Expression of Connexin 43 and E-cadherin Protein and mRNA in Non-small Cell Lung Cancers in Chinese Patients

Zhao, Jun-Qiang,Sun, Fang-Jie,Liu, Shan-Shan,Yang, Jun,Wu, Yu-Quan,Li, Gui-Shan,Chen, Qing-Yong,Wang, Jia-Xiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Aim: Connexin 43 (Cx43) and E-cadherin are important biomarkers related with cancer. Their expression at protein and mRNA levels was here investigated in 50 primary lung carcinoma tissues and 20 samples of adjacent normal tissue of Chinese patients with non-small cell lung cancer (NSCLC). Methods: Protein and mRNA expression were evaluated by ABC immunohistochemistry and RT-PCR. Results: (1) The positive expression rates of Cx43 and E-cadherin protein were higher in the adjacent normal tissues than those in the primary lung carcinoma tissues; (2) the positive expression rates of Cx43 and E-cadherin protein decreased with NSCLC progression; (3) the expression of E-cadherin protein was not related with the pathological type of NSCLC; and (4) the relative quantity of the Cx43 or E-cadherin mRNA expression was correlated with the the histological type, clinical stage, cancer cell differentiation and the lymph node metastasis. Conclusion: The data suggested that the Cx43 and E-cadherin are reduced with NSCLC progression, and might be important biomarkers for judging the metastasis and prognosis.

문화 교류 인식과 국가의 지도자 이미지가 국가브랜드에 미치는 영향

유산산 ( Shan Shan Liu ),전종우 ( Jong Woo Jun ) 전남대학교 사회과학연구소 2020 현대사회과학연구 Vol.24 No.-

본 연구는 외래문화를 도입할 때 자국민들이 받아들일 수 있는 정도, 한중 간 분쟁해결에 대한 인식, 중국 문화콘텐츠 이용 정도, 중국 국가지도자인 시진핑에 대한 인식과 태도를 살펴보고, 이러한 변인들이 한국인들의 중국에 대한 태도와 방문의도에 어떠한 영향을 미치는지 알아보고자 한다. 그에 따라 양국 간의 우호적인 관계 회복에 필요한 요소들을 제시하고자 한다. 연구를 위해 총8개의 독립변인을 선택하여 위계적 회귀분석을 통해 가설을 검증하였다. 독립변인으로는 다른 나라의 문화를 받아들이는 정도로서 문화유입 수용성과 국가 간의 분쟁문제해결에 대한 인식, 중국 콘텐츠 이용 정도를 선택하였다. 국가지도자 이미지 관련변인으로는 시진핑 공신력과 시진핑에 대한 태도를 활용하였으며, 추가적으로 기본적 인구통계학적 변인인 성별, 연령 등을 사용하였다. 수집된 자료는 SPSS 21.0을 사용하여 분석하였다. 문화유입 수용성과 중국 콘텐츠 이용, 그리고 시진핑에 대한 태도가 중국 국가태도에 긍정적인 영향을 미치는 것으로 나타났다. 또한 연령, 분쟁문제해결 인식, 중국 콘텐츠 이용, 시진핑에 대한 태도가 중국 방문의도에 정적인 영향을 미치는 것으로 검증되었다. 결과적으로 연구 내에서 타국의 문화와 지도자 이미지에 대한 인식은 해당 국가에 대한 태도와 방문 의도에 영향을 미치는 것으로 확인하였다. 본 연구는 국가브랜드를 형성하는 요소로 문화와 지도자의 역할을 제안함으로써 연구와 실무에 시사점을 제공하고자 한다. The study sought to explore the impact of Koreans' cultural awareness of China and Chinese national leader Xi Jinping's perception of China on attitudes and willingness to visit China. As a demographic variable, gender and age were used, and cultural inflow acceptance variables were utilized as a degree of acceptance of culture in other countries. In the event of trouble-fighting among countries, the perception of problem-solving was also chosen as one of the variables, and the level of use of Chinese content was prescribed in the amount and frequency of Korean people using Chinese cultural products. Finally, regarding the national leader's image, Xi Jinping's public confidence and attitude toward Xi Jinping were utilized. A total of eight independent variables have an impact on China's state attitude and willingness to visit China. Tests of hypotheses using hierarchical regression have shown that acceptance of culture, use of Chinese contents, and attitude toward Xi Jinping have positive effects on China's national attitude. As a result, the better the cultural perception of China and attitude toward Chinese leader Xi Jinping, the more positive China's attitude and willingness to visit the country is overall. It aims to provide implications for research and practice by proposing the role of culture and leader as elements that shape the national brand.

