http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Shen, Chaohui,Gao, Xiyang,Li, Tao,Zhang, Jun,Gao, Yuqian,Qiu, Liyou,Zhang, Guang The Korean Society of Mycology 2019 Mycobiology Vol.47 No.3
The $11{\alpha}$-hydroxylation of $16{\alpha}$, 17-epoxyprogesterone (EP) catalyzed by Rhizopus nigricans is crucial for the steroid industry. However, lower conversion rate of the biohydroxylation restricts its potential industrial application. The $11{\alpha}$-steroid hydroxylase CYP509C12 from R. oryzae were reported to play a crucial role in the $11{\alpha}$-hydroxylation in recombinant fission yeast. In the present study, the CYP509C12 of R. oryzae (RoCYP) was introduced into R. nigricans using the liposome-mediated mycelial transformation. Heterologous expression of RoCYP resulted in increased fungal growth and improved intracellular reactive oxygen species content in R. nigricans. The $H_2O_2$ levels in RoCYP transformants were approximately 2-folder that of the R. nigricans wild type (RnWT) strain, with the superoxide dismutase activities increased approximately 45% and catalase activities decreased approximately 68%. Furthermore, the $11{\alpha}$-hydroxylation rates of EP in RoCYP transformants (C4, C6 and C9) were 39.7%, 38.3% and 38.7%, which were 12.1%, 8.2% and 9.4% higher than the rate of the RnWT strain, respectively. This paper investigated the effect of heterologous expression of RoCYP in R. nigricans, providing an effective genetic method to construct the engineered strains for steroid industry.
( Chaohui Shen ),( Xiyang Gao ),( Tao Li ),( Jun Zhang ),( Yuqian Gao ),( Liyou Qiu ),( Guang Zhang ) 한국균학회 2019 Mycobiology Vol.47 No.3
The 11a-hydroxylation of 16a, 17-epoxyprogesterone (EP) catalyzed by Rhizopus nigricans is crucial for the steroid industry. However, lower conversion rate of the biohydroxylation restricts its potential industrial application. The 11a-steroid hydroxylase CYP509C12 from R. oryzae were reported to play a crucial role in the 11a-hydroxylation in recombinant fission yeast. In the present study, the CYP509C12 of R. oryzae (RoCYP) was introduced into R. nigricans using the liposome-mediated mycelial transformation. Heterologous expression of RoCYP resulted in increased fungal growth and improved intracellular reactive oxygen species content in R. nigricans. The H<sub>2</sub>O<sub>2</sub> levels in RoCYP transformants were approximately 2-folder that of the R. nigricans wild type (RnWT) strain, with the superoxide dismutase activities increased approximately 45% and catalase activities decreased approximately 68%. Furthermore, the 11a-hydroxylation rates of EP in RoCYP transformants (C4, C6 and C9) were 39.7%, 38.3% and 38.7%, which were 12.1%, 8.2% and 9.4% higher than the rate of the RnWT strain, respectively. This paper investigated the effect of heterologous expression of RoCYP in R. nigricans, providing an effective genetic method to construct the engineered strains for steroid industry.
高申(Shen Gao),蒲川(Chuan Pu),王宝秦(Baoqin Wang),蒋祎(Yi Jiang) 아시아사회과학학회 2023 International Science Research Vol.3 No.3
元认知是对认知的认知,其与案例教学结合的优势体现在学生通过重新认知案例教学过程和学习行为,可以强化案例教学的效果。本研究是在研究生《医疗卫生法律法规》课程中开展基于元认知的案例教学以期对课程思政的教学方式进行创新。基于元认知的案例教学实践分为四个部分:制定教学目标、形成课程思政案例微课库、开展案例教学和教学评价。学生评价结果表明,基于元认知理论进行课程思政案例教学能显著提升学习效果,计划、监控和调节是提升元认知能力的三大策略。 Metacognition is the cognition of cognition, and the advantage of its combination with case teaching is reflected in that students can strengthen the effect of case teaching by re-recognizing the process and learning behavior of case teaching. This study is to carry out metacognition-based case teaching in the course of Medical Health Laws and Regulations for postgraduate students in order to innovate the teaching methods of ideological and political education. The practice of case teaching based on metacognition is divided into four parts: formulating teaching objectives, forming the micro-class library of curriculum ideological and political cases, carrying out case teaching and teaching evaluation. The evaluation results of students show that the course case teaching based on metacognitive theory can significantly improve the learning effect, and planning, monitoring and adjustment are the three strategies to improve the metacognitive ability.
