Anticancer Properties of Total Alkaloid fraction of Solanum pseudocapsicum unripe fruits

Badami, Shrishailappa,Dhonde, Satish G.,Reddy SA, Manohar,P., Vijayan,B, Suresh Kyung Hee Oriental Medicine Research Center 2004 Oriental pharmacy and experimental medicine Vol.4 No.1

The total alkaloid fraction of the methanolic extract of Solanum pseudocapsicum unripe fruits was tested for its in-vivo anticancer activity against Dalton's Lymphoma Ascites model in mice. The total alkaloid fraction at 5.0 and 10.0 mg/kg body weight showed significant increase in the mean survival time and the percentage increase in the life span of tumor bearing mice. The increase in the body weight was found to be less than that of the control. However, the treatment at 20 mg/ kg body weight was found to be toxic and showed a decrease in the mean survival time, and body weight when compared to control mice. The antitumor activity observed may be due to the cytotoxic activity of the total alkaloid fraction.

Antioxidant activity of Grewia tiliaefolia bark

Badami, Shrishailappa,Gupta, Mahesh Kumar,M., Ramanathan,B., Suresh Kyung Hee Oriental Medicine Research Center 2005 Oriental pharmacy and experimental medicine Vol.5 No.1

Grewia tiliaefolia is commonly used in India and several other countries for its ethnomedical properties. The antioxidant effect of 50% methanol extract of Grewia tiliaefolia bark was investigated by in-vitro and in-vivo methods. The extract exhibited $IC_{50}$ value of $51.40{\pm}3.98\;{\mu}g/ml$ in DPPH assay. In the in vivo experiments, the treatment was given at 250 and 500 mg/kg body weight doses orally for seven days and catalase, SOD, TBA-RS and ascorbic acid levels were estimated. Both the doses caused a significant increase in the levels of catalase in the liver and kidney, and SOD in the liver, kidney and blood serum. A significant decrease in the level of TBARS was also observed. These results suggest potent antioxidant nature of the extract.

In vitro antioxidant activity of various extracts of Aristolochia bracteolata leaves

Badami, Shrishailappa,Jose, Christy K,K, Choksi Rakshit Kumar,Dongre, Santosh Kumar H,PC, Jagadish,B, Suresh Kyung Hee Oriental Medicine Research Center 2005 Oriental pharmacy and experimental medicine Vol.5 No.4

Aristolochia bracteolata is being used in traditional medicine for a variety of ailments. Three successive and two crude extracts of its leaves were subjected for in vitro antioxidant activity using seven different methods. The successive ethyl acetate and crude methanol extracts have shown potent antioxidant activity in ABTS method with $IC_{50}$ values of $17.08\;{\pm}\;0.44\;and\;28.12\;{\pm}\;2.87\;{\mu}g/ml$, respectively. The crude methanol and successive chloroform extracts have shown moderate antioxidant activity in scavenging of hydroxyl radical by p-NDA method. All the extracts have shown moderate to low antioxidant activity by other methods.

Cytotoxic and antioxidant properties of four plants belonging to the genus Solanum

Dongre, Santoshkumar H.,Badami, Shrishailappa,Godavarthi, Ashok,S., Mahendran,P., Vijayan,B., Suresh Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.1

The aim of the study was to evaluate in vitro antioxidant and cytotoxic activities of the methanolic extracts of leaves of Solanum sisymbrifolium, Solanum anguivi multiflora, Solanum barbisetum and Solanum jasminoides. In the in vitro antioxidant screening using ABTS [2,2'-azino-bis (3-ethylbenzo-thiazoline-6-sulphonic acid) diammonium salt] method, the methanol extracts of Solanum jasminoides, Solanum barbisetum and Solanum anguivi multiflora exhibited potent antioxidant activity with $IC_{50}$ values 31.25 $\pm$ 0.35, 40.33 $\pm$ 0.57 and 54.33 $\pm$ 0.57 ${\mu}g$/ml, respectively. Solanum barbisetum also showed potent activity in DPPH [1,1-diphenyl-2-picryl hydrazyl] method with an $IC_{50}$ value of 55.33 $\pm$ 1.66 ${\mu}g/ml. In the cytotoxicity studies, the methanol extract of Solanum barbisetum exhibited moderate activity against Vero, HEp-2, HeLa and A-549 cell lines with $IC_{50}$ values in the range of 83.30 - 127.30 ${\mu}g$/ml. Solanum anguivi multiflora extract also showed moderate activity against Hep-2 cell line with $IC_{50}$ value of 80.13 ${\mu}g$/ml. Solanum barbisetum possessing both the activities requires further investigation.