Patterns of Failure and Survival Trends in 3,808 Patients with Stage II Nasopharyngeal Carcinoma Diagnosed from 1990 to 2012: A Large-Scale Retrospective Cohort Study

Xue-Song Sun,Di-Han Liu,Sai-Lan Liu,Qiu-Yan Chen,Shan-Shan Guo,Yue-Feng Wen,Li-Ting Liu,Hao-Jun Xie,Qing-Nan Tang,Yu-Jing Liang,Xiao-Yun Li,Jin-Jie Yan,Ming-Huang Hong,Jun Ma,Lin-Quan Tang,Hai-Qiang M 대한암학회 2019 Cancer Research and Treatment Vol.51 No.4

Purpose The purpose of this study was to investigate the survival trends and patterns of failure in patients with stage II nasopharyngeal carcinoma (NPC) treated with radiotherapy (RT) and chemotherapy over the last 20 years. Materials and Methods Thirty-eight hundred and eight patients diagnosed with stage II NPC between January 1990 and December 2012 were involved in this retrospective cohort study. All patients were treated with RT. According to the main imaging techniques and RT technology, we categorized these patients into four calendar periods: 1990-1996, 1997-2002, 2003-2007, and 2008-2012. Overall survival (OS), progression-free survival (PFS), locoregional relapse-free survival (LRFS), and distant metastasis–free survival (DMFS) were served as the clinical outcome. Results After a median follow-up period of 84.7 months, we observed increasing trends in survival and disease control. The 3- and 5-year OS rates increased from 87.1% and 78.7% in the first calendar period to 97.4% and 94.5% in the last calendar period, respectively (p < 0.001). Additionally, significant increasing trends could be seen in the PFS and LRFS during the four calendar periods. In the subgroup analysis, the LRFS in patients older than 50 years at diagnosis showed greater improvement than younger patients. However, the rate of distant metastasis was stable and relatively low, as the 5-year DMFS ranged from 90.5% to 94.7% among the four calendar periods. Conclusion The survival rates in patients with stage II NPC showed increasing trends from 1990 to 2012. The advance of RT provided excellent locoregional control and enhanced OS.

Analysis of Mammographic Breast Density in a Group of Screening Chinese Women and Breast Cancer Patients

Liu, Jing,Liu, Pei-Fang,Li, Jun-Nan,Qing, Chun,Ji, Yu,Hao, Xi-Shan,Zhang, Xue-Ning Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: A dense breast not only reduces the sensitivity of mammography but also is a moderate independent risk factor for breast cancer. The percentage of Western women with fat breast tissue is higher aged 40 years or older. To a certain extent, mammography as a first choice of screening imaging method for Western women of this group is reasonable. Hitherto, the frequency and age distribution of mammographic breast density patterns among Chinese women had not been characterized. The purpose of this study was to investigate the frequency and age distribution of mammographic breast density patterns among a group of Chinese screening women and breast cancer patients in order to provide useful information for age-specific guidelines for breast cancer screening in Chinese women. Methods: A retrospective review of a total of 3,394 screening women between August and December 2009 and 2,527 breast cancer patients between July 2011 and June 2012 was conducted. Descriptive analyses were used to examine the association between age and breast density. The significance of differences of breast density between the screening women and the breast cancer patients was examined using nonparametric tests. Results: There was a significant inverse relationship between age and breast density overall (r=-0.37, p< 0.01). Breast density of the breast cancer patients in the subgroups of 40-49 years old was greater compared with that of the screening women, the same in those aged 50-54 years and in those 55 years old or older, less than in the screening group. Conclusions: With regard to the Chinese women younger than 55 years old, the diagnostic efficiency of breast cancer screening imaging examinations may be potentially improved by combining screening mammography with ultrasound.

