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Qinfang Lu,Byung-gon Jeong,Ping Yan,Sohee Kim,Shirong Lai,Jiancheng Liu 대한환경공학회 2020 대한환경공학회지 Vol.42 No.11
Objectives : Wastewater produced by fatty acid production contains high concentration of organic substances and high concentration of salts (mainly sodium sulfate), causing great pollution to water resources and environment. The pollution prevention and control of this type of wastewater are very necessary. The key to treating this type of wastewater is to remove salts and COD to achieve harmless treatment. This is a problem in wastewater management that has plagued the industry for a long time. This paper proposed a technique suitable for fatty acid high salinity organic wastewater. Methods : First, the industrial treatment technology of organic wastewater with high salinity was introduced and analyzed. Combined with the principle of industrial wastewater treatment, the process route for the treatment of fatty acid high salinity organic wastewater was analyzed and selected. In addition, the key technology and process for anaerobic desalination and COD removal were analyzed and selected. Results and Discussion : According to the unique nature of this type of wastewater mainly containing sulphate salts and the feasibility of industrial production, a special technology combination was proposed to treat this wastewater at this stage. Since this wastewater has a B/C ratio of 0.4 to 0.45, it is easier to use biological treatment method. Thus, the conventional treatment method is pretreatment + biological treatment. Biological enhancement and reactor process optimization can be studied for better efficiency. Conclusions : Considering the high COD and sulphate concentration characteristics of fatty acid high-salinity organic wastewater, high-efficiency anaerobic biochemical treatment is mainly considered. Combined with modern high-efficiency anaerobic suspended sludge granule technology, it was concluded that pretreatment + high efficiency IC anaerobic + secondary biological treatment can achieve industrialized treatment of such wastewater in a targeted, low-cost and reliable way. In the later stage, bio-enhancement of the anaerobic process as well as structural and process optimization of the reactor can be carried out to obtain better technical and economic results in production practice.
An Oligonucleotide Microarray Bait for Isolation of Target Gene Fragments
Shi, Rong,Ma, Wen-li,Liu, Cui-Hua,Song, Yan-Bin,Mao, Xiang-Ming,Zheng, Wen-Ling Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.2
A new molecular-baiting method was studied by retrieving targeted gene fragments from an oligonucleotide microarray bait after hybridization. To make the microarray bait, 70-mer oligonucleotides that were designed to specifically represent the SSA1 gene of Saccharomyces cerevisiae were printed on the slide. Samples of the Saccharomyces cerevisiae mRNA were extracted and labeled by the RD-PCR (Restriction Display PCR) method using the Cy5-labelled universal primer, then applied for hybridization. The sample fragments that hybridized to the microarray were stripped, and the eluted cDNAs were retrieved and cloned into the pMD 18-T vector for transformation, plasmid preparation, and sequencing. BLAST searching of the GenBank database identified the retrieved fragments as being identical to the SSA1 gene (from 2057-2541bp). A new method is being established that can retrieve the sample fragments using an oligo-microarray-bait.
An Oligonucleotide Microarray Bait for Isolation of Target Gene Fragments
( Shi Rong ),( Ma Wen Li ),( Liu Cui Hua ),( Song Yan Bin ),( Mao Xiang Ming ),( Zheng Wen Ling ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.2
A new molecular-baiting method was studied by retrieving targeted gene fragments from an oligonucleotide microarray bait after hybridization. To make the microarray bait, 70-mer oligonucleotides that were designed to specifically represent the SSAl gene of Saccharomyces cerevisiae were printed on the slide. Samples of the Saccharomyces cerevisiae mRNA were extracted and labeled by the RD-PCR (Restriction Display PCR) method using the CyS-labelled universal primer, then applied for hybridization. The sample fragments that hybridized to the microarray were stripped, and the eluted cDNAs were retrieved and cloned into the pMD 18-T vector for transformation, plasmid preparation, and sequencing. BLAST searching of the GenBank database identified the retrieved fragments as being identical to the SSAl gene (from 2057-2541bp). A new method is being established that can retrieve the sample fragments using an oligo-microarray-bait.