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Shuang Wang,Qian Yang,Zhi-Hua Liu,Lei Sun,Dan Wei,Jun-Zheng Zhang,Jin-Zhu Song,Yun Wang,Jia Song,Jin-Xia Fan,Xian-Xin Meng,Wei Zhang 한국미생물학회 2011 The journal of microbiology Vol.49 No.1
A moderately halophilic bacterial strain 15-13^T, which was isolated from soda meadow saline soil in Daqing City, Heilongjiang Province, China, was subjected to a polyphasic taxonomic study. The cells of strain 15-13^T were found to be Gram-negative, rod-shaped, and motile. The required growth conditions for strain 15-13^T were: 1-23% NaCl (optimum, 7%), 10-50°C (optimum, 35°C), and pH 7.0-11.0 (optimum, pH 9.5). The predominant cellular fatty acids were C18:1 ω7c (60.48%) and C16:0 (13.96%). The DNA G+C content was 67.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain 15-13^T clustered within a branch comprising species of the genus Halomonas. The closest phylogenetic neighbor of strain 15-13^T was Halomonas pantelleriensis DSM 9661^T (98.9% 16S rRNA gene sequence similarity). The level of DNA-DNA relatedness between the novel isolated strain and H. pantelleriensis DSM 9661^T was 33.8%. On the basis of the phenotypic and phylogenetic data, strain 15-13^T represents a novel species of the genus Halomonas, for which the name Halomonas alkalitolerans sp. nov. is proposed. The type strain for this novel species is 15-13^T (=CGMCC 1.9129^T =NBRC 106539^T).
Study on Odor Formation Control during Kraft Pulping
Song HaiNong,Chai XinSheng,Zhu HongXiang,Sung-Hoon Yoon,Zhang Dongcheng,Wang Shuang-Fei 한국펄프·종이공학회 2006 한국펄프종이학회 기타 간행물 Vol.- No.-
The investigation of the formation of organic sulfur compounds (i.e., odor species), mainly methyl mercaptan and demethyl suifide, during kraft pulping has been conducted, in which both hardwood and softwood species were used. It was discovered that there is an organic sulfur compounds formation phase transition point with respect to delignification extent. The transition point occurs at a kappa number of approximately 35 and 20 for softwoods and hardwoods, respectively. The results also showed that both low sulfidity and anthraquinone (AQ) addition are helpful to control the formation of theses organic sulfur compounds during kraft pulping. Whereas Hexenuronic acid (HexA) has contribution to Kappa number, the extended delignification not only lead to low pulp yield, but also be adverse to odor control. A significant in-digester odor reduction can be achieved if the pulping is to be terminated before phase transition point and combined with AQ addition.
Song, Min-Kyu,Cheng, Shuang,Chen, Haiyan,Qin, Wentao,Nam, Kyung-Wan,Xu, Shucheng,Yang, Xiao-Qing,Bongiorno, Angelo,Lee, Jangsoo,Bai, Jianming,Tyson, Trevor A.,Cho, Jaephil,Liu, Meilin American Chemical Society 2012 Nano letters Vol.12 No.7
<P>While pseudocapacitors represent a promising option for electrical energy storage, the performance of the existing ones must be dramatically enhanced to meet today’s ever-increasing demands for many emerging applications. Here we report a nanostructured, mixed-valent manganese oxide film that exhibits anomalously high specific capacitance (∼2530 F/g of manganese oxide, measured at 0.61 A/g in a two-electrode configuration with loading of active materials ∼0.16 mg/cm<SUP>2</SUP>) while maintaining excellent power density and cycling life. The dramatic performance enhancement is attributed to its unique mixed-valence state with porous nanoarchitecture, which may facilitate rapid mass transport and enhance surface double-layer capacitance, while promoting facile redox reactions associated with charge storage by both Mn and O sites, as suggested by in situ X-ray absorption spectroscopy (XAS) and density functional theory calculations. The new charge storage mechanisms (in addition to redox reactions of cations) may offer critical insights to rational design of a new-generation energy storage devices.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2012/nalefd.2012.12.issue-7/nl300984y/production/images/medium/nl-2012-00984y_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl300984y'>ACS Electronic Supporting Info</A></P>
Shuang Mi,Jian Song,Yuanyuan Che,Huajun Zheng,Jianqiang Lin,Jianqun Lin 한국미생물학회 2011 The journal of microbiology Vol.49 No.6
Leptospirillum ferriphilum has been identified as the dominant, moderately thermophilic, bioleaching microorganism in bioleaching processes. It is an acidic and chemolithoautrophic bacterium that gains electrons from ferrous iron oxidation for energy production and cell growth. Genetic information about this microorganism has been limited until now, which has hindered its further exploration. In this study, the complete genome of L. ferripilum ML-04 is sequenced and annotated. The bacterium has a single circular chromosome of 2,406,157 bp containing 2,471 coding sequences (CDS), 2 rRNA operons, 48 tRNA genes, a large number of mobile genetic elements and 2 genomic islands. In silico analysis shows L. ferriphilum ML-04 fixes carbon through a reductive citric acid (rTCA) cycle, and obtains nitrogen through ammonium assimilation. The genes related to “cell envelope biogenesis, outer membrane” (6.9%) and “DNA replication, recombination and repair” (5.6%) are abundant, and a large number of genes related to heavy metal detoxification, oxidative and acidic stress defense, and signal transduction pathways were detected. The genomic plasticity, plentiful cell envelope components, inorganic element metabolic abilities and stress response mechanisms found the base for this organism’s survival in the bioleaching niche.