Neuron Discharge and c-Fos Expression in the Nucleus of the Solitary Tract Following Electroacupuncture at Acupoints of the Yangming Stomach Meridian of Foot

Jun-Feng He,Jie Yan,Jiang-Shan Li,Jian-Hua Liu,Chao Wang,Xiao-Rong Chang,Ya-Ting Qu 사단법인약침학회 2013 Journal of Acupuncture & Meridian Studies Vol.6 No.2

The nucleus of the solitary tract (nucleus tractus solitarii; NTS) is a primary center for both visceral afferents and somatic afferents. Previous experiments have demonstrated that the NTS is closely connected to the stomach and acupoints in the Yangming Stomach Meridian of Foot (ST Meridian). In this study, extracellular recording and immunochemistry methods were used to analyze the discharge of neurons and c-Fos protein expression in the NTS following acupuncture at different acupoints and a nonacupoint. A total of 104 discharging neurons were detected in the NTS of 52 rats, of which 86 provided complete data. After acupuncture at Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint, the neuron response rate in the NTS was 65.12%, 51.16%, 46.51%, 34.88% and 31.40% respectively. For neuron response rate, there was a significant difference among Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint (p < 0.01 or p < 0.05). In the other 48 rats, the number of c-Fos immunoreactive neurons in the NTS by electroacupuncture (EA) at Sibai (ST 2) group was significantly higher than that EA at other acupoints and the nonacupoint (p < 0.05 or p < 0.01). EA at both Zusanli (ST 36) and Neiting (ST 44) increased c-Fos immunoreactive neurons significantly over EA at Quanliao (SI 18) and the nonacupoint (p < 0.05 or p < 0.01), while there was no difference between EA at Quanliao (SI 18) and the nonacupoint group (p > 0.05). The experiments demonstrated that the afferent convergence in NTS are different by body surface points stimulus, which suggests that the NTS might be a primary center in the central nervous system receiving acupoints stimulus from the ST Meridian.

ApCYP4C6 participates in the metabolism of glucosinolates in pea aphids Acyrthosiphon pisum

Liu Lei,Wang Wen-Jun,Song Li-Wen,Wu Yi-Ting,Wei Jiang-Wen,Wang Sen-Shan,Zhou Jing-Jiang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.1

Cytochrome P450 enzymes play a key role in the metabolism of plant allelochemicals of herbivorous insects. Here, we investigated the role of cytochrome P450 enzyme genes in the metabolism of glucosinolates in the pea aphids. The results showed that compared with the control untreated group, the transcription levels of two cytochrome P450 enzyme genes, ApCYP4C6 and ApCYP4C7, were significantly increased at 24 h and 48 h after glucosinolates treatment. Furthermore, after the aphids were treated with dsCYP4C6 (double stranded CYP4C6) for 48 h, the expression level of ApCYP4C6 in the aphids was significantly decreased, and its mortality was significantly increased by glucosinolates comparing with that of the dsGFP group. The present study demon strated that ApCYP4C6 was involved in the metabolism of glucosinolates by the pea aphids and contributed to further understanding the role of P450 genes in the tolerance of the pea aphids to plant secondary metabolites.

