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RAPD Variation and Genetic Distances among Tibetan, Inner Mongolia and Liaoning Cashmere Goats
Chen, Shilin,Li, Menghua,Li, Yongjun,Zhao, Shuhong,Yu, Chuanzhou,Yu, Mei,Fan, Bin,Li, Kui Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.11
Relationship among Tibetan cashmere goats, Inner Mongolia cashmere goats and Liaoning cashmere goats was studied using the technique of random amplified polymorphic DNA (RAPD). One primer and four primer combinations were screened. With the five primers and primer combinations, DNA fragments were amplified from the three breeds. Each breed has 28 samples. According to their RAPD fingerprint maps, the Nei's (1972) standard genetic distance was: 0.0876 between Tibetan cashmere goats and Inner Mongolia cashmere goats, 0.1601 between Tibetan cashmere goats and Liaoning cashmere goats, 0.0803 between the Inner Mongolia cashmere goats and Liaoning cashmere goats. It coincides with their geographic location. The genetic heterogeneity of Tibetan cashmere goats, Inner Mongolia cashmere goats and Liaoning cashmere goats is 0.3266, 0.2622 and 0.2475 respectively. It is also consistent with their development history.
3D Unsteady Simulation of the Transport Characteristics in the LEC Melt of In-Doped GaAs
Shuxian Chen,Shilin Li,Xiaoming Tan 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.5
A three-dimensional and time-dependent turbulent mathematical model is established for the mass, heat, momentum and dopant transport in the LEC melt of In-doped GaAs. The Solution scheme for the dopant segregation effect at the melt/crystal interface is put forward. Grid testing calculations have been performed for the choice of the grid. The turbulent mathematical model and numerical methodology are used to simulate the melt convections and dopant transports in the previously published experiments, and the numerical accuracy is validated by comparing the results with the experimental data in different model setups. Some transport characteristics in the LEC melt of In-doped GaAs have been concluded. Owing to the interacting forces associated with different length scales in the LEC melt, the fluid flow is non-axisymmetric. Because of the competition between buoyancy, Marangoni force, centrifugal force and the Coriolis force, the temperature fluctuates in the melt. Due to the segregation, the InAs concentration increase in the axial direction.
Chenguang Zhu,Shilin Chen,Guimin Zhang,Wei Wang,Yuanping Tang,Bing Mei,Rentao Song 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.4
The overexpression of algal glutamine synthetase (GS) gene DvGS2 in Arabidopsis thaliana resulted in higher plant biomass and better growth phenotype. The purpose of this study was to understand the biological mechanism for the improvement of growth phenotype of DvGS2-transgenic Arabidopsis. A series of molecular and biochemical investigation related to nitrogen and carbon metabolism in DvGS2-transgenic line was, respectively, conducted. Analysis of nitrogen use efficiency (NUE)-related gene expression and enzymatic activity revealed that the transcriptional level and enzymatic activity of genes encoding glutamate synthase, glutamate dehydrogenase and alanine aminotransferase were significantly up-regulated especially under low nitrate condition. The DvGS2- transgenic line showed higher total nitrogen content and lower carbon: nitrogen ratio than the wild type. Significant reduced concentration of leaf free ammonium, higher concentrations of total amino acids, individual amino acid (glutamine, methionine, alanine, asparagine) together with increased concentrations of glucose, fructose and starch, confirmed the higher efficiency of nitrogen and carbon assimilation. These improved metabolisms of nitrogen and carbon conferred the DvGS2-transgenic Arabidopsis more biomass, better growth phenotype and higher NUE compared with the wild type plant.
Efficient removal of Cr(VI) by spent coffee grounds: Molecular adsorption and reduction mechanism
Yue Hu,Meiting Zhi,Shilin Chen,Wenguan Lu,Yinlong Lai,Xiaobing Wang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.7
Spent coffee ground (SCG), a byproduct from the soluble coffee industry, is usually discarded as waste. Thereutilization of SCG for the removal of toxic heavy metal ions is a novel research direction. Until recently, the molecularadsorption and reduction mechanism of Cr(VI) on SCG was barely investigated. In this study, SCG was used forthe efficient removal of Cr(VI) at a concentration range of 2-100mg/L, with a maximum Cr(VI) uptake up to 36.2mg/g. Structural characterization and ATR-FTIR analysis indicated that SCG possessed abundant surface O and N- containingfunctional groups. The corresponding adsorption and reduction effects on the Cr(VI) removal were investigatedby the carboxyl and hydroxyl groups elimination experiments and ATR-FTIR characterization, respectively. Theresults revealed that HCrO4 ions were preliminarily adsorbed on SCG surfaced-COOH/-OH/-NH by the formation ofhydrogen bond (SCG surfaced-COOH/-OH/-NH···HCrO4), and quickly reduced to Cr(III) by the electron denoted byphenolic compounds, and then in-situ immobilized on the surface of SCG. The effect of Cr(VI) concentration, coexistingions, and humic acid was systematically studied to optimize the removal of Cr(VI) wastewater. Column experimentsprovided a new substitution to restore the Cr(VI)-containing groundwater for the permeable reactive barrierapplication. Thus, the proposed study uncovered the intrinsic Cr(VI) removal mechanism at the molecular level andexplored the application of SCG for the efficient removal of Cr(VI).
