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      • KCI등재

        Composition and diversity of ground-dwelling arthropods at Chiayi Agricultural long-term ecological research site in the Southern Taiwan

        Sheng-Shan Lu,Shou-Horng Huang,Laleh Bordbar,I-Hsin Sung 국립중앙과학관 2019 Journal of Asia-Pacific Biodiversity Vol.12 No.4

        In this study, the canola oil and alcohol were used as attractants in pitfall traps to help collect grounddwellingarthropods. Two small agricultural long-term ecological research fields at Chiayi AgriculturalExperimental Station were chosen to execute sampling activities. There were 14,509 individuals of 182species (including morphospecies) in 18 taxa in the collections. The most abundant taxon was Hymenoptera,followed by Coleoptera, Collembola, Diptera, Orthoptera, and Acari. Regardless to the differenceof the sampling fields, canola oil trap was significantly attractive to ants (Formicidae, Hymenoptera). Onthe other hand, the alcohol trap was more effective in attracting the Coleoptera. According to the diversityanalysis, the results indicated that the diversity indexes were affected by the main effect forattractant. In addition, the interactions between the farming operation and samplings and between theattractant and samplings were also factors to influence the diversity of ground-dwelling arthropods inthe long-term ecological research site of Chiayi Station.

      • KCI등재

        Dynamic mechanism of HIV replication inhibitor peptide encapsulated into carbon nanotubes

        Bao-Dong Chen,Chuan-Lu Yang,Jun-Sheng Yang,Mei-Shan Wang,Xiao-Guang Ma 한국물리학회 2013 Current Applied Physics Vol.13 No.6

        Biomolecules encapsulated in carbon nanotubes (CNTs) have attracted much interest and facilitated exciting opportunities for biological and biomedical applications of CNTs. Understanding the fundamental interaction and change in biomolecules during encapsulation is indispensable but remains a challenge for both theoretical and experimental investigations. This paper focuses on the interaction between HIV replication inhibitor peptide (HRIP) and CNTs in a neutral solution with molecular dynamics simulation. We observed that HRIP spontaneously inserts into the CNTs and oscillates around the center of the tube, where the non-covalent interaction is minimum. The effects of the diameters of the CNTs on HRIP were investigated. The optimal diameter of the CNT that can provide the most effective encapsulation and causes minimum conformational change in HRIP was found. The present results provide valuable insights in the understanding of nanoscale drug delivery using CNT-based devices.

      • Platycodin D Induces Apoptosis, and Inhibits Adhesion, Migration and Invasion in HepG2 Hepatocellular Carcinoma Cells

        Li, Ting,Xu, Wen-Shan,Wu, Guo-Sheng,Chen, Xiu-Ping,Wang, Yi-Tao,Lu, Jin-Jian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

        Background: Platycodin D (PD), a triterpenoid saponin isolated from the Chinese medicinal herb Platycodonis radix, possesses anti-cancer effects in several cancer cell lines. The aim of this study was to evaluate its anticancer activities in hepatocellular carcinoma cells. Materials and Methods: MTT and colony formation assays were performed to evaluate cell proliferation, along with flow cytometry and Western blotting for apoptosis. Cell adhesion was tested by observing cellular morphology under a microscope, while the transwell assay was employed to investigate the cell migration and invasion. Results: PD concentration-dependently inhibited cell proliferation in both HepG2 and Hep3B cells, and significantly suppressed colony formation and induced apoptosis in HepG2 cells. The protein levels of cleaved poly ADP-ribose polymerase (PARP) and Bax were up-regulated while that of survivin was down-regulated after treatment with PD. Moreover, PD not only obviously suppressed the adhesion of HepG2 cells to Matrigel, but also remarkably depressed their migration and invasion induced by 12-O-tetradecanoylphorbol 13-acetate (TPA). Conclusions: PD presents anti-cancer potential in hepatocellular carcinoma cells via inducing apoptosis, and inhibiting cell adhesion, migration and invasion, indicating promising features as a lead compound for anti-cancer agent development.

      • KCI등재

        Reusing indigenous plant materials to restore vegetation: A practical case in Shei-Pa National Park

        Ching-An Chiu,Sheng-Shan Lu,Mei-Fen Lee,Chen-Chang Pan,Kuo-Min Fu 국립중앙과학관 2020 Journal of Asia-Pacific Biodiversity Vol.13 No.2

        Using only local gene pools to restore native vegetation is challenging. This practice enabled native forestin a post-fired pine and silvergrass woodland to be rehabilitated by reusing indigenous materials in asimple and effective manner. We designed vegetation restoration processes, including uprooting silvergrassand removing pine leaf from woodland ground, collecting tree saplings on sliding slopes andtransplanting them, and collecting humus and seeds from roadsides and redistributing them at restorationsites. Volunteers, tourists, and employees of Shei-Pa National Park participated in the restorationpractices. Through 5-year practices, the native vegetation and forest floor environment exhibited aremarkable recovery, inducing the reappearance of the Guanwu salamander. This restoration practicecost little money but required much time and labor. The approach may be suitable for small restorationsites. We believe that this practice could enhance knowledge, technology, and enthusiasm for localecological restoration.

      • KCI등재

        Correlation between Intravoxel Incoherent Motion Magnetic Resonance Imaging Derived Metrics and Serum Soluble CD40 Ligand Level in an Embolic Canine Stroke Model

        Xiao-Quan Xu,Chen-Jiang Wu,Shan-Shan Lu,Qian-Qian Gao,Qing-Quan Zu,Xing-Long Liu,Hai-Bin Shi,Sheng Liu 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.5

        Objective: To determine the relationship between intravoxel incoherent motion (IVIM) imaging derived quantitative metrics and serum soluble CD40 ligand (sCD40L) level in an embolic canine stroke model. Materials and Methods: A middle cerebral artery occlusion model was established in 24 beagle dogs. Experimental dogs were divided into low- and high-sCD40L group according to serum sCD40L level at 4.5 hours after establishing the model. IVIM imaging was scanned at 4.5 hours after model establishment using 10 b values ranging from 0 to 900 s/mm2. Quantitative metrics diffusion coefficient (D), pseudodiffusion coefficient (D*), and perfusion fraction (f) of ischemic lesions were calculated. Quantitative metrics of ischemic lesions were normalized by contralateral hemisphere using the following formula: normalized D = Dstroke / Dcontralateral. Differences in IVIM metrics between the low- and high-sCD40L groups were compared using t test. Pearson’s correlation analyses were performed to determine the relationship between IVIM metrics and serum sCD40L level. Results: The high-sCD40L group showed significantly lower f and normalized f values than the low-sCD40L group (f, p < 0.001; normalized f, p < 0.001). There was no significant difference in D*, normalized D*, D, or normalized D value between the two groups (All p > 0.05). Both f and normalized f values were negatively correlated with serum sCD40L level (f, r = -0.789, p < 0.001; normalized f, r = -0.823, p < 0.001). However, serum sCD40L level had no significant correlation with D*, normalized D*, D, or normalized D (All p > 0.05). Conclusion: The f value derived from IVIM imaging was negatively correlated with serum sCD40L level. f value might serve as a potential imaging biomarker to assess the formation of microvascular thrombosis in hyperacute period of ischemic stroke.

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