Aldehyde Volatiles Emitted in Succession from Mechanically Damaged Leaves of Poplar Cuttings
( Zeng Hui Hu ),( Ying Bai Shen ),( You Qing Luo ),( Fan Yi Shen ),( Hai Bo Gao ),( Rong Fu Gao ) 한국식물학회 2008 Journal of Plant Biology Vol.51 No.4
Plant aldehydes are volatiles necessary to defenses against environmental stress. To explore their emissions in response to wounding, we performed gas chromatography-mass spectrometry (GC-MS) on cuttings from poplar (Populus simonii×P. pyramidalis ``Opera 8277``) that were mechanically damaged to mimic herbivore attack. We detected 16 aldehydes, including 11 linear saturated aldehydes, 3 linear unsaturated aldehydes, and 2 non-linear aldehydes. Emissions of these aldehydes were clearly enhanced by such treatment, and exhibited a similar pattern of change, i.e., increasing in the first 2 h, then sharply decreasing before rising again at about 12 h. Two release peaks for these aldehydes were observed. Therefore, we propose two pathways for the mediation of aldehyde emissions following damage. The first peak may represent emissions from plant storage pools, whereas the second release peak might result from greater formation de novo through an activated synthesis pathway.
Influence of wheat-walnut intercropping on the Sitobion avenae and its predatory natural enemies
Gao Haifeng,Jia Shuangshuang,Liu Yongqiang,Shen Yuyang,Li Guangkuo,Zhao Sifeng,Ge Feng 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1
Intercropping is widely applied all over the world, and it often causes significant changes in the number of arthropod communities. Walnut-wheat intercropping systems are commonly applied in Southern Xinjiang of China. But it is still not clear how wheat-walnut intercropping influence pest and natural enemy populations, and their ratio in wheat fields. To clarify this question and provide guidance to pest management, field surveys were conducted in Xinjiang during 2017–2019. Three intercropping treatments were tested, which were wheat-walnut 10:6, 25:6, and monoculture wheat. Our results showed that the abundance of predators (mainly ladybeetles and lacewings) increased in wheat under wheat-walnut intercropping systems (especially in intercrop treatment 10:6). Compared with monoculture wheat, the ratio of predators to Sitobion avenae was not enhanced in wheatwalnut intercropping fields. S. avenae populations increased in the walnut intercropped wheat fields. The intercropping system was not beneficial to S. avenae control in wheat fields. Different measures should be taken to strengthen the control of S. avenae in walnut intercropped wheat fields.
Shen Yuan,Duan Zhengang,Lu Chuan,Ji Li,Jiao Caishan,Hou Hongguo,Chao Nan,Zhang Meng,Zhou Yu,Gao Yang 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.12
The porous corrosion deposits (known as CRUD) adhered to the cladding have an important effect on the heat transfer from fuel rods to coolant in PWRs. The vapor film is the main constituent in the two-phase film boiling model. This paper presents a vapor film thickness correlation, associated with CRUD porosity, CRUD chimney density, CRUD particle size, CRUD thickness and heat flux. The dependences of the vapor film thickness on the various influential factors can be intuitively reflected from this vapor film thickness correlation. The temperature, pressure, and boric acid concentration distributions in CRUD can be well predicted using the two-phase film boiling model coupled with the vapor film thickness correlation. It suggests that the vapor thickness correlation can estimate the vapor film thickness more conveniently than the previously reported vapor thickness calculation methods.
Shen Qiaoqiao,Luo Xiangping,Feng Xiangang,Gao Yulin 한국간호과학회 2022 Asian Nursing Research Vol.16 No.3
Purpose: The aim of this paper is to develop a scale for measuring the perinatal bereavement care competence of midwives and assess its psychometric properties. Methods: The Perinatal Bereavement Care Competence Scale was developed in four phases. (1) Item generation: 75 items were formulated based on a literature review and interviews with midwives. (2) Delphi expert consultation: 15 experts evaluated whether the items were clear/appropriate/relevant to the questionnaire dimensions, and the items were optimized. (3) Pilot test: The comprehensibility, acceptability, and time required to complete the questionnaire by midwives were assessed. (4) Evaluation of reliability and validity: The scale was validated by initial item analysis, exploratory and confirmatory factor analyses, and internal consistency reliability and testeretest reliability. Results: The final scale consisted of six dimensions and 25 items: maintaining belief (three items), knowing (four items), being with (six items), preserving dignity (four items), enabling (five items), and self-adjustment (three items). Exploratory factor analysis yielded a six-factor structure that was consistent with the theoretical framework and explained 70.8% of the total variance. Confirmatory factor analysis indicated a good fit for the six-factor model. Cronbach's a for the scale was 0.931, and the test eretest reliability coefficient was 0.968. Conclusion: The Perinatal Bereavement Care Competence Scale is a valid and reliable instrument for measuring the competence of midwives in caring for bereaved parents who have experienced perinatal loss.
Shen Guanghai,Kiem Phan Van,Cai Xing-Fu,Li Gao,Dat Nguyen Tien,Choi Yeon A,Lee Young Mi,Park Yong Ki,Kim Young Ho The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.6
A new biflavonol glycoside named as solanoflavone (1) was isolated from aerial part of Solanum melongena. The chemical structure was elucidated as isorhamnetin-3-O-$\beta$-D-glucopyranoside-(4'$\to$O$\to$4"')-galangin-3"-O-$\to$-D-glucopyranoside on the basis of physicochemical and spectroscopic methods, including 2D NMR spectral techniques.