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RISS 인기검색어
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- 저자 : Shumin Yang (검색결과 3 건)
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Zheng Liu,Shuangcheng Gao,Shumin Zhang,Shangjun Yang,Ning Sun 한국유전학회 2014 Genes & Genomics Vol.36 No.1
An RNAi construct for silencing FtsZ genefunctioning on plastid division in higher plants was transformedinto tobacco genome. Sequencing of the flankingsequences of the T-DNA insertion site in a positive transgenicplant 2-3, which showed wildtype phenotype,revealed that the construct had rearranged, resulting inconvergent transcription of nptII gene by 35S promoter andnos promoter with both transcripts expressing at low level. Although it is possible that the sense and antisense transcriptscan form dsRNA, they did not trigger silencing ofnptII gene derived from a FtsZ silenced plant 2-12 in thecrossed plant 2-392-12. Furthermore, although smallinterfering RNA can trigger DNA methylation of thesilencing locus, our investigation revealed that the crossedplant 2-392-12 showed partial methylation of the nonsilencingtransgene locus but full methylation of the nonrearrangedsilencing locus, suggesting that the efficiency ofRNA-directed DNA methylation is associated with itsoriginal DNA locus.
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Synthesis of thin diamond films from faceted nanosized crystallites
Sobia Allah Rakha,Shumin Yang,Zhoutong He,Ishaq Ahmed,Dezhang Zhu,Jinlong Gong 한국물리학회 2009 Current Applied Physics Vol.9 No.3
Diamond films consist of crystallites having nanometer grains were deposited using low methane concentration by hot filament chemical vapor deposition (HFCVD). The results show that films consist of nanodiamond grains with grain sizes ranging from 20 nm to 200 nm having thickness dependent size. Increasing the deposition time, the grain size increases and hence the hickness of the film increases. The diamond nucleation (nucleation density 1010 cm-2) is comparable to that obtained by biasing the substrate. The use of low methane concentration for the formation of nano crystallites improves the quality of the film as indicated by Raman spectroscopy. The distance between the filament and substrate is increased while maintaining the substrate temperature. The effects of this large separation on the gas phase chemistry are discussed which helps to understand the reduced size of the crystallites under input gas ratios when microcrystallines are obtained. Diamond films consist of crystallites having nanometer grains were deposited using low methane concentration by hot filament chemical vapor deposition (HFCVD). The results show that films consist of nanodiamond grains with grain sizes ranging from 20 nm to 200 nm having thickness dependent size. Increasing the deposition time, the grain size increases and hence the hickness of the film increases. The diamond nucleation (nucleation density 1010 cm-2) is comparable to that obtained by biasing the substrate. The use of low methane concentration for the formation of nano crystallites improves the quality of the film as indicated by Raman spectroscopy. The distance between the filament and substrate is increased while maintaining the substrate temperature. The effects of this large separation on the gas phase chemistry are discussed which helps to understand the reduced size of the crystallites under input gas ratios when microcrystallines are obtained.
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Xia Wang,Hongqin Zhao,Shaonan Yang,Xiaojun Shao,Shumin Nie,Xudong Pan 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.3
Backgrounds: Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) is an lncRNA that has been suggested as a key regulator in the onset of atherosclerosis (AS). This study described the role of MALAT1 in oxidized low density lipoprotein (ox-LDL)-induced endothelial cells death. Methods: Human umbilical vein endothelial cells (HUVECs) were subjected to ox-LDL, before which the expression of MALAT1 was overexpressed by transfection. CCK-8 assay, flow cytometer detection, and western blot were carried out to evaluate cell viability, apoptosis and autophagy. qRT-PCR and western blot analyses were performed to investigate the regulatory relationship between MALAT1, Matrix Gla protein (MGP) and mTOR signaling to decode the underlying mechanism. Results: Up-regulation of MALAT1 attenuated ox-LDLinduced HUVECs lose, as evidenced by the promoted cell viability, and the decreased apoptosis rate. This finding was coupled with the down-regulated p53, Bax, active-caspase-3, Beclin-1 and LC3-II, as well as the up-regulated Bcl-2 and p62. Meanwhile, MALAT1 upregulation promoted the phosphorylation of p70S6K and mTOR, and the expression of MGP. MGP up-regulation exhibited MALAT1-like propoties in preventing ox-LDL-induced cell death and mTOR deactivation. Of contrast, MGP silence affected HUVECs survival and mTOR signaling resulted in contrary impacts. Conclusion: The present work described that MALAT1 up-regulation prevented ox-LDL-mediated apoptosis and autophagy in HUVECs. The protective effects of MALAT1 might be partially via up-regulating MGP, which led to the activation of mTOR signaling.
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