Cytotoxic and antioxidant properties of four plants belonging to the genus Solanum

Santoshkumar H Dongre,Shrishailappa Badami,Ashok Godavarthi, Mahendran S,Vijayan P andSuresh B 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.1

The aim of the study was to evaluate in vitro antioxidant and cytotoxic activities of the methanolic extracts of leaves of Solanum sisymbrifolium, Solanum anguivi multiflora, Solanum barbisetum and Solanum jasminoides. In the in vitro antioxidant screening using ABTS [2,2’-azino-bis (3-ethylbenzo-thiazoline-6- sulphonic acid) diammonium salt] method, the methanol extracts of Solanum jasminoides, Solanum barbisetum and Solanum anguivi multiflora exhibited potent antioxidant activity with IC50 values 31.25 ± 0.35, 40.33 ± 0.57 and 54.33 ± 0.57 μg/ml, respectively. Solanum barbisetum also showed potent activity in DPPH [1,1-diphenyl-2-picryl hydrazyl] method with an IC50 value of 55.33 ± 1.66 μg/ml. In the cytotoxicity studies, the methanol extract of Solanum barbisetum exhibited moderate activity against Vero, HEp-2, HeLa and A-549 cell lines with IC50 values in the range of 83.30 - 127.30 μg/ml. Solanum anguivi multiflora extract also showed moderate activity against Hep-2 cell line with IC50 value of 80.13 μg/ml. Solanum barbisetum possessing both the activities requires further investigation. The aim of the study was to evaluate in vitro antioxidant and cytotoxic activities of the methanolic extracts of leaves of Solanum sisymbrifolium, Solanum anguivi multiflora, Solanum barbisetum and Solanum jasminoides. In the in vitro antioxidant screening using ABTS [2,2’-azino-bis (3-ethylbenzo-thiazoline-6- sulphonic acid) diammonium salt] method, the methanol extracts of Solanum jasminoides, Solanum barbisetum and Solanum anguivi multiflora exhibited potent antioxidant activity with IC50 values 31.25 ± 0.35, 40.33 ± 0.57 and 54.33 ± 0.57 μg/ml, respectively. Solanum barbisetum also showed potent activity in DPPH [1,1-diphenyl-2-picryl hydrazyl] method with an IC50 value of 55.33 ± 1.66 μg/ml. In the cytotoxicity studies, the methanol extract of Solanum barbisetum exhibited moderate activity against Vero, HEp-2, HeLa and A-549 cell lines with IC50 values in the range of 83.30 - 127.30 μg/ml. Solanum anguivi multiflora extract also showed moderate activity against Hep-2 cell line with IC50 value of 80.13 μg/ml. Solanum barbisetum possessing both the activities requires further investigation.

Selective in vitro cytotoxicity of Hypericum hookerianum towards cancer cell lines

P., Vijayan,S., Vinod Kumar,Badami, Shrishailappa,PK, Mukherjee,SA, Dhanaraj,B., Suresh Kyung Hee Oriental Medicine Research Center 2003 Oriental pharmacy and experimental medicine Vol.3 No.3

The methanol extracts of the aerial parts, leaves and stem of Hypericum hookerianum were tested for in vitro cytotoxicity on selected normal and cancer cell lines and anti tumor activity using DLA cells. Cell viability and morphological changes were assessed. Among the three extracts tested, the stem extract of Hypericum hookerianum showed potent cytotoxicity against HEp-2 and RD cell lines. The $CTC_{50}$(concentration required to reduce viability by 50%) of this extract was found to be $2.02\;{\mu}g/ml$ for RD cell line, $10.25\;{\mu}g/ml$ for HEp-2 cell line and $100.06\;{\mu}g/ml$ for Vero cell line. In the clonogenic assay, no colony formation was observed up to a concentration of $100\;{\mu}g/ml$. In the short term cytotoxicity studies using DLA cells, 50% viability was observed in the concentration range of $50-100\;{\mu}g/ml$ for aerial parts, $100-200\;{\mu}g/ml$ for stem and more than $200\;{\mu}g/ml$ for leaf extracts of Hypericum hookerianum. In the long-term activity using HEp-2 cell line, no colony formation was observed over a concentration of 200 mg/ml for the stem extract. Hypericum hookerianum stem extract was fractionated into petroleum ether, chloroform, ethyl acetate and methanol soluble fractions. The petroleum ether and chloroform soluble fractions showed higher cytotoxic activity against HEp-2 cell line when compared to the other two fractions. The methanol stem extract of Hypericum hookerianum has the potential for further investigation in animal models to determine its anti-tumor activity and to identify its active principles.