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Zhang, Yuandong,Guo, Mingming,Wang, Xiaochun,Gu, Fengxue,Liu, Shirong Springer Japan 2018 Ecological research Vol.33 No.2
<P><B>Abstract</B></P><P>An anomalous reduction in tree growth indices has been detected in tree ring records from many circumpolar northern latitude sites in recent years, which was known as the divergence phenomenon. In order to understand whether the divergence phenomenon appeared at altitudinal treelines in east edge of Tibetan Plateau, we analyzed the correlation coefficients between tree growth and climate variables before and after recent climate warming, respectively. The results showed that divergence phenomenon took place and varied with sites. Compared with those in 1955–1982, the correlation coefficient of growth to mean temperature in June declined during 1983–2012. In Songpan site, more than half of the sampled trees showed an increasing positive correlation with temperature from July to September, while others turned to be a negative correlation. The positive responses to the mean temperature in previous‐year November and December declined in Markang site. Only in Miyaluo site, the positive correlation between tree ring index and temperature from July to September increased. Both the anomalous reduction or disappearance of the responses to the mean temperature and the divergent growth responses suggested that the divergence phenomenon was universal at altitudinal treelines in east edge of Tibetan Plateau, which had potentially significant influences on the estimation of forest productivity and climatic reconstructions based on tree ring.</P>
Wenjie Li,Huijun Guo,Yongbin Wang,Yongdun Xie,Linshu Zhao,Jiayu Gu,Shirong Zhao,Baocun Zhao,Guangjin Wang,Luxiang Liu 한국유전학회 2017 Genes & Genomics Vol.39 No.4
To identify novel allelic variations in key genes of wheat quality, the present study used the targeting induced local lesions in genomes platform to detect point mutations in target genes. The wheat variety Longfumai 17 was treated by the mutagen ethyl methanesulfonate to produce a bulk M2 generation, and the population included 1122 plants. A total length of 3906.80 kb nucleotides was analyzed, and the average mutation density was 1/244.17 kb. The identified mutations included G>A substitutions (43.75%), C>T substitutions (31.25%), A insertions (12.50%), T insertions (6.25%), and deletions (6.25%). These point mutations led to changes in amino acids and thus the encoded protein sequences, ultimately producing 18.75% of missense mutations, 12.50% of frame shift mutations, 6.25% of nonsense mutations, 25.00% of silent mutations and 37.50% of non-coding region mutations. In the kernel hardness gene Pinb and 3 starch synthesis genes waxy, Agp2 and SSIIa-A, we detected 16 different point mutations in 25 mutant lines. The Pinb gene harbored two missense mutations and a nonsense mutation; the C>T missense mutation resulted in a novel allele, this novel allele and the nonsense mutation alerted protein 3D structure; the waxy gene presented missense and frame shift mutations; the Agp2 gene carried a missense mutation; the SSIIa-A incurred a missense mutation and a frame shift mutation that resulted in premature protein termination. All the frame shift mutations, nonsense mutations and the Pinb novel allele resulted in allelic variation of their corresponding genes, which in turn affected their gene functions. The identified mutant lines can be used as intermediate materials in wheat quality improvement schemes.
Suppressive Effects of Coumarins on Pumpkin Seedling Growth and Glutathione S-Transferase Activity
Hossain, Md. Daud,Li, Jing,Guo, Shirong,Fujita, Masayuki The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.3
The effects of some coumarins(coumarin, 7-hydroxycoumarin, scopoletin and esculetin) were investigated on pumpkin(Cucurbita maxima Duch.) seedlings and on pumpkin glutathione S-transferases(GSTs). Coumarin and esculetin suppressed the growth of seedlings, especially the elongation of roots as well as hypocotyls. Among the compounds tested, only esculetin inhibited the activity of a particular pumpkin GST by 50%, CmGSTU3 toward 1-chloro-2, 4- dinitrobenzene(CDNB) and at a concentration of 22 ${\mu}M$. Both ethylacetae(EtOAc) and water fractions in pumpkin seedlings and different organs of one-month-old pumpkin plants contained esculetin or similar hydrophobic fluorescent substances as well as hydrophilic substances, which showed different degrees of inhibitory effects on CmGSTU3 activity.
Suppressive Effects of Coumarins on Pumpkin Seedling Growth and Glutathione S-Transferase Activity
Md. Daud Hossain,Jing Li,Shirong Guo,Masayuki Fujita 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.3
The effects of some coumarins (coumarin, 7-hydroxycoumarin, scopoletin and esculetin) were investigated on pumpkin (Cucurbita maxima Duch.) seedlings and on pumpkin glutathione S-transferases (GSTs). Coumarin and esculetin suppressed the growth of seedlings, especially the elongation of roots as well as hypocotyls. Among the compounds tested, only esculetin inhibited the activity of a particular pumpkin GST by 50%, CmGSTU3 toward 1-chloro-2, 4- dinitrobenzene (CDNB) and at a concentration of 22 μM. Both ethylacetae (EtOAc) and water fractions in pumpkin seedlings and different organs of one-month-old pumpkin plants contained esculetin or similar hydrophobic fluorescent substances as well as hydrophilic substances, which showed different degrees of inhibitory effects on CmGSTU3 activity. The effects of some coumarins (coumarin, 7-hydroxycoumarin, scopoletin and esculetin) were investigated on pumpkin (Cucurbita maxima Duch.) seedlings and on pumpkin glutathione S-transferases (GSTs). Coumarin and esculetin suppressed the growth of seedlings, especially the elongation of roots as well as hypocotyls. Among the compounds tested, only esculetin inhibited the activity of a particular pumpkin GST by 50%, CmGSTU3 toward 1-chloro-2, 4- dinitrobenzene (CDNB) and at a concentration of 22 μM. Both ethylacetae (EtOAc) and water fractions in pumpkin seedlings and different organs of one-month-old pumpkin plants contained esculetin or similar hydrophobic fluorescent substances as well as hydrophilic substances, which showed different degrees of inhibitory effects on CmGSTU3 activity.