Shuang Liu,Huishan Qin,Yanmin Su,Jiali Li,Wenjing Cao,Wen He,Zhen Zeng,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To investigate the effect of TSS on lipid metabolism and oxidative stress in HFD mice. The body weight and food intake were observed. The serum SOD, MDA, GSH, TC, TG, HDL-C, LDL-C were determined by kits. Western Blotting was used to detect the levels of PPAR-γ, AMPK, SIRT1 and PGC-1α. TSS treatment reduced the body weight, Lee"s index and fat organ indices of mice in the HFD group. Compared with the control(CON) group, the serum TC, TG and LDL-C in the HFD group were increased, Administrated with TSS can improve abnormal blood lipid levels. Compared with the CON group, the serum SOD level in HFD group was significantly reduced(P<0.05), and MDA level was increased; while the levels of serum MDA in the TSS group decreased and SOD level increased. The pathological sections showed that TSS could improve the degree of hepatic steatosis. TSS also increased the levels of PPAR-γ, AMPK, SIRT1 and PGC-1α, and the effect of the high-dose group was the most significant. TSS can reduce body weight and fat accumulation, improve lipid metabolism disorder and oxidative stress caused by HFD.
Characterization of microbiota of naturally fermented sauerkraut by high-throughput sequencing
Shuang Zhang,Yichen Zhang,Lihong Wu,Lili Zhang,Song Wang 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.6
Sauerkraut is a traditionally fermented cabbage, with a unique taste and beneficial properties, in northeast China. The taste and flavor of sauerkraut vary from region to region, owing to the differences in microorganisms. Illumina MiSeq sequencing was used to identify and quantify the microbial community composition of the broth and leaves of the naturally fermented Suan-cai collected from northeast China. The alpha and beta diversity of the samples from three areas in Heilongjiang province shown that the complexity of bacterial diversity of the three samples was C, A and B in turn. The Lactobacillus widely existed in fermented sauerkraut, of these, Latilactobacillus sakei, Loigolactobacillus coryniformis subsp. torquens, Lactiplantibacillus plantarum subsp. plantarum, and Secundilactobacillus malefermentans were more abundant in the sauerkraut leaves than in fermentation broth. Other genera of lactic acid bacteria Pediococcus and Leuconostoc, which have potential probiotic properties, were also present. However, some harmful bacteria such as Arcobacter and Acinetobacter were also detected.
Functional Roles of Exosomes in Allergic Contact Dermatitis
Song Bocui,Chen Qian,Li Yuqi,Zhan Shuang,Zhao Rui,Shen Xue,Liu Min,Tong Chunyu 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.12
Allergic contact dermatitis (ACD) is an allergen-specific T-cell-mediated inflammatory response, albeit with unclear pathogenesis. Exosomes are nanoscale extracellular vesicles secreted by several cell types and widely distributed in various biological fluids. Exosomes affect the occurrence and development of ACD through immunoregulation among other ways. Nevertheless, the role of exosomes in ACD warrants further clarification. This review examines the progress of research into exosomes and their involvement in the pathogenesis, diagnosis, and treatment of ACD and provides ideas for exploring new diagnostic and treatment methods for this disease.
Shuangli Liu,Xiaohui Wang,Rui Zhang,Mingjie Song,Nanqi Zhang,Wanying Li,Yingping Wang,Yonghua Xu,Lianxue Zhang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.4
Background: Previous studies have shown the insecticidal efficacy of ginsenosides. In the present study,we aimed to investigate the metabolic mechanism related to the inhibitory effect of panaxadiol saponins(PDSs) against the Asian corn borer Ostrinia furnacalis (Guenee). Methods: Third instar larvae of O. furnacalis were fed normal diets with different concentrations of PDSsfor 4 days. The consumption index, relative growth rate, approximate digestibility, and conversion ofingested and digested food were recorded. A targeted gas chromatographyemass spectrometry assaywas performed to detect the profiles of amino acids, fatty acids, and carbohydrates in larvae ofO. furnacalis. In addition, the activity of detoxification-related enzymes was determined. Results and Conclusions: PDSs decreased the consumption index, relative growth rate, approximate digestibility,and conversion of ingested and digested food in the 3rd instar larvae of O. furnacalis in a dosedependentmanner. PDSs decreased 15 free amino acids, 16 free fatty acids, and 5 carbohydrates andincreased the levels of palmitoleic acid, palmitic acid, and 9-octadecenoic acid in the 3rd instar larvae. The activity of detoxification-related enzymes, such as acetylcholinesterase, glutathione S-transferase,cytochrome P450, carboxylesterase, trehalase, acid phosphatase, and alkaline phosphatase, was reducedin a dose-dependent manner in the 3rd instar larvae exposed to PDSs. These data confirmed theinhibitory effect of PDSs against growth, food utilization, and detoxification in the 3rd instar larvae ofO. furnacalis and the potential for using PDSs as an efficient tool for insect pest management forO. furnacalis larvae.