Analysis of non-point and point source pollution load in DongPieHong Ditch

Shan, Yu,Chen, Jun,Jin, Jie,Song, YongLian,Liu, Jun,Wu, DongBiao,Wu, Ke Urban Science Institute 2019 도시과학 Vol.8 No.2

In this study, the DongPieHong Ditch were taken as the research object, five sampling points were set to measure the COD, NH₃-N,TNandTPindexes. The correlation and principal component analysis were used to judge the main pollution sources and calculate pollution contribution rate. According to the population in the basin, the load of point source pollution into the river was estimated. As a result, the load of COD, NH₃-N and TP into the river was 323.04t/a, 43.8t/a and 3.9t/a, respectively. According to the statistics of the rainfall in the basin, the concentrations of COD, TP and NH₃-N in the initial rainwater were measured and calculated for non-point source pollution, and the results shown that the inflow loads of COD, NH₃-N and TP into the river were 34.59t/a, 0.12t/a and 0.71t/a, respectively. It was found that the main cause of the pollution in the east flash flood gully was point source pollution, and the proportions of COD, NH₃-N and TP into the river were 90.33%, 99.72% and 84.61%, respectively.

Time-domain analyses of the layered soil by the modified scaled boundary finite element method

Shan Lu,Jun Liu,Gao Lin,Wenyuan Wang 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.55 No.5

The dynamic response of two-dimensional unbounded domain on the rigid bedrock in the time domain is numerically obtained. It is realized by the modified scaled boundary finite element method (SBFEM) in which the original scaling center is replaced by a scaling line. The formulation bases on expanding dynamic stiffness by using the continued fraction approach. The solution converges rapidly over the whole time range along with the order of the continued fraction increases. In addition, the method is suitable for large scale systems. The numerical method is employed which is a combination of the time domain SBFEM for far field and the finite element method used for near field. By using the continued fraction solution and introducing auxiliary variables, the equation of motion of unbounded domain is built. Applying the spectral shifting technique, the virtual modes of motion equation are eliminated. Standard procedure in structural dynamic is directly applicable for time domain problem. Since the coefficient matrixes of equation are banded and symmetric, the equation can be solved efficiently by using the direct time domain integration method. Numerical examples demonstrate the increased robustness, accuracy and superiority of the proposed method. The suitability of proposed method for time domain simulations of complex systems is also demonstrated.

Removal of aqueous Pb and Cr(VI) by magnetically modified biochars: Effects of feedstock properties and production conditions

Jun Dong,Wanpeng Liu,Xiaofeng Shen,Shengdao Shan 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-

Rapid Analysis of Tetraconazole Residues in Fruits and Vegetables using Ethyl Acetate Extraction and Gas Chromatography-tandem Mass Spectrometry

Xu, Jun,Dong, Fengshou,Liu, Xingang,Li, Jing,Li, Yuanbo,Shan, Weili,Zheng, Yongquan Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.12

A method based on ethyl acetate extraction and gas chromatography with tandem mass spectrometry was developed for determining tetraconazole residues in fruits and vegetables. A 10 g homogenized sample was mixed with 10 mL ethyl acetate, shaken vigorously for 3 min, stored at $-20^{\circ}C$ for 15 min, and then vortexed vigorously for 1 min; 1 g NaCl and 4 g anhydrous $MgSO_4$ were added. The clean-up was carried out by applying dispersive solid-phase with 150 mg $MgSO_4$and 50 mg primary secondary amine. Three precursor product ion transitions for tetraconazole were measured and evaluated to provide the maximum degree of confidence. Average recoveries in fruits and vegetables at three levels (0.005, 0.05 and 0.5 mg/kg) ranged from 85.53% to 110.66% with relative standard deviations ($RSD_r$) from 1.3% to 17.5%. The LODs ranged from 0.002 to 0.004 ${\mu}g$/kg, and LOQs ranged from 0.006 to 0.012 ${\mu}g$/kg. This method was also applied to determine tetraconazole residue in cucumber dissipation experiment under field conditions. The half-lives of tetraconazole in cucumber were in the range of 2.1-3.1 days.