Non-thermal plasma enhances rice seed germination, seedling development, and root growth under low-temperature stress

Bian Jing-Yang,Guo Xiao-Yu,Lee Dong Hun,Sun Xing-Rong,Liu Lin-Shuai,Shao Kai,Liu Kai,Sun Hu-Nan,Kwon Taeho 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

Recently, non-thermal plasma (NTP) technologies have found widespread application across diverse fields, including plant growth, medical science, and biological and environmental research. Rice (Oryza sativa L.) is exceptionally sensitive to temperature changes. Notably, low-temperature stress primarily affects the germination and reproductive stages of rice, often leading to reduced crop yield. This study aimed to identify optimal conditions for enhancing rice seed germination and seedling growth under low temperatures using NTP technology. Our research indicated that NTP treatment at 15.0 kV for 30 s optimally promotes rice seed germination and growth under low-temperature stress. Furthermore, NTP treatment increases the activity and expression of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), under low-temperature conditions. Moreover, it downregulates the expression of β-ketoacyl-[acyl carrier protein] synthase I (KASI) and cis-epoxy carotenoid dioxygenase 3 (NCED3) and upregulates the expression of alternative oxidase (AOX1B), BREVIS RADIX-like homologous gene (BRXL2), WRKY transcription factor 29 (WRKY29), and EREBP transcription factor 2 (EREBP2) in roots after tandem 7 days lowtemperature (16 ℃) and 7 days room-temperature (28 ℃) treatments. Transcriptomic analysis revealed the involvement of various key genes in phosphotransferase activity, phosphate-containing compound metabolic processes, and defense responses. These analyses provide comprehensive information on gene expression at the transcriptional level, offering new insights for a deeper understanding of candidate genes required for root growth in rice.

Haemaphysalis concinna (Acari: Ixodida): Persistent efficacy of doramectin in rabbits under laboratory conditions

Yao BIAN,Guangyou YANG,Tao WANG,Huijuan YAN,Shuai WANG,Jiagang SUN,Kaijun LI 한국곤충학회 2009 Entomological Research Vol.39 No.2

In the present study, the persistent efficacy of doramectin (DOR) in rabbits infested by Haemaphysalis concinna was observed. DOR (200 µg/kg) was administered once to infested rabbits by subcutaneous injection. A total of 15 rabbits were allocated to three groups, which were observed for efficacy. On days 1–7, 8–14, 15–21 and 22–28 post-inoculation, the percent reduction of tick larvae in DOR treated rabbits was 100.0, 100.0, 86.7 and 61.7%, respectively; the percent reduction of nymphs was 100.0, 90.0, 75.0 and 65.0%, respectively; and the percent reduction of adults was 85.0, 65.0, 55.0 and 55.0%, respectively. In comparison, in untreated rabbits at the same time points (control group), the percent reduction of larvae was 5.0, 3.3, 5.0 and 5.0%, respectively, and there was no reduction of nymphs and adults in the untreated rabbits.

Regulation of matrix metalloproteinase-9 protein expression by 1α , 25-(OH)2D3 during osteoclast differentiation

Jian-Hong Gu,Xi-Shuai Tong,Guohong Chen,Xue-Zhong Liu,Jian-Chun Bian,Yan Yuan,Zong-Ping Liu 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.1

To investigate 1α,25-(OH)2D3 regulation of matrixmetalloproteinase-9 (MMP-9) protein expression duringosteoclast formation and differentiation, receptor activator ofnuclear factor κB ligand (RANKL) and macrophagecolony-stimulating factor (M-CSF) were administered toinduce the differentiation of RAW264.7 cells into osteoclasts. The cells were incubated with different concentrations of1α,25-(OH)2D3 during culturing, and cell proliferation wasmeasured using the methylthiazol tetrazolium method. Osteoclast formation was confirmed using tartrate-resistantacid phosphatase (TRAP) staining and assessing bone lacunarresorption. MMP-9 protein expression levels were measuredwith Western blotting. We showed that 1α,25-(OH)2D3inhibited RAW264.7 cell proliferation induced by RANKLand M-CSF, increased the numbers of TRAP-positiveosteoclasts and their nuclei, enhanced osteoclast boneresorption, and promoted MMP-9 protein expression in aconcentration-dependent manner. These findings indicatethat 1α,25-(OH)2D3 administered at a physiological relevantconcentration promoted osteoclast formation and couldregulate osteoclast bone metabolism by increasing MMP-9protein expression during osteoclast differentiation.

Mechanical Properties and Damage Characteristics of Coal-Rock Combination with Different Dip Angles

Zhi-Guo Xia,Shuai Liu,Zhuang Bian,Jinhu Song,Fan Feng,Ning Jiang 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5

The effect of the dip angle of an interlayer coal seam on rock mass mechanical behavior and damage characteristics is seldom discussed. To address the lack of the existing research, a numerical simulation was conducted using the Particle Flow Code (PFC2D), and its damage to mechanical behavior, acoustic emission (AE) characteristics, and damage characteristics were analyzed. The outcomes exhibit the peak strength and elastic modulus of coal-rock combinations are between that of rock and coal. As the coal inclination angle rises, the elastic modulus and peak strength gradually decrease. AE model experiences the initial growth period, stable growth period, and rapid decline period. The value of AE hits and the generation of the AE signal is affected by the size of the inclination angle. The degree of damage to the coal-rock combination gradually rises as the angle rises. The damage process of the coal and rock composite sample encompasses an initial damage phase, damage stable evolution phase, accelerated damage phase, and a failure phase. The damage values of the stable evolution stage for varying dip angle models are also different. As the inclination angle increases, the damage value gradually increases, while the strain at the same damage value gradually decreases.

