanism of DLG1-AS1 in TNBC. Methods: The differential expression of DLG1-AS1 and miR-203 in TNBC tissues and cells was determined using quantitative polymerase chain reaction assays. Correlations between DLG1-AS1 and miR-203 expression across TNBC tissues and non-tumor tissues were analyzed using Spearman rank correlation test. The effects of DLG1-AS1 and miR-203 overexpression, and DLG1-AS1 knockdown on the metastasis of BT-549 and MDA-MB-157 cells were evaluated using a transwell assay. The effects of DLG1-AS1 and miR-203 overexpression on the proliferation of BT-549 and MDA-MB-157 cells were evaluated using Cell Counting Kit-8 and cell colony formation assays. Results: We found that DLG1-AS1 was upregulated whereas miR-203 was downregulated in tumor tissues of patients and in TNBC cells compared to the adjacent healthy tissues of patients with TNBC and in normal breast MCF-10A cells, respectively. Further, DLG1-AS1 and miR-203 were inversely correlated in tumor tissues. DLG1-AS1 overexpression mediated downregulation of miR-203, whereas miR-203 overexpression had no significant effects on DLG1-AS1 expression. DLG1-AS1 expression was increased, whereas miR-203 levels were decreased with advancing clinical stages. TNBC cell migration was promoted by DLG1-AS1 overexpression and inhibited by miR-203 overexpression or DLG1-AS1 knockdown. Moreover, TNBC cell proliferation was promoted by DLG1-AS1 overexpression and inhibited by miR-203 overexpression. Further, miR-203 overexpression reduced the effects of DLG1-AS1 overexpression. Conclusion: These results indicate that DLG1-AS1 may promote cancer cell proliferation in TNBC by downregulating the tumor suppressor miR-203.

Power Saving and Improving the Throughput of Spectrum Sharing in Wideband Cognitive Radio Networks

Shiyin Li,Shuyan Xiao,Maomao Zhang,Xiaoguang Zhang 한국통신학회 2015 Journal of communications and networks Vol.17 No.4

This paper considers a wideband cognitive radio network which can simultaneously sense multiple narrowband channels and thus aggregate the detected available channels for transmission and proposes a novel cognitive radio system that exhibits improved sensing throughput and can save power consumption of secondary user (SU) compared to the conventional cognitive radio system studied so far. More specifically, under the proposed cognitive radio system, we study the problem of designing the optimal sensing time and power allocation strategy, in order to maximize the ergodic throughput of the proposed cognitive radio system under two different schemes, namely the wideband sensing-based spectrum sharing scheme and the wideband opportunistic spectrum access scheme. In our analysis, besides the average interference power constraint at primary user, the average transmit power constraint of SU is also considered for the two schemes and then a subgradient algorithm is developed to obtain the optimal sensing time and the corresponding power allocation strategy. Finally, numerical simulations are presented to verify the performance of the two proposed schemes.

Constructing the Core User Set for Collaborative Recommendation Based on Samples Selection Idea

Zhang Li,Yu Lei,Cao Shuyan 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.3

As an effective recommendation technology to solve "information overload" problem, collaborative filtering has widly attracted attention of scholars from various fields. First, this paper proposes a method for measuring the recommending ability of user based on popularity and long-tailed distribution. Then, a global core user set is constructed for recommadition based on the recommending ability and samples selection idea in data mining, aimming to take advantage of users in the different part of the long tail distribution and reduce computing complexity of the algorithm without lowing the recommended performance. Experimental results show that the algorithm is effective and can be used to solve the real-time problem and cold start recommendation.

Ultrastructure structure of antennal sensilla of carabid beetle Carabus elysii Thomson, 1856 (Coleoptera: Carabidae)

Wen‐Bo Li,Naiyi Liu,Qin XU,Min Sun,Jie Fang,Shuyan Wang 한국곤충학회 2019 Entomological Research Vol.49 No.2

The sensilla type, number and distribution in male and female adults of Carabus elysii Thomson, 1856 (Coleoptera: Carabidae) were studied using scanning electron microscopy. The results showed that there are seven categories and 12 types of sensilla in C. elysii adults: three types of S.Ch, three types of S.T, two types of S.B and one each of B.B, S.Co, S.Ca and S.Cam. There is no difference between male and female in the types of sensilla. Apart from the significant difference in the number and distribution of S.B.2, S.Co and S.Ca between male dorsal and ventral surfaces, there are no significant differences between male and female antennae. In general, the number of sensilla in females is larger than that in males. The results provide a basic reference for future ultrastructure, electrophysiological, and comparative behavioral studies of Carabus species.

The complete mitochondrial genome of Damora sagana and phylogenetic analyses of the family Nymphalidae

Naiyi Liu,Na Li,Pengyu Yang,Chunqin Sun,Jie Fang,Shuyan Wang 한국유전학회 2018 Genes & Genomics Vol.40 No.1

The monotypic genus Damora (Nymphalidae, Heliconiinae) contains a single species, Damora sagana, which is widely distributed across southern China. Herein, its complete mitogenome was sequenced to further understand lepidopteran mitogenome characteristics, reconstruct the nymphalid family phylogeny, and infer the subdivision of Heliconiinae species. The circular mitogenome was 15,151 bp long, abundant in A and T, and comprised of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and one control region with a gene arrangement typical of lepidopteran mitogenomes. ATN codons initiated all PCGs, except cytochrome c oxidase subunit 1 (COX1), which was initiated by a CGA sequence as has been observed in other lepidopterans. Three PCGs (COX1, COX2 and ND4) employed a single T termination signal, whereas others had the typical complete termination codon (TAA). All tRNA genes were folded into the typical cloverleaf structure except for tRNA-Ser (AGN). The A+T-rich region included the conserved motif ‘ATAGA’ followed by a 17 bp poly-T stretch, which was also observed in tribe Argynnini mitogenomes. A phylogenetic tree was constructed via multiple methods using the 13 PCGs data of D. sagana and other available mitogenomes of nymphalid species. All three phylogenetic trees yielded the same topology. These results were consistent with those from previous studies of most major nymphalid groups, except those regarding tribe subdivision in certain subfamilies such as Argynnini + (Acraeini + Heliconiini) for Heliconiine. Furthermore, our analyses identified that the genus Cethosia was grouped with the genus Acraea composing the tribe Acraeini with strong support.

Mathematical Analysis Power Spectrum of M-ary MSK and Detection with Optimum Maximum Likelihood

( Zheng Niu ),( Yuzhong Jiang ),( Shuyan G Jia ),( Zhi Huang ),( Wenliang Zou ),( Gang Liu ),( Yu Li ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.8

In this paper, the power spectral density(PSD) for Multilevel Minimum Shift Keyed signal with modulation index h=1/2 (M-ary MSK) are derived using the mathematical method of the Markov Chain model. At first, according to an essential requirement of the phase continuity characteristics of MSK signals, a complete model of the whole process of signal generation is built. Then, the derivations for autocorrelation functions are carried out precisely. After that, we verified the correctness and accuracy of the theoretical derivation by comparing the derived results with numerical simulations using MATLAB. We also divided the spectrum into four components according to the derivation. By analyzing these figures in the graphic, each component determines the characteristics of the spectrum. It is vital for enhanced spectral characteristics. To more visually represent the energy concentration of the main flap and the roll-down speed of the side flap, the specific out-of-band power of M-ary MSK is given. OMLCD(Optimum Maximum Likelihood Coherent Detection) of M-ary MSK is adopted to compare the signal received with prepared in advance in a code element T to go for the best. And M-ary MSK BER(Bit Error Rate) is compared with the same ary PSK (Phase Shift Keying) with M=2,4,6,8. The results show the detection method could improve performance by increasing the length of L(memory inherent) in the phase continuity.

Analytical Models for the Electric Potential, Threshold Voltage and Drain Current of Long-channel Junctionless Double-gate Transistors

Zhihao Ding,Guang-Xi Hu,Ran Liu,Ling-Li Wang,Shuyan Hu,Xing Zhou 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.8

Analytical models for the electric potential, threshold voltage and drain current of long-channel junctionless (JL) double-gate (DG) field-effect transistors (FET) are presented. A regional method is used to solve the Poisson equation under different gate biases, and the electric potential is obtained. With the potential model, an analytical expression for the threshold voltage is achieved. An expression for the drain current is derived from the potential model. The analytical results are compared with simulations, and excellent agreements are observed. The models accurately describe the characteristics of JLDG FETs, and they are very helpful for the design and optimization of devices.

Fabrication and Characterization of Multiple Herbal Extracts-loaded Nanofibrous Patches for Topical Treatment of Acne Vulgaris

Ying Tang,Lei Liu,Jinju Han,Zhaolun Zhang,Shuyan Yang,Shuxian Li,Shuxian Li,Zhanhua Fan,Hua Zhao 한국섬유공학회 2021 Fibers and polymers Vol.22 No.2

Herbal extracts have recently received considerable interest in acne therapy owing to their enhanced skincompatibility compared with antibiotics and synthetic agents. In this study, electrospun poly (vinyl alcohol)/chitosan (PVA/CS) fibrous patch incorporating a combination of herbal extracts with complementary mechanisms of action was developedas an alternative acne therapy for patients with resistance to the conventional treatments. The herbal extracts-loadedelectrospun fibers displayed uniform and smooth-surfaced nanofibers (dia. 100-300 nm), high drug loading efficiency (89.5-97.7 %) and good water absorbing properties. Results of the in vitro release and antibacterial studies suggest the patches arecapable of sustained release of polyphenol-rich extracts, which may, together with the chitosan present in the fiber, endow thepatch with potent bacterial inhibition of Propionibacterium acnes. The herbal extracts-loaded patches exhibited pronouncedclinical therapeutic efficacy in treating mild-to-moderate facial acne by rapidly reducing inflamed lesions and restoringepidermal barrier. Also, the herbal patches demonstrated improved biocompatibility or tolerability in comparison with thesynthetic drug benzoyl peroxide. Overall, the present study has provided a facile approach to develop biocompatiblepolymers based electrospun fibrous patch for the efficient delivery of herbal extracts for topical skin therapy, opening newperspectives for a new generation of cosmetotextiles.