Oligopeptide derived from solid-state fermented cottonseed meal significantly affect the immunomodulatory in BALB/c mice treated with cyclophosphamide

Jiancheng Liu,Hong Sun,Cunxi Nie,Wenxia Ge,Yongqiang Wang,Wen-Ju Zhang 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.6

In this study, the immunomodulatory activity of oligopeptide (CP) derived from solid-state fermented cottonseed meal were investigated in immunosuppressed BALB/c mice models by treatment with cyclophosphamide (CY). Results indicated that oligopeptide increased the thymus and spleen indices of CY-treated mice. The count of plague forming cells (PFC) and the content of half serum hemolysis (HC50) in immunosuppressive mice were restored to the normal level in CP-10 and CP-20 groups while the cytokines interleukin (IL)-2, IL-6, and tumor necrosis factor alpha (TNF-a) were increased significantly in CP-20 group. Similar increasing the immunoglobulin of IgG and IgM content in the serum of CP-10 group were also observed. These findings indicated that oligopeptide derived from solid-state fermented cottonseed meal had a strong immune-enhancing activity as well as a protective effect against immunosuppression induced by cyclophosphamide in mice.

HYPERSURFACES WITH CONSTANT k-TH MEAN CURVATURE AND TWO DISTINCT PRINCIPAL CURVATURES IN SPHERES

Liu, Jiancheng,Wei, Yan Korean Mathematical Society 2012 대한수학회보 Vol.49 No.2

In this paper, we investigate the hypersurface M in a unit sphere with constant k-th mean curvature and two distinct principal curvatures, and characterize such a hypersurface.

COMPLETE SPACELIKE HYPERSURFACES WITH CMC IN LORENTZ EINSTEIN MANIFOLDS

Liu, Jiancheng,Xie, Xun Korean Mathematical Society 2021 대한수학회보 Vol.58 No.5

We investigate the spacelike hypersurface Mⁿ with constant mean curvature (CMC) in a Lorentz Einstein manifold Lⁿ⁺¹₁, which is supposed to obey some appropriate curvature constraints. Applying a suitable Simons type formula jointly with the well known generalized maximum principle of Omori-Yau, we obtain some rigidity classification theorems and pinching theorems of hypersurfaces.

Hypersurfaces with constant $\boldsymbol{k}$-th mean curvature and two distinct principal curvatures in spheres

Jiancheng Liu,Yan Wei 대한수학회 2012 대한수학회보 Vol.49 No.2

In this paper, we investigate the hypersurface $M$ in a unit sphere with constant $k$-th mean curvature and two distinct principal curvatures,and characterize such a hypersurface.

Circ_0001686 Promotes Prostate Cancer Progression by Up-Regulating SMAD3/TGFBR2 via miR-411-5p

Pan Jiancheng,Liu Zihao,Yang Zhizhao,Liang Enli,Fang Cheng,Zhang Dingrong,Zhou Xiaodong,Niu Yuanjie,Xin Zhongcheng,Chen Yegang,Cai Qiliang 대한남성과학회 2022 The World Journal of Men's Health Vol.40 No.1

Purpose: As the mechanism of interaction between circular RNAs (circRNAs) and microRNAs (miRNAs) in regulating the development of prostate cancer (PCa) is not clear, this study focuses on investigating these effects. Materials and Methods: Sample tissues were collected from the PCa of patients, and microarray analysis of human circRNAs was conducted. The expression of circ_0001686, hsa_miR-411-5p (miR-411-5p) were also detected by qRT-PCR. Circ_0001686 and miR-411-5p mimics were transfected into the PCa cell lines (CWR22RV1and LNCaP) and MTT, colony formation, Transwell, and scratch wound assays were used to analyze the biological behaviors of PCa cells. Si-circ_0001686 and ASO-miR-411-5p were used as negative controls, and dual-luciferase reporter assays were performed to verify the interactions among circ_0001686, miR-411-5p, and SMAD3/TGFBR2. The levels of SMAD3 and TGFBR2 in different treated PCa cells were measured by western blot, and in vivo experiments in a nude mouse model were carried out to strengthen the in vitro findings of miR-411-5p. Results: The expression of circ_0001686 was up-regulated, while the expression of miR-411-5p was down-regulated in PCa cells. Moreover, circ_0001686 promoted cell proliferation, migration, and invasion. Molecular mechanism exploration revealed that circ_0001686 could reduce miR-411-5p, affecting the downstream target genes of SMAD3 and TGFBR2. In vitro and in vivo studies verified that miR-411-5p inhibits PCa progression. Conclusions: Circ_0001686 can reduce miR-411-5p to increase the expression of SMAD3/TGFBR2, which consequently promotes the proliferation, invasion, and migration of PCa cells.

Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husks

Wei Huan,Liu Jiancheng,Liu Mengjian,Zhang Huiling,Chen Yong 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.4

Objective: This study aimed to assess the impact of a hydroethanolic extract of walnut green husks (WGH) on rumen fermentation and the diversity of bacteria, methanogenic archaea, and fungi in sheep fed a high-concentrate diet. Methods: Five healthy small-tailed Han ewes with permanent rumen fistula were selected and housed in individual pens. This study adopted a self-controlled and crossover design with a control period and an experimental period. During the control period, the animals were fed a basal diet (with a ratio of concentrate to roughage of 65:35), while during the treatment period, the animals were fed the basal diet supplemented with 0.5% hydroethanolic extract of WGH. Fermentation parameters, digestive enzyme activities, and microbial diversity in rumen fluid were analyzed. Results: Supplementation of hydroethanolic extract of WGH had no significant effect on feed intake, concentrations of total volatile fatty acids, isovalerate, ammonia nitrogen, and microbial protein (p>0.05). However, the ruminal pH, concentrations of acetate, butyrate and isobutyrate, the ratio of acetate to propionate, protozoa count, and the activities of filter paper cellulase and cellobiase were significantly increased (p<0.05), while concentrations of propionate and valerate were significantly decreased (p<0.05). Moreover, 16S rRNA gene sequencing revealed that the relative abundance of rumen bacteria Christensenellaceae R7 group, Saccharofermentans, and Ruminococcaceae NK4A214 group were significantly increased, while Ruminococcus gauvreauii group, Prevotella 7 were significantly decreased (p<0.05). The relative abundance of the fungus Pseudomonas significantly increased, while Basidiomycota, Fusarium, and Alternaria significantly decreased (p<0.05). However, there was no significant change in the community structure of methanogenic archaea. Conclusion: Supplementation of hydroethanolic extract of WGH to a high-concentrate diet improved the ruminal fermentation, altered the structure of ruminal bacterial and fungal communities, and exhibited beneficial effects in alleviating subacute rumen acidosis of sheep.

Extraction of visual texture features of seabed sediments using an SVDD approach

Li, Yan,Liu, Shijie,Zhu, Puqiang,Yu, Jiancheng,Li, Shuo Pergamon Press 2017 Ocean engineering Vol.142 No.-

<P><B>Abstract</B></P> <P>Perception of the seabed environment is an important capability of autonomous underwater vehicles. This paper focuses on defining and extracting robust texture features from visual images that lead to useful and practical automated identification of the types of seabed sediments. The visual texture features are described by using a gray-level co-occurrence matrix (GLCM) and fractal dimension, after which an unsupervised learning method, self-organizing map (SOM), is adopted to evaluate the validity of features descriptors on three types of seabed sediments. Subsequently, a kernel-based approach that exhibits robustness versus low numbers of high-dimensional samples, named support vector domain description (SVDD), is applied to classify the types of seabed sediments. In comparison with state-of-the-art classifiers, the experimental results demonstrated the effectiveness of the SVDD on the classification of seabed sediments.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The visual images of seabed sediments are characterized by the texture features which are extracted based on the GLCM and fractal theory. </LI> <LI> A multi-class classification strategy for seabed sediments is proposed by adding a distance measure after SVDD implementation. </LI> <LI> The experimental results demonstrate that the proposed classification strategy is feasible in recognizing the type of seabed sediments. </LI> </UL> </P>

Improved Mutual MRAS Speed Identification Based on Back-EMF

Hong Zheng,Jiancheng Zhao,Liangzhong Liu 대한전기학회 2016 Journal of Electrical Engineering & Technology Vol.11 No.3

In the design of sensorless control system for induction motor, high-precision speed estimation is one of the most difficult problems. To solve this problem, the common method is model reference adaptive method (MRAS). MRAS requires accurate motor parameters to estimate rotor speed precisely. However, when motor is running, the variety of temperature and magnetic saturation will lead to the change of motor parameters such as stator resistance and rotor resistance, which will lower the accuracy of the speed estimation. To improve the accuracy and rapidity of speed estimation, this paper analyses the mutual MRAS speed identification based on rotor flux linkage, and proposes an improved mutual MRAS speed identification based on back-EMF. The improved method is verified by Simulink simulation and motor experimental platform based on DSP2812. The results of simulation and experiment indicate that the method proposed by this paper can significantly improve the accuracy of speed identification, and speed up the response of identification.

Improved Mutual MRAS Speed Identification Based on Back-EMF

Zheng, Hong,Zhao, Jiancheng,Liu, Liangzhong The Korean Institute of Electrical Engineers 2016 Journal of Electrical Engineering & Technology Vol.11 No.3

In the design of sensorless control system for induction motor, high-precision speed estimation is one of the most difficult problems. To solve this problem, the common method is model reference adaptive method (MRAS). MRAS requires accurate motor parameters to estimate rotor speed precisely. However, when motor is running, the variety of temperature and magnetic saturation will lead to the change of motor parameters such as stator resistance and rotor resistance, which will lower the accuracy of the speed estimation. To improve the accuracy and rapidity of speed estimation, this paper analyses the mutual MRAS speed identification based on rotor flux linkage, and proposes an improved mutual MRAS speed identification based on back-EMF. The improved method is verified by Simulink simulation and motor experimental platform based on DSP2812. The results of simulation and experiment indicate that the method proposed by this paper can significantly improve the accuracy of speed identification, and speed up the response of identification.

Long-term continuously monocropped peanut significantly disturbed the balance of soil fungal communities

Chen Mingna,Zhang Jiancheng,Liu Hu,Wang Mian,Pan LiJuan,Chen Na,Wang Tong,Jing Yu,Chi Xiaoyuan,Du Binghai 한국미생물학회 2020 The journal of microbiology Vol.58 No.7

Balancing soil microbial diversity and abundance is critical to sustaining soil health, and understanding the dynamics of soil microbes in a monocropping system can help determine how continuous monocropping practices induce soil sickness mediated by microorganisms. This study used previously constructed gradient continuous monocropping plots and four varieties with different monocropping responses were investigated. The feedback responses of their soil fungal communities to short-term and long-term continuous monocropping were tracked using high-throughput sequencing techniques. The analyses indicated that soil samples from 1 and 2 year monocropped plots were grouped into one class, and samples from the 11 and 12 year plots were grouped into another, regardless of variety. At the species level, the F. solani, Fusarium oxysporum, Neocosmospora striata, Acrophialophora levis, Aspergillus niger, Aspergillus corrugatus, Thielavia hyrcaniae, Emericellopsis minima, and Scedosporium aurantiacum taxa showed significantly increased abundances in the long-term monocropping libraries compared to the short-term cropping libraries. In contrast, Talaromyces flavus, Talaromyces purpureogenus, Mortierella alpina, Paranamyces uniporus, and Volutella citrinella decreased in the long-term monocropping libraries compared to the shortterm libraries. This study, combined with our previous study, showed that fungal community structure was significantly affected by the length of the monocropping period, but peanut variety and growth stages were less important. The increase in pathogen abundances and the decrease in beneficial fungi abundances seem to be the main cause for the yield decline and poor growth of long-term monocultured peanut. Simplification of fungal community diversity could also contribute to peanut soil sickness under long-term monocropping. Additionally, the different responses of peanut varieties to monocropping may be related to variations in their microbial community structure.