Bevacizumab Regulates Cancer Cell Migration by Activation of STAT3

Wu, Huan-Huan,Zhang, Shuai,Bian, Huan,Li, Xiao-Xu,Wang, Lin,Pu, Yin-Fei,Wang, Yi-Xiang,Guo, Chuan-Bin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

There are numerous clinical cases indicating that long-term use of bevacizumab may increase the invasiveness of tumors. However, to date, little is known about underlying molecular mechanisms. Therefore, the purpose of our study was to investigate effects of bevacizumab in four cancer cells lines (WSU-HN6, CAL27, Tca83, and HeLa). It was found to promote migration and invasion in the WSU-HN6 and Tca83 cases, while exerting inhibitory effects in CAL27 and HeLa cells. The signal transducer and activator of transcription (STAT) 3 inhibitors niclosamide and S3I-201 inhibited the STAT3 signal pathway, which is activated by bevacizumab. These inhibitors also substantially blocked bevacizumab-induced migration of WSU-HN6 and Tca83 cells. Bevacizumab upregulated interleukin (IL)-6 and phosphorylated (p)-STAT3 expression time-dependently. Therefore, we propose that bevacizumab has differential effects on the migration of different cancer cell lines and promotes migration via the IL-6/STAT3 signaling pathway.

Ginseng-derived nanoparticles induce skin cell proliferation and promote wound healing

Song Yang,Shuyan Lu,Limei Ren,Shuai Bian,Daqing Zhao,Meichen Liu,Jiawen Wang 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.1

De novo transcriptome sequencing and gene expression profiling of sweetpotato leaves during low temperature stress

Pan Jiaquan,Zhang Qian,Liu Shuai,Liu Zhiqiang,Bian Xiaofeng,Yu Tao 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.6

Sweetpotato [Ipomoea batatas (L.) Lam] is one of the most important crops for food, feed, industrial material, and bio-energy resource. However, since it originated in tropical regions, sweetpotato exhibits limited cold tolerance, hindering its cultivation in low-temperature areas. To comprehend the sweetpotato response to chilling stress, de novo transcriptome assembly was conducted on leaves from two sweetpotato cultivars: the cold-tolerant Liaohanshu 21 and the cold-sensitive Shenshu 28, exposed to low-temperature stress for 3 h and 24 h. 787.24 million raw reads and 763.44 million clean reads were obtained, 60.60–66.06% of clean reads successfully mapped to the reference database across all 18 libraries. 2524 unigenes were identified as transcript factors and classified into 55 families in Plant Transcription Factor Database (PlantTFDB) and 4671 unigenes were matched to 24 groups in Plant Resistance Gene Database (PRGdb). In Liaohanshu 21, 3618 genes and 8391 genes exhibited significant differential expression 3 h and 24 h of low-temperature exposure, respectively. In Shenshu 28, 13,394 genes and 11,627 genes displayed substantial differential expression after 3 h and 24 h of low-temperature exposure, respectively. The differentially expressed genes (DEGs) were further analyzed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genome (KEGG) database. DEGs related to “active oxygen scavenging systems”, “lipid metabolism”, and “plant hormone signal transduction” were potentially associated with sweetpotato’s cold resistance. This research offers insights into the mechanisms and genes involved in cold stress in sweetpotato’s response to cold stress and will contribute valuable information for cold-tolerant breeding of sweetpotato.

Inhibitory effects of osteoprotegerin on osteoclast formation and function under serum-free conditions

Ying-Xiao Fu,Jian-Hong Gu,Yi-Ran Zhang,Xi-Shuai Tong,Hong-Yan Zhao,Yan Yuan,Xue-Zhong Liu,Jian-Chun Bian,Zong-Ping Liu 대한수의학회 2013 Journal of Veterinary Science Vol.14 No.4

The purpose of this study was to determine whether osteoprotegerin (OPG) could affect osteoclat differentiation and activation under serum-free conditions. Both duck embryo bone marrow cells and RAW264.7 cells were incubated with macrophage colony stimulatory factor (M-CSF) and receptor activator for nuclear factor κB ligand (RANKL) in serum-free medium to promote osteoclastogenesis. During cultivation, 0,10, 20, 50, and 100 ng/mL OPG were added to various groups of cells. Osteoclast differentiation and activation were monitored via tartrate-resistant acid phosphatase (TRAP) staining,filamentous-actin rings analysis, and a bone resorption assay. Furthermore, the expression osteoclast-related genes, such as TRAP and receptor activator for nuclear factor κB (RANK),that was influenced by OPG in RAW264.7 cells was examined using real-time polymerase chain reaction. In summary,findings from the present study suggested that M-CSF with RANKL can promote osteoclast differentiation and activation,and enhance the expression of TRAP and RANK mRNA in osteoclasts. In contrast, OPG inhibited these activities under serum-free conditions.