( Zi-jiang Li ),( Shilin Chen ),( Xiang-hao Yang ),( Rui Wang ),( Hee-jeong Min ),( Lei Wu ),( Chuan-ling Si ),( Young-soo Bae ) 한국목재공학회 2018 목재공학 Vol.46 No.2
Juglans mandshurica is a fast growing hard species, which is a tree in family of Juglandaceae and has a wide distribution in China, Korea and eastern Russia. Plant materials from J. mandshurica have extensively been used in folk medicines to prevent or cure gastric, esophageal, lung and cardiac cancer. As one chain of our searching for anticomplementary agents from natural sources, two epimeric ellagitannins, [2,3-O-4,4′,5,5′,6,6′,-hexahydroxydiphenoyl (HHDP))-(α,β)-D-glucose] (I) and pedunculagin (II) were purified from 70% acetone extracts of the stem barks of J. mandshurica by Thin Layer Chromatography and Sephadex LH-20 column chromatography approaches. The chemical structures of the isolated compounds were characterized by MS, NMR, and a careful comparation with published literatures. The epimeric ellagitannins I and II exhibited inhibitory properties against a classical pathway of complementary system with 50 % inhibitory concentrations (IC<sub>50</sub>) values of 65.3 and 47.7 μM, respectively, comparing with riliroside (IC<sub>50</sub>=104 μM) and rosmarinic acid (IC<sub>50</sub>=182 μM), which were used as positive controls. Thus, the work indicated both the two secondary metabolites possess excellent inhibitory activity and might be developed as potential anticomplementary chemicals.
Wei, Guangfei,Yang, Feng,Wei, Fugang,Zhang, Lianjuan,Gao, Ying,Qian, Jun,Chen, Zhongjian,Jia, Zhengwei,Wang, Yong,Su, He,Dong, Linlin,Xu, Jiang,Chen, Shilin The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.6
Background: Panax quinquefolius and Panax notoginseng are widely used and well known for their pharmacological effects. As main pharmacological components, saponins have different distribution patterns in the root tissues of Panax plants. Methods: In this study, the representative ginsenosides were detected and quantified by desorption electrospray ionization mass spectrometry and high-performance liquid chromatography analysis to demonstrate saponin distribution in the root tissues of P. quinquefolius and P. notoginseng, and saponin metabolite profiles were analyzed by metabolomes to obtain the biomarkers of different root tissues. Finally, the transcriptome analysis was performed to demonstrate the molecular mechanisms of saponin distribution by gene profiles. Results: There was saponin distribution in the root tissues differed between P. quinquefolius and P. notoginseng. Eight-eight and 24 potential biomarkers were detected by metabolome analysis, and a total of 340 and 122 transcripts involved in saponin synthesis that were positively correlated with the saponin contents (R > 0.6, P < 0.05) in the root tissues of P. quinquefolius and P. notoginseng, respectively. Among them, GDPS1, CYP51, CYP64, and UGT11 were significantly correlated with the contents of Rg1, Re, Rc, Rb2, and Rd in P. quinquefolius. UGT255 was markedly related to the content of R1; CYP74, CYP89, CYP100, CYP103, CYP109, and UGT190 were markedly correlated with the Rd content in P. notoginseng.
Xu Yuxuan,Tu Chuanjun,Liu Yanli,Liu Ping,Chen Gang,Tan Jiao,Xiong Wei,He Yubo,Liang Shilin,Ma Qingchun 한국탄소학회 2022 Carbon Letters Vol.32 No.5
High-temperature friction performances of graphite blocks (GBs) and zinc phosphate impregnated graphite blocks (IGBs) were evaluated under various friction temperatures. The surface of IGB exhibited extremely lower average friction coefficient values, that was 0.007 at 400 °C and 0.008 at 450 °C, in comparison to that of GB (0.13 at 400 °C and 0.16 at 450 °C, respectively). The worn surface of IGB in the high-temperature friction test was smoother and more complete than that of GB. The wear under high temperature and load caused the transformation of zinc pyrophosphate to zinc metaphosphate and the formation of a continuous large-area boundary lubrication layer combined with graphite and metallic element on the wear surface. The superior tribology property of IGB could be attributed to the digestion of iron oxides by tribo-chemical reactions and passivation of the exposed dangling covalent bonds. Specifically, the layered structure generated on the IGB wear interface effectively decreased the adhesive forces and prevented the surface